Diacylglycerol Kinases in T Cell Tolerance and Effector Function

Diacylglycerol kinases (DGKs) are a family of enzymes that regulate the relative levels of diacylglycerol (DAG) and phosphatidic acid (PA) in cells by phosphorylating DAG to produce PA. Both DAG and PA are important second messengers cascading T cell receptor (TCR) signal by recruiting multiple effector molecules, such as RasGRP1, PKCθ, and mTOR. Studies have revealed important physiological functions of DGKs in the regulation of receptor signaling and the development and activation of immune cells. In this review, we will focus on recent progresses in our understanding of two DGK isoforms, α and ζ, in CD8 T effector and memory cell differentiation, regulatory T cell development and function, and invariant NKT cell development and effector lineage differentiation.


INTRODUCTION
Diacylglycerol (DAG) and phosphatidic acid (PA) are two key lipid second messengers that facilitate efficient receptor-mediated signaling in immune cells along with many other cells. They regulate numerous intracellular signaling molecules to control cell differentiation, proliferation, survival, and function. Following T cell receptor (TCR) engagement, DAG is produced through the activation of Phospholipase Cγ1 (PLCγ1), which hydrolyzes membrane phosphatidylinositol bisphosphate (PIP 2 ) to DAG and inositol trisphosphate (IP3). DAG, together with other signal events, recruits downstream effector molecules to the membrane through their C1 domains and allosterically activates these effectors, with protein kinase Cθ (PKCθ), Ras guanyl-releasing protein 1 (RasGRP1), protein kinase D (PKD), Munc13s, and chimaerins being important for T cell development and/or function (Krishna and Zhong, 2013a;Merida et al., 2015).
PKDs are recruited by both DAG and DAG-activated PKCs. Upon stimulation, inactive PKDs translocate from the cytosol to the plasma membrane in response to membrane DAG production, where they are then activated by novel PKCs (Rozengurt et al., 2005;Spitaler et al., 2006). PKDs have been shown to exert different effects on VDJ recombination at the TCRβ locus and on CD4 and CD8 expression during T cell development based on their localization at the cytosol or plasma membrane (Marklund et al., 2003;Spitaler et al., 2006). Additionally, PKD2 acts as a sensitive digital amplifier of TCR engagement, enabling CD8 T cells to match the production of inflammatory cytokines to the quality and quantity of TCR ligands (Navarro et al., 2014).
Chimaerins possess Rac-specific GTPase Activating Protein (GAP) activity (Caloca et al., 1999;Yang and Kazanietz, 2007). Chimaerin isoforms α2 and β2 are expressed at different levels in T cells and have been shown to translocate to the immune synapse and to both participate in TCR signaling and receive regulation from it (Caloca et al., 2008;Siliceo and Merida, 2009). Chimaerins have been found to inhibit TCR-mediated NFAT activation and DAG-dependent actin polymerization to regulate T cell adhesion and chemotaxis (Siliceo et al., 2006).
Phosphatidic acid (PA) is produced both by the activity of DAG kinases (DGKs) and by the phospholipase D (PLD) family of enzymes in T cells. DGKs phosphorylate DAG to convert it to PA, while PLDs mediate the hydrolysis of phosphatidylcholine (Jenkins and Frohman, 2005;Zhong et al., 2008). The removal of PA is mediated by lipins, which can turn off PA-mediated signaling through dephosphorylation, and they have been shown to regulate mast cell function in the immune system (Csaki and Reue, 2010;Shin et al., 2013b). Intracellular levels of PA change dynamically in response to environmental stimuli (Wang et al., 2006). The downstream effector molecules of PA include a multitude of kinases, such as mTOR (Chen and Fang, 2002), phosphatidylinositol-4-phosphate 5-kinase (PIP5K) (Galandrini et al., 2005;Jarquin-Pardo et al., 2007;Micucci et al., 2008;Cockcroft, 2009;Yoon et al., 2011), spingosine kinase (SPHK ½), RAF1 (Ghosh et al., 1996;Shome et al., 1997;Rizzo et al., 1999Rizzo et al., , 2000Andresen et al., 2002), and other molecules, such as Src homology region 2 domain-containing phosphatase 1 (SHP1) (Frank et al., 1999), kinase suppressor of Ras 1 (KSR1, a scaffolding protein that interacts with several components of the Raf-MEK-ERK cascade) (Morrison, 2001;Kraft et al., 2008), and Sos, another guanine nucleotide exchange factor for Ras activation (Zhao et al., 2007). Both PLD and DGK-derived PA has been shown to directly activate mTOR in non-T cells (Chen and Fang, 2002;Avila-Flores et al., 2005). In these cells, PA can also activate mTOR indirectly via ERK (Winter et al., 2010), but such a mechanism has not been examined in T cells. In T cells, DGKα and ζ mainly inhibit TCR-induced mTOR signaling by negative control of DAG-mediated RasGRP1 and likely PKCθ activation Hamilton et al., 2014). However, DGK-derived PA has been shown to promote T cell maturation in the thymus  and to regulate innate immune responses (Liu et al., 2007). Future studies should determine the direct downstream of the effector(s) of PA that mediate its functions in these immune cells.
The diverse and important functions of DAG-and PA-mediated signaling suggest their levels must be tightly controlled temporally and spatially. DGKs switch from DAGmediated signals to PA-mediated signals to dynamically regulate downstream pathways in response to the engagement of the TCR and many other receptors (Merida et al., 2008;Cai et al., 2009;Zhong et al., 2011). In mammals, there are ten DGK isoforms encoded by different genes, some of which also contain splicing variants, adding complexity to this family of enzymes. All DGKs contain a kinase domain and at least two cysteine-rich C1 domains but differ in the homology of their other structural domains as well as their interaction with other biomolecules. Based on their structural distinction and homology, DGKs are classified into five types that may differ in subcellular localization, function, and regulation. The existence of multiple isoforms poses a significant challenge in studying the physiological roles of any specific isoforms in cellular development and functions due to functional redundancies, a fact demonstrated in conventional αβ T cell and iNKT cell development in mice deficient in both DGKα and DGKζ Shen et al., 2011b). Of these ten isoforms, DGKα and DGKζ as well as DGKδ are the major isoforms expressed in T cells (Zhong et al., 2002;Olenchock et al., 2006a;Sakane et al., 2007). Both DGKα and ζ have been found to regulate multiple signaling pathways downstream from the TCR (Zhong et al., 2002(Zhong et al., , 2003Sanjuan et al., 2003;Baldanzi et al., 2011;Gharbi et al., 2011;Gorentla et al., 2011), such as the RasGRP1-Ras-Erk1/2 pathway, the PKCθ-IKK-NFκB pathway, mTOR signaling , and MAP kinaseinteracting serine/threonine kinase (Mnk) 1 and 2 signaling (Gorentla et al., 2013). They control T cell development (Outram et al., 2002;Guo et al., 2008;Almena et al., 2013), activation and anergy (Zhong et al., 2003;Olenchock et al., 2006a;Zha et al., 2006;Baldanzi et al., 2011), survival (Baldanzi et al., 2011Ruffo et al., 2016), secretion (Alonso et al., 2007(Alonso et al., , 2011Chauveau et al., 2014), and effector function Yang et al., 2016b). Besides T cells, DGKζ also regulates the development, survival, and function of mast cells (Olenchock et al., 2006b), B cells (Wheeler et al., 2013), dendritic cells and macrophages (Liu et al., 2007), osteoclasts (Zamani et al., 2015), and NK cells (Yang et al., 2016a). Extensive reviews about DGKs in immune cells have been published recently (Merida et al., 2008(Merida et al., , 2015Zhong et al., 2008;Krishna and Zhong, 2013b). Here, we will focus on recent literature concerning DGKs in T cell tolerance, iNKT cell development and function, and CD8 T cell-mediated antimicrobial and antitumor immunity.

DGKα AND DGKζ IN T CELL TOLERANCE
Clonal deletion of highly self-reactive T cells in the thymic medulla, generation of properly functioning regulatory T cells (Treg), and T cell anergy are among the most important mechanisms of T cell tolerance that prevent autoimmune diseases (Metzger and Anderson, 2011;Xing and Hogquist, 2012). Although DGKα and ζ synergistically promote T cell maturation from the CD4 + CD8 + double positive (DP) to the CD4 + CD8 − or CD4 − CD8 + single positive (SP) stage, no direct evidence has implicated DGKα and ζ in interference with negative selection in establishing central tolerance .
Regulatory T cells generated in the thymus (tTregs) dominantly suppress T cells and other immune cells to prevent autoimmune diseases. However, they also negatively regulate antitumor and antipathogen immune responses. tTregs are derived from CD4 SP thymocytes in the thymic medulla after relatively strong but transient TCR-MHC/peptide engagement and signaling (Mahmud et al., 2014;Li and Rudensky, 2016). They express Foxp3, a key transcription factor that is critical for their development, maintenance, and function. TCR signaling is not only essential for tTreg generation but also required for tTreg homeostasis and function (Kim et al., 2009;Delpoux et al., 2014;Levine et al., 2014;Vahl et al., 2014). Multiple DAGmediated signaling pathways are involved in tTreg development and function, indicated by the impaired tTreg development and function in mice deficient in either RasGRP1-Ras or PKCθ-IKK-NFκB signaling. Both NFκB and AP1 are involved in transcriptional activation of Foxp3 expression and possibly in regulating other tTreg properties (Schmidt-Supprian et al., 2004;Willoughby et al., 2007;Chen et al., 2008;Gupta et al., 2008;Barnes et al., 2009;Medoff et al., 2009). Both the percentage and number of tTregs in the CD4 + population are increased in DGKζ-deficient (but not DGKα-deficient) thymocytes and splenocytes, compared to wild-type (WT) controls (Table 1). Additionally, Foxp3 − CD25 + cells within the CD4 SP thymocytes are increased in a DGKζ-deficient thymus, suggesting that DGKζ negatively controls early tTreg development. The inhibitory effect of DGKζ on tTreg development is found to be dependent on its negative control of the NFκB/c-Rel and RasGRP1-Ras-Erk pathways Schmidt et al., 2013). Of note are reports that DGKα and ζ manifest differential effects on TNFαinduced NFκB activation in tumor cells and fibroblasts, with DGKα positively regulating PKCζ-mediated p65/RelA at serine 311 residue (Yanagisawa et al., 2007;Kai et al., 2009), while DGKζ inhibits TNFα-induced NFκB activation via decreasing NFkB phosphorylation at Ser468/536, its nuclear localization, and its association with CBP (Tsuchiya et al., 2015). It would be interesting to investigate whether such mechanisms also operate in T cells or downstream of TCR to contribute to DGKα and ζ function in tTreg differentiation. It also remains unclear if DGKα and ζ act redundantly or synergistically to control Treg differentiation and function.
T cell anergy is a form of peripheral tolerance whereby T cells that recognize self-antigens in the absence of costimulatory signals are rendered functionally inactive (Schwartz, 2003;Powell, 2006;Fathman and Lineberry, 2007;Chappert and Schwartz, 2010;Kalekar et al., 2016). In anergic T cells, DAGmediated signaling, including Ras/Erk1/2, NFκB, and mTOR activation, is diminished, while Ca ++ -mediated signaling and NFAT are selectively elevated or unhindered (Powell, 2006;Chappert and Schwartz, 2010;Xie et al., 2012; Figure 1). Both DGKα and ζ are expressed at higher levels in anergic T cells than in activated T cells (Macian et al., 2002;Olenchock et al., 2006a;Zha et al., 2006). Deficiency of either DGKα or ζ or inhibition of DGK activity contributes T cell resistance to anergic induction (Olenchock et al., 2006a;Zha et al., 2006), while overexpression of DGKα promotes T cell anergy (Zha et al., 2006). Because DAG and IP3 are produced at an equimolar ratio by PLCγ1 from PIP2, the elevated DGKα and ζ expression in anergic T cells may shift the equilibrium of IP3 and DAG toward the predominance of IP3-Ca ++ -NFAT signaling over DAG signaling and subsequent AP1 induction. NFAT forms a NFAT/AP1 dimer to promote T cell activation, but it also functions as a monomer to induce transcription of anergypromoting molecules, such as Cbl-b and TRAIL (Macian et al., 2002;Wu et al., 2006). It is postulated that elevated DGK activity may lead to NFAT monomer predominance over NFAT/AP1 dimer for anergy induction (Zhong et al., 2008;Krishna and Zhong, 2013a), although experimental evidence has not yet been presented.
An important issue is how DGKα and ζ expression is regulated. The transcription factor early growth response gene 2 (Egr2) is upregulated in anergic T cells and plays an important role in T cell anergy (Zheng et al., 2012). It binds directly to both Dgka and Dgkz promoters to increase the expression of these genes as well as several other anergy-promoting genes (Zheng et al., 2012(Zheng et al., , 2013. Another transcription factor, Foxo1, also directly promotes Dgka transcription (Martinez-Moreno et al., 2012). Foxo1 function, which is regulated by its subcellular localization between the cytosol and nuclei, is sequestered in the cytosolic compartment following Aktmediated phosphorylation, which prevents it from association with target genes. In naïve or unstimulated T cells, nuclear Foxo1 activates Dgka expression. TCR engagement in the presence of CD28 costimulation induces strong PI3K/Akt activation, which may reduce nuclear Foxo1 and subsequent DGKα expression to ensure full T cell activation and avoidance of anergy (Martinez-Moreno et al., 2012). DGKζ expression has also been found to be regulated by microRNA. Two conserved sequences that match to the miR-34a seed sequence are located in the coding region and 3 ′ untranslated region (3 ′ UTR) of Dgkz. miR-34a expression is greatly upregulated in activated T cells. miR-34a directly represses DGKζ expression through targeting both Dgkz 3 ′ UTR and the coding region to promote T cell activation (Shin et al., 2013a).
Emerging evidence demonstrates that tight regulation of DAG-mediated signaling by DGK activity is critical for the development of iNKT cells. Elevated DGKα activity brought about by expressing a membrane-targeted caDGKα in thymocytes under the control of the proximal Lck promoter caused reduced Erk1/2 activation in thymocytes and a 50% decrease of thymic iNKT cells (Almena et al., 2013). Germline deletion of either DGKα or ζ did not significantly alter iNKT cell numbers in mice. However, simultaneous ablation of both enzymes resulted in a drastic decrease in the number of iNKT cells in the thymus and in peripheral lymphoid organs (Shen et al., 2011b), correlated with prolonged DAG accumulation, elevated Ras-Erk1/2 and PKCθ-IKK signaling, and enhanced activation of both mTORC1 and mTORC2 activities in DP thymocytes Gorentla et al., 2011). In DGKα and ζ double knockout mice, there was a decrease in the number of stage 1 to stage 3 iNKT cells. Stage 0 iNKT cells were not examined. The remaining iNKT cells in these mice were mostly CD44 + NK1.1 − stage 2 cells, suggesting that DGKα and ζ promote both early and terminal iNKT cell maturation (Shen et al., 2011b). Interestingly, expression of constitutive active (CA) IKKβ in developing thymocytes caused a severe reduction in the number of stage 1-3 iNKT cells. Thus, DGKα and ζ double deficiency may cause dysregulation of the PKCθ-IKK-NFκB pathway, leading to early iNKT cell developmental blockage. Different from CA-IKKβ, expression of CA-KRas in thymocytes caused a selective blockage of the transition from stage 2 to 3 of iNKT cells and was associated with decreased T-bet expression (Shen et al., 2011b). Because CA-KRas and DGKα and ζ double deficiency caused elevated mTORC1 signaling  and overactivation of mTORC1 in the absence of TSC1 also resulted in a similar iNKT cell terminal maturation defect (Wu et al., 2014b), DGKα and ζ may synergistically promote iNKT cell terminal maturation at least in part by preventing overactivation of the RasGRP1-Ras-Erk1/2-mTORC1 signaling cascade.
The role of DGKs in iNKT effector functions, however, is less clear. DAG-mediated signaling pathways play important roles in T cell activation, effector lineage differentiation, and tolerance (Chen et al., 2012). They are thus expected to be important in iNKT activation and function. For example, PKCθ is essential for iNKT-mediated liver inflammation (Fang et al., 2012). In germline DGKζ-deficient mice, iNKT17, but not iNKT1 cell number, was selectively decreased. Interestingly, iNKT-17 defects caused by DGKζ deficiency can be corrected in chimeric mice reconstituted with mixed WT and DGKζdeficient bone marrow cells, suggesting that DGKζ controls iNKT-17 differentiation via an extrinsic mechanism . Future investigation should define the type of cells that provide such a DGKζ-regulated extrinsic control of iNKT-17 development. Additionally, mTORC1 deficient iNKT cells are defective in activation and are not able to inflict liver damage (Shin et al., 2014). Overactivation of mTORC1 due to TSC1 deficiency shapes iNKT cell effector lineage fates and contributes to their resistance to anergy and enhanced antitumor immunity (Wu et al., 2014a,b). Given the ability of DGKs in regulating mTOR and PKCθ signaling, future studies should determine if DGKs intrinsically regulate iNKT cell functions and effector lineage differentiation under steady state and in various pathologic conditions.

DGKα AND ζ IN CD8 T CELL-MEDIATED ANTIPATHOGEN IMMUNE RESPONSES
CD8 T cells play important roles in immune responses against pathogens, particularly intracellular pathogens. Upon microbial infection, naïve CD8 T cells are activated after engagement of their TCRs with pathogen-derived peptides presented by antigenpresenting cells. They massively expand and differentiate into cytotoxic T cells that are equipped to kill pathogen-infected target cells and secrete proinflammatory cytokines. A typical antigenspecific CD8 T cell-mediated response includes an expansion phase in which CD8 cells proliferate rapidly and differentiate into effector cells, a contraction phase in which 90-95% of effector CD8 cells die due to apoptosis, and a memory maintenance phase in which the remaining 5-10% of cells are retained as fast-responding memory cells (Williams et al., 2006;Harty and Badovinac, 2008;Zhang and Bevan, 2011). During the expansion phase, effector CD8 T cells differentiate into short-lived effector cells (SLECs, CD127 low KLRG1 hi ) and memory precursor effector cells (MPECs, CD127 hi KLRG1 low ) (Kaech et al., 2003;Sarkar et al., 2008). SLECs produce high levels of cytokines but are prone to death, while MPECs have high potential to differentiate to long-lived memory cells.
Engagement of the TCR on naïve CD8 T cells provides a critical signal that initiates their activation and expansion. TCR signal strength and quality regulate both the magnitude of expansion and the effector fates of CD8 T cells (Zehn et al., 2009;Iborra et al., 2013;Marchingo et al., 2014;Fulton et al., 2015) through the Ras-Erk1/2-AP1 and PKCθ-IKK-NFκB signaling pathways (Sun et al., 2000;Priatel et al., 2002;Zhong et al., 2008;Merida et al., 2015). An initial study found that DGKζdeficient mice mounted an enhanced antiviral immune response following lymphocytic choriomeningitis virus (LCMV) infection. These mice showed enhanced expansion of viral-specific effector CD4 and CD8 T cells that contained higher percentages of IFNγproducing cells 7 days after LCMV infection, which resulted in a quicker clearance of the virus than in WT mice (Zhong et al., 2003). A subsequent study further revealed that DGKα and ζ differentially regulate effector and memory CD8 T cell differentiation. While a deficiency of either DGKα or ζ resulted in enhanced effector CD8 T cell expansion, it slightly decreased memory CD8 T cell formation and response to LCMV infection, which correlated with elevated mTORC1 signaling in these cells .
Although deficiency of either DGKα or ζ enhances antiviral immune responses, DGKα and ζ double deficiency actually caused severe impairment of CD8 T cell-mediated responses to Listeria monocytogenes (LM) infection (Yang et al., 2016b). In an ovalbumin (OVA) specific OT1 TCR transgenic model and newly generated floxed DGKζ conditional-deficient mice where DGKα and ζ activity can be selectively deleted in naïve and memory CD8 T cells, it was found that ablation of both DGKα and ζ, but not of the individual DGKα or ζ isoform, impaired primary CD8 T cell responses (Table 1). At the earliest hours after LM-OVA infection, DGKα and ζ double deficient CD8 T cells expressed decreased levels of chemokine receptors CCR4, CCR5, and CXCR3 and showed impaired migration to the draining lymph nodes (dLNs). Cells that migrated to the dLNs were compromised in their proliferative ability due to not yet defined mechanism(s). In contrast to this in vivo setting, DGKα and ζ double deficient CD8 T cells proliferated more vigorously than WT controls in vitro following antigen stimulation, suggesting that the defect in proliferation was not due to intrinsic defects. It would be interesting to determine if DGKα and ζ are involved in regulating T cell/APC engagement for initiation of T cell activation. As a consequence of impaired expansion of DGKα and ζ double deficient CD8 T cells during primary immune responses, formation of memory cells was severely decreased as well. In addition, DGKα and ζ double deficiency compromised memory CD8 T cell function in homeostasis. Ablation of DGKα and ζ in preformed memory CD8 T cells accelerated the decline of these cells due to increased death and decreased homeostatic proliferative renewal (Yang et al., 2016b).
In DGKα and ζ double deficient CD8 T cells, TCR-induced NFκB nuclear localization was surprisingly diminished, although nuclear NFκB was elevated before stimulation (Yang et al., 2016b). A similar situation was also observed in T cells expressing a constitutive active IKKβ. CD8 T cells expressing a constitutive active IKKβ are defective in expansion in vivo following LM-OVA infection and are impaired in TCR-induced nuclear NFκB translocation (Krishna et al., 2012). It is likely, then, that elevated DAG levels may lead to an increase of basal activation of the PKCθ-IKK-NFκB pathway, which may trigger a negative feedback inhibition for TCR-induced activation of this pathway. Further studies should illustrate the exact negative feedback mechanism caused by DGKα and ζ double deficiency and by overactivation of IKKβ.

DGKα AND ζ REGULATE CD8 T CELL AND CAR-T CELL MEDIATED ANTITUMOR IMMUNITY
A tumor microenvironment suppresses T cell mediated antitumor immunity, rendering tumor-infiltrating T cells hyporesponsive or anergic (Abe and Macian, 2013;Crespo et al., 2013). DGKζ-deficient CD8 T cells contain elevated antitumor immunity. DGKζ-deficient mice subcutaneously injected with the EL-4 thymoma had reduced tumor burdens and increased tumor-specific proliferative CD8 effector T cells compared to WT controls (Riese et al., 2011. Both increased Erk1/2 activation and decreased sensitivity to the suppressive cytokine TGF-β in DGKζ-deficient CD8 T cells may be responsible for stronger activation and antitumor immunity (Arumugam et al., 2015).
Recently, chimeric antigen receptor (CAR) T cells (CAR-T cells) have demonstrated superior activity in tumor control and, in some cases, tumor eradication (Fesnak et al., 2016). However, CAR-T cells have manifested limited efficacy for solid tumors in that they are subjected to suppression by the local tumor environment and may become hyporesponsive or anergic. Such hyporesponsive or anergic tumor-infiltrating T cells or CAR-T cells show decreased Ras/Erk activation but elevated DGKα and ζ levels (Moon et al., 2014). Both type 1 and type 2 DGK inhibitors are capable of reversing such hyporesponsiveness in tumor-infiltrating CAR-T cells ex vivo, leading to increased cytotoxicity (Moon et al., 2014). Consistent with this finding, genetic ablation of DGKα, ζ, or both DGKα and ζ enhanced CD8 T cells transduced with a mesoCAR, a CAR with high affinity to the human tumor antigen mesothelin. DGKα and ζ single or double deficient mesoCAR-T cells produced elevated IFNγ production and demonstrated stronger antitumor cytotoxicity than WT controls, which correlated with reduced sensitivity to TGFβ and increased expression of FasL and TRAIL, ligands for the death receptors FAS and TRAIL-RI/RII. Importantly, DGKdeficient mesoCAR-T cells controlled mesothelioma in vivo better than WT controls . The enhancement of CAR-T function by DGKα and ζ double deficiency sharply contrasts with the defective anti-LM responses of DGKα and ζ double deficient CD8 T cells, suggesting differential requirements of DAG-mediated signaling downstream of CARs and TCR and for CAR-T and conventional CD8 T cell activation.

SUMMARY
Over the past few years, our understanding of the DGK family of enzymes in immune cells has been significantly advanced. DGKα and ζ act individually to negatively control T cell activation, effector CD8 T cell differentiation and function during antimicrobial and antitumor immune responses, and tTreg generation. DGKα and ζ also manifest functional redundancy in promoting conventional αβ T cell and iNKT cell development and in enhancing CAR-T cell function. The unexpected severe impairment of CD8 T cell-mediated immune responses to microbial infection in the absence of both DGKα and ζ underscores the importance of fine-tuning DAG levels and also suggests potential negative feedback mechanisms triggered by deregulated DAG-mediated signaling. Defining such mechanisms should shed additional light on the regulation of DAG-mediated signaling pathways. Additional efforts are also needed to illustrate the underlying mechanisms of differential effects of DGKα and ζ double deficiency on CD8 T cells during antitumor and antipathogen immune responses. While DGKα and ζ perform similar or redundant functions, a more prominent role of DGKζ than DGKα in certain aspects of T cell biology, such as effector CD8 T cell differentiation and Treg, development has been noted ( Table 1); however, determinants of such differences between DGKα and ζ remain unclear. The drastic differences observed between DGKα and ζ double and single deficient CD8 T cells during immune responses beg for development of DGK isoform-specific inhibitors. Such inhibitors used individually or in combination may provide great advantages over pan-DGK inhibitors in modulating immune responses for therapeutic purposes in different disease settings to minimize undesirable side effects. Key elements, such as transcription factors, microRNAs, and posttranslational modifications that control the dynamic individual and synergistic functions of DGK isoforms in T cells are beginning to be appreciated and require further exploration for better understanding of their physiological importance and the development of novel strategies enabling selective modulation of DGK α and ζ expression and activities for treating autoimmune diseases, viral infections, and cancer.

AUTHOR CONTRIBUTIONS
SC, ZH, and X-PZ are involved in preparation of the manuscript.

ACKNOWLEDGMENTS
This work is supported by the National Institutes of Health (R01AI079088 and R01AI101206) for X-PZ.