Role of P2X7 receptor in the response of phagocytes to Plasmodium chabaudi infection
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1
University of São Paulo, Brazil
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2
Federal University of Rio de Janeiro, Brazil
Malaria is characterized by intense activation of the immune system that seems to contribute to protection and pathogenesis. ATP recognition by P2X7R in immune cells is important for cell activation and death. In this study, we evaluated the effects of P2X7R-mediated signaling in phagocytes during infection with Plasmodium chabaudi. C57BL/6 and P2X7R-/- mice were infected with P. chabaudi infected red blood cells (iRBC) and clinical parameters were measured. Kinetics of phagocytic cells and cell death in the spleen of infected animals was determined by flow cytometry. In vitro assay was performed with bone marrow derived macrophages (BMDM) from both mice strains in presence of iRBC and ATP. Between days 9 and 17 post-infection, 80% of P2X7R-/- mice died, while the C57BL/6 group presented no death. Clinical manifestations and their improvement were delayed in knockout mice in comparison to wild-type. P2X7R-/- mice showed a higher number of CD11b+ cells at day 7 post-infection compared to the wild-type mice. The percentage of cell death found in spleen in the same day was lower in knockout group. Moreover, BMDM from C57BL/6 mice became significantly more sensitive to ATP when exposed previously to iRBC in a P2X7-dependent way when compared to that without iRBC. Thus, mice lacking the P2X7R seem to be more susceptible to P. chabaudi infection, presenting more severe clinical manifestations. Furthermore, the elevated number of phagocytes in spleen from P2X7R-/- mice when compared to C57BL/6 mice could be due to the reduced rate of cell death found in those cells.
Acknowledgements
Fapesp and CNPq
Keywords:
P2X7,
Malaria,
Phagocytes,
ATP,
Plasmodium
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Menezes
M,
Salles
ÉM,
Cassado
A,
Amaral
E,
Da Silva
HB,
Mosig
J,
Silva
R and
D'Império Lima
M
(2013). Role of P2X7 receptor in the response of phagocytes to Plasmodium chabaudi infection.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00073
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Miss. Maria Menezes, University of São Paulo, São Paulo, Brazil, menezesnmaria@gmail.com