AUTHOR=Qiu Jin , Zhang Zhiyin , Wang Sainan , Chen Yanru , Liu Caizhi , Xu Sainan , Wang Dongmei , Su Junlei , Ni Mengshan , Yu Jian , Cui Xiangdi , Ma Lu , Hu Tianhui , Hu Yepeng , Gu Xuejiang , Ma Xinran , Wang Jiqiu , Xu Lingyan TITLE=Transferrin Receptor Functionally Marks Thermogenic Adipocytes JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=8 YEAR=2020 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.572459 DOI=10.3389/fcell.2020.572459 ISSN=2296-634X ABSTRACT=Background

Thermogenic adipocytes, including beige and brown adipocytes, are critical for thermogenesis and energy homeostasis. Identification of functional cell surface markers of thermogenic adipocytes is of significance for potential application in biological and clinical practices.

Methods

With a combination of RNA-sequencing of in vivo and in vitro models, we identified transferrin receptor (Tfr1), a receptor specialized for cellular iron uptake, as a previously unappreciated cell surface molecule for thermogenic adipocytes compared to white adipocytes. The alternation of Tfr1 levels under physiological and pathological stimuli was assessed, and the mitochondria functionality, browning capacity, and iron metabolism of mature adipocytes were examined with Tfr1 knockdown.

Results

Tfr1 was expressed predominantly in thermogenic adipocytes versus white adipocyte, and its expression levels were tightly correlated with the activation or inhibition status of thermogenic adipocytes under external stimuli. Besides, Tfr1 gene deficiency in thermogenic adipocytes led to reduced thermogenic gene programs and mitochondrial integrity.

Conclusion

Tfr1 functionally marks thermogenic adipocytes and could serve as a potential thermogenic adipocyte surface marker.