Vascular Endothelial Growth Factor and Semaphorin 3A influence murine thymocyte migration in vitro
-
1
Institute for Experimental Medicine RAMS, Immunology, Russia
Angiogenic Vascular Endothelial Growth Factor (VEGF) and axon guidance molecule Semaphorin 3A (Sema 3A) are known to be produced by thymic epithelial cells (Corbel C. et al., 2007; Lepelletier Y. et al., 2007) but their function in T-lymphocyte development is not known. Both factors have common receptor – Neuropilin 1 which is abundantly expressed on the surface of murine thymocytes. The aim of the study was to evaluate the role of VEGF and Sema 3A in murine thymocyte migration in vitro. With the help of transwell systems we found that Sema 3A at concentration of 100 ng/ml possessed chemorepellent activity towards murine thymocytes while VEGF at the range of 1-100 ng/ml showed no effect. Incubation of thymocytes in the presence of anti-Neuropilin 1 antibodies abolished effect of Sema 3A. We further evaluated thymocyte adhesion to the monolayer of endothelial EA.hy 926 cells, preincubated with Sema 3A or VEGF during 4 hours. VEGF at a concentration of 50 ng/ml increased thymocyte adhesion 1,5 times while Sema 3A had no effect. As a positive control we used 10 ng/ml of TNF-α. Transendothelial thymocyte migration through the monolayer of endothelial cells preincubated with 50-100 ng/ml VEGF during 4 hours was increased 1,9 times as compared to the control cultures while preincubation with Sema 3A did not show any influence. These data suggest that both factors may act on transendothelial migration of thymocytes and their egress to periphery: Sema 3A - directly on thymocytes as chemorepellent while VEGF can modify endothelial cell.
Keywords:
thymocyte,
VEGF,
Semaphorin 3A,
Migration,
Adhesion
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Kisseleva
EP and
Lyamina
IV
(2013). Vascular Endothelial Growth Factor and Semaphorin 3A influence murine thymocyte migration in vitro.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00046
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Ekaterina P Kisseleva, Institute for Experimental Medicine RAMS, Immunology, Saint-Petersburg, 197376, Russia, ekissele@yandex.ru