Event Abstract

Effect of exhaustive ultra-endurance vs moderate exercise on rodent cerebellum antioxidant capacity

  • 1 Federal University of Pernambuco, Brazil
  • 2 Federal University of Alagoas, Brazil

Ultra-endurance (UE) running increases O2 consumption and reactive oxygen species production in peripherical systems but it is still unknown how this type of training can affect brain regions involved in the movement control. The present study test the hypothesis that in addition to muscle damage, the cerebellum redox balance can be impaired by increasing training volumes. Forty five adult male Wistar rats were randomly subdivided in 6 groups according to the moderate training period followed or not by exhaustion test (ET) : control(C), Control + ET, moderate training volume (MV) moderate training volume + ET (MV-ET), high training volume (HV) and high training volume + ET (HV-ET). A continuous running characterized by low to moderate intensity was performed 5 times a week on a treadmill. The training period was gradually increased to 30 (MV) and 90 (HV) min/day over 12-week period. The velocity during ET, and the serum concentration of lactate (Lac), Creatine Kinase (CK) lactate dehydrogenase (LDH) and corticosterone were assessed. The cerebellum redox balance was analyzed by lipoperoxidation (LP) levels, reduced/ oxidized Glutathione GSH/GSSG ratio and the superoxide dismutase (SOD) and catalase (CAT) activities. Serum lactate levels were reduced in the MV-ET and HV-ET compared to CT-ET group (p<0.05) while CK levels were increased in the MV-ET, HV-ET and HV groups (p<0.03). Corticosterone concentration was higher in the HV and HV-ET contrast to MV-ET and C-ET groups (p≤0.05). Higher LP levels in the cerebellar tissue were induced by HV training despite the presence of ET. CAT activity was higher in the MV group compared to MV-ET, HV-ET and C groups (p<0.02). On the other hand, increased SOD activity was seen after ET independent of the running volume compared to C group (p≤0.05). Running velocity, GSH/GSSG ratio in the cerebellum, serum LDH and NO levels were not modified by the training. The data shows that the resilience of cerebellum to oxidative damage can be kept under moderate training volume but it is reduced by high volume in ultra-endurance running especially due to unbalanced antioxidant enzymatic activity. Keywords: Oxidative stress; central nervous system; exercise volume; catalase; superoxide dismutase; lipoperoxidation.

Acknowledgements

Coordination for the Improvement of Higher Level or Education Personnel (CAPES, Finance Code 001 and PROCAD-2013).

Keywords: Oxidative stress ., Lipoperoxidation, superoxide dismutase ., Catalase, Central Nervous System

Conference: XVI Meeting of the Portuguese Society for Neuroscience (SPN2019), Lisboa, Portugal, 30 May - 1 Jun, 2019.

Presentation Type: Poster presentation

Topic: Cellular and Molecular Neurosciences

Citation: De Souza RF, Augusto RL, Moraes SR, Vanessa L, Pereira DD, Moreno GM, Souza FB, Souza FM and Andrade- Da- Costa BL (2019). Effect of exhaustive ultra-endurance vs moderate exercise on rodent cerebellum antioxidant capacity. Front. Cell. Neurosci. Conference Abstract: XVI Meeting of the Portuguese Society for Neuroscience (SPN2019). doi: 10.3389/conf.fncel.2019.01.00018

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Received: 26 Feb 2019; Published Online: 27 Sep 2019.

* Correspondence: Prof. Raphael F De Souza, Federal University of Pernambuco, Recife, Brazil, raphaelctba20@hotmail.com

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