The role of P2X7 receptor in hepatic necrosis caused by Plasmodium chabaudi AS infection
-
1
Universidade de Sao Paulo, Immunology, Brazil
The intense activation of the immune system during the erythrocytic stage of Plasmodium is responsible for several syndromes associated with the disease. Purinergic receptors of the P2X family detect extracellular ATP (eATP) and their interaction induces inflammasome activation in macrophages and consequent production of proinflammatory cytokines and cell death. In malaria, ATP is released upon erythrocyte rupture and T cell activation. In the murine model of malaria caused by Plasmodium chabaudi AS, infected erythrocytes preferentially adhere to the liver endothelium. Therefore, it is believed that the rupture of parasitized erythrocytes and consequent release of ATP mainly occur in that organ. A study performed by our research group showed that liver necrosis occurs in C57BL/6 mice infected with P. chabaudi AS.
To study the role of P2X7R in hepatic necrosis, we infected C57BL/6 and P2X7R-/- mice with Plasmodium chabaudi chabaudi AS 106 (i.p.) and 7 days later the livers were collected for histopathological analysis. We observed that infected P2X7R-/- mice, contrary to C57BL/6 mice, do not present/develop liver necrosis. In another approach, we infected DBA/2 mice, which were treated with P2X7-antagonist Brilliant Blue-G (BBG) (45.5 mg/Kg) every 48 hours for 5 days. Seven days after infection the livers were collected for histopathological analysis, peripheral blood and serum were collected for hemoglobin, AST, ALT and LDH measurements. Our results indicate that BBG administration prevents hepatic necrosis.
These data demonstrate that the absence of P2X7 receptor or the administration of BBG ameliorates the severity of hepatic necrosis.
Keywords:
P2X7 receptor,
hepatic necrosis,
Plasmodium chabaudi,
ATP signaling,
Malaria
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
Vieira
FS,
Freitas Do Rosário
A and
D'Império Lima
M
(2013). The role of P2X7 receptor in hepatic necrosis caused by Plasmodium chabaudi AS infection.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00057
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Flavia S Vieira, Universidade de Sao Paulo, Immunology, Sao Paulo, Sao Paulo, Brazil, flavinhafsv@gmail.com