AUTHOR=Vicente Manuel M. , Chaves-Ferreira Miguel , Jorge João M. P. , Proença João T. , Barreto Vasco M. TITLE=The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=9 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.718466 DOI=10.3389/fcell.2021.718466 ISSN=2296-634X ABSTRACT=

The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.