How M. leprae Hsp65 influences the immune response in genetically selected aged mice?
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1
Instituto Butantan, Brazil
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2
Hospital Israelita Albert Einstein, Brazil
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3
Universidade Federal de São Paulo (UNIFESP), Immunology Division, Brazil
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4
Instituto Butantan, Brazil
Heat shock proteins may trigger innate immune responses and are involved in immunosenescence and autoimmunity. We characterized some cellular and humoral alterations after intraperitoneal administration of 2.5µg M. leprae Hsp65 in genetically selected mice for High (HIII) or Low (LIII) antibody production (9-months-old) and in its F1 hybrids. Aged HIII female injected with Hsp65 presented a survival decrease of 42% when compared to untreated group (control); no changes in IgG1 or IgG2a anti-Hsp production were observed in HIII and LIII mice. Regarding the cellular changes, aged HIII female Hsp65-group presented amplified frequency in CD4+CD154+CD28+ cells (p<0.01) and reduced percentage of B and activated CD11c+ cells (p<0.01) in the spleen, and increased percentage of CD11c+ and NKG1A/C/E+ cells (p<0.01) in the blood compared to control. Hsp65 acts like an imbalance trigger: post-injection, the aged F1H female Hsp65-group died 2 months after the first death, as observed in aged HIII females; however there was no statistically significance compared with F1H control group. Furthermore, aged F1H and F1L female showed amplified frequency of naïve T cells and CD11c cells in spleen (p<0.001). In conclusion, our results confirm the sex dichotomy (sex effect) of the Hsp65 interference in the immunity of aged mice, becoming evident in females. Next, we will characterize innate immune cells in peritoneal cavity after Hsp65 inoculation; in addition, the role of myeloid-derived suppressor cells will be investigated as these cells are increased during ageing process and have been associated with attenuation of experimental autoimmune diseases. Support: CNPq, FAPESP, INCT-TOX.
Keywords:
Heat shock proteins (HSP),
Aging,
immunosenescence,
genetically selected mice,
Molecular Mimicry
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Baldon
EJ,
Marengo
EB,
Bueno
V,
De Franco
M and
Sant'Anna
OA
(2013). How M. leprae Hsp65 influences the immune response in genetically selected aged mice?.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00061
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mr. Estevam J Baldon, Instituto Butantan, São Paulo, SP, 05503-900, Brazil, ejbaldon@gmail.com