AUTHOR=Jiang Nan , Li Hongyan , Sun Hongzhe 

TITLE=Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells

JOURNAL=Frontiers in Chemistry

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2019.00560

DOI=10.3389/fchem.2019.00560

ISSN=2296-2646

ABSTRACT=<p>Fluorescent probes such as thiol-reactive and Ni<sup>2+</sup>-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His<sub>6</sub>-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed.</p>