Original Research ARTICLE
Peroxynitrite activated drug conjugate systems based on a coumarin scaffold towards the application of theranostics
- 1University of Bath, United Kingdom
- 2University of Texas at Austin, United States
- 3East China University of Science and Technology, China
Two novel drug-conjugates based on a ‘coumarin linker’ have been designed for the synergic release of a therapeutic agent and fluorescent probe for the potential application of theranostics. The drug conjugates; CC-RNS and CI-RNS were designed to be activated by reactive oxygen species or reactive nitrogen species (ROS/RNS). The fluorescence OFF-ON response was triggered by the peroxynitrite-mediated transformation of a boronic acid pinacol ester to a phenol moiety with simultaneous release of the therapeutic agents (Confirmed by HRMS). The limit of detection for peroxynitrite using CC-RNS and CI-RNS was 0.29 µM and 37.2 µM, respectively. Both CC-RNS and CI-RNS demonstrated the ability to visualize peroxynitrite production thus demonstrating the effectiveness of these probes for use as tools to monitor peroxynitrite-mediated drug release in cancer cell lines.
Keywords: theranostic, Coumarin, Chemosensor, fluorescence, Peroxynitrite (ONOO ) －
Received: 28 Aug 2019;
Accepted: 25 Oct 2019.
Copyright: © 2019 Odyniec, Gardiner, Sedgwick, Han, He, Bull and James. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mx. Maria L. Odyniec, University of Bath, Bath, BA2 7AY, South West England, United Kingdom, M.L.Odyniec@bath.ac.uk