Event Abstract

Back to Event

SPATIAL DISTRIBUTION OF FIBROBLAST GROWTH FACTOR RECEPTOR 2 IN NORMAL AND LESIONED CNS OF
PLEURODELES WALTLII

Marie Z. MOFTAH1, 2, 3*, Marc Landry2, 3, Frédéric Nagy2, 3 and Jean-Marie Cabelguen4
  • 1 Faculty of Science, Alexandria University, Alexandria, Zoology Department, Egypt
  • 2 Central Mechanisms of Pain Sensitization, Bordeaux, IINS, CNRS UMR 5297, France
  • 3 IINS,CNRS UMR 5297, Central Mechanisms of Pain Sensitization, Bordeaux, Université de Bordeaux, France
  • 4 INSERM U862, Neurocentre Magendie, Pathophysiology of Spinal Networks, Bordeaux, Université de Bordeaux, France

Fibroblast Growth Factors (FGFs) have been implicated in numerous cellular processes including proliferation, migration, differentiation and neuronal survival. One of these growth factors, Fibroblast Growth Factor 2 (FGF2), is apparently implicated in the ability of the adult salamander (Pleurodeles waltlii) to recover locomotion following complete transection of the spinal cord. In a previous study, we reported up regulation of FGF2 during regeneration of damaged axons and recovery of hind limb locomotion. In this study reported here, we investigated the spatial distribution of FGFR2-one of the receptors that mediate the effects of FGF2-using a variety of techniques, namely, western blot, immunohistochemistry and in situ hybridization. We find that in intact animals FGFR2 is mainly expressed in the most posterior part of body spinal cord (SC3) specifically in neurons. However, lesioning the spinal cord produces increased expression in Brainstem (BS) neurons and decreased expression in posterior parts of the spinal cord not only in neurons but also in the neuroglial ependymal cells lining the central canal. This suggests that FGF2 simultaneously activates FGFR1 and 2, perhaps at different points in the regeneration process and thus FGFR2 might play at least an indirect role in the spontaneous regeneration observed in this species and might be relevant to the treatment of spinal cord lesions in humans. Verification of this possibility will require studies of additional time points.

Figure 7
Image

Acknowledgements

This study was supported by «Région Aquitaine» contract no. 20040301208N and N€uromed FP7 project no. 245807. Postdoctoral grants were offered to MM by the AUF (Agence Universitaire de la Francophonie) and the Fondation Singer Polignac.

References

Chevallier, S., M. Landry, F. Nagy and J.M. Cabelguen, 2004. Recovery of bimodal locomotion in the spinal-transected salamander, Pleurodeles waltlii. Eur. J. Neurosci., 20: 1995-2007. DOI: 10.1111/j.1460-9568.2004.03671.x
Davis, B.M., J.L. Ayers, L. Koran, J. Carlson and M.C. Anderson et al., 1990. Time course of salamander spinal cord regeneration and recovery of swimming: HRP retrograde pathway tracing and kinematic analysis. Exp. Neurol., 108: 198-213. http://dx.doi.org/10.1016/0014-4886(90)90124-B
Dionne, C.A., G. Crumley, F. Bellot, J.M. Kaplow and G. Searfoss et al., 1990. Cloning and expression of two distinct high-affinity receptors cross-reacting with acidic and basic fibroblast growth factors. Embo J., 9: 2685-2692. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC551973/
Eckenstein, F.P., K. Kuzis, R. Nishi, W.R. Woodward and C. Meshul et al., 1994. Cellular distribution, subcellular localization and possible functions of basic and acidic fibroblast growth factors. Biochem Pharmacol, 47: 103-110. http://dx.doi.org/10.1016/0006-2952(94)90442-1
Keegan, K., D.E. Johnson, L.T. Williams and M.J. Hayman, 1991. Isolation of an additional member of the fibroblast growth factor receptor family, FGFR-3. Proc Natl Acad Sci. U S A 88: 1095-1099. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC50963/
Landry, M., K. Holmberg, X. Zhang and T. Hokfelt, 2000. Effect of axotomy on expression of NPY, galanin and NPY Y1 and Y2 receptors in dorsal root ganglia and the superior cervical ganglion studied with double-labeling in situ hybridization and immunohistochemistry. Exp. Neurol., 162: 361-384. http://dx.doi.org/10.1006/exnr.1999.7329
Landry, M., R. Bouali-Benazzouz, S. El Mestikawy, P. Ravassard and F. Nagy, 2004. Expression of vesicular glutamate transporters in rat lumbar spinal cord, with a note on dorsal root ganglia. J. Comparative Neurology 468: 380-394. DOI: 10.1002/cne.10988
Moftah, M., M. Landry, F. Nagy and J.M. Cabelguen, 2008. Fibroblast growth factor-2 mRNA expression in the brainstem and spinal cord of normal and chronic spinally transected urodeles. J. Neurosci. Res., 86: 3348-3358. DOI: 10.1002/jnr.21776
Moftah, M.Z., S.A. Downie, N.B. Bronstein, N. Mezentseva and J. Pu et al., 2002. Ectodermal FGFs induce perinodular inhibition of limb chondrogenesis in vitro and in vivo via FGF receptor 2. Dev. Biol., 249: 270-282. http://dx.doi.org/10.1006/dbio.2002.0766
Piatt, J., 1955. Regeneration of the spinal cord in the salamander. J. Exp. Zool 29:177-208. DOI: 10.1002/jez.1401290109
Zhang, F., J.D. Clarke and P. Ferretti, 2000. FGF-2 Up-regulation and proliferation of neural progenitors in the regenerating amphibian spinal cord in vivo. Developmental Biology 225: 381-391. DOI: 10.1006/dbio.2000.9843
Zhang, F., P. Ferretti and J.D. Clarke, 2003. Recruitment of postmitotic neurons into the regenerating spinal cord of urodeles. Dev. Dyn., 226: 341-348. DOI: 10.1002/dvdy.10230

Keywords: Spinal lesion, In Situ Hybridization, brainstem, Spinal Cord, Regeneration

Conference: 4th Conference of the Mediterrarnean Neuroscience Society, Istanbul, Türkiye, 30 Sep - 3 Oct, 2012.

Presentation Type: Full Publication

Topic: Abstracts

Citation: MOFTAH MZ, Landry M, Nagy F and Cabelguen J (2013). SPATIAL DISTRIBUTION OF FIBROBLAST GROWTH FACTOR RECEPTOR 2 IN NORMAL AND LESIONED CNS OF
PLEURODELES WALTLII. Conference Abstract: 4th Conference of the Mediterrarnean Neuroscience Society. doi: 10.3389/conf.fnhum.2013.210.00052

Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.

The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.

Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.

For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.

Received: 25 Jan 2013; Published Online: 11 Apr 2013.

* Correspondence: Prof. Marie Z MOFTAH, Faculty of Science, Alexandria University, Alexandria, Zoology Department, Alexandria, 21151, Egypt, marie.moftah@alexu.edu.eg