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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Chem. | doi: 10.3389/fchem.2019.00787

Chaperone like attributes of biogenic fluorescent gold nanoparticles: potential to alleviate toxicity of intermediate state fibrils against neuroblastoma cells

 Mohammad Owais1*,  Anzar A. Mujeeb1, Khan B. Farheen1, Ansam F. Alshameri1,  Fauzia Jamal1,  Saba Farheen1, Mohammad Kashif2, Anees Ahmad1 and Irfan A. Ghazi3*
  • 1Interdisciplinary biotechnology unit, Aligarh Muslim University, India
  • 2National Botanical Research Institute (CSIR), India
  • 3Department of Biochemistry, School of Life Sciences, University of Hyderabad, India

In general, neurodegenerative disorders have great deal of correlation with the misfolded as well as aggregated forms of protein-based macromolecules. Among various species formed during aggregation process, protein oligomers have been classified as most toxic entities against several types of living cells. A series of chemicals have been developed to inhibit protein aggregation as a measure to regulate neurodegenerative diseases. Recently, various types of nanoparticles have also been reported as potent inhibitors of protein aggregation. In the present study, we synthesised fluorescent gold nanoparticles (B-AuNPs) employing Olax scandens leaf extract. Next, we executed an in vitro study to elucidate the effect of as-synthesized B-AuNPs on the aggregation behaviour of the ovalbumin (OVA), a model protein. We performed extensive study to elucidate anti-amyloidogenic properties of nano-sized entities and established that small sized B-AuNPs manifest chaperone potential against protein aggregation. Further, we exploited as-synthesised B-AuNPs in prevention of protein aggregation mediated toxicity to neuroblastoma cells.

Keywords: gold nanoparticles, Biogenic nanoparticles, Chaperone, Amyloid, Fibril

Received: 24 Aug 2019; Accepted: 01 Nov 2019.

Copyright: © 2019 Owais, Mujeeb, Farheen, Alshameri, Jamal, Farheen, Kashif, Ahmad and Ghazi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mx. Mohammad Owais, Interdisciplinary biotechnology unit, Aligarh Muslim University, Aligarh, 202002, India, mdowais2012@gmail.com
Dr. Irfan A. Ghazi, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Andhra Pradesh, India, drirfang@gmail.com