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Morin attenuates airway hyperresponsiveness of allergic asthma via down regulation of immune-inflammatory biomarkers

Amit D. Kandhare1, Pinaki Ghosh1 and Subhash L. Bodhankar1*
  • 1 Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, Dept. of Pharmacology, India

Introduction:
Asthma is immune-inflammatory airway disorder characterized by airway obstruction and hyperresponsiveness. Morin [2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one] is a flavonoid reported to have inhibition of IgE-mediated allergic response in mast cells.

Aim:
Aim of present investigation was to evaluate antiasthmatic activity of morin against ovalbumin (OVA)-induced airway hyperresponsiveness (AHR) in rats.

Material and methods:
AHR was induced in the male Sprague Dawley rats by intraperitoneal injection of OVA. Rats were treated with morin (10, 30 and 100 mg/kg, p.o.) for 28 days followed by OVA-aerosol intranasal challenge. Lung function test was performed. Biochemical and molecular parameters were assessed in blood, bronchoalveolar lavage fluid (BALF) and lung tissue.
Results:
Total cell counts, eosinophil counts in BALF and blood increased significantly (P < 0.001) in AHR control rats whereas treatment with morin (30 and 100 mg/kg, p.o.) significantly reduced cell counts (P < 0.001). Significant decrease (P < 0.001) in spirometry flow and peak inspiratory flow whereas peak expiratory flow and force expired volume was significantly increased (P < 0.001) in morin treated rats. Increased levels of nitric oxide, malondialdehyde, IgE and histamine were significantly (P < 0.001) inhibited by morin. RT-PCR analysis of lung revealed that morin (30 and 100 mg/kg, p.o.) significantly (P < 0.001) suppressed mRNA expression of TNF-α, interleukin (IL)-1β, IL-4, IL-6, COX-2 and leukotriene B4 (LTB-4) in a dose-dependent manner.

Conclusion:
It is concluded that morin has anti-asthmatic effects mediated by reduction of oxidative stress and airway inflammation via down regulation of IgE, COX-2, IL-4, IL-6 and LTB-4 expression.

Acknowledgements

The authors would like acknowledge Dr. S. S. Kadam, Vice-Chancellor and Dr. K. R. Mahadik, Principal, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India, for providing necessary facilities to carry out the study.

References

1. Chen YH, Wu R, Geng B, Qi YF, Wang PP, Yao WZ, et al. Endogenous hydrogen sulfide reduces airway inflammation and remodeling in a rat model of asthma.Cytokine 2009;45(2):117-23.
2. Lee M, Kim S, Kwon OK, Oh SR, Lee HK, Ahn K. Anti-inflammatory and anti-asthmatic effects of resveratrol, a polyphenolic stilbene, in a mouse model of allergic asthma. Int Immunopharmacol 2009;9(4):418-24.
3. Portela Cde P, Massoco Cde O, de Lima WT, Palermo-Neto J. Stress-induced increment on total bronchoalveolar cell count in OVA-sensitized rats. Physiol Behav 2001;72(3):415-20.

Keywords: Airway Hyperresponsiveness, COX-2, IgE, Immune-inflammation, LTB-4, Morin, Ovalbumin

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Immune-mediated disease pathogenesis

Citation: Kandhare AD, Ghosh P and Bodhankar SL (2013). Morin attenuates airway hyperresponsiveness of allergic asthma via down regulation of immune-inflammatory biomarkers. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00063

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Received: 08 Mar 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Subhash L Bodhankar, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, Dept. of Pharmacology, Pune, Maharashtra, 411038, India, sbodh@yahoo.com