Determination of Phenolics and Flavonoids of Some Useful Medicinal Plants and Bioassay-Guided Fractionation Substances of Sclerocarya birrea (A. Rich) Hochst Stem (Bark) Extract and Their Efficacy Against Salmonella typhi

Gallic acid and catechin are the most abundant phenolic and flavonoid contents found in all plant extracts. The contents and the bioassay-guided fractionating substances of the Sclerocarya birrea (A. Rich) Hochst (Anacardiaceae) fraction played vital roles. The goals of the study were to determine the contents of some useful medicinal plants and the bioassay-guided fractionation substances of S. birrea fraction compounds capable of acting against Salmonella isolate using LC-MS/LC-HRMS (Dionex ultimate 3000 RS UPLC with Thermo Scientific Q Exactive Orbitrap Hybrid Tandem Mass Spectrometer). The Folin–Ciocalteu reagent procedure and flavonoid content determination were conducted spectrophotometrically. Bioassay-guided fractionation, chronological partitioning, and screening of the antibacterial action against Salmonella typhi were performed. The ethyl acetate fraction extracts of S. birrea stem (bark) extract were analyzed using LC-MS/LC-HRMS. The gallic acid content increased tremendously in Vachellia nilotica (L.) P.J.H. Hurter and Mabb (Fabaceae) pod extracts with curve fitting (R 2 = 0.9958). Catechin content increase was significantly increased in S. birrea stem (bark) extracts followed by that of V. nilotica pod extracts with curve fitting (R 2 = 0.9993); they were all significantly different in the Guiera senegalensis J.F. Gmel. and the Leptadenia lanceolata (Poir.) Goyder leaves extracts at p value <0.0001. Subsequently, 10 mg/ml of S. birrea stem (bark) ethyl acetate fraction extract was the MIC, where no MBC was recorded and susceptible to the positive control with the highest inhibition zone, followed by the ethyl acetate fraction extract at 10 mg/ml (9.7 ± 0.0) at Turkey’s p < 0.0001. Vidarabine is one of the novel compounds, specifically having antimicrobial actions, found in the S. birrea stem (bark). Reasonable amounts of phenolic and flavonoid contents determined the actions of the individual plant extract.


INTRODUCTION
Plant-derived medicines are widely used because they are relatively safer than the orthodox ones. Antibiotic resistance has become a global concern and we are threatened by the emergence of multidrug resistance pathogens (Adoum, 2106). The use of medicinal plant extracts to treat infectious diseases is an age-old practice that relies on traditional medicine (Paudel et al., 2018). Phytochemical ingredients in various parts of the plant vary significantly (Adoum, 2106). The V. nilotica pod extracts have been used in folk medicine (Mustafa et al., 1999). Subsequently, methanolic extracts were revealed to have higher total phenolic contents and good antioxidant activities compared to other extracts. They possessed very good bioactive ingredients for counteracting various ailments (Johari and Khong, 2019).
For G. senegalensis, leaves were taken for many purposes like; pulmonary and respiratory problems, colic and diarrhea, syphilis, beriberi, leprosy, rheumatism, diuresis, impotence and expurgation (Anka et al., 2020). Many bioactive compounds were identified from G. senegalensis, including antioxidants, high contents of polyphenols, tannins, flavonoids, glycosides, and essential oils (Gurama et al., 2020). Subsequently, L. lanceolata has been used locally in treating many ailments, including some physiological imbalances like hypertension, sex ineffectiveness, and milk drying (Malgwi et al., 2019). Phytochemicals have clearly demonstrated a number of different compounds such as alkaloids, flavonoids, phenolic, glycosides, saponins, and tannins in a plant (Mustapa et al., 2018). Phenolics and flavonoids contents of the L. lanceolata were also regarded to be quantified and determined using HPLC as described by (Ja'afar et al., 2013).
Another native African tree is S. birrea, known locally as "Marula." Its stem bark, roots, leaves, and fruits contained many chemical components such as polyphenols, tannins, coumarins, flavonoids, triterpenoids, and phytosterols. It is used as food and traditional medicine for their ailments (Mariod and Abdel). The bioassay-guided fractionation is a technique for plant extract profiling and screening of the bioactive compounds as potential sources of bio-based new drugs. The fractionation procedure is employed to screen natural products in the plant extracts better (Malviya, 2019). The bioassay-guided fractionation of methanolic extracts of Eupatorium triplinerve Vahl (Asteraceae) revealed active antimicrobial components in some solvents, notably hexane, dichloromethane, and ethyl acetate against Escherichia coli at 16 and 31 mg/ml and Pseudomonas aeruginosa at 31 and 62 mg/ml MICs (Regina et al., 2015). Subsequently, the Salmonella paratyphi is a Gram-negative, rod-shaped, facultative anaerobe, non-encapsulated, non-spore-forming, flagellated and motile bacterium. Three serotypes of S. paratyphi were described: S. paratyphi A, B, and C worldwide (Panezai et al., 2018). S. paratyphi transmission was through the fecal-oral route or via eating of unclean food/water and coming in contact with chronic asymptomatic carriers (Panezai et al., 2018). Gastrointestinal ailments are among the major health concerns in most African countries and in other undeveloped countries, which devastated the world by causing high morbidity and fatality rates (Manzo et al., 2017).
Lastly, most of the S. birrea secondary metabolites were also determined using liquid chromatography-mass spectroscopy and NMR. Notably, flavonoids were responsible for fighting various ailments (Mc et al., 2017). The present study is the first to explore selected medicinal plants' contents from Yobe State, Nigeria. Vidarabine, a novel compound, was identified in the S. birrea stem (bark) fraction extract contrary to the earlier studies. The goals of this study were to determine the active compounds of some useful medicinal plants using LC-MS and the bioassayguided fractionating substances from S. birrea stem (bark) fraction extract's potency against bacterial isolates.

Collection of Plant Materials and Their Identifications
The plants voucher specimens collected were processed following a standard herbarium technique (Bridson and Forman, 1999). Subsequently, the samples were identified and authenticated by a plant taxonomist, Dr. Yusuf Nuhu, from the Department of Plant Biology, Bayero University Kano, Nigeria. All the identified plant specimens were given voucher numbers and deposited at the University Herbarium for reference purposes.

Sample Preparation
The plant extracts (crude extracts of the leaf, stem (bark) and pods of Guiera senegalensis J (Combretaceae), Leptadenia lanceolata (Apocynaceae), S. birrea, and V. nilotica) were individually dissolved in methanol and subjected to sonication for 1 h. Subsequently, after the sonication process, the extracts were kept overnight at 4°C. Then, the extracts were centrifuged at 5500 rpm for 10 min (10°C). Finally, the supernatants were used for analyses (Chan et al., 2014).

Total Phenolic Contents
A 0.1 ML of the sample (standard) methanol was reacted with 0.5 ML of 10-fold diluted Folin-Ciocalteu reagent, followed by 0.4 ML of 7.5% (w/v) sodium bicarbonate solution. After incubation at 40°C for 30 min, 200 μg/ML of the reaction mixture was placed into a 96-well plate and the absorbance was recorded at 760 nm spectrophotometrically. Gallic acid was used as the standard (Chan et al., 2014).

Total Flavonoid Contents
A 25 µL of sample (standard) methanol was mixed with 125 µL of ultrapure water and 7.5 µL of 5% (w/v) sodium nitrite solution. After 6 min, 15 µL of 2% (w/v, in ultrapure water) aluminum chloride solution was added and the mixture was allowed to stand for 5min. Then, 50 µL of 1 min sodium hydroxide and 27.5 µL of ultrapure water were serially added to the mixture and the mixture was vortexed for 10 s. Finally, 200 µL of the reaction mixture was placed into a 96-well plate and the absorbance of the mixture was measured at 510 nm (+); catechin was used as the standard (Ho et al., 2014).

Preparation of the Ethanol Extract and the Fractions
A powdered form of S. birrea stem bark methanolic stem (bark) extract (20 g) was immersed in methanol for some hours, subsequently extracted using a standard separating funnel, and the solvent was evaporated under vacuum. The same extract was then suspended in water and partitioned successively with n-hexane, chloroform, and ethyl acetate. Each fraction was then evaporated in a vacuum with their respective yields. The extracts were concentrated under reduced pressure at 50°C and lyophilized to obtain powder for further use (Huang et al., 2011). Moreover, fractions of the solvents used were dissolved in DMSO in mg at variant concentrations for the antibacterial actions to affect their modes of action against the bacterial isolate (Huang et al., 2011;Cunha et al., 2017).

Solvent Partitioning
The solvent partitioning was done through a procedure used by (Jamil et al., 2012), with a few modifications. The crude plant extract was subjected to bioassay-guided fractionation with solubilization in water and a chronological partition with n-hexane (2 × 125 ml), chloroform (2 × 125 ml), and ethyl acetate (5 × 125 ml) as indicated in Figure 1. Then, each of the fractions obtained was evaporated to dryness and later screened by the antibacterial assay (Mc et al., 2017;Cunha et al., 2017).

Testing Isolate Using the Fraction Extracts
Broth culture of the isolates was adjusted to correspond to a McFarland standard (Thermo Scientific) (∼10 6°C fu/ml). The inoculum was streaked using a swab stick onto the Mueller-Hinton agar (HimediaR) and allowed to stand on five prepared paper discs of plant extract concentrations using sterilized forceps. The inoculated Mueller-Hinton agar (HimediaR) plates were incubated at 37°C for 24 h. The plates' zones of inhibition were read and measured to the nearest mm in diameter and recorded in three different trials (Nneka et al., 2016). Furthermore, both the MICs and the MBCs of the respective fraction extracts against the isolate (S. typhi) were tested following Abdullah et al. (2019) and Nneka et al. (2016), with a few modifications.

LC-MS Procedure
The sample was analyzed using ultra-performance liquid chromatography with high-resolution mass spectrometry (LC-MS/LC-HRM) for the identification of compounds. The method used employed reversed-phase chromatography with a gradient range of solvent strengths. The online high-resolution accurate mass (HRAM) fragmentation library contains highly curated MS/ MS and MSn spectra from different collision types and collision energies. Cloud Search was integrated into the compound discoverer along with other tools, such as predicted compositions based on high-resolution full MS and ChemSpider search, that helped partially identify the compounds. Built-in FISH scoring was used to verify hits from ChemSpider against the MS2 data.

Sample Preparation
One mg of extract was diluted in 1 ml methanol as the master stock (MS) and 10 μg/mL as the working stock (WS) in methanol before analysis. All samples were filtered with a 0.22 um PTFE membrane filter and transferred to 2 ml vials for analysis.

Operating Conditions
The results of the (LC-MS/LC-HRMS) were subjected to the Thermo Scientific Compound Discoverer software version 3.1 for online compound database matching using cloud and ChemSpider.

Statistical Analyses
All experiments were carried out in triplicate and the results were expressed as mean values and standard deviations. One-way analysis of variance (ANOVA) was performed using GraphPad Prism version 8.0 and the differences between samples were compared using Turkey's test (p < 0.05).

Quantification of Phenolic Contents of
Sclerocarya birrea, Vachellia nilotica, Guiera senegalensis, and Leptadenia lanceolata Extracts Gallic acid increased tremendously, especially in V. nilotica pods extracts (210.063%) and S. birrea stem (bark) methanolic and ethanolic extracts with curve fitting (R2 0.9958), as shown in Figure 2, showing a very good curve fit across the medicinal plants, and all were significantly different against the G. senegalensis leaf extracts and the L. lanceolata leaves extracts with a very low content at F (15, 32) 6,676, at p value < 0.0001.

Quantification of Flavonoid Contents of
Sclerocarya birrea, Vachellia nilotica, Guiera senegalensis, and Leptadenia lanceolata Extracts Catechin was significantly increased was noticed in S. birrea stem (bark) extracts (38.81%), followed closely by V. nilotica pod extracts (25.6469%) with curve fitting (R 2 0.9993). The S. birrea stem (bark) extracts (38.81%) were followed closely by V. nilotica pod flavonoid contents. The S. birrea stem (bark) methanolic and ethanolic extracts were the highest extracts with more flavonoid contents, followed by V. nilotica ethanolic pod extract and methanolic pod extract, which were also significantly different at p value < 0.0001 across the remaining extracts, which were revealed to have comparatively low contents, especially L. lanceolata leaf extracts.

Bioassay-Guided Fractionating Substances of Sclerocarya birrea (A. Rich) Hochst. Stem (Bark) Extract Against Salmonella typhi
A 2.16 g S. birrea ethanolic stem (bark) crude extract had procured a 10.8% yield. The same extract yielded the following: 0.165 g residue of n-hexane with a fraction percentage yield of 2.05%; 0.0133 g chloroform with a fraction percentage yield of 1.65%; 0.2645 g ethyl acetate with a fraction percentage yield of 32.88%. The final recovered yield was 0.4711 g, with the final % yield recovered being 58.5654, after partitioning as depicted in Figure 1. Moreover, the fractions were subjected to antibacterial actions to affect their modes of action against the isolate. Subsequently, 2.5 mg/ml, 5 mg/ml, and 10 mg/ml happened to be the concentrations with such activities. These were subjected to the MICs, where 10 mg/ml of the S. birrea stem (bark) ethyl acetate fraction extract was the MIC and also there was no MBC on the fraction extract. Therefore, it inhibited the growth of the S. typhi, not killing it. Moreover, the statistical test showed that the isolate was susceptible to the positive control (ciprofloxacin 30.33 ± 0.0) as the highest inhibition zone, followed by the ethyl acetate fraction extract at 10 mg/ml (9.7 ± 0.0), and resistance was very significant to the respective concentrations on the remaining fraction extracts including the chloroform fraction extract at Turkey's p < 0.0001 , as shown in Figure 3. Frontiers in Chemistry | www.frontiersin.org July 2021 | Volume 9 | Article 670530 LC-HRMS identified 16 compounds with different fragmentation ions and various structures as far compounds. Vidarabine appeared to be the best compound with 99.9 MZCloud best full matches, followed by N, N-dimethylaniline, L-phenylalanine, catechin gallate, gallic acid, and meprednisone acetate as the least with partial matches, as shown in Table 1 and their respective structures in Figure 4. They showed the plant to be active against S. typhi. Subsequently, N, N-dimethyl aniline was identified by both MZCloud and ChemSpider (Table 2) Table 1 and Figure 4.
Detailed information regarding the profiled compounds identified using LC-MS/LC-HRMS, with the aid of MZCloud and ChemSpider software, their respective chemical structures, molecular weights, fragmentation rates, and their retention time was extracted from the S. birrea stem (bark) extract as depicted.

DISCUSSION
Medicinal plants were regarded as valuable and most useful natural resources used for the invention of new novel drugs. Many compounds were better known as attributes to the efficacies of the used medicinal plants in treating many ailments caused by microbes (Hajara et al., 2018). The S. birrea fruit is rich in so many minerals and phenolic compounds (tannins, catechin, and hydroxycinnamic acid). Moreover, it is rich in polyphenols, tannins, flavonoids, alkaloids, saponins, coumarins, triterpenoids, phytosterols, and quercetin and also in many flavonoid derivatives. It has been reported that its stem bark shared similar constituents as those of both the leaves and fruits (Ojewole et al., 2009). Nevertheless, the methanolic extracts were revealed to have higher total phenolic contents and good antioxidant activities compared to the other extracts and possessed very good bioactive ingredients to counteract various ailments (Johari and Khong, 2019). In the present study, gallic acid increased tremendously, especially in V. nilotica pods extracts (210.063%) and S. birrea stem (bark) methanolic and ethanolic extracts with curve fitting (R 2 0.9958), as shown in Figure 2. This turned to be very good across the used medicinal plants. There were significant differences against the G. senegalensis leaf extracts and the L. lanceolata leaves extracts with very low content at F (15, 32) 6,676, at p value < 0.0001.
However, large catechin increases were noticed in S. birrea stem (bark) extracts (38.81%) followed closely by V. nilotica pod extracts (25.6469%) with curve fitting (R 2 0.9993), as shown in Figure 5. This happened to be an outstanding curve across the medicinal plants used in the study. The latter also agreed with the fact that V. nilotica pod extracts had been in used as a folk medicine due to their constituents (Mustafa et al., 1999;Johari and Khong, 2019). Similarly, secondary metabolites like tannins, flavonoids, saponins, steroids, terpenoids, and phenols were found to be rich in S. birrea stem bark extracts. Their therapeutic actions had been used to address many ailments in traditional alternative medicine like diarrhea (Manzo et al., 2017). Some phenolic compounds were also detected as parts of hydroxyl benzoic acids and cyclic polyol (Kura´nska et al., 2019).
Moreover, G. senegalensis leaves were used for many purposes, such as pulmonary and respiratory problems, colic and diarrhea, syphilis, beriberi, leprosy, rheumatism, diuresis, impotency, and expurgation (Anka et al., 2020). Many bioactive ingredients have been known to make the G. senegalensis active as antioxidants. It also had been reported to have high contents of polyphenols, tannins, flavonoids, glycosides, and essential oils (Gurama et al., 2020). This report contradicted the findings of the present study that G. senegalensis leaf extracts and L. lanceolata leaf extracts had very low contents at F (15, 32) 6,676, at p value < 0.0001 with curve fitting (R 2 0.9993), as shown in Figure 4 (Ja'afar et al., 2013). L. lanceolata was quantified and determined to have reasonable contents (Mustapa et al., 2018) of phytochemicals such as alkaloids, flavonoids, phenolic, glycosides, saponins, and tannins, which contradicted the present findings. Nevertheless, ethnopharmacology is a scientific discipline that is concerned with searching for substitute drugs in medicinal plants that have active ingredients to address health abnormalities. Hence, many medicinal plants' properties were explored (Malgwi et al., 2019). The results of the various studies either confirmed or contradicted one another as stated by Malgwi et al., 2019;Khobe et al., 2017;SundayEne-OjoAtawodi et al., 2010. The methanolic extracts still indicated the presence of alkaloids, cardenolides, phlorotannins, steroids, tannins, and terpenoids (Malgwi et al., 2019). Nonetheless, polyphenols, guirenone, alkaloids, rhamnetin potentialities, as discovered using different techniques as described by (SundayEne-OjoAtawodi et al., 2010), were also present in L. lanceolata, despite their low contents, which differed from the present study. Subsequently, S. birrea stem (bark) methanolic extract, S. birrea stem (bark) ethanolic extract, V. nilotica ethanolic pods extract, and V. nilotica methanolic pod extracts happened to be the ones with reasonable phenolic contents, being significantly different from the rest of the plants extracts including their chloroform extracts. Notably, the least content was captured in L. lanceolata leaf extracts as depicted. Teke et al. (2011) have revealed that the n-hexane fraction appeared to have the least antibacterial action and the ethyl acetate fraction was the most active one against the Staphylococcus aureus. This was similar to the findings of the present study of 2.16 g S. birrea ethanolic stem (bark) crude extract with a procured yield of 10.8%. The same extract yielded residue of n-hexane of 0.165 g and a fraction percentage yield of 2.05%; 0.0133 g chloroform with a fraction percentage yield of 1.65%; 0.2645 g ethyl acetate with a fraction percentage yield of 32.88%. The final recovered yield was 0.4711 g, with the final % yield recovered being 58.5654, after partitioning as depicted in Figure 1. The same n-hexane fraction was the least followed by the chloroform fraction and again the ethyl acetate fraction extract of S. birrea stem (bark) was the active fraction against S. typhi isolate, as shown in Figure 3.
Subsequently, the 10 mg/ml of the S. birrea stem (bark) ethyl acetate fraction extract was the MIC and also no MBC on the fraction extract. It, therefore, inhibited the growth of the S. typhi but did not kill it, which was in accordance with the findings of Teke et al. (2011), where the antimicrobial actions were notably attributed to the presence of the identified compounds in both the crude extract and its fraction. Moreover, the work of Mushore & Matuvhunye (2013) found that ethyl acetate and the methanolic extracts had significant zones of inhibition close to that of the standard, and the activity of the water extract provided a basis for using water extracts in treating ailments traditionally and effectively. The latter agreed with some parts of the present study, whereas other parameters differed.
Nevertheless, the majority of the traditional herbal medications used in Africa are provided by practitioners who live within the populations. They trusted the system over time and are willing to assist patients with their knowledge and skills, sometimes at the least costs (Aliyu et al., 2015). Most of these herbal medicines are procured in their crude forms, although some pre-packaged pharmaceutical forms also exist. Evidently, interest in various traditional practices now exists among practitioners of modern medicine and there are a growing number of practitioners of Natural product unknown ID w stats online and local database searches (untargeted food research ID workflow with statistics: Detect and identify unknown compounds with differential analysis). Perform retention time alignment, unknown compound detection, and compound grouping across all samples. Predict elemental compositions for all compounds and hide chemical background (using blank samples). Identify compounds using MZCloud (ddMS2 and/or DIA), ChemSpider (exact mass or formula), and local database searches against mass lists (exact mass with or without RT) and mzVault spectral libraries. Perform spectral similarity search against MZCloud for compounds with ddMS2. Apply mzLogic to rank order structure candidates from ChemSpider and mass list matches. Apply spectral distance scoring to ChemSpider and mass list matches and perform differential analysis on detected compounds Frontiers in Chemistry | www.frontiersin.org July 2021 | Volume 9 | Article 670530 9 traditional, indigenous, or alternative systems (Aliyu et al., 2015). Solvents play important roles in making extracts to be more potent against the test organisms. Ethyl acetate and methanolic extracts had significant zones of inhibition as stated by Mushore and Matuvhunye (2013), which conformed to the present study, with some distinctive evidence that the isolate was susceptible to the positive control (ciprofloxacin, 30.33 ± 0.0).
Moreover, bioassay-guided fractionation has been a technique for plant extract profiling of bioactive compounds for screening potential sources of new drugs of biological importance. The fractionation procedure, therefore, is employed for better screening of natural products in plant extracts (Malviya, 2019). It has been confirmed that the bioassay-guided fractionation of the methanolic extract of E. triplinerve revealed active antimicrobial components in some solvents, notably hexane, dichloromethane, and ethyl acetate against Escherichia coli at 16 and 31 mg/ml and against Pseudomonas aeruginosa 31 and 62 mg/ml MICs (Regina et al., 2015).
However, it is evident that most of the Enterobacteriaceae family contributed to the destruction of the epithelial tissues, which led to other abnormalities, not only gastroenteritis (Nakamoto et al., 2019). It had been proven that most Salmonella spp. causing gastroenteritis were foodborne, which indicated survival in warmer conditions, whereas viral gastroenteritis occurred in cooler conditions (Fletcher et al., 2015). Consequently, S. birrea stem bark, roots, leaves, and fruits contained a myriad of chemical components, which allowed people to use the plant as both a food and as a traditional medicinal plant for their ailments (Mariod and Abdelwahab, 2010).
Generally, bioactive ingredients like alkaloids, essential oils biterpenoids, and flavonoids were normally extracted via methanol and ethanol, which revealed sound actions against both Gram-positive and Gram-negative bacteria such as S. aureus (Mushore and Matuvhunye, 2013). Moreover, S. birrea has tremendous ethnotherapeutic attributes and pharmacological actions due to its bioactive ingredients, which act as medicines. The bioactive ingredients include polyphenols, tannins, coumarins, flavonoids, triterpenoids, and phytosterols. Its constituents have antidiarrheal, antidiabetic, anti-inflammatory, anti-microbial, antihypertensive, anticonvulsant, and anti-plasmodial effects (Ojewole et al., 2009). Its fruits are regarded as wild fruits for their uniqueness of nutritional values by containing ascorbic acid and other hydrocarbons. S. birrea seeds do possess some essential acids and oil, which also acted as ant-inflammatory, antidiabetic, analgesic, antiparasitic, antimicrobial, and antihypertensive agents (Mariod and Abdelwahab, 2010).
Importantly, plants derived antimicrobial agents are the best options for combating the increase of bacterial antibiotic resistance (Khameneh et al., 2019). Moreover, most of the S. birrea secondary metabolites were determined using liquid chromatography-mass spectroscopy and NMR, where notably, flavonoids were the majority fighting various ailments (Mc et al., 2017). As with the present study, (+)-catechin gallate with full matches of fragmentation rates was revealed with 153.0182 m/z and retention time of 12.811. Epigallocatechin gallate also had full matches of different fragmentation rates as revealed by both the respective structures, m/z (305.0667), and retention time [13.790-16.221]. These caused the plant extracts to be active and play important roles in curing many abnormal conditions such as chronic pains, depression, and Parkinson's disease (Zeratsky, 2019).
Nevertheless, epigallocatechin gallate served as a valuable compound found mostly in green tea and synthetic food as probable additives. It has been documented to prevent different growths of both Gram-positive and Gram-negative bacteria capable of spoiling food since it possessed food antioxidant actions (Mahdi et al., 2019). It has been reported that gallic acid has many important effects, which include antioxidant and anti-inflammatory activities, as well as being able to address gastrointestinal, neuropsychological, and cardiovascular disorders (Kahkeshani et al., 2019). These findings were in line with our results that gallic acid possessed full matches of fragmentation rates with m/z (169.0143) and retention time [13.653-19.889], which signified the action of the extract on the test organisms. This was subsequently supported by the work that stated "epicatechin compound was identified with varying molecular weight and retention times (m/z 169.0145 [M-H-289-272] and (m/z 425.0851 [M-H-304] fragmentation rates." It was also in agreement with the yielding of catechin unit due to the loss of a gallic acid during fragmentation, as stated by Shoko et al. (2018). Subsequently, another compound was identified as 9, 10, 13-TriHOME with a peak of m/z 211.1227 [M-H-45-57] due to the loss of a carboxylic acid group and butyl and water fragments. Its secondary fragments were detected at m/z 171.0814 [M-H-71-87] and m/z 283.0403 [M-H-29-18] from the ethyl fragment. This yield was responsible for the inhibitory action of the fraction (Shoko et al., 2018).
The present study revealed that N, N-dimethyl had m/z 122.0964 and retention time of 0.043 with its full matches fragmentation rates in the fraction extract of S. birrea. These were similar to the findings of Skarpeli-Liati et al., 2012. Vidarabine was identified by MZCloud with its full and best matches fragmentation rates of 99.99 with m/z 268.1049 and retention time of 0.148 among the identified novel compounds in the study first found in the S. birrea stem (bark), which possessed antimicrobial attributes. This was supported by the work of Sunil et al. (2010), who reported that this compound played an important role, especially in inhibiting viral DNA. Hence, it served as an antiviral component. Moreover, DL-isoleucine, m/z (132.1019) and retention time [1.296], and DL-norleucine, mz (86.0964) with retention time [1.601], and their respective fragmentation rates were also present in reasonable amounts according to our findings. They were also found to be part of the novel compounds discovered in the sample. We attributed the plant action against the bacterial isolate to their presence. We also reported an amino acid that was non-polar and aided in protein synthesis, which was very crucial in humans, that was found in the diet after ingestion. It could also be found in other organisms like bacteria and had an effect on diabetes (Binns et al., 2016). Moreover, DLnorleucine proved to be active against Alzheimer's disease (Binns et al., 2016). It was also revealed to be part of the novel compounds in this research. Indeed, no literature reported DL-norleucine to be part of S. birrea stem (bark) extract constituents.
Moreover, the present findings revealed that L-phenylalanine was the second-best in obtaining full matches with various fragmentation rates of 99.7, m/z (166.0863), and retention time [1.937] in the fraction extract, which conformed with the fact that L-phenylalanine is an essential amino acid-like other normal amino acids helping in the production of proteins and could also be obtained from food and other supplement sources like Marula. It is also regarded as aromatic and polar due to its benzyl chain (Zeratsky, 2019). It played an important role in curing many abnormal conditions such as chronic pains, depression, and Parkinson's disease (Zeratsky, 2019). In addition, meprednisone acetate has partial matches of the fragmentation rates, being the only compound with such attribute in this analysis, which is also a new novel compound, as it was never discovered in the extract and the fraction used together with m/z 119.0858 in retention time of 15.444. Lastly, gentisic acid is also one of the newly identified compounds, having full matches of fragmentations revealed by MZCloud and merely by ChemSpider with few structures, m/z 153.0193 and retention time of 15.734. The N-methyl-4piperidone, 1,8-diazabicyclo[5.4.0]undec-7-ene, 2-amino-1,3,4-octadecanetriol, isophthalic acid, and diisopropylethylamine are the remaining notable novel compounds identified in the S. birrea stem (bark) extract, as no literature revealed their presence, as shown in Table 1 and

CONCLUSION
In the present research, the phenolic contents of the V. nilotica pod extracts (210.063%) and S. birrea stem (bark) and methanolic and ethanolic extracts with gallic acid curve (R 2 0.9958) were significantly different between the G. senegalensis and the L. lanceolata leaf extracts at p value < 0.0001. Likewise, the flavonoid contents showed similar trends as the phenolic contents were determined with catechin value (R 2 0.9993). Moreover, the reasonable amounts of phenolic and flavonoid contents determined the actions of the individual plant, notably toward the development of many valuable pharmaceutical products.
Moreover, the ethyl acetate fraction of the S. birrea stem bark extract happened to have the highest yield of the entire subfractionated solvent extracts and is the most potent against S. typhi. It only inhibited the growth but did not kill the test organism. Different compounds were identified with their structures as revealed by both MZCloud and ChemSpider using LC-MS/LC-HRMS. Vidarabine was the best compound with full matches of fragmentation rates although only MZCloud revealed its structure, followed by the essential amino acids and flavonoids such as catechin gallate and epigallocatechin and other phenolics like gallic acids and gentisic acids with all full matches of fragmentation rates across. Most importantly, meprednisone acetate appeared to be the least compound with partial matches of the fragmentation rates as revealed by both methods. Vidarabine happened to be among the novel compounds found in the S. birrea stem (bark), with outstanding results as compared to the remaining novel compounds such as N-methyl-4-piperidone, 1,8-diazabicyclo[5.4.0]undec-7-ene, 2-amino-1,3,4octadecanetriol, or isophthalic acid. A reasonable amount of phenolic and flavonoid contents determined the actions of the individual plant.
Nevertheless, the listed compounds in Table 1 elucidated with the fact that some were unknown while some were known with different structural formulae, revealed different elution times, and retention times at different fragmentation rates. Moreover, some compound found in the S. birrea stem (bark) extract were first identified in this work. Lastly, such a plant needs to be studied further to see the effects of the various compounds found and their contents. Individual compounds would be further fully identified to perform an in vivo testing so as to ascertain and correlate with the present findings so far. Thus, the S. birrea stem (bark) fraction extract is an important plant product, which could lead to solutions to many human health problems.

DATA AVAILABILITY STATEMENT
The original data presented in the study are included in the article/Supplementary Material; further inquiries can be directed to the corresponding author.

AUTHOR CONTRIBUTIONS
MA was responsible for conceptualizations, conducted the investigation and formal analysis, wrote and prepared the original draft, reviewed and edited the manuscript; MA, MM and, MN contributed to the methodology; SC and J-HP acquired the resources; GR validated the data;; MA and GR wrote, reviewed, and edited the manuscript. All authors have read and agreed to the published version of the manuscript.