TY - JOUR AU - Edgar, Rebecca J. AU - Chen, Jing AU - Kant, Sashi AU - Rechkina, Elena AU - Rush, Jeffrey S. AU - Forsberg, Lennart S. AU - Jaehrig, Bernhard AU - Azadi, Parastoo AU - Tchesnokova, Veronika AU - Sokurenko, Evgeni V. AU - Zhu, Haining AU - Korotkov, Konstantin V. AU - Pancholi, Vijay AU - Korotkova, Natalia PY - 2016 M3 - Original Research TI - SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes JO - Frontiers in Cellular and Infection Microbiology UR - https://www.frontiersin.org/articles/10.3389/fcimb.2016.00126 VL - 6 SN - 2235-2988 N2 - Streptococcus pyogenes (Group A Streptococcus or GAS) is a hemolytic human pathogen associated with a wide variety of infections ranging from minor skin and throat infections to life-threatening invasive diseases. The cell wall of GAS consists of peptidoglycan sacculus decorated with a carbohydrate comprising a polyrhamnose backbone with immunodominant N-acetylglucosamine side-chains. All GAS genomes contain the spyBA operon, which encodes a 35-amino-acid membrane protein SpyB, and a membrane-bound C3-like ADP-ribosyltransferase SpyA. In this study, we addressed the function of SpyB in GAS. Phenotypic analysis of a spyB deletion mutant revealed increased bacterial aggregation, and reduced sensitivity to β-lactams of the cephalosporin class and peptidoglycan hydrolase PlyC. Glycosyl composition analysis of cell wall isolated from the spyB mutant suggested an altered carbohydrate structure compared with the wild-type strain. Furthermore, we found that SpyB associates with heme and protoporphyrin IX. Heme binding induces SpyB dimerization, which involves disulfide bond formation between the subunits. Thus, our data suggest the possibility that SpyB activity is regulated by heme. ER -