Impact Factor 3.994

Frontiers reaches 6.4 on Journal Impact Factors

Frontiers in Chemistry

Agricultural Biological Chemistry

This article is part of the Research Topic

Drought Tolerance in Crop Plants

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Chem. | doi: 10.3389/fchem.2018.00034

Stress inducible overexpression of AtHDG11 leads to improved drought and salt stress tolerance in peanut (Arachis hypogaea L.)

 Jayanna Naik Banavath1,  Thammineni Chakradhar2,  Panditi Varakumar1, Sravani Konduru1, Krishna Kumar Guduru1,  Chandra Sekhar Akila3, Sudhakar Podha4 and  Chandra Obul Reddy Puli1*
  • 1Department of Botany, Yogi Vemana University, India
  • 2International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), India
  • 3Yogi Vemana University, India
  • 4Biotechnology, Acharya Nagarjuna University, India

Peanut is an important oilseed and food legume cultivated as a rain-fed crop in semi-arid tropics. Drought and high salinity are the major abiotic stresses limiting the peanut productivity in this region. Development of drought and salt tolerant peanut varieties with improved yield potential using biotechnological approach is highly desirable to improve the peanut productivity in marginal geographies. As abiotic stress tolerance and yield represent complex traits, engineering of regulatory genes to produce abiotic stress-resilient transgenic crops appears to be a viable approach. In the present study, we developed transgenic peanut plants expressing an Arabidopsis homeodomain-leucine zipper transcription factor (AtHDG11) under stress inducible rd29Apromoter. A stress-inducible expression of AtHDG11 in three independent homozygous transgenic peanut lines resulted in improved drought and salt tolerance through up-regulation of known stress responsive genes(LEA, HSP70, Cu/Zn SOD, APX, P5CS, NCED1, RRS5, ERF1, NAC4, MIPS, Aquaporin, TIP, ELIP ) in the stress gene network , antioxidative enzymes, free proline along with improved water use efficiency traits such as longer root system, reduced stomatal density, higher chlorophyll content, increased specific leaf area, improved photosynthetic rates and increased intrinsic instantaneous WUE. Transgenic peanut plants displayed high yield compared to non-transgenic plants under both drought and salt stress conditions. Holistically, our study demonstrates the potentiality of stress-induced expression of AtHDG11 to improve the drought, salt tolerance in peanut.

Keywords: peanut, AtHDG11 (Arabidopsis Homeodomain globarous11), Drought stress, High-salinity stress, Water use efficiency, Yield Potential

Received: 31 Aug 2017; Accepted: 12 Feb 2018.

Edited by:

Raju Datla, National Research Council Canada (NRC-CNRC), Canada

Reviewed by:

Giridara Kumar Surabhi, Regional Plant Resource Centre, India
Peng Gao, University of Saskatchewan, Canada  

Copyright: © 2018 Banavath, Chakradhar, Varakumar, Konduru, Guduru, Akila, Podha and Puli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Chandra Obul Reddy Puli, Yogi Vemana University, Department of Botany, Kadapa, 516003, AP, India, pcoreddy@gmail.com