AUTHOR=Zheng Judun , Yue Rui , Yang Ronghua , Wu Qikang , Wu Yunxia , Huang Mingxing , Chen Xu , Lin Weiqiang , Huang Jialin , Chen Xiaodong , Jiang Yideng , Yang Bin , Liao Yuhui 

TITLE=Visualization of Zika Virus Infection via a Light-Initiated Bio-Orthogonal Cycloaddition Labeling Strategy

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.940511

DOI=10.3389/fbioe.2022.940511

ISSN=2296-4185

ABSTRACT=Zika virus (ZIKV) is a re-emerging flavivirus that leads to devastating consequences for fetal development. It is crucial to visualize the pathogenicity activities of ZIKA ranging from infection pathways to immunity processes, but the accurate label-ing of ZIKV remains challenging due to the lack of a reliable labeling technique. Herein, we introduce the photo-activated bio-orthogonal cycloaddition to construct a fluorogenic probe for the labeling and visualizing of ZIKV. Via a simple UV photoirradiation, the fluorogenic probes could be effectively labeled on the ZIKV virus. We demonstrated that it can be used for investigating the interaction between ZIKV virus and diverse cells, and avoiding the autofluorescence phenomenon in traditional immunofluorescence assay. Thus, this biorthogonal-enabled labeling strategy can serve as a promising approach to monitor and understand the interaction between the ZIKV virus and host cell.