lncRNA Gm16410 Mediates PM2.5-Induced Macrophage Activation via PI3K/AKT Pathway

PM2.5 refers to atmospheric particulate matters with a diameter of less than 2.5 μm. The deposit of PM2.5 in lung cells can cause oxidative stress, leading to changes in macrophage polarity, which can subsequently cause pulmonary inflammation. Long-chain non-coding RNA (lncRNA) is a class of transcripts that regulate biological processes through multiple mechanisms. However, the role of lncRNA in PM2.5-induced lung inflammation has not been established. In this study, the biological effects and associated mechanism of lncRNA in PM2.5-induced change in macrophage polarity were investigated. The lncRNA-mediated PM2.5-induced macrophage inflammation and lung inflammation-associated injury were also determined. Mice were exposed to chronic levels of PM2.5, and changes in the expression of lncRNA in the lung were measured by lncRNA microarray. lncRNAs that showed significant changes in expression in response to PM2.5 were identified. lncRNA showing the biggest change was subjected to further analysis to determine its functional roles and mechanisms with respect to macrophage activation. The result showed that a significant reduction in expression of one lncRNA, identified as lncGm16410, was observed in the lung of mice and RAW264.7 cells following exposure to PM2.5. lncGm16410 suppressed PM2.5-induced macrophage activation via the SRC protein-mediated PI3K/AKT signaling pathway. PM2.5 promoted lung inflammation by downregulating the expression of lncGm16410, enhancing the activation of macrophages. Thus, lncGm16410 might provide new insight into the prevention of PM2.5 injury.


INTRODUCTION
Pollution of the environment by fine particulate matter (PM 2 . 5 , aerodynamic diameter ≤ 2.5 µm) has become one of the major issues to threaten public health (Gao et al., 2019). PM 2 . 5 contains mainly polycyclic aromatic hydrocarbons, transition metals, and endotoxin, all of which are known toxic chemicals (Deng et al., 2013;Ming et al., 2017). These particles can easily translocate from the alveoli to the bloodstream to affect the cardiovascular system, so long-term exposure to high concentrations of PM 2 . 5 increases the risk of cardiovascular and respiratory illnesses Kirrane et al., 2019). In recent years, much progress has been made in the prevention and control of PM 2 . 5 , but PM 2 . 5 remains a potential risk factor (Xie and Li, 2019). Despite the plethora of studies that have been conducted to investigate the toxicity of PM 2 . 5 , the exact mechanism of PM 2 . 5 -induced injury is still difficult to ascertain.
Long non-coding RNAs (lncRNAs) are a set of non-protein coding RNAs with a minimum length of 200 bases. lncRNAs modulate biological processes that are crucial for the growth and differentiation of cells as well as for the development of tumors (Ren et al., 2018). Recently, several studies have found that lncRNAs participate in the metastasis of tumor cells by competitively regulating miRNAs (Lin C. et al., 2018). For example, lncRNA AFAP1-AS1 was found to be the antisense DNA strand of the AFAP1 encoding gene locus, which acts as an adaptor protein that regulates actin integrity and links members of the SRC family proteins and other signaling proteins to actin filaments (Wu et al., 2013). These findings support the hypothesis that lncRNAs play an important role in various biological processes in cells injured by PM 2 . 5 .
The main effects of PM 2 . 5 exposure on cardiopulmonary diseases are inflammatory response and oxidative stress, probably due to the stimulation of macrophages by PM 2 . 5 particles deposited in the alveoli and the release of some oxidants and cytokines. Several studies have demonstrated that the PI3K/AKT signaling pathway plays an important role in the activation of macrophage . Therefore, it has become important to investigate the role of lncRNAs in the process of cardiopulmonary disease caused by macrophage activation and the effect of lncRNAs on PM 2 . 5 -regulated macrophage inflammatory response.
Most previous studies on PM 2 . 5 used tracheal instillation, an approach that is different from and that does not simulate the real situation of human exposure. In this study, a dynamic poisoning cabinet was used to construct a mice model, to determine changes in the expression of lncRNAs in mice in response to chronic PM 2 . 5 exposure and establish the role of lncRNA in PM 2 . 5 -induced lung injury and inflammation. The findings suggested that lncRNA could reduce the extent of PM 2 . 5 -induced lung inflammation and suppress PM 2 . 5 -induced activation of macrophage.
Extraction and Composition of PM 2 . 5 PM 2 . 5 was collected from a large-capacity air sampler of Langfang (Hebei, China) from October 2015 to March 2016. The collection of PM 2 . 5 was performed as described previously . Refer to Supplementary Material 1 for the component analysis of PM 2 . 5 .

Animal Experiments and Experiment Design
All animal experiments were approved by the Animal Experimental Committee of Dalian Medical University. Male Balb/c mice (6 weeks old) were obtained from Changsheng Biotechnology Co., Ltd. (Shenyang, China) (Approval No.: 2015-009). The mice were housed in a clean room (23 ± 1 • C and humidity of 45-55%) provided with conventional food and water and maintained under a diurnal cycle of 12-h light and 12-h darkness. To construct the PM 2 . 5 exposure model, 16 mice were divided into two groups with eight animals per group. The first group was kept as healthy control; the environment of the control group and the PM 2 . 5 content in the environment were provided in Supplementary Material 2. The second group was exposed to PM 2 . 5 (PM 2 . 5 group). The PM 2 . 5 exposure was performed using a dynamic poisoning cabinet (HOPE-MED8050, Tianjin Hepu technology co. Ltd) that simulated the atmospheric environment. According to the standard concentration limit of environmental PM 2 . 5 and the ventilation of mice (The body weight in mice is about 30 g, the tidal volume is about 0.1-0.23 ml/kg, and the respiratory frequency is 136-230 times/min, the volume of poisoning warehouse is about 0.3 m 3 ). The exposure concentration of 300 µg/m 3 used in this experiment is about 225 µg/m 3 of human living ambient air environment PM 2 . 5 concentration. The concentration of PM 2 . 5 in the flowing air was 300 µg/m 3 for 4 months exposure, and the mice were exposed for 5 h/day for 5 days/week. At the end of the exposure period, blood was taken from the eyeball, and the lung tissue was removed and stored directly in liquid nitrogen for further experiments.
Cell Culture and CCK8 Assay RAW264.7 and THP-1 cells were cultured in DMEM (Gibco, CA, United States) or 1640 (Gibco) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 µg/ml streptomycin in humidified air at 37 • C with 5% CO 2 . First, RAW264.7 cells were resuspended, then diluted to 5 × 10 4 cells/ml, and then 100 µl of the cell suspension was added to each well of a 96-well plate. These cells were either control cells or those that overexpressed lncGm16410, and they were treated with different concentrations of PM 2 . 5 for a specific time, followed by the addition of 10 µl of Cell Counting Kit-8 (CCK8) reagent and incubation at 37 • C and in the dark for 2 h. After, the OD value of each well was measured by absorbance at 450 nm. Cell survival rates were expressed relative to that of the control.

lncRNA Microarray Analysis
Microarray profiling was carried out by Shanghai Kangcheng Biotechnology Company. Briefly, the lung sample was labeled using Arraystar RNA Flash Labeling Kit, and a hybridization experiment was prepared using the Agilent SureHyb. After the chip was washed, it was scanned by an Agilent DNA Microarray Scanner, and the chip probe signals were acquired using the Agilent Feature Extraction software (version11.0.1.1). The Agilent GeneSpring GX v12.1 software was used to standardize the chip and to select lncRNAs that displayed differential expression.

Plasmid Construction and Transfection
The plasmid that overexpresses lncGm16410 was designed and synthesized by Gene Pharma (Suzhou, China). Refer to Supplementary Material 3 for details.

RNA Isolation and qPCR
The sequences of all primers used and details are listed in Supplementary Material 4.

Western Blotting
Proteins were extracted from the lung tissue as previously described (Zhang et al., 2018a). Refer to Supplementary Material 5 for details.

Enzyme-Linked Immunosorbent Assay (ELISA)
The expression of IL-6 in the serum was detected with an Enzyme-Linked Immunosorbent assay (ELISA) Kit (Boster, Wuhan, China). Blood samples were collected from the eyeballs of the animals and allowed to clot for 30 min at room temperature. The samples were then centrifuged at high speed, and the resulting serum from each sample was removed and stored it at −80 • C.

Flow Cytometry
Cells undergoing apoptosis were counted by flow cytometry using the annexin V-FITC apoptosis kit. The cells were first washed several times with PBS and then resuspended in binding buffer. Annexin V-FITC (5 µl) and propidium iodide (5 µl) were successively added to the cell suspension followed by incubation in the dark and at room temperature. Apoptotic rates were calculated as percentages of cells in the early plus late stages of apoptosis.

Immunofluorescence and Immunohistochemistry
Refer to Supplementary Material 6 for details.

Statistical Analyses
Data were reported as means ± standard error of the means (SEM). Statistical analysis was performed using GraphPad Prism version 7. Student's t-test was used to compare data between two groups whereas a one-way analysis of variance was used to compare data from more than two groups. Statistical significance was considered at the P < 0.05 level.

RESULTS
Differential Expression of lncRNAs in the Lungs of Mice Exposed to PM 2 . 5 To determine whether lncRNAs were involved in the development of lung injury caused by exposure to PM 2 . 5 , a DNA microarray was used to compare the expression profiles of lncRNA in the lungs of mice exposed to PM 2 . 5 with those in the lungs of unexposed mice. Of these lncRNAs that were expressed in the lung of the control group, a total of 201 lncRNAs were upregulated while 106 lncRNAs were downregulated in the lung of the PM 2 . 5 group ( Figure 1A). Among these lncRNAs that showed different expression levels in the PM 2 . 5 group, lncGm16410 was significantly downregulated (Supplementary Figure 1), and the genomic location of the lncGm16410 was shown ( Figure 1B). As our previous study has shown, PM 2 . 5 can induce macrophage exudation and lung tissue inflammation and injury . In the present study, the effect of lncGm16410 on these processes and the possible underlying mechanism was then determined.

Expression of lncGm16410 Was Downregulated in PM 2 . 5 -Exposed Macrophages
Based on accumulating evidence, alveolar macrophages are the effector cells of lung disease . Therefore, RAW264.7 macrophages were used to investigate the injury caused by PM 2 . 5 exposure, and the effect of PM 2 . 5 on lncGm16410 expression was measured in RAW264.7 macrophages. Consistent with the lncGm16410 expression level in the lung tissue, the expression of lncGm16410 in RAW264.7 cells decreased 24 h after exposure to PM 2 . 5 (Figure 2A). Simultaneously, the result showed that after 24 h of LPS treatment (5 µg/ml), the expression of lncGm16410 decreased significantly, which was similar to the results after PM 2.5 exposure (Supplementary Figure 2). Overexpression of lncGm16410 in RAW264.7 cells was used to access whether lncGm16410 expression correlated with PM 2 . 5 -induced changes in macrophage activity. The level of lncGm16410 was significantly upregulated in RAW264.7 cells that overexpressed it ( Figure 2B). In the control group, the survival rate of RAW264.7 cells was significantly reduced after exposure to PM 2 . 5 , but RAW264.7 cells that overexpressed lncGm16410 appeared to exhibit a better survival rate following exposure to PM 2 . 5 ( Figure 2C). Thus, overexpression of lncGm16410 appeared to protect the cells against apoptosis, thereby enhancing viability despite exposure to PM 2 . 5 for 24 h (Figures 2D-F). Taken together, these results indicated that lncGm16410 was involved in the regulation of cell growth when the macrophages were exposed to PM 2 . 5 .
lncGm16410 Regulates Macrophage Activation Under PM 2 . 5 Exposure Recent studies have found that macrophages can be polarized into M1 or M2 subgroups upon activation by environmental stimulators, and the subsequent change in macrophage activity plays a significant role in the pathogenesis of pulmonary inflammation (Becerra-Diaz and Strickland, 2018;Li et al., 2018). First, the CCK8 method was used to determine the effect of PM 2 . 5 on the growth of RAW264.7 and THP-1 cells, and the median lethal dose was used to study the impact of PM 2 . 5 on macrophage activity. The median lethal doses of PM 2 . 5 for RAW264.7 and THP-1 cells were experimentally determined to be 400 and 80 µg/ml, respectively ( Figure 3A). After 12 h of PM 2 . 5 exposure, the macrophage M1 marker protein was slightly upregulated, peaking after 24 h, while the macrophage M2 marker protein was upregulated after 48 h of exposure (Figures 3B,C). However, after 24 h of exposure, the macrophage M1 and M2 marker proteins in THP-1 macrophages were significantly upregulated (Figures 3D,E). Unsurprisingly, the results of qRT-PCR also demonstrated a significant increase in M1-and M2-related markers following PM 2 . 5 exposure ( Figure 3F). Whether lncGm16410 might affect the PM 2 . 5 -induced upregulated levels of M1 and M2 was then investigated. To test this, control RAW264.7 and those that overexpressed lncGm16410 were exposed to PM 2 . 5 , and the changes in macrophage M1 and M2 levels were measured. After 24 h of exposure to PM 2 . 5 , the mRNA and protein levels of M1 and M2 in the cells that overexpressed lncGm16410 were reduced (Figures 3G-I). Compared with the control cells, the increased expression of M1 and M2 proteins in the PM 2 . 5 -treated cells was also detected by an immunofluorescence assay. Immunofluorescence assay also demonstrated that overexpression of lncGm16410 reduced the levels of M1 and M2 proteins in the cytoplasm ( Figure 3J). These results indicated that lncGm16410 affected the phenotypic transformation of macrophages and confirmed that lncGm16410 could participate in the process of PM 2 . 5 -induced macrophage activation.
lncGm16410 Suppresses the Regulation of SRC Expression Under PM 2 . 5 Exposure According to the lncRNA microarray analysis, we found that, of the several genes adjacent to the lncGm16410, such as Taf7l, Timm8a1, and Btk, only Btk was slightly upregulated.
FIGURE 2 | Effect of PM 2 . 5 on the expression of lncGm16410 in RAW264.7 cells. Control RAW264.7 cells and those overexpressing lncGm16410 were subjected to apoptosis assay by different methods. (A) Real-time quantitative PCR analysis of lncGm16410 transcript levels in the cells after being exposed to PM 2 . 5 for different times (n = 3). (B) Real-time quantitative PCR analysis of lncGm16410 that can optimize the transfection efficiency (n = 5). (C) CCK8 assays to determine the survival rate of macrophages exposed to PM 2 . 5 after they were transfected with lncGm16410 (n = 3). (D) Microscopic analysis of the growth status of macrophages exposed to PM 2 . 5 . (E,F) Quantitative analysis of apoptosis (n = 3). Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. *P < 0.5, **P < 0.01, and ****P < 0.0001-levels of significance that are different from the control group.
The result was not statistically significant, which indicates that lncGm16410 may not act as a cis-acting gene. Recent studies have shown that exposure to particulate pollutants in the environment can lead to the activation of SRC proteinmediated signaling pathways, which in turn might trigger autophagy and inflammatory responses (Xu et al., 2016, Xu et al., 2019. To further investigate the changes in SRC protein expression in response to PM 2 . 5 , the expression levels of SRC in RAW264.7 and THP-1 cells were measured following exposure to PM 2 . 5 . Compared with the control cells, the levels of SRC protein in the PM 2 . 5 -exposed cells displayed a rapid and sustained increased level of SRC after PM 2 . 5 treatment (Figures 4A-D), suggesting that PM 2 . 5 could indeed increase the expression of SRC. The effect of lncGm16410 on SRC expression in PM 2 . 5 -exposed cells was then determined. The expression of SRC was attenuated in the cells that overexpressed lncGm16410 (Figures 4E,F). Changes in the expression of SRC were also confirmed by immunostaining in the cytoplasm of cells that overexpressed lncGm16410 ( Figure 4G). lncGm16410 was involved in the regulation of PM 2 . 5 -induced changes in SRC expression.

SRC-Mediated Macrophage Activation via Activating PI3K/AKT Signaling
Although lncGm16410 suppressed SRC expression under PM 2 . 5 exposure, it is unclear whether SRC was involved in PM 2 . 5mediated macrophage activation. Dasatinib is an inhibitor of SRC protein (Hermida-Prado et al., 2019). To determine whether SRC might be involved in PM 2 . 5 -mediated macrophage activation, the cells were treated with dasatinib before they were exposed to PM 2 . 5 (Figures 5A,B), and the changes in macrophage polarity markers were measured. Compared with control cells, cells that were treated with dasatinib exhibited reduced levels of M1 and M2 expression as revealed by western blot (Figures 5C-F). The inhibitory effect of dasatinib on the expression of NOS2 and ARG1 was also confirmed by immunostaining ( Figure 5G). The result indicated that SRC was involved in PM 2 . 5 -induced (J) Representative images of immunocytochemical staining analysis of the expression levels of NOS2 and ARG1 in RAW264.7 cells that overexpressed lncGm16410. Scale bar = 20 µm. All graphical data are the means ± SEMs from at least three independent experiments, each performed in triplicate. *P < 0.5, **P < 0.01, ***P < 0.001, and ****P < 0.0001-levels of significance that are different from the control group. (G) Representative images of the immunocytochemical staining analysis of SRC level in RAW264.7 cells that overexpressed lncGm16410. Scale bar = 20 µm. All graphical data are the means ± SEMs from at least three independent experiments, each performed in triplicate. *P < 0.5, ***P < 0.001, and ****P < 0.0001-levels of significance that are different from the control group. macrophage activation. As an important molecule in the signal transduction pathway, SRC can shuttle between different cellular compartments to affect cellular processes by mediating the PI3K/AKT signaling pathway (Cheng et al., 2014;Cho et al., 2016). Therefore, to further study the mechanism by which SRC regulates macrophage activity upon exposure to PM 2 . 5 , the expression levels of P-PI3K, P-AKT, and nuclear factor kappa B (NF-κB), proteins that are involved in the PI3K/AKT signaling pathway, were measured by western blot. Macrophages that were exposed to PM 2 . 5 displayed reduced expression of P-PI3K, P-AKT, and NF-κB when the cells were treated with dasatinib ( Figures 5H-K), suggesting that SRC might be involved in the activation of the PI3K/AKT signaling pathway through regulating the phosphorylation of PI3K and AKT. According to accumulated evidence, the main consequence of macrophage activation is the secretion of related inflammatory factors (He et al., 2017;Zhang et al., 2018b). Therefore, changes in the levels of IL-6, TNF-α, and IL-1β of macrophages under PM 2 . 5 exposure were measured. Compared with the control  cells, the expression of inflammatory factors in the PM 2 . 5exposed cells was upregulated (Figures 6A-F). PM 2 . 5 exposure led to a slight decrease in the levels of related inflammatory cytokines in RAW264.7 cells that overexpressed lncGm16410 ( Figure 6A). In addition, RAW264.7 cells that overexpressed lncGm16410 treated with PM 2 . 5 also displayed reduced levels of IL-6 and IL-1β (Figures 6C,D). These results indicated that overexpression of lncGm16410 may reduce inflammation by regulating macrophage activity upon exposure to PM 2 . 5 .

Macrophage Activation Caused Inflammation of Lung Tissue in Response to PM 2 . 5
Numerous studies have shown that PM 2 . 5 could induce lung tissue injury by activating macrophages (Feng et al., 2019). To determine the activation of macrophages in lung tissue under PM 2 . 5 exposure, immunofluorescence was applied to detect the macrophage marker F4/80 in the lung. Compared with the control mice, a large number of activated macrophages in the lung of mice exposed to PM 2 . 5 were observed ( Figure 7A). In addition, for mice that were exposed to PM 2 . 5 , histopathological examination of the lung tissue by H&E staining revealed a slight destruction of the alveolar wall and infiltration of inflammatory cells, including macrophages and lymphocytes, with red blood cells scattered in the alveolar areas ( Figure 7B). To further characterize the inflammatory response in vivo, the levels of proteins and genes associated with inflammatory cytokines in the lung tissue were measured. Compared with the control group, the levels of TNF-α and IL-1β were significantly increased in response to PM 2 . 5 exposure (Figures 7C,D). Moreover, exposure to PM 2 . 5 also led to a significant increase of IL-6 levels in the serum ( Figure 7E). The levels of TNF-α, IL-1β, and IL-6 were subsequently measured by qRT-PCR, which revealed obvious increases as a result of PM 2 . 5 exposure ( Figure 7F). Finally, the link between the activation of the PI3K/AKT signaling pathway and PM 2 . 5 -induced injury in the lung was investigated. The levels of PI3K, AKT, and NF-κB and their phosphorylation were upregulated in response to PM 2 . 5 exposure (Figures 7G,H). This finding was confirmed by immunohistochemical assay of P-AKT and NF-κB proteins. A large amount of brown-yellow fine particles was deposited in the section of the lung exposed to PM 2 . 5 ( Figure 7I). Taken together, the results showed that macrophage activation in response to PM 2 . 5 exposure could cause lung tissue injury.

DISCUSSION
In recent years, PM 2 . 5 , a major environmental pollution component, has attracted much attention because of its direct impact on health. Exposure to PM 2 . 5 can result in a variety of lung diseases and even brain injuries (Huang et al., 2019;Tian et al., 2019). In this study, we collected the PM 2 . 5 sample from the air in Hebei Province, the most polluted city in China, and analyzed its composition. The heavy metals and aromatic hydrocarbons present in PM 2 . 5 are considered to be the main components of PM 2 . 5 toxicity. Next, we used a dynamic poison cabinet to simulate the state of PM 2 . 5 in the environment and used Balb/c mice to construct a PM 2 . 5 exposure model in vivo.
According to related research, the microvesicles released in vitro from LPS-primed alveolar macrophages carry a large amount of TNF-α and a small amount of IL-1β/IL-6, and alveolar macrophages interact with lung epithelial cells through these (I) Immunohistochemistry detection of P-AKT and NF-κB in mouse lung tissues. Scale bar = 10 µm. Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. *P < 0.5 and **P < 0.01-levels of significance that are different from the control group at the levels, respectively. microvesicles (Soni et al., 2016a;Allard et al., 2018). When a PM 2 . 5 suspension is instilled into the trachea of mice, the microvesicles released by macrophages can induce an increase in the number of BALF neutrophils as well as an increase in the expression of related inflammatory proteins (Soni et al., 2016b). Many studies have been carried out to find a way to prevent PM 2 . 5 -induced injuries in macrophages. However, the mechanism and process of these injuries are still unclear.
lncRNAs are widely expressed as non-coding RNA, and increasing evidence suggests that lncRNAs play a major role in human physiological and pathophysiological processes (Chen et al., 2018). lncRNA microarray analysis of lung tissue samples taken from PM 2 . 5 -treated Balb/c mice revealed 201 upregulated and 106 downregulated lncRNAs in response to PM 2 . 5 exposure ( Figure 1A). The same profile of lncRNA expression was observed in RAW264.7 macrophages following exposure to PM 2 . 5 (Figure 2A). This indicated that lncGm16410 may be involved in PM 2 . 5 -induced injuries, both in the lung and in the macrophage. Indeed, the data obtained from the macrophage CCK8 and apoptosis experiments in vitro (Figure 2C), as well as microscopic analysis of the lung tissue, suggested that lncGm16410 might be involved in the activation of macrophages in response to the PM 2 . 5 -induced injuries sustained by the lung.
Notably, we observed that the downregulation of lncGm16410 expression in macrophages was consistent with lncGm16410 expression in the lung. Our data suggested that the initiation of inflammation induced by PM 2 . 5 recruited monocytes to the lungs where they then differentiated into macrophages, ultimately initiating an inflammatory cascade. According to our data, the recruitment of a large number of macrophages to the lung not only increased the expression of lncGm16410 in the lung, but the PM 2 . 5 -activated alveolar macrophages also caused rapid and sustained inflammation as well as the production of cytokines and chemokines. These cytokines and chemokines released by the macrophages then diffused into the lung, causing tissue damage.
Under the stimulation of environmental factors, macrophages will carry out two different forms of inflammatory and antiinflammatory functions (Saradna et al., 2018). M1 macrophages participate in the classic activation pathway and mainly produce immune-stimulating cytokines and other inflammatory response effectors (Sica et al., 2015). M2 macrophages are mainly involved in the upregulation of ARG1 (Nandakumar et al., 2017;Tardito et al., 2019). They have the immunomodulatory capacity, and they also play a major role in tissue remodeling, tumor progression, and mediating the regression of inflammation (Sica and Mantovani, 2012). In the present study, macrophage M1 phenotype-associated proteins NOS2 and IL-12 were found to be abundantly expressed after 24 h of exposure to PM 2 . 5 , suggesting that PM 2 . 5 might stimulate the macrophage to produce an inflammatory response. PM 2 . 5 also induced a slow but late increase in the levels of macrophage M2 FIGURE 8 | Schematic diagram showing the mechanisms through which lncGm16410 mediates PM 2 . 5 -induced macrophages activation. When macrophage is exposed to PM 2 . 5 , lncGm16410 suppresses the regulation of SRC expression, leading to a subsequent increase in the latter. The interactions among lncGm16410, SRC, and PI3K/AKT will dictate the balance of M1/M2 macrophages. Macrophage apoptosis and activation result in the overproduction of inflammatory factors and their subsequent release into the lung. This process, in turn, causes inflammation in the lung. phenotype-associated proteins ARG1 and IL-10, demonstrating that macrophages were fully activated upon PM 2 . 5 exposure ( Figure 3B) and that both M1 and M2 macrophages were involved in macrophage injury induced by PM 2 . 5 . This study mainly focused on the role of lncGm16410 in macrophage activation and showed that lncGm16410 prevented the changes in M1/M2 phenotypes and a decrease in cell viability induced by PM 2 . 5 , indicating a preventative effect of lncGm16410 on macrophage activation. Furthermore, lncGm16410 was found to be involved in macrophage-mediated inflammation responses, suggesting a key role for lncGm16410 in pneumonia (Figures 6A,C).
A recent study on lncRNAs outlines the important roles of these RNAs in the regulation of SRC expression . In the current study, the expression of SRC in macrophages was upregulated in response to PM 2 . 5 exposure. However, overexpression of lncGm16410 counteracted this effect (Figure 4E). One possible explanation for this could be that lncGm16410 downregulated the expression of SRC in macrophages. After exposure to PM 2 . 5 , inhibition of SRC in macrophages resulted in different degrees of reduction in the expression of macrophage phenotypic proteins (Figures 5C,E). This suggested that SRC could regulate PM 2 . 5induced macrophage activity. Taken together, these results verified that lncGm16410 affects macrophage activation by downregulating the expression of SRC in response to PM 2 . 5 exposure. SRC was recently found to be involved in PI3K/AKT signaling pathway (Lu et al., 2003;Riggins et al., 2003;Yang et al., 2017;Beadnell et al., 2018). Our data showed that activation of the PI3K/AKT signaling pathway was also inhibited when the activity of SRC was reduced by the inhibitor dasatinib (Figures 5H,J).
Although further experiments are needed to clarify the detailed mechanism, the current result does suggest a connection between SRC and PI3K/AKT signaling pathway.
Based on a previous study from our laboratory, acute exposure to PM 2 . 5 can cause lung tissue inflammation and oxidative stress . However, mice exposed to PM 2 . 5 exhibited only a small amount of red blood cells and exudates in the alveoli in their lung tissue ( Figure 7B). One possible explanation for this phenomenon could be that chronic exposure to PM 2 . 5 simulated in this experiment was not a traditional tracheal instillation method but was carried out in the form of dynamic exposure. Although this exposure method did not cause any obvious pathological changes in the lung tissue exposed to PM 2 . 5 , it still resulted in some changes to the levels of inflammationassociated proteins and genes in the lung. According to the results of this study, we found that PM 2.5 exposure can induce the expression of inflammation-related factors in the murine-and human-derived macrophages (RAW264.7 cells and THP-1 cells) and mouse lung tissue, which may cause lung tissue injury and then lead to lung inflammation in mice. As this study adopted a dynamic PM 2.5 exposure method, mice were exposed to PM 2.5 through inhalation for 4 months, which can better simulate the long-term exposure of humans to PM 2.5 in the environment. Simultaneously, by comparing relevant epidemiological studies (Zhao et al., 2017;Lin H. et al., 2018;Qi et al., 2020) this study found that PM 2.5 exposure in the environment increases the risk of lung disease and affects the quality of life and life expectancy.
In in vitro studies, the macrophages were transfected with a plasmid that overexpressed lncGm16410. The results were consistent with the decrease in inflammatory cytokines observed in the lncGm16410-overexpressing cells (Figures 6A,C). The reduced mRNA and protein levels of inflammatory factors such as TNF-α shown by lncGm16410-overexpressing cells indicated that the lncGm16410 may suppress the level of inflammatory responses in the lung by reducing the expression of TNF-α in macrophages ( Figure 7C). These results firmly demonstrated the involvement of lncGm16410 in macrophage activation and macrophage inflammation via PI3K/AKT.
Our analyses of PM 2 . 5 -exposed mice suggested that a decrease in lncGm16410 expression coincides with macrophage apoptosis and activation (Figure 8). Thus, both in vivo and in vitro results supported the speculation that lncGm16410 may serve as a potential marker of pneumonia caused by PM 2 . 5 and may be considered a new therapeutic strategy for lung inflammation.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

ETHICS STATEMENT
The animal study was reviewed and approved by all animal experiments were approved by the Animal Experimental Committee of Dalian Medical University.