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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Chem. | doi: 10.3389/fchem.2018.00454

Factorial and economic evaluation of an aqueous two-phase partitioning pilot plant for invertase recovery from spent brewery yeast

 Patricia Vázquez-Villegas1, Edith Espitia-Saloma1,  Mario A. Torres-Acosta1,  Federico Ruiz-Ruiz1, Marco Rito-Palomares2 and  Oscar Aguilar1*
  • 1Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Mexico
  • 2Escuela de Medicina y Ciencias de la Salud, Instituto Tecnológico y de Estudios Superiores de Monterrey, Mexico

Aqueous two-phase systems (ATPS) have been reported as an attractive biocompatible extraction system for recovery and purification of biological products. In this work, the implementation, characterization and optimization (operational and economic) of invertase extraction from spent brewery yeast in a semi-automatized pilot plant using ATPS is reported. Gentian violet was used as tracer for the selection of phase composition through phase entrainment minimization. Yeast suspension was chosen as a complex cell matrix model for the recovery of the industrial relevant enzyme invertase. Flow rates of phases did not have an effect, given that a bottom continuous phase is given, while load of sample and number of agitators improved the recovery of the enzyme. The best combination of factors reached a recovery of 129.35 ± 2.76% and a purification factor of 4.98 ± 1.10 in the bottom phase of a PEG-Phosphate system, also resulting in the removal of inhibitor molecules increasing invertase activity as reported by several other authors. Then, an economic analysis was performed to study the production cost of invertase analyzing only the significant parameters for production. Results indicate that the parameters being analyzed only affect the production cost per enzymatic unit, while variations in the cost per batch are not significant. Moreover, only the sample load is significant, which, combined with operational optimization results, gives the same optimal result for operation, maximizing recovery yield (15% of sample load and 1 static mixer). Overall results of these case studies show continuous pilot-scale ATPS as a viable and reproducible extraction/purification system for high added-value biological compounds.

Keywords: Aqueous two-phase systems, Biosolve, invertase, Operational parameters, Pilot Plants, Spent yeast

Received: 29 May 2018; Accepted: 13 Sep 2018.

Edited by:

Brendan M. Leung, Dalhousie University, Canada

Reviewed by:

Maria Graca Rasteiro, University of Coimbra, Portugal
Gaurav Chaudhary, Bundelkhand University, India  

Copyright: © 2018 Vázquez-Villegas, Espitia-Saloma, Torres-Acosta, Ruiz-Ruiz, Rito-Palomares and Aguilar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Oscar Aguilar, Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Monterrey, Mexico, alex.aguilar@itesm.mx