Exposure to Inhalational Anaesthetics Causes Neuroinflammation
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1
Monash University, School of Psychology and Psychiatry, Australia
Anaesthetics ensure the loss of awareness and analgesia during surgical procedures in patients. Recent hypotheses suggest that anaesthesia may be the driving force in post-operative cognitive disorder (POCD), possibly via the induction of neuroinflammation. The present study is first to examine whether isoflurane and sevoflurane enhance neuroinflammation in six defined regions of the mouse brain. Thirty-six male C57/BL6J mice (aged 8-10 weeks) were divided into five conditions: control saline (n=10), positive control lipopolysaccharide (LPS) (n=10), 1 h isoflurane with 6 hour survival (n=4), 1 h isoflurane with 24 hours survival (n=6), 1 h sevoflurane with 24 hours survival (n=6). Neuroinflammation was analysed using immunohistochemistry for glial fibrillary acidic protein (GFAP) and S100β for astrocytes, and CD11b (Cluster of differentiation 11b) for microglia. Mice were transcardially perfused with 4% paraformaldehyde and brains were sectioned coronally. Immunohistochemistry was performed and marker expression was examined using light microscopy and densitometric analysis. LPS, isoflurane and sevoflurane significantly increased the expression of neuroinflammatory markers in several brain regions. GFAP and S100β were significantly upregulated at both survival times and in all four treatment groups (p<.05), whereas CD11b was only significantly upregulated 24 hours after anaesthesia (p<.05). The present findings indicate that these isoflurane and sevoflurane cause neuroinflammation, first in astrocytes and later in microglia. This neuroinflammation may provide the neurobiological basis of POCD.
Keywords:
Neuroinflammation,
S100B,
Glial Fibrillary Acidic Protein,
CD11b,
Isoflurane,
sevoflurane,
Post Operative Cognitive Disorder
Conference:
ACNS-2012 Australasian Cognitive Neuroscience Conference, Brisbane, Australia, 29 Nov - 2 Dec, 2012.
Presentation Type:
Poster Presentation
Topic:
Other
Citation:
Zaman
SM and
Robinson
SR
(2012). Exposure to Inhalational Anaesthetics Causes Neuroinflammation.
Conference Abstract:
ACNS-2012 Australasian Cognitive Neuroscience Conference.
doi: 10.3389/conf.fnhum.2012.208.00159
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Received:
26 Oct 2012;
Published Online:
17 Nov 2012.
*
Correspondence:
Miss. Sasha M Zaman, Monash University, School of Psychology and Psychiatry, Melbourne, Victoria, 3800, Australia, sasha.zaman@gmail.com