Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) Are Able to Induce Beneficial Immunomodulatory Effects in a Murine Model for Treatment of Toxoplasma gondii Infection

Infection by Toxoplasma gondii affects around one-third of world population and the treatment for patients presenting toxoplasmosis clinically manifested disease is mainly based by a combination of sulfadiazine, pyrimethamine, and folinic acid. However, this therapeutic protocol is significantly toxic, causing relevant dose-related bone marrow damage. Thus, it is necessary to improve new approaches to investigate the usefulness of more effective and non-toxic agents for treatment of patients with toxoplasmosis. It has been described that lectins from plants can control parasite infections, when used as immunological adjuvants in vaccination procedures. This type of lectins, such as ArtinM and ScLL is able to induce immunostimulatory activities, including efficient immune response against parasites. The present study aimed to evaluate the potential immunostimulatory effect of ScLL and ArtinM for treatment of T. gondii infection during acute phase, considering that there is no study in the literature accomplishing this issue. For this purpose, bone marrow-derived macrophages (BMDMs) were treated with different concentrations from each lectin to determine the maximum concentration without or with lowest cytotoxic effect. After, it was also measured the cytokine levels produced by these cells when stimulated by the selected concentrations of lectins. We found that ScLL showed high capacity to induce of pro-inflammatory cytokine production, while ArtinM was able to induce especially an anti-inflammatory cytokines production. Furthermore, both lectins were able to increase NO levels. Next, we evaluated the treatment effect of ScLL and ArtinM in C57BL/6 mice infected by ME49 strain from T. gondii. The animals were infected and treated with ScLL, ArtinM, ArtinM plus ScLL, or sulfadiazine, and the following parameters analyzed: Cytokines production, brain parasite burden and survival rates. Our results demonstrated that the ScLL or ScLL plus ArtinM treatment induced production of pro-inflammatory and anti-inflammatory cytokines, showing differential but complementary profiles. Moreover, when compared with non-treated mice, the parasite burden was significantly lower and survival rates higher in mice treated with ScLL or ScLL plus ArtinM, similarly with sulfadiazine treatment. In conclusion, the results demonstrated the suitable potential immunotherapeutic effect of ScLL and ArtinM lectins to control acute toxoplasmosis in this experimental murine model.

Infection by Toxoplasma gondii affects around one-third of world population and the treatment for patients presenting toxoplasmosis clinically manifested disease is mainly based by a combination of sulfadiazine, pyrimethamine, and folinic acid. However, this therapeutic protocol is significantly toxic, causing relevant dose-related bone marrow damage. Thus, it is necessary to improve new approaches to investigate the usefulness of more effective and non-toxic agents for treatment of patients with toxoplasmosis. It has been described that lectins from plants can control parasite infections, when used as immunological adjuvants in vaccination procedures. This type of lectins, such as ArtinM and ScLL is able to induce immunostimulatory activities, including efficient immune response against parasites. The present study aimed to evaluate the potential immunostimulatory effect of ScLL and ArtinM for treatment of T. gondii infection during acute phase, considering that there is no study in the literature accomplishing this issue. For this purpose, bone marrow-derived macrophages (BMDMs) were treated with different concentrations from each lectin to determine the maximum concentration without or with lowest cytotoxic effect. After, it was also measured the cytokine levels produced by these cells when stimulated by the selected concentrations of lectins. We found that ScLL showed high capacity to induce of pro-inflammatory cytokine production, while ArtinM was able to induce especially an anti-inflammatory cytokines production. Furthermore, both lectins were able to increase NO levels. Next, we evaluated the treatment effect of ScLL and ArtinM in C57BL/6 mice infected by ME49 strain from T. gondii. The animals were infected and treated with ScLL, ArtinM, ArtinM plus ScLL, or sulfadiazine, and the following parameters analyzed: Cytokines production, brain parasite burden and survival rates. Our results demonstrated that the ScLL or ScLL plus ArtinM treatment induced production of pro-inflammatory and anti-inflammatory cytokines, showing differential but complementary profiles. Moreover, when compared with non-treated mice, the parasite burden was significantly lower
This infection is asymptomatic and well tolerated for the majority of the infected people, but it can cause severe disease and high rates of morbidity and mortality for some groups of patients, as the immunocompromised individuals, such as for AIDS patients (Enzensberger et al., 1985;Bal et al., 2014), as well as when it occurs during pregnancy, because the parasite can cross placenta and cause congenital toxoplasmosis (Jones et al., 2001;Adams Waldorf and McAdams, 2013). Thus, the treatment of toxoplasmosis is required for these patients presenting high risk of severe tissue damage (Vijayalaxmi and Vishalakshi, 2000;Montoya and Liesenfeld, 2004;Elsheikha, 2008;Kaye, 2011;Rodriguez and Szajnman, 2012;Blader et al., 2015). If fetal infection is confirmed, the mother should be treated with a combination of sulfadiazine, pyrimethamine, and folinic acid (Montoya and Remington, 2008). Even though sulfadiazine and pyrimethamine are widely used, these drugs are highly toxic and may cause severe adverse effects (Montoya and Remington, 2008;Kaye, 2011). In fact, these drugs may result in bone marrow toxicity, including megaloblastic anemia or pancytopenia, which may be reversible or preventable in some patients with folate supplementation (Mori et al., 2011). In addition to cause these severe side effects, these drugs might not be able to reduce the parasitism, as T. gondii has shown to present resistance to sulfadiazine (Meneceur et al., 2008;Doliwa et al., 2013;Oliveira et al., 2016).
The immune response against T. gondii involves complex mechanisms of innate and adaptive immunity. A Th1-type immune response is observed during acute infection, involving synthesis of cytokines, as IFN-γ and IL-12 (Gazzinelli et al., 1994;Lang et al., 2007). Given that modulated immunity is critical to control the parasite burden (Dupont et al., 2012), the induction of an appropriate immune response just after infection constitutes an remarkable alternative for toxoplasmosis treatment.
It has been described in the literature that lectins from plants, such as ArtinM from seeds of jackfruit (Artocarpus integrifolia) and ScLL from the latex of the Euphorbiaceae Synadenium carinatum, when used as immunological adjuvants in vaccination protocols, can control parasite infections caused by Leishmania major, Leishmania amazonensis or Neospora caninnun (Panunto-Castelo et al., 2001;Teixeira et al., 2006;Afonso-Cardoso et al., 2007;Toledo et al., 2009;Cardoso et al., 2011).
Considering that it is necessary to improve new approaches to investigate the usefulness of more effective and non-toxic agents for treatment of patients with toxoplasmosis, in addition to the fact that ScLL and ArtinM have been previously used only in vaccination protocols for parasitic infections, the major aim of the present study was to evaluate whether these lectins could be also applicable as therapeutic agents to avoid the tissue damages occurring in consequence of T. gondii infection.

Animals
Female inbred C57BL/6 mice, aging 8-10 weeks, were obtained from Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil. Animals were maintained under standard conditions in the Animal Facility from this Institution. All procedures were conducted in accordance with the guidelines for animal ethics and the study received approval of the Ethics Committee for Animal Experimentation of the Institution (CEUA-UFU), under protocol # 058/14.
Lectins from Synadenium carinatum Latex (ScLL) and Artocarpus heterophyllus Seeds (ArtinM) Specimens from S. carinatum were harvested in Uberlândia, MG, Brazil. ScLL was obtained as previously described (Souza et al., 2005), with some modifications. Briefly, proteins were extracted from the fresh plant latex by gentle shaking with distilled water, for 24 h, at 4 • C. The mixture was centrifuged for 10 min at 10,000 g, filtered on 0.45 µm membranes and frozen at −20 • C for 48 h. After being defrosted and filtered again, the extract was submitted to affinity chromatography using immobilized D-galactose column on agarose (Pierce, Rockford, USA). The D-galactose-binding lectin (ScLL) was eluted with 0.2 M D-galactose (Sigma Chemical Co., St Louis, USA) in BBS, concentrated, and dialyzed using Amicon R Ultra-Filters 40 kDa (Merck, Göttingen, Germany). The protein concentration was determined (Bradford, 1976) and ScLL purity was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) stained with Coomassie Blue (Laemmli, 1970). ScLL aliquots were stored at −20 • C until be used in the experimental procedures.
The D-mannose-binding ArtinM lectin extracted from A. heterophyllus was obtained from seeds of jackfruit and purified by sugar affinity chromatography, as previously described (Bunn-Moreno and Campos-Neto, 1981;Roque-Barreira et al., 1986).

In vitro Assays
Bone Marrow-Derived Macrophages (BMDMs) Bone marrow-derived macrophages (BMDMs) were obtained from C57BL/6 mice as previously described (Marim et al., 2010). Briefly, the femurs from mice (n = 2) were removed and washed with sterile PBS in Petri dish. The cell suspensions were collected, filtered, and centrifuged for 10 min at 400 g. The pellets were suspended in RPMI-1640 media containing 15% of LCCM (L-cell Conditioned Media) from L929 cell culture and incubated for 24 h. Cells were washed again and suspended in medium containing 2% SBF (serum bovine fetal). Viable cells were counted in a Neubauer chamber, using the Trypan blue exclusion vital stain.

Experimental Infection and Treatment
Mice (n = 45) were orally infected with 10 cysts of T. gondii ME49 strain and treated with sulfadiazine or lectins. For survival analysis, 25 animals were used, while 20 animals were used for cytokine measurement levels. Thirty days after infection, the surviving animals from both experiments were euthanized and their brains collected for determination of the parasite burden by Real-time PCR.

Survival Analysis
Five groups (n = 5) of infected and treated mice were chosen for survival analysis. One group was treated with sulfadiazine (250 mg diluted in 400 mL of drinking water given to the animals for 6 days), whereas four groups were treated for 6 days, at 1-day intervals, intraperitoneally, according with the following preparations: 50 µg of ScLL; 50 µg of ScLL plus 1 µg of ArtinM; 1 µg ArtinM; or PBS only. These lectin concentrations were chosen based on previous studies (Cardoso et al., 2011(Cardoso et al., , 2012. Thirty days after infection the surviving animals were euthanized and their brains collected to determine the rates of parasitism burden.

Measurement of Serum Levels of Cytokines
The production of cytokines was assessed in blood samples from infected and treated mice, as well as before treatment and infection and at the end of the treatments, i.e., 7 days after the infection. The serum samples were stored at −70 • C until being analyzed for measurement of IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF cytokines by Cytometric Bead Array (CBA) (BD Bioscience, San Jose, USA), according to the manufacturer's instructions. Samples were analyzed under BD flow cytometry (FACSCanto II; BD Company, San Diego, USA), and data were analyzed by using FlowJo data analysis software (FlowJo, LLC, Ashland, USA). The results were expressed as mean concentration pg/mL.

Determination of Parasite Burden in the Brain Tissues
Parasite burden was determined in the brain tissues by quantitative real-time polymerase chain reaction (qPCR). Total DNA was extracted using Proteinase K (Promega Co., Madison, WI, USA), as described by Miller et al. (1988). Total DNA was quantified by UV spectrophotometry at 260 nm (ND1000 Spectrophotometer; NanoDrop Technologies, Wilmington, USA). Real-time PCR was performed using the 7500 Real-time PCR System (Applied Biosystems, Foster City, USA) and SYBR R Green was used to detect fluorescence in PCR reaction, according to the manufacturer's instructions (Invitrogen, San Francisco, USA). The reaction conditions followed the protocols using primer pairs, forward, 5 ′ -CACAGAAG GGACAGAAGT-3 ′ and reverse, 5 ′ -TCGCCTTCATCTAC AGTC-3 ′ ) for amplification of T. gondii, as previously described (Homan et al., 2000;Wahab et al., 2010).

Statistical Analysis
The data were analyzed using GraphPad Prism 6.0 software package (GraphPad Software Inc., San Diego, USA). Differences among experimental and control groups were analyzed using One-way ANOVA with Tukey's multiple comparison post-test. Data were expressed as mean ± standard deviation (SD). The Kaplan-Meier method was applied to estimate the percentage of mice surviving at each time point after infection and survival curves were compared using the Log-rank Mantel-Cox test. Values of P < 0.05 were considered statistically significant.

BMDM Viability after Treatment with ScLL or ArtinM
It was observed a dose-dependent cytotoxicity in BMDM treated with ScLL. These cells treated with higher concentration of ScLL (50 µg/mL) showed less than 50% of viability, whereas the total number of live cells was significantly increased when the ScLL concentrations were reduced, showing viability rates higher than 80% for doses equal or lower than 5.5 µg/mL the ( Figure 1A). In contrast, for ArtinM, the viability rates were higher than 90% even at the higher concentration of 1 µg/mL ( Figure 1B).

Cytokines Production in BMDM under ScLL or ArtinM Treatment
ScLL and ArtinM were able to induce the cytokines production by BMDM (Figure 2). Cells treated with ScLL in concentrations of 1.8; 0.61; or 0.20 µg/mL, it was observed strong IL-12 production, in similar levels to that induced by LPS stimulus (Figure 2A). On the other hand, ScLL treatment was not able to induce IL-10 production in this cell suspension ( Figure 2B). Regarding cells treated with ArtinM, it was found that this lectin was able to stimulate IL-12 production, and the concentration of 0.11 µg/mL induced the higher level of this cytokine, achieving values similar to the LPS stimulus ( Figure 2C). In addition, ArtinM was able to induce IL-10 production in a dosedependent manner, achieving the highest level at 1.0 µg/mL ( Figure 2D).

Nitrite Production in BMDM Treated with ScLL or ArtinM
Nitrite concentration was measured in macrophages treated with ScLL or ArtinM, as an indicator of NO production. It was observed that both ScLL and ArtinM were able to stimulate NO production, being the higher level obtained in a concentration of 1.8 µg/mL for ScLL, while the concentrations of 1 and 0.33 µg/mL of ArtinM were equally able to induce higher levels of NO production (Figure 3).
Cytokines Production by T. gondii-Infected C57BL/6 Mice Treated with ScLL and/or ArtinM Lectins Given the efficiency of both lectins to stimulate cytokines production in BMDM, it was investigated whether ScLL and/or ArtinM treatments could be able to induce cytokines production by C57BL/6 infected mice. Analyses of cytokine levels showed that the treatment protocols with ScLL and/or ArtinM, consisting of six doses of 50 µg of ScLL, or six doses of 50 µg of ScLL plus 1 µg of ArtinM, increased significantly the concentrations of Th1, Th2, and Th17 cytokines when compared with non-treated or sulfadiazine-treated animals (Figure 4). However, a different profile concerning cytokines production was observed when the animals were treated with different lectin combinations. It was observed an induction higher of pro-inflammatory response in ScLL treated animals group compared to the ScLL plus ArtinM group. Indeed, IL-2, IFN-γ, and IL-6 production by infected and treated mice with ScLL was higher in comparison with ScLL plus ArtinM, as well as with sulfadiazine or non-treated infected controls (PBS) (P < 0.05) (Figures 4A,C,E). In contrast, ArtinM plus ScLL treatment induces higher levels of regulatory cytokines with a significant increase of IL-4, IL-10, and IL-17 in relation to others groups (P < 0.005) (Figures 4B,D,G). No significant difference in TNF production was observed among the treatments with lectins or sulfadiazine ( Figure 4F). ScLL plus ArtinM demonstrated better balance of Th1/Th2 type of immunity response.

Parasite Burden in C57BL/6 Mice Infected by T. gondii and Treat with ScLL and/or ArtinM Lectins
Parasite burden was examined in the brain tissues of the animals submitted to the different experimental procedures. As shown in Figure 5, it was observed that the treatments with either ScLL or ArtinM plus ScLL significantly reduced the parasite load in the central nervous system compared to infected but non-treated group (P < 0.05). In addition, it was not observed significant differences of T. gondii-DNA levels among the groups receiving lectins or sulfadiazine treatments, suggesting the ScLL and ArtinM could indeed reduce T. gondii burden to the central nervous system with the effectiveness comparable to the traditional chemotherapy.

Survival Rates of C57BL/6 Mice Infected by T. gondii and Treated with ScLL and/or ArtinM Lectins
To certify whether the lectins treatments may interfere in the survival of C57BL/6 mice infected by T. gondii, when comparing to the groups of animals non-treated or submitted to chemotherapy, it was determined the survival rates 30-day post-infection. As shown in Figure 6, the animals submitted to the experimental treatment with lectins presented higher survival rates than the infected but non-treated mice. Interestingly, treatment with ScLL alone ensured a 100% survival of the animals, equal the survival rate observed to those animals submitted to the sulfadiazine treatment (Figure 6). Also, for the group treated with ScLL plus ArtinM, the survival rate was 80%, while the survival rate was 60% for the group of mice treated with ArtinM alone. The infected but non-treated group had the lowest survival rate of 20% (Figure 6), which was significantly lower than the rates determined to groups treated with ScLL, ScLL plus ArtinM or sulfadiazine (P < 0.05).

DISCUSSION
The strategies for toxoplasmosis treatment still show serious limitations, particularly due to the high degree of toxicity that has been associated with the current chemotherapy (Yeo et al., 2016). Also, the possibility to occur parasite drug resistance is an additional drawback to treat this disease based only on chemotherapy (Hui et al., 2015). Due the immunostimulatory effect of plant lectins, studies have been developed to implement of vaccination protocols using new agents to stimulate the immune system, as ScLL and ArtinM lectins extracted from plants (Cardoso et al., 2011(Cardoso et al., , 2012Souza et al., 2013). However, there is no previous study analyzing the therapeutic use of ScLL and ArtinM against T. gondii infection.
The association of sulfadiazine with pyrimethamine constitutes a protocol of treatment extensively used for toxoplasmosis, considering that it has been demonstrated to be highly effective against T. gondii tachyzoites, but it may cause severe hematological side effects, among other type of toxicities (Bosch-Driessen et al., 2002;Chêne and Thiébaut, 2009;Andrews et al., 2014). Previous in vitro and in vivo studies showed ScLL and ArtinM can be safely administered on murine models (Panunto-Castelo et al., 2001;Afonso-Cardoso et al., 2007;Cardoso et al., 2012). Furthermore, in vivo models demonstrated that ScLL presents no cytotoxic activity against J774 cells or peritoneal murine macrophages (Souza et al., 2005). In the present study, it was demonstrated that BMDM presented high rates of viability when treated with appropriate concentrations of ScLL and ArtinM lectins. Considering this framework, it is becoming very clear in the literature in last years that the beneficial effects of the lectins transcend the problems of the any potential side effect that could exist. Thus, the original use of lectins in vaccination protocols has been expanded to other applications. Hence, besides its use as therapeutic agents in infectious and parasitic diseases, lectins have been applied as new potential antineoplastic agents targeting apoptosis, autophagy, and anti-angiogenesis in preclinical or clinical trials for cancer therapeutics, and in neurodegenerative disorders, as well (Alegre-Maller et al., 2014;Bogoeva et al., 2014;Aminou et al., 2016;Barbosa-Lorenzi et al., 2016;Dar et al., 2016;Freitas et al., 2016;Ricci-Azevedo et al., 2016).
The role of cytokines in the immune response against T. gondii is well known and it has been extensively demonstrated that the regulation on Th1 pattern is necessary to control inflammatory damage on tissues (Gazzinelli et al., 1994(Gazzinelli et al., , 1996Lang et al., 2007). T. gondii control requires a fine-tuning induction of proinflammatory and anti-inflammatory cytokines, such as IL-12, IFN-γ, andIL-10 (Gazzinelli et al., 1991, 1996), but the tachyzoite stage of the parasite is able to subvert the immune response, being able, for instance, to blockade NF-κB nuclear translocation and actively interfere in the IL-12 production (Yarovinsky, 2014;Kim et al., 2016). Previous studies have been shown the ability of ScLL and ArtinM to induce the production of cytokines, when used as immunological adjuvants in vaccination protocols (Cardoso et al., 2011(Cardoso et al., , 2012. In fact, when these lectins are used in murine models, they lead to the production of Th1, as well as Th2 cytokines, and these effects are important to avoid the deleterious consequences of unbalanced inflammatory cytokine production (Cardoso et al., 2011(Cardoso et al., , 2012. In the present study, it was demonstrated that ScLL could stimulate in vitro IL-12 production by macrophages, but not IL-10, corresponding to an induction of Th1 pattern immune response, which is necessary, but not sufficient, to control T. gondii infection. However, for ArtinM-stimulated macrophages, it was observed the production of both IL-10 and IL-12. Interestingly, we also observed that the lowest ArtinM dose resulted in the highest IL-10 production. Nitric oxide produced by macrophages has been demonstrated to be involved in anti-parasitic effects against various protozoa parasites, as well as in pathogenesis, including during T. gondii infection (Dincel and Atmaca, 2016). In the present study, it was observed that NO levels increased in macrophages stimulated in vitro with both ScLL and ArtinM. It has been described in murine models that, when the animals undergo the acute stage of infection by T. gondii, the NO production contributes to the tachyzoite-bradyzoite stage conversion (Ihara and Nishikawa, 2014). Our data showed that the non-toxic doses of ScLL as ArtinM are able to stimulate nitric oxide production by macrophages, which may be a relevant event to decrease T. gondii replication.
In the present study, it was established an intraperitoneal treatment protocol for toxoplasmosis, considering the results previously described (Cardoso et al., 2011(Cardoso et al., , 2012. Once high concentration of ScLL showed cytotoxicity, it was evaluated different doses of this lectin in mice to determine the occurrence of side effects, as lethality test in mice. For this purpose, the animals received six doses of PBS or ScLL (50,20,10,and 5 µg). The animals were observed during 30 days for the pattern of morbidity (temperature, weight loss, mobility in the cage) and at the end of that period all the treated animals showed no alteration of their physical conditions (Data not shown).
Next, we investigated the capacity of the ScLL and ScLL plus ArtinM to increase cytokine production in infected animals. It was observed in the groups of animals treated with ScLL alone or with ScLL plus ArtinM increased levels of both pro-inflammatory and anti-inflammatory cytokines, showing differential, but complementary profiles. Immunostimulatory effects of ScLL and ArtinM leading to increase levels of IFN-γ, IL-6, TNF, IL-12, and IL-10 have been described for experimental designs set up in vaccination protocols in murine models (Afonso-Cardoso et al., 2007;Cardoso et al., 2011). Here, we are demonstrating for the first time the cytokine production profile, induced by T. gondii infected mice and treated with ScLL and ArtinM. Taken into account that it is necessary new treatment alternatives for toxoplasmosis, due to the limitations of the current protocols, as their toxic effect and development of FIGURE 4 | Effects of sulfadiazine and lectin treatments in the levels of IL-2 (A), IL-4 (B), IFN-γ (C), IL-10 (D), IL-6 (E), TNF (F), and IL-17 (G) cytokines in serum samples from C57BL/6 mice. Results are expressed as mean ± SD of the cytokine levels in pg/mL. Statistically significances were determined by comparison of the values obtained in the infected and treated groups with non-treated and non-infected mice. Statistical test was performed using One-way ANOVA and Tukey's multiple comparison post-test. *Indicates significant differences (P < 0.05).
parasites resistance (Antczak et al., 2016), the treatment exploring the immunostimulatory ability of lectins could be considered a rational alternative, since it is based on the induction of defense mechanisms of the host against T. gondii infection.
Following the cytokine analysis, it was investigated in the present study the parasite burden in brain tissues of treated mice, using quantitative PCR (qPCR) to detect specific T. gondii DNA. As expected, the highest parasite load was observed in non-treated mice. The parasite burden did not show significantly difference between mice treated with sulfadiazine or lectins, revealing the potential ability of lectins in parasite control. Sulfadiazine is effective for toxoplasmosis treatment, however, it has harmful effects to the organism. Currently, toxoplasmosis is still treated with the synergistic combination of sulfadiazine and pyrimethamine or triple sulfonamides, which cause bone marrow damage, in addition to the potential life-threatening allergic reactions. For these reasons, alternative drugs as treatment options, including azithromycin, clarithromycin, dapsone, and artemisinin, have been studied for toxoplasmosis treatment, but an extensive number of side effects still persists (Araujo et al.,FIGURE 5 | Parasite burden in the brain tissues from treated mice after 30 days of infection with T. gondii ME-29 strain and analyzed by quantitative real-time PCR. Statistical significances were calculated by comparison the parasitic DNA values among the treated and non-treated groups of mice. Statistical test was performed using One-way ANOVA and Tukey's multiple comparison post-test. *Indicates significant differences (P < 0.05).
FIGURE 6 | Survival curve of C57BL/6 mice after infection with T. gondii ME49 strain. Animals were treated with ScLL, ArtinM, ScLL plus ArtinM, or sulfadiazine during 6 days, one time a day. As control, animals were infected with the same strain, but treated with phosphate-buffered saline (PBS) only. The survival curves were compared using the Log-rank Mantel-Cox test. Values of P < 0.05 were considered statistically significant. *Indicates significant differences (P < 0.05).
1992a,b; Bosch-Driessen et al., 2002;D'Angelo et al., 2009;de Oliveira et al., 2009;Yan et al., 2013;Rostkowska et al., 2016). Thus, it is necessary to investigate alternative approaches to find efficient and well-tolerated therapeutic agents against T. gondii infection. In the current study, it was demonstrated that treatment protocols using ScLL or ArtinM plus ScLL were able to reduce parasite burden for C57BL/6 mice recently infected by ME49 strain of T. gondii without side effects.
It has been extensively described in the literature that C57BL/6 mice are highly susceptible to infection by T. gondii, as they are not able to efficiently control the acute phase of infection, presenting high rates of mortality (Sánchez et al., 2015). In the present study, it was observed a significant protective effect of ScLL and ArtinM treatments in mice orally infected by ME49 strain of the parasite. In fact, when compared to non-treated group of animals, high percentages of treated animals survived during the acute phase of T. gondii infection. Regarding the different experimental groups, C57BL/6 mice treated with ScLL alone or ScLL plus ArtinM presented survival rates of 100 and 80%, respectively, in contrast with non-treated animals, which presented significant lower survival rate.
Altogether, the results obtained in present study indicate important immunostimulatory effects of ScLL and ArtinM that was able to control T. gondii infection. Further studies will be necessary to evaluate different protocols to optimize the efficacy of ScLL and ArtinM lectins on this protozoan infection, particularly those carry out with different parasite genotypes.

AUTHOR CONTRIBUTIONS
LS was involved in lectin preparation, cell culture, animal procedures, cytokine, and antibody assays, determination of brain parasite load for qPCR and preparation of the manuscript draft. ER participated in mouse infection and treatment procedures, cytokine and antibody assays. SS and MS were involved in inbred mouse maintenance, cytokine and antibody assays, and statistical analysis. FS was involved in the parasite maintenance in cell culture, T. gondii antigen preparation and lectin preparations. TM and JM were involved in the experimental design, data analysis, and revision of the manuscript. All authors read and approved the manuscript.