Specific TCD8+IL-10+ and TCD8+TGF-beta+ cells in active tuberculosis patients
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1
Universidade Federal de Goiás, Departamento de Microbiologia, Imunologia, Patologia e Parasitologia, Brazil
Tuberculosis (TB) is responsible for causing over 1.5 million deaths each year. TCD4+ together with TCD8+ cells and their cytokines are important for protective immune response to TB. Although TCD8+ cells has been studied in TB, several aspects is not yet clarified. In the absence of TCD4+ cells, TCD8+ cells increase their cytotoxic function and production of different cytokines in order to respond to Mtb infection. To understand the role of TCD8+ cells as regulatory cytokine producers in TB, peripheral blood TCD8+ cells were characterized and compared their profile with healthy controls (TST-). PBMC from 15 patients with active pulmonary TB (mean age = 40.5) and 15 healthy controls TST-, matched by sex and age to TB patients, were cultivated for 96 hours with Mtb recombinant antigen (rMPT-51). TCD8+ cell subsets were analyzed by flow cytometry. TB patients presented higher levels of TCD8+IL-10+ (medium=1.2±0.9; PHA=12.8±1.5; rMPT-51=5.0±1.4) and TCD8+TGF-beta+ (medium=1.6±1.3; PHA=14.1±1.7; rMPT-51=8.0±1.1) in response to MPT-51 than TST- individuals (TCD8+IL-10, medium=1.3±1.1; PHA=11.9±1.7; rMPT-51=3.0±1.5); (TCD8+TGF-beta+, medium=2.4±0.8; PHA=13.6±2.3; rMPT-51=2.8±1.2). The cytotoxic markers of TCD8+ cell such as Perforin and Granzime-B are independent of the MPT-51 stimulus, and TB patients had lower percentage of triple positive cells than the healthy controls (TST-=21.7±2.8; TB=9.1±1.3). Conclusions: Patients with TB presented Mtb specific TCD8+ positive for IL-10 and TGF-beta, whereas presented lower cytotoxic markers. These results suggest that TCD8+ cells can contribute to the regulatory immune response in active tuberculosis.
Acknowledgements
Financial Support: CNPq; CAPES.
Keywords:
Tuberculosis,
TCD8+ cells,
rMPT-51,
cytotoxic activity,
regulatory cells
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Silva
BS,
Trentini
MM,
Costa
AC,
Costa-Júnior
AO,
Kipnis
A and
Junqueira-Kipnis
A
(2013). Specific TCD8+IL-10+ and TCD8+TGF-beta+ cells in active tuberculosis patients.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00094
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mrs. Bruna Daniella S Silva, Universidade Federal de Goiás, Departamento de Microbiologia, Imunologia, Patologia e Parasitologia, Goiânia, Goiás, 74605050, Brazil, bruna.daniella@hotmail.com