%A Zheng,Huimin %A Tian,Yujuan %A Gao,Qian %A Yu,Yingjie %A Xia,Xianyou %A Feng,Zhipeng %A Dong,Feng %A Wu,Xudong %A Sui,Lei %D 2020 %J Frontiers in Bioengineering and Biotechnology %C %F %G English %K topography,Adhesion,Osteogenic differentiation,Integrin α2,Pi3k-akt %Q %R 10.3389/fbioe.2020.00463 %W %L %M %P %7 %8 2020-May-19 %9 Original Research %# %! Micro-nano topography promotes osteogenic differentiation %* %< %T Hierarchical Micro-Nano Topography Promotes Cell Adhesion and Osteogenic Differentiation via Integrin α2-PI3K-AKT Signaling Axis %U https://www.frontiersin.org/articles/10.3389/fbioe.2020.00463 %V 8 %0 JOURNAL ARTICLE %@ 2296-4185 %X Surface topography dictates important aspects of cell biological behaviors. In our study, hierarchical micro-nano topography (SLM-AHT) with micro-scale grooves and nano-scale pores was fabricated and compared with smooth topography (S) and irregular micro-scale topography (SLA) surfaces to investigate mechanism involved in cell-surface interactions. Integrin α2 had a higher expression level on SLM-AHT surface compared with S and SLA surfaces, and the expression levels of osteogenic markers icluding Runx2, Col1a1, and Ocn were concomitantly upregulated on SLM-AHT surface. Moreover, formation of mature focal adhesions were significantly enhanced in SLM-AHT group. Noticablely, silencing integrin α2 could wipe out the difference of osteogenic gene expression among surfaces with different topography, indicating a crucial role of integrin α2 in topography induced osteogenic differentiation. In addition, PI3K-AKT signaling was proved to be regulated by integrin α2 and consequently participate in this process. Taken together, our findings illustrated that integrin α2-PI3K-AKT signaling axis plays a key role in hierarchical micro-nano topography promoting cell adhesion and osteogenic differentiation.