AUTHOR=Oyama Linda B. , Crochet Jean-Adrien , Edwards Joan E. , Girdwood Susan E. , Cookson Alan R. , Fernandez-Fuentes Narcis , Hilpert Kai , Golyshin Peter N. , Golyshina Olga V. , Privé Florence , Hess Matthias , Mantovani Hilario C. , Creevey Christopher J. , Huws Sharon A. TITLE=Buwchitin: A Ruminal Peptide with Antimicrobial Potential against Enterococcus faecalis JOURNAL=Frontiers in Chemistry VOLUME=5 YEAR=2017 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2017.00051 DOI=10.3389/fchem.2017.00051 ISSN=2296-2646 ABSTRACT=

Antimicrobial peptides (AMPs) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for AMP discovery. We sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against Enterococcus faecalis. Buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. The antimicrobial activity of the recombinant peptide was assessed using a broth microdilution susceptibility assay to determine the peptide's killing kinetics against selected bacterial strains. The killing mechanism of buwchitin was investigated further by monitoring its ability to cause membrane depolarization (diSC3(5) method) and morphological changes in E. faecalis cells. Transmission electron micrographs of buwchitin treated E. faecalis cells showed intact outer membranes with blebbing, but no major damaging effects and cell morphology changes. Buwchitin had negligible cytotoxicity against defibrinated sheep erythrocytes. Although no significant membrane leakage and depolarization was observed, buwchitin at minimum inhibitory concentration (MIC) was bacteriostatic against E. faecalis cells and inhibited growth in vitro by 70% when compared to untreated cells. These findings suggest that buwchitin, a rumen derived peptide, has potential for antimicrobial activity against E. faecalis.