C. albicans phagocytosis by mast cells via Toll like receptor-2: protection or immune escape?
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1
Bauru School of Dentistry. University of São Paulo., Department of Stomatology, area of Pathology, Brazil
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2
Faculty of Medicine of Ribeirão Preto. University of São Paulo., Department of Pharmacology., Brazil
Candida albicans (C. albicans) is a fungus commonly found in the mucosa of the gastrointestinal tract, including the mouth, and may causecandidiasis, especially in immunosuppression. Mast cells reside in the subepithelial region and actively participate in innate immunity, like one of the first lines of defense against C. albicans. The recognition of this fungus can occur via different receptors, e.g. Toll-like receptor 2 (TLR2), present on the surface of mast cells. Thus, this study evaluated in vitro the relationship of the phagocytosis, intracellular oxide nitric production by mast cells challenged with C. albicans and the involvement of TLR2 in these mechanisms. For this, Murine bone marrow cells (BMMC) wild type (TLR2+ /+) or knockout (TLR2-/-) were cultured in presence of stem cell factor and interleukin-3. After, the BMMCs were challenged with FITC-labeled C. albicans by 60 minutes and the phagocytosis analyzed by confocal laser scanning microscopy. Furthermore, intracellular nitric oxide production was measured used the DAF-FM diacetate and spectrophotometer. Our results showed that BMMC TLR2+ /+ phagocytose C. albicans but did not showed overproduction of the nitric oxide after stimulation with fungus, in relation with the basal production. However, in the absence of the TLR2, the nitric oxide production is increased, while the phagocytosis is reduced, in comparison with wild mouse. Therefore, we conclude that the C. albicans recognition by TLR2 results in the increased phagocytosis, but without nitric oxide stimulous. This can represent an escape way of the fungus of the defense mechanisms developed by mast cells via TLR2.
Keywords:
Mast Cells,
immune escape,
Candida albicans,
TLR2 signaling,
Nitric Oxide
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Pinke
KH,
Lima
HG,
Cunha
FQ and
Lara
VS
(2013). C. albicans phagocytosis by mast cells via Toll like receptor-2: protection or immune escape?.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00054
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mrs. Karen H Pinke, Bauru School of Dentistry. University of São Paulo., Department of Stomatology, area of Pathology, Bauru, Brazil, karen.pinke@gmail.com