The P2X7 receptor contributes to protection and to ameliorate the clinical manifestations of blood-stage Plasmodium chabaudi malaria
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1
University of São Paulo, Brazil
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2
Federal University of Rio de Janeiro, Brazil
Malaria is a potentially serious infectious disease caused by protozoa parasites of the genus Plasmodium. The blood stage of infection is responsible for all symptoms associated with malaria. Recently, it has been shown that innate immune receptors are able to detect signals released by damaged cells, such as ATP. The P2X7 receptor (P2X7R) detects extracellular ATP and therefore could contribute to activate the immune response to Plasmodium. In this study, we observed that ATP levels in the serum of C57BL/6 mice that were infected with Plasmodium chabaudi AS were significantly higher after the period of rupture of infected red blood cells (iRBCs). Moreover, CD4+ T cells and CD11c+ cells from the spleen of infected mice presented with increased P2X7R-mediated responses to ATP or supernatants from lysed iRBCs, which were measured by the permeability to ethidium bromide. Furthermore, P2X7R-/- (C57BL/6 background) female mice had difficulty in controlling parasitemia and failed to reestablish body temperature and weight after the peak of parasitemia. Accordingly, P2X7R-/- male mice died from day 8 to 15 postinfection. Our results suggest that ATP is released from ruptured iRBC and its recognition by the P2X7R contributes to parasite control and to ameliorate the clinical manifestations of blood-stage P. chabaudi malaria.
Keywords:
Malaria,
P2X7 receptor,
Adenosine Triphosphate,
Plasmodium chabaudi,
Spleen
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Salles
ÉM,
Menezes
M,
Cassado
A,
Amaral
E,
Mendez
S,
Da Silva
HB,
Álvarez
JM,
Coutinho Silva
R and
D'Império Lima
M
(2013). The P2X7 receptor contributes to protection and to ameliorate the clinical manifestations of blood-stage Plasmodium chabaudi malaria.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00060
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Ms. Érika M Salles, University of São Paulo, São Paulo, ---, 05508000, Brazil, erika.salles@gmail.com