En Route to a Chiral Melanin: The Dynamic “From-Imprinted-to-Template” Supramolecular Role of Porphyrin Hetero-Aggregates During the Oxidative Polymerization of L-DOPA

Chiral porphyrin hetero-aggregates, produced from meso-tetrakis(4-N-methylpyridyl) porphyrin H2T4 and copper(II) meso-tetrakis(4-sulfonatophenyl)porphyrin CuTPPS by an imprinting effect in the presence of L-3,4-dihydroxyphenylalanine (L-DOPA), are shown herein to serve as templates for the generation of chiral structures during the oxidative conversion of the amino acid to melanin. This remarkable phenomenon is suggested to involve the initial role of L-DOPA and related chiral intermediates like dopachrome as templates for the production of chiral porphyrin aggregates. When the entire chiral pool from DOPA is lost, chiral porphyrin hetero-aggregate would elicit axially chiral oligomer formation from 5,6-dihydroxyindole intermediates in the later stages of melanin synthesis. These results, if corroborated by further studies, may open unprecedented perspectives for efficient strategies of asymmetric melanin synthesis with potential biological and technological applications.


INTRODUCTION
L-3,4-Dihydroxyphenylalanine (L-DOPA; Figure 1A) is an aromatic amino acid produced in various organisms by the oxidative modification of L-tyrosine (Raper, 1927;Mason and Wright, 1949;Haneda and Watanabe, 1971;Prota, 1995;Ito, 2003;Slominski et al., 2012;Marchiosi et al., 2020). In the skin and other organs, like the ink sac of cephalopods, L-DOPA is an early intermediate in the synthesis of black eumelanin pigments from L-tyrosine by the action of the copper enzyme tyrosinase (Slominski et al., 2004;Ito and Wakamatsu, 2007;Simon et al., 2009;Della Vecchia et al., 2013;d'Ischia et al., 2014). In vivo, the process involves the oxidation of tyrosine to dopaquinone followed by intramolecular cyclization to an indoline intermediate, termed leucodopachrome or cyclodopa, which can enter a redox cycle by exchanging electrons with dopaquinone to produce L-DOPA and L-dopachrome. The latter, then, undergoes isomerization with or without decarboxylation and loss of the chiral center to give 5,6-dihydroxyindole (DHI) FIGURE 1 | (A) Schematic illustration of dihydroxyphenylalanine (DOPA) oxidative polymerization to melanin (highlighted is the generation of L-3,4-dihydroxyphenylalanine (L-DOPA) by redox cycling between leucodopachrome and dopaquinone). (B) Molecular structures of porphyrinoids used, 5,10,15,20-tetrakis(4-N-methylpyridyl)porphyrin tetrachloride salt (H 2 T4) and copper(II) 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin tetrasodium salt (CuTPPS). and/or 5,6-dihydroxyindole-2-carboxylic acid (DHICA), respectively (Pezzella et al., 1996;Edge et al., 2006;Ito andWakamatsu, 2007, 2008;Ito et al., 2011;d'Ischia et al., 2013;Panzella et al., 2018). The oxidative polymerization of these latter intermediates leads to the deposition of black insoluble melanin polymers ( Figure 1A). So far, knowledge of the intrinsic chiroptical features of melanin polymers is scant, and little attention has been paid to the possible generation of chiral structures under in vivo or in vitro conditions. The only evidence for the possible occurrence of chirality in melanins is due to the demonstration that oligomers from DHICA may display atropisomerism caused by hindered rotation about interunit bonds (Pezzella et al., 2003). However, current information on the chirality of DHICA oligomers during the polymerization process remains limited.
Recently, a systematic investigation of the effect of porphyrin aggregation on melanin synthesis was undertaken, exploiting the well-known tendency of porphyrins to interact with amino acids (Angelini et al., 2005;Uemori et al., 2012;Charalambidis et al., 2016;Gaeta et al., 2016;Rananaware et al., 2016;Ryu et al., 2018) and polymeric chains (Borovkov et al., 2002a,b;De Luca et al., 2010;Occhiuto et al., 2015;Zhao et al., 2015;Gaeta et al., 2018), with a view to generating new bioinspired functional materials with tailored optical and chiral properties. Water-soluble porphyrins maintain their tendency to aggregate owing to the hydrophobic aromatic macrocycle, whereby binding suitable functional groups to the porphyrin ring may allow to realize self-assembled porphyrin systems in aqueous solution. Although supramolecular arrangements of achiral porphyrins in aqueous solution result in achiral supramolecular structures, chiral aggregates of porphyrins can be formed in the presence of chiral templates (Bellacchio et al., 1998;Onouchi et al., 2006;Toyofuku et al., 2007;Lauceri et al., 2008;Helmich et al., 2010).
Noteworthy, as a consequence of extensive network of interactions (electrostatic, solvophobic, etc.) that trap porphyrin aggregates in a quite deep local energy minimum ensuring kinetic inertia, the porphyrin supramolecular assembly is able to memorize the chiral information imprinted by the chiral template in aqueous solution (Mammana et al., 2007;Gaeta et al., 2016). In this context, porphyrin hetero-aggregates (built by oppositecharged porphyrins) proved to be an ideal system to store chiral information and may offer the possibility of designing switch of memory (Mammana et al., 2007). In this work, we show that porphyrin hetero-aggregates made up of 5,10,15,20tetrakis(4-N-methylpyridyl)porphyrin H 2 T4 ( Figure 1B) and copper(II) 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin CuTPPS ( Figure 1B) can drive the oxidative polymerization of DOPA to melanins with the unexpected generation of asymmetric structures.
The phosphate buffered saline (PBS) tablets were purchased from Sigma-Aldrich Company, and the stock solution was prepared by dissolving one tablet in 200 ml of ultrapure water. PBS buffer (pH = 7.4) contains 10 mM of phosphate buffer sodium salt, 137 mM of sodium chloride, and 2.7 mM of potassium chloride.
L-DOPA and the respective D-enantiomer [D-3,4dihydroxyphenylalanine (D-DOPA)] were purchased from Sigma-Aldrich Company and used without further purification. Solutions of both DOPA enantiomers were freshly prepared by solubilizing the proper amount of solid in PBS buffer in order to attain a final concentration of 0.5 mM.
The porphyrin hetero-aggregates in PBS buffer were obtained by filling with 2 ml of PBS solution a quartz cuvette (path length = 1 cm), then the proper volume of H 2 T4 mother solution was added to reach a 2 µM concentration of H 2 T4 in the sample solution. After 5 min, the amount of CuTPPS was added in order to reach again a 2 µM concentration of CuTPPS in the sample solution. After an additional 20 min, other aliquots of H 2 T4 and CuTPPS were added as illustrated before. The final work solution thus obtained was kept for 20 min before spectroscopic investigations.
The porphyrin hetero-aggregates in the presence of D-and L-DOPA were obtained by using the corresponding DOPA solution (0.5 mM in PBS) following the aforementioned procedure. In detail, the proper volume of H 2 T4 mother solution was added to D-or L-DOPA solution to reach a 2 µM concentration of H 2 T4, then after 5 min, the proper amount of CuTPPS was added to the sample solution in order to reach again a 2 µM concentration of CuTPPS. After an additional 20 min, other aliquots of H 2 T4 and CuTPPS were added as illustrated before. The final work solution thus obtained was kept for 20 min before spectroscopic investigations. For the long incubation time, each solution of DOPA and porphyrin hetero-aggregates was stored in sealed plastic cuvettes in order to limit the adhesion of both porphyrins and DOPA on the cuvette walls.
All solutions, both stock and sample solutions, are prepared in ultraclean conditions: (i) the operators wore a lab coat, hair cap, gloves, and mask during the preparation of samples and (ii) the tips of the pipettes and the plastic cells were washed three times with ultrapure water before being used.
Circular dichroism (CD) and Uv/Vis measurements were carried out at room temperature (quartz cuvette path length 1 cm) on a JASCO J-710 spectropolarimeter and JASCO V-530 spectrophotometer, respectively. Linear dichroism (LD) measurements were carried out on a modified JASCO J-500A spectropolarimeter (Micali et al., 2015) after proper calibration with an oriented polarizer. Linear birefringence of the instrument  optics was also measured in order to evaluate the cross-talk contribution to CD.

RESULTS AND DISCUSSIONS
In PBS buffer (pH = 7.4), the formation of porphyrin heteroaggregates from equimolar amounts of tetra-cationic H 2 T4 and tetra-anionic CuTPPS was apparent by both the hypochromic effect and band broadening in the Soret region, as reported in UV/Vis spectrum (Supplementary Figure 1). In the absence of chiral inducers in solution and under ultraclean conditions, the building of achiral supramolecular structures, as expected, was denoted by zero optical activity in the porphyrin heteroaggregate absorption region (Supplementary Figure 1 inset).
The construction of chiral multicomponent systems requires precise hierarchical rules (Elemans et al., 2003), whereby to prepare chiral porphyrin hetero-aggregates, cationic porphyrin H 2 T4 (4 µM) was to be added to a PBS solution of L-DOPA (0.5 mM) followed by the anionic counterpart CuTPPS (4 µM). UV/Vis spectra confirm the formation of porphyrin heteroaggregate, showing a hypochromic effect and broadening of the Soret bands (Supplementary Figure 2). Noteworthy in the CD spectra, both a positive single Cotton effect of L-DOPA at about 280 nm and an induced bisignate CD signal in visible region, due to chiral exciton coupling of two porphyrin chromophores in hetero-aggregates, were observed (Figure 2). Mirror image was recorded when D-DOPA was used in place of levodopa, confirming that the CD signal in the porphyrin absorption region was induced by the interaction with DOPA via a chirality transfer process (Figure 2).
The solutions containing chiral porphyrin hetero-aggregates and single DOPA enantiomers were incubated for 2 weeks in plastic cuvettes rather than in quartz cuvettes in order to minimize the sticking of DOPA-derived melanin products onto cuvette walls. Although the oxidative polymerization of DOPA evolved slowly in PBS buffer at pH 7.4 as is usually the case with catechol and catecholamine compounds (Bernsmann SCHEME 1 | Chirality transfer dynamics in the oxidative polymerization of L-3,4-dihydroxyphenylalanine (L-DOPA) in the presence of porphyrin hetero-aggregate. et al., 2011), after 2 weeks, significant variations in the DOPA absorption and CD spectra were observed. CD and UV spectra of the sample solutions were recorded after 24 h, 3, 7, and 15 days to follow the evolution of DOPA polymerization. In comparison with the initial situation, main changes observed concerned: i) the loss of the induced CD signal attributed to the porphyrin hetero-aggregates and, simultaneously, ii) a marked change in the dichroic signal of DOPA (Figure 3, Supplementary Figure 3).
Since the chirality transfer mechanism implies a closerange contact between chiral inducer and achiral building blocks (Borovkov et al., 2001;Mammana et al., 2007;Zeng et al., 2009;Randazzo et al., 2019;Ustrnul et al., 2019), the disappearance of the induced chirality may be associated to a de-aggregation of the porphyrin hetero-aggregate owing to the polymerization of DOPA and the associated loss of chirality. In line with this conclusion, the UV/Vis spectra of incubated hetero-aggregates evolved with the growth of the CuTPPS Soret band (λ max = 412 nm) (Supplementary Figure 3), whereas, conversely, no detectable band associated with H 2 T4 (λ max = 423 nm) was observed (Supplementary Figure 3 inset) presumably due to embedment into the developing melanin matrix whose carboxylate residues are deprotonated (thus anionic charged) at pH value of PBS buffer. Indeed, adding acid solution to melanin precipitate (separated from the solution) in order to protonate the carboxylate residues, a band at 450 nm, ascribable to protonated form of H 2 T4, was detected (Supplementary Figure 4). These spectroscopic data suggested that the porphyrin hetero-aggregate in PBS at high ionic strength does not exhibit similar stability as previously demonstrated in water (Mammana et al., 2007;Gaeta et al., 2016). It is likely that ionic strength modulates electrostatic interactions between opposite-charged porphyrins, affecting the stability of the heteroaggregate in PBS.
Remarkably, drastic changes in the CD signals at 450-500 nm are observed with time (1 week timescale; Figure 3), which are paired to a later increase of the signal at 280 nm (2 weeks timescale). Such profile evolution is a clear signature of the generation of asymmetric structures, likely driven by chirally imprinted porphyrin hetero-aggregates during melanin synthesis, while the original chiral information from DOPA was completely consumed because of its conversion into 5,6-dihydroxyindoles. Noteworthy, contributions from LD are negligible; however, the possibility that a component of differential scattering might affect the measurements owing to the presence of melanin particles cannot be ruled out.
To support the above conclusions, the spectroscopic behavior of L-DOPA in PBS buffer was monitored in the absence of porphyrins. The progress of the oxidative polymerization was denoted by the simultaneous decrease of the absorbance (λ max = 280 nm) and related CD signal of L-DOPA (Figure 4,  Supplementary Figure 5). After several days, the final dark solution did not display almost any residual chirality suggesting the formation of achiral melanin.
Further evidences of the role played by porphyrin heteroaggregate as chiral templating agent of melanin oligomers have been gained performing a clear-cut experiment, reversing the order of addition of the components. In detail, we added L-DOPA to a solution of preformed achiral porphyrin hetero-aggregate (Supplementary Figure 6). After 1 week, the CD spectrum of L-DOPA looked similar to the CD spectrum of L-DOPA alone in PBS (Supplementary Figure 6 inset), confirming that chiral porphyrin hetero-aggregate plays a key role in inducing chiral melanin oligomer formation.
To conclude, these results disclose a rare example of temporary chiral mediation in which a chirally imprinted aggregate is decomposed while serving in turn as template for the chiral imprinting of developing oligomer aggregates from nonchiral decomposition products of a chiral precursor (Scheme 1).
These results open a new promising area of investigation on the organization of melanin pigments with applications ranging from biology and medicine to nanotechnology and material science.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

AUTHOR CONTRIBUTIONS
AD'U, Md'I, AP, and RP contributed to the conception and design of the study. Experimental work was carried out by MG, RR, VV, and NM (CD, UV/Vis, LD, differential scattering) under supervision of AD'U. MG wrote the manuscript and prepared images with contributions of AD'U, Md'I, and AP. All authors participated in the analysis and discussion of obtained results.