AUTHOR=Ahmadi Hamed, Rodehutscord Markus, Siegert Wolfgang TITLE=Bi-objective optimization of nutrient intake and performance of broiler chickens using Gaussian process regression and genetic algorithm JOURNAL=Frontiers in Animal Science VOLUME=4 YEAR=2023 URL=https://www.frontiersin.org/articles/10.3389/fanim.2023.1042725 DOI=10.3389/fanim.2023.1042725 ISSN=2673-6225 ABSTRACT=This study investigated whether quantifying the trade-off between the maxima of two response traits increases the accuracy of diet formulation. To achieve this, average daily weight gain (ADG) and gain:feed ratio (G:F) responses of 7–21-day-old broiler chickens to the dietary supply of three nutrients (intake of digestible glycine equivalents, digestible threonine, and total choline) were modeled using a newly developed hybrid machine learning-based method of Gaussian process regression and genetic algorithm. The dataset comprised 90 data lines. Model-fit-criteria indicated a high model adjustment and no prediction bias of the models. The bi-objective optimization scenarios through Pareto front revealed the trade-off between maximized ADG and maximized G:F and provided information on the needed input of the three nutrients that interact with each other to achieve the trade-off scenarios. The trade-off scenarios followed a nonlinear pattern. This indicated that choosing target values intermediate to maximized ADG and G:F after single-objective optimization is less accurate than feed formulation after quantifying the trade-off. In conclusion, knowledge of the trade-off between maximized ADG and maximized G:F and the needed nutrient inputs will help feed formulators to optimize their feed with a more holistic approach.