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Front. Bioeng. Biotechnol. | doi: 10.3389/fbioe.2019.00347

Comparative metabolic responses induced by pyridine and imidazole in Blakeslea Trispora

 Yang Liu1, 2, 3, Xiang-Yu Li3,  Shu H. Lu3,  Chao Yu3, Yu-Zhou Zhang1, 2,  Zhi-Ming Wang3* and  Jian m. Yao1*
  • 1Institute of Plasma Physics, Hefei Institutes of Physical Science (CAS), China
  • 2University of Science and Technology of China, China
  • 3Cabio Biotech (Wuhan) Co., Ltd, China

Lycopene cyclase need to be inhibited by the blockers like pyridine or imidazole in the lycopene biosynthesis of B.Trispora. This work investigated how pyridine and imidazole impacted the basal metabolism of B.Trispora, especially imidazole had been used as the industrial blocker, thus the results helped us understand how imidazole could affect the lycopene production and application, and wonder if pyridine could be another choice of blocker in industry. The samples of different fermentation modes were taken every 12 hours and detected by GC-MS, then the data of materials were typed into SPSS 16.0, and analyzed by the method of Principal Component Analysis (PCA). 38 intracellular meaningful metabolites were analyzed in the lycopene process with imidazole, while only 14 significant metabolites were identified in the pyridine process. It was found that amino acids, fatty acids, organic acids including phosphoric acid, carbon source and imidazole derivatives explained the most variance in imidazole fermentation, correspondingly, fatty acids, carbon source and pyridine derivatives played important roles in pyridine fermentation, and above all, the residual of both blockers’ derivatives would bring the potential risks on applications of lycopene products. Predominantly, durene met 0.35µg/mg DCW with imidazole and piperidine formaldehyde attained 0.24µg/mg DCW with pyridine after the end of lycopene fermentations.

Keywords: Metabolism, LYCOPENE, pyridine, imidazole, Blakeslea trispora

Received: 08 Aug 2019; Accepted: 06 Nov 2019.

Copyright: © 2019 Liu, Li, Lu, Yu, Zhang, Wang and Yao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Zhi-Ming Wang, Cabio Biotech (Wuhan) Co., Ltd, Wuhan, 430020, Hubei Province, China,
Dr. Jian m. Yao, Institute of Plasma Physics, Hefei Institutes of Physical Science (CAS), Hefei, 230031, Anhui Province, China,