Cu3BiS3 Nanocrystals as Efficient Nanoplatforms for CT Imaging Guided Photothermal Therapy of Arterial Inflammation

Cardio-cerebrovascular diseases caused by chronic inflammatory atherosclerosis seriously damage human health. Nano-photothermal technology has been proven to inhibit the development of vascular inflammation, but the currently reported photothermal agents cannot efficient monitor it during the development of the disease. Herein, we designed and prepared an efficient bifunctional nanoplatform for CT imaging guided photothermal therapy of arterial inflammation. Cu3BiS3 nanocrystals with a size of about 12 nm were synthesized by a simple hydrothermal method. The as-prepared Cu3BiS3 nanocrystals showed intense absorption in the NIR region, thus exhibited amazing photothermal effect. The photothermal conversion efficiency of Cu3BiS3 nanocrystals was reach up to 58.6% under the excitation of an 808 nm laser with a power density of 0.4 W cm–2. Cu3BiS3 nanocrystals can efficiently kill the macrophages both in vitro and in vivo, which plays an important role in the development of atherosclerosis, thus can be used as an effective way to inhibit the occurrence of hypertension. Importantly, Cu3BiS3 nanocrystals can be used as an efficient CT contrast agent to monitor carotid inflammation. Our work provides an insight for imaging guided photothermal therapy of arterial inflammation.


INTRODUCTION
Atherosclerosis (AS) is one of the cardiovascular diseases which are the leading cause of human death (Libby et al., 2011;Tzoulaki et al., 2016). Macrophages are the key cells that play an important role in AS formation. Nanoparticle based photothermal therapy (PTT) exhibits a very promising potential due to its less side effects which can kill macrophages resulted from the photothermal effect of nanoparticles, comparing to the traditional whole body chemotherapy, and stent implant surgery having in-stent restenosis risks (Tabas, 2010;Wang et al., 2019). With the development of nanotechnology, PTT technology as an emerging minimally invasive technology shows great potential for the diagnosis and treatment of atherosclerosis . Photothermal therapy technology is to use photothermal materials with good optical property of near-infrared (NIR) absorption under laser irradiation to achieve local high temperature, thereby effectively killing cells at the lesion site with no surgery (Li et al., 2013(Li et al., , 2017Song et al., 2017). The NIR (700-1400 nm) laser is an important NIR light source widely used in phototherapy which has very strong penetration ability to biological tissues (Liu et al., 2016). During the progression of atherosclerosis, massive infiltration of monocytes/macrophages and other inflammatory cells and the pathological proliferation of vascular smooth muscle cells promotes plaque formation, secondary stenosis and even occlusion, leading to the occurrence of ischemic diseases (Wang et al., 2019;Zhang et al., 2019). Similar to cancer cells, hyperthermia can also be used to inhibit or kill the aforementioned pathologically expanded cells. Although this minimally invasive technique is widely used in the treatment of cancer, there are few reports on its application to atherosclerosis, and the application of photothermal therapy technology to atherosclerosis has important research significance.
Nano-photothermal technology, especially NIR-laser-driven photothermal therapy technology, has been rapidly developed in recent years. If photothermal treatment technology is to be applied to the treatment of atherosclerosis, the main issue is to explore photothermal agents with low toxicity, multifunction and high photothermal effect Li et al., 2014;Shao et al., 2016;Jin et al., 2018). This is consistent with the application of photothermal therapy technology in cancer treatment. Photothermal agents reported to date mainly include carbon materials, organic materials, metal materials and semiconductor materials . Gold nanostructures are the most extensively and thoroughly studied photothermal agents, but the morphology of noble metal nanostructures will obviously change under the continuous irradiation by lasers which ultimately affect their photothermal properties (Tian et al., 2013). Also, precious metals' price is also one of the issues to restrict the development of such photothermal agents. Organic compounds mainly include nanoparticles of dyes, polyaniline and polypyrrole Li and Pu, 2019). This type of photothermal conversion material is biodegradable, but easily photodegradable or photobleached. Carbon photothermal materials, including graphene and carbon nanotubes, have stable performance, but low light absorption coefficient (Robinson et al., 2010(Robinson et al., , 2011. Semiconductor photothermal nanomaterials show several advantages, such as low price, simple synthesis, high absorption coefficient, stable photothermal performance, and easy functionalization (Meng et al., 2016;Ni et al., 2017;Tan et al., 2017).
Ternary semiconductor nanomaterials can inherit the properties of the corresponding binary semiconductors and produce new characteristics (Liu et al., 2016;Zhou et al., 2016;Li et al., 2017). In particular, copper-based bimetallic sulfides containing elements with imaging properties (such as copper iron sulfur, copper cobalt sulfur, copper manganese sulfur, and copper bismuth sulfur) can theoretically be used as both photothermal agents and imaging contrast agents. Moreover, since the valence of copper in the bimetallic copper-based sulfide is mainly monovalent, it is easily oxidized to divalent and thus degraded in the body (Liu et al., 2016). Therefore, the bimetallic copper-based sulfide is expected to be an ideal photothermal diagnostic agent. However, the previously reported bimetallic sulfides do not have plasmon resonance near-infrared absorption properties. The main reason is that these compounds do not have defective structures. The Cu 3 BiS 3 nanocrystals designed and synthesized by Hu et al. used these materials for the first time in the diagnosis and treatment of cancer (Ai et al., 2011). By adjusting the copper defects of Cu 3 BiS 3 nanocrystals, the as-prepared Cu 3 BiS 3 nanocrystals can simultaneously exhibit intense NIR absorption properties like Cu 2−x S nanocrystals due to copper defects, and also possess CT imaging capability due to the inherently high X-ray attenuation coefficient of bismuth. However, the size of Cu 3 BiS 3 nanocrystals is difficult to adjust. The resulting nanocrystals are large in size and have poor photothermal effects (Hessel et al., 2011;Li et al., 2017). Thus the nanocrystals are easily restricted to the kidneys after being injected into the mice via the tail vein. Reducing its size can effectively improve its photothermal performance, and shorten the time in the body (Liu et al., 2016). It has been reported that adjusting the morphological structure or size of materials can improve the MRI/CT imaging performance (Ai et al., 2011;Lee et al., 2012). Therefore, the key to solving the unsatisfactory photothermal/imaging performance of bimetallic sulfide lies in the fine-tuning synthesis of the defect degree, morphology and particle size of bimetallic copper-based sulfide nanomaterials.
In this work, we designed and prepared Cu 3 BiS 3 nanocrystals with a size of about 12 nm by a modified hydrothermal method in the presence of ethylenediamine. Cu 3 BiS 3 nanocrystals showed intense absorption in the NIR region, thus exhibited excellent photothermal performance. The photothermal conversion efficiency of Cu 3 BiS 3 nanocrystals reached up to 58.6% under the irradiation of an 808 nm laser at a power density of 0.4 W cm −2 . What's more, Cu 3 BiS 3 nanocrystals can efficiently kill the macrophages both in vitro and in vivo, which plays an important role in the development of atherosclerosis, thus can be used as an effective way to inhibit the occurrence of hypertension. In addition, Cu 3 BiS 3 nanocrystals can be used as an efficient CT contrast agent for carotid inflammation. As far as we know, this is first work on Cu 3 BiS 3 nanocrystals for CT imaging guided photothermal therapy of arterial inflammation.

Synthesis of Cu 3 BiS 3 Nanocrystals
Cu(NO 3 ) 2 (1 mmol), Bi(NO 3 ) 3 (1 mmol), and Sodium dimethyldithiocarbamate (3 mmol) and polyvinylpyrrolidone (PVP, 100 mg) were fully dissolved in deionized (DI, 40 mL) water under stirring, ethylenediamine (100 µL) was then added. The precursor solution was then transferred to a PTFE hydrothermal reactor, and kept at 200 • C for 24 h. Black products could be obtained by centrifuge. The products were washed with ethanol and deionized water three times.

Characterization
Transmission electron microscope was used to detect the shape and size of Cu 3 BiS 3 nanocrystals. UV-vis spectrophotometer was used to detect the absorption spectrum of Cu 3 BiS 3 nanocrystals. X-ray photoelectron spectrometer was used to analyze the electronic spectrum of Cu 3 BiS 3 nanocrystals. X-ray diffractometer was used to detect the phase of Cu 3 BiS 3 nanocrystals. Inductively coupled plasma emission spectrometer was used to test the concentration of released ions. 808 nm lasers were used as the light source.

Cell Culture
Raw264.7 macrophage cells was routinely digested and centrifuged. After removing the supernatant, DMEM high glucose complete medium was added to resuspend the cells. The resuspended cells were incubated in a petri dish at a density of 1 × 10 5 /cm 2 and continue culturing in an incubator (37 • C, 5% CO 2 ). The cells were digested with trypsin, and continued to expand when the degree of cell fusion reaches 80%.

CCK-8 Cell Viability Test
Raw264.7 cells were incubated in a 96-well plate. After the cell fusion reached 80%, cells were incubated for 12 h with Cu 3 BiS 3 nanocrystals with different concentrations (0, 10, 20, 40, 80, 160, 320 ppm). Then the Raw264.7 cells were excited by an 808nm laser (0.3 W/cm 2 , 5 min). Then the medium was removed and the cells were washed with PBS three times to prepare CCK-8 working solution (the ratio of CCK-8 reagent to medium is 1:10). After that, CCK-8 working solution (100 µL) was added to each well. After 1 h, a multi-functional microplate reader was used to detect the absorbance at 450nm wavelength, and the analysis data was collected.

Live/Dead Cell Staining
The cultured Raw264.7 cells were collected and inoculated in a 96-well plate and in an incubator (37 • C, 5% CO 2 ). When the degree of cell fusion reached 80%, the cells were incubated with or without Cu 3 BiS 3 nanocrystals in high glucose medium for 12 h. The cells were divided into different groups: blank control group (Control); Cu 3 BiS 3 nanocrystals combined with 808 nm laser at different power density (0.1, 0.2, 0.4 W/cm 2 ). After the treatments, the culture supernatant was removed, washed with PBS for three times. CalceinAM and PI were then added, and incubated in a 37 • C incubator for 20 min. Then the cells were observed under an inverted fluorescence microscope.

Animal Model Construction
All animal experiments were approved by the Animal Ethics Committee of The First Affiliated Hospital of Bengbu Medical College. 28 of 8-week-old ApoE-/-mice were selected and fixed on the rat board after anesthetized with chloral hydrate. The left carotid artery was exposed under the microscope via making a longitudinal incision in the left neck. A 5 mm length silicone tube was placed around the left carotid artery to wrap it around the left carotid artery. The silk thread was ligated and fixed, and the skin and subcutaneous layers were sutured layer by layer, placed in a 35 • C incubator to wake up, and then put back into the cage.

Infrared Thermal Imaging and Photothermal Therapy in vivo
Two weeks after the surgery, ApoE-/-mice were divided into two groups: control group and experiment group. The mice were locally injected with PBS or Cu 3 BiS 3 nanocrystals. The mice were simultaneously irradiated to the 808 nm lasers (0.4 W/cm 2 , 5 min). An infrared thermal imaging camera was used to detect the temperature change of the mice during the treatment.

Histological Analysis
The overall status of the mice in each group was observed after surgery. The mice were sacrificed by spinal dislocation on 14 days of photothermal treatment. The left carotid artery and main organs of each group were surgically obtained and dehydrated with 10% sucrose for 1.5-2 h. After that, they are transferred to 30% sucrose for soaking overnight, and then embed frozen sections with OCT to make continuous sections with a thickness of 6-8 µm. The slices were dried overnight in a dark and ventilated place. The next day, the slices were loaded into a slice box and sealed and stored in a −20 • refrigerator; or after dehydration, they were embedded into paraffin blocks to make continuous slices with a thickness of 6-8 µm. When necessary, the sections were stained for immunofluorescence or immunohistochemical, observed under a fluorescence microscope.

Carotid Tissue Immunofluorescence Staining
Carotid artery slices were soaked in PBS for three times (5 min each time) to remove OCT embedding agent. The membrane was perforated with 0.1% Triton-X for 10 min, rinsed with PBS for three times. 5% goat serum was blocked at room temperature for 30 min. Diluted anti-mouse CD68 and CD31 antibodies were added, respectively. The slides were removed Frontiers in Bioengineering and Biotechnology | www.frontiersin.org the next day and rinsed with PBS for three times. DAPI (1:500) was added dropwise to stain nuclei for 30 s, rinsed twice with PBS for 5 min each time. After anti-fluorescence quenching, the tablet was sealed, observed and photographed under a fluorescence microscope.

Carotid Artery Hematoxylin-Eosin Staining
The slices of carotid artery and main organ tissues were rinsed with distilled water for three times, 5 min each time. Then the slices were stained with hematoxylin and eosin (HE) dye for 2 min, rinsed with distilled water. The slices were gradually dehydrated with 70, 85, 95, and 100% alcohol in sequence. Finally, The slices were observed under microscope after sealing with neutral gum.

CT Imaging
Cu 3 BiS 3 nanocrystals with varied concentrations (0, 0.5, 1.0, 2.0, 4.0 mg/mL) were placed in PE tubes and then scanned by CT imaging system. CT imaging in vivo was performed using Cu 3 BiS 3 nanocrystals as CT contrast agents. ApoE-/mice with carotid inflammation model were locally injected with Cu 3 BiS 3 nanocrystals dispersed in PBS in surgical site.
Pre-and post-injection, the mice were scanned by the same CT imaging system.

Long-Term Toxicity in vivo
The long-term toxicity in vivo of Cu 3 BiS 3 nanocrystals was evaluated by H&E analysis of major organs and the biodistribution of Cu 3 BiS 3 nanocrystals in main organs. For H&E analysis of major organs (lung, liver, spleen, kidney, and heart), healthy mice were intravenously injected with Cu 3 BiS 3 nanocrystals (10 mg·kg −1 , treatment group) or PBS solution (control group), major organs were collected for H&E analysis. The biodistribution of the Cu 3 BiS 3 nanocrystals was evaluated by intravenous injection with Cu 3 BiS 3 nanocrystals (10 mg·kg −1 ). Major organs were achieved at different time points (i.e., 1, 3, 6, 9 days) for H&E analysis.

RESULTS AND DISCUSSION
Hydrophilic Cu 3 BiS 3 nanocrystals were synthesized by hydrothermal method. In Figure 1a  which was much smaller than that of previously reported Cu 3 BiS 3 nanocrystals (Li et al., 2017), indicating that the synthesized nanocrystals were more suitable for bioapplication. This reduction in particle size may be related to the presence of ethylenediamine. As an ion complexing agent, ethylenediamine can control the release rate of iron ions in the reaction, and the size of nanocrystals can be reduced . We further studied the microstructure of Cu 3 BiS 3 nanocrystals with high-resolution transmission electron microscopy (HRTEM). As shown in Figure 1b, the single crystal plane spacing was 0.307 nm, which corresponded to the crystal plane spacing of the (031) plane of Cu 3 BiS 3 nanocrystals. Additionally, all the X-ray diffraction patterns (XRD, Figure 1c) of Cu 3 BiS 3 nanocrystals can be well indexed as Cu 3 BiS 3 nanocrystals. The lattice parameters were consistent with those on the JCPDS file (No. 16-0713). The perfect match indicated that the synthesized Cu 3 BiS 3 nanocrystals with high crystallinity and high purity. We then measured the optical property. As expected, the Cu 3 BiS 3 nanocrystals showed strong absorption in NIR region centered at 905 nm (Figure 1D), indicating that Cu 3 BiS 3 nanocrystals showed great potential as photothermal agents.
The intense NIR absorption of Cu 3 BiS 3 nanocrystals motivated us to evaluate the photothermal performance of Cu 3 BiS 3 nanocrystals. The aqueous dispersions of nanocrystals with varied concentrations were placed in the PE tubes, and excited by an 808 nm laser. The temperature change was recorded by infrared thermal imager. As shown in Figure 2A, the temperature of aqueous dispersions of nanocrystals increased dramatically under the irradiation of the 808 nm laser, while the temperature of pure water showed little change, indicating that Cu 3 BiS 3 nanocrystals exhibited excellent photothermal effect. As the concentration increased, the elevated temperature increased. Obviously, Cu 3 BiS 3 nanocrystals showed concentrationdependent photothermal effect. Figure 2B provides the direct relationship between the concentration of Cu 3 BiS 3 nanocrystals and the temperature. When the concentration was 80 ppm, the temperature of the aqueous dispersion was increased by 37.2 • C, while the temperature of pure water was increased by less than 2 • C, demonstrating the excellent photothermal performance of Cu 3 BiS 3 nanocrystals. To further evaluate the photothermal performance of Cu 3 BiS 3 nanocrystals, the photothermal conversion efficiency of Cu 3 BiS 3 nanocrystals was measured according to previously reported methods. As shown in Figure 2C, 80 ppm of Cu 3 BiS 3 nanocrystals was excited by the 808 nm laser (0.3 W) until the temperature reached equilibrium and no longer changed. Then the laser was removed away, and the cooling temperature during the cooling process was recorded ( Figure 2D). The time constant can be calculated to be 98.3 s. Thus, the photothermal conversion efficiency of Cu 3 BiS 3 was calculated to be 58.6% which is high enough for photothermal therapy.
In order to detect the cytotoxicity of Cu 3 BiS 3 nanocrystals, we used different concentrations of Cu 3 BiS 3 nanocrystals (0∼320 ppm) to co-culture with macrophages for 12 h, and then tested the cell activity of each group by CCK-8 experiment. The results showed that the concentration was below 160 ppm, Cu 3 BiS 3 nanocrystals had no significant effect on the activity of macrophages (Raw264.7); when the concentration reached 320 ppm, Raw264.7 activity decreased significantly (see Supplementary Figure S2A). Based on the photothermal curves of Cu 3 BiS 3 nanocrystals under the action of near-infrared light, Cu 3 BiS 3 nanocrystals showed a good heating effect at a concentration of 40 ppm; the temperature was increase by 20 • C. We thus chose 40 ppm as the concentration used in subsequent experiments.
Macrophage infiltration is one of the main causes of arterial inflammation and arterial stenosis, so we examined the effect of thermal effects based on Cu 3 BiS 3 nanocrystals on macrophage activity. Cell phagocytosis experiments without using an additional target or selective groups have been studied by many groups (Van Furth et al., 1979;Han et al., 2016;Wang et al., 2019). Nanoparticles were mostly aggregated in lysosomes, and macrophages have much larger amounts of lysosomes than endothelial cells (Van Furth et al., 1979;Han et al., 2016;Wang et al., 2019). In that case, macrophages, as a kind immunocyte, phagocytized a higher proportion of nanoparticles than other cells. As shown in Supplementary Figure S2B, the CCK-8 test found that most cells under the co-action of Cu 3 BiS 3 nanocrystals and 808 nm laser irradiation (0.4 W cm −2 ), and the cell activity was significantly higher than those of cells treated with NIR laser at lower power density. As expected, the cell activity of the control group (no NIR laser irradiation) did not change significantly. Meanwhile, NIR Laser alone had almost no effect on the cell activity (Supplementary Figure S3). In addition, we also stained the living/dead cells of each treatment group to further clarify the status of the cells in each treatment group. As shown in Figures 3a-d, no significant cell death was observed in the control group, while nearly 40% of the cells treated with the 808 nm laser (0.1 W cm −2 ) were killed, 55% of the cells treated with the 808 nm laser with a higher power density of 0.2 W cm −2 were killed, and more than 90% of the cells treated with the 808 nm laser were killed when the laser power density was 0.4 W cm −2 , which was consistent with the CCK-8 results.
After verifying that Cu 3 BiS 3 nanocrystals can effectively kill macrophages in vitro, we further used ApoE-/-mice to make arterial inflammation and stenosis models for photothermal therapy in vivo. ApoE-/-mice were divided into two groups: control group and experiment group. The mice were locally injected with PBS or Cu 3 BiS 3 nanocrystals. The mice were simultaneously excited by the 808 nm lasers (0.4 W/cm 2 , 5 min). An infrared thermal imaging camera was used to detect the surface temperature change of the mice during the treatment. The infrared thermal imager dynamically recorded the local temperature changes of the left neck of the mouse. As shown in Figures 4a,b, the local temperature of the Cu 3 BiS 3 + PTT group can rapidly increase to 46.6 • C within 300 s, while the local temperature of the PBS + PTT group still kept below 35 • C during the whole process, showing an obvious contrast in infrared thermography. Therefore, Cu 3 BiS 3 nanocrystals still showed excellent photothermal effect in vivo driven by the 808 nm laser.
Two weeks after photothermal therapy in vivo, the left carotid artery of each group of mice was removed for immunofluorescence staining. In immunofluorescence, we used CD68 as a marker for macrophages and CD31 as a marker for vascular smooth muscle cells. The results showed that the number of infiltrated CD68 + macrophages in the middle artery wall of the control group (PBS + NIR, Figure 5A) was much higher than that of experiment (Cu 3 BiS 3 + PTT, Figure 5B) group. This indicates that the photothermal therapy based on Cu 3 BiS 3 nanocrystals can effectively inhibit the infiltration of macrophages in the inflammatory arterial wall in a short period of time, which may reduce the adverse results caused by the infiltration of a large number of inflammatory macrophages.
In order to further evaluate the effect of ablation of arterial wall macrophages on reducing the thickness of the arterial wall and inhibiting the progress of arterial stenosis, we tested the thickness of the carotid artery wall of mice by HE staining. The results showed that the thickness of the intima/media in the experimental (CBS + NIR) group was much lower than that in the control (PBS + NIR) group (Figures 6A,B). Effectively inhibit the thickening of the intima/media of the arterial wall, thereby reducing the occurrence of arterial stenosis. In addition, this result was consistent with the arterial wall inflammatory macrophage infiltration results ( Figure 6C). The relative quantity of macrophages in the experimental group was much lower than that in the control group. The arterial intima/media thickness was positively correlated with the amount of arterial wall macrophage infiltration to a certain extent, further indicating that inflammatory macrophage infiltration in the artery, the key role in wall hyperplasia. The most sensitive part in a carotid artery should be the thin layer of endothelial cells, however, in the previous study, it has found macrophages are even more vulnerable than endothelial cells Wang et al., 2019). And because the highest temperature in this photothermal proves is lower than 50 • C (Figure 4b), it would not cause much damage to the carotid artery. According to previous studies, photothermal process can cause some inflammation response, while there is also reports indicate that the laser irradiation can inhibited excessive inflammation, facilitated angiogenesis, as well as improving revascularization Ma et al., 2018). As for the material itself, previous work has been proofed that they have little inflammation response . And nti-CD68 receptor-targeted Fe-doped hollow silica nanoparticles were used as dual-modal US/MRI contrast agent for identifying macrophages of aorta ventralis atherosclerotic plaques in ApoE-/-mice (Ji et al., 2018). To sum up, we can think that photothermal therapy based on Cu 3 BiS 3 nanocrystals can effectively suppress the thickening of the arterial wall by ablating inflammatory macrophages in the arterial wall in the short term, thereby effectively inhibiting the occurrence of arterial stenosis.
In addition to the excellent photothermal effect, Cu 3 BiS 3 nanocrystals also exhibited excellent CT imaging performance because of the high attenuation coefficient of bismuth. Cu 3 BiS 3 nanocrystals with varied concentrations (0, 0.5, 1.0, 2.0, 4.0 mg/mL) were placed in PE tubes and then scanned by CT imaging system. As shown in Figure 7A, CT signal was increased with the increase of the concentration of Cu 3 BiS 3 nanocrystals; the CT signal of Cu 3 BiS 3 nanocrystals with a concentration of 4 mg/mL was higher than that of pure water, indicating the excellent CT imaging performance. In addition, CT values increased linearly with the concentration of Cu 3 BiS 3 nanocrystals. The slope was calculated to be 19.55 HU L/g, higher than some of previously reported CT contrast agents ( Figure 7B). We then studied the CT imaging in vivo using Cu 3 BiS 3 nanocrystals as CT contrast agents. ApoE-/-mice with carotid inflammation model were locally injected with Cu 3 BiS 3 nanocrystals dispersed in PBS in surgical site. Pre-and post-injection, the mice were scanned by a CT imaging system. As shown in Figure 7C, there was a significant difference in CT signal pre-and post-injection of Cu 3 BiS 3 nanocrystals in the artery of ApoE-/-mice. Therefore, Cu 3 BiS 3 nanocrystals can be an efficient CT contrast agent for CT imaging of carotid inflammation.
The long-term toxicity in vivo of Cu 3 BiS 3 nanocrystals was evaluated by H&E analysis of major organs and the biodistribution of Cu 3 BiS 3 nanocrystals in main organs. For H&E analysis of major organs (lung, liver, spleen, kidney, and heart), healthy mice were intravenously injected with Cu 3 BiS 3 nanocrystals (10 mg·kg −1 , treatment group) or PBS solution (control group), major organs were collected for H&E analysis. It was found that the shape and size of the cells both in the treatment group and in the control group almost showed no change (Supplementary Figure S4), indicating the low toxicity in vivo of Cu 3 BiS 3 nanocrystals. The biodistribution of the Cu 3 BiS 3 nanocrystals was evaluated by intravenous injection with Cu 3 BiS 3 nanocrystals (10 mg kg −1 ). Major organs were achieved at different time points (i.e., 1, 3, 6, 9 days). It showed (Supplementary Figure S5) that the Cu 3 BiS 3 nanocrystals mainly accumulate in the kidney and spleen after the intravenous administration. The content in these two organs gradually decreased over time, indicating that Cu 3 BiS 3 nanocrystals were mainly degraded through these two organs.

CONCLUSION
In conclusion, Cu 3 BiS 3 nanocrystals with a size of about 12 nm were successfully designed and prepared by a simple hydrothermal method. Cu 3 BiS 3 nanocrystals showed excellent photothermal effect when driven by an 808 nm laser. The photothermal conversion efficiency of Cu 3 BiS 3 nanocrystals was calculated to be 58.6% due to the strong NIR absorption. Under the irradiation of an 808 nm laser, Cu 3 BiS 3 nanocrystals can efficiently kill the macrophages both in vitro and in vivo, which plays an important role in the development of atherosclerosis, thus can be used as an effective way to inhibit the occurrence of hypertension. In addition, Cu 3 BiS 3 nanocrystals can be used as an efficient CT contrast agent for carotid inflammation. Therefore, Cu 3 BiS 3 nanocrystals show great potential for CT imaging guided photothermal therapy of arterial inflammation.

DATA AVAILABILITY STATEMENT
All datasets generated for this study are included in the article/Supplementary Material.

ETHICS STATEMENT
The animal study was reviewed and approved by the First Affiliated Hospital of Bengbu Medical College.

AUTHOR CONTRIBUTIONS
RL, JZ, and YG designed the project and performed the experimental data analysis. RL, JZ, CY, and ZN carried out the experiment. RL and YG wrote the manuscript. All the authors contributed to discussion of the results.

FUNDING
This study was supported by the Natural Science Research Projects Fund in Colleges and Universities of Anhui Province (KJ2019A0327).