AUTHOR=Scarritt Michelle E. , Pashos Nicholas C. , Bunnell Bruce A. TITLE=A Review of Cellularization Strategies for Tissue Engineering of Whole Organs JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=3 YEAR=2015 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2015.00043 DOI=10.3389/fbioe.2015.00043 ISSN=2296-4185 ABSTRACT=

With the advent of whole organ decellularization, extracellular matrix scaffolds suitable for organ engineering were generated from numerous tissues, including the heart, lung, liver, kidney, and pancreas, for use as alternatives to traditional organ transplantation. Biomedical researchers now face the challenge of adequately and efficiently recellularizing these organ scaffolds. Herein, an overview of whole organ decellularization and a thorough review of the current literature for whole organ recellularization are presented. The cell types, delivery methods, and bioreactors employed for recellularization are discussed along with commercial and clinical considerations, such as immunogenicity, biocompatibility, and Food and Drug Administartion regulation.