Activity Correlation Imaging: Visualizing Function and Structure of Neuronal Populations
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1
Georg-August University of Göttingen, Dep. of Neurophysiology and Cellular Biophysics, Germany
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2
Max Planck Institute for Brain Research, Germany
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3
HHMI Janelia farm research campus, United States
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4
DFG Research Center for Molecular Physiology of the Brain, Germany
It is often desirable for the investigation of neuronal networks to simultaneously measure the individual neuron's activities and their morphology. Because of the highly correlated activity in interconnected dendritic trees, it is possible to extract their morphology exclusively from measurements of their temporal activity patterns. Morphological data of different trees is then combined into a high-contrast, multicolor visualization of the neuronal network, having used only a single functional dye. This novel technique, called activity correlation imaging [1], is demonstrated in the Xenopus laevis olfactory bulb, showing both activities of the mitral/tufted cells as well as their projections into the olfactory glomeruli.
We thereby show that mostly distant mitral cells project dendrites into the same glomerulum, that these mitral cells are coupled through gap junctions and that they perform synchronous activity.
References
[1] Junek S, Chen T-W, Alevra M, Schild D (2009) Activity correlation imaging: visualizing function and structure of neuronal populations. Biophys. J., 96, 3801-3809.
Keywords:
activity correlation imaging
Conference:
Tenth International Congress of Neuroethology, College Park. Maryland USA, United States, 5 Aug - 10 Aug, 2012.
Presentation Type:
Invited Symposium (only for people who have been invited to a particular symposium)
Topic:
Novel Tools and Methods
Citation:
Alevra
M,
Junek
S,
Chen
T and
Schild
D
(2012). Activity Correlation Imaging: Visualizing Function and Structure of Neuronal Populations.
Conference Abstract:
Tenth International Congress of Neuroethology.
doi: 10.3389/conf.fnbeh.2012.27.00035
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Received:
29 Apr 2012;
Published Online:
07 Jul 2012.
*
Correspondence:
Mr. Mihai Alevra, Georg-August University of Göttingen, Dep. of Neurophysiology and Cellular Biophysics, Göttingen, 37073, Germany, malevra@gwdg.de