Impact Factor 4.300

The 5th most cited open-access journal in Microbiology

This article is part of the Research Topic

Pathogenesis of Leptospira

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2018.00063

ROLE OF MURINE COMPLEMENT COMPONENT C5 IN ACUTE IN VIVO INFECTION BY PATHOGENIC LEPTOSPIRA INTERROGANS

Íris A. Castro1,  Lorena Bavia1, Tatiana R. Fraga1, Mariane T. Amano1,  Leandro C. Breda1,  Adriana P. Granados Martinez1, Ana Maria G. Isaac2, Silvio A. Vasconcellos3 and  Lourdes Isaac1*
  • 1Department of Immunology, University of São Paulo, Brazil
  • 2Institute of Tropical Medicine, University of São Paulo, Brazil
  • 3Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brazil

Leptospirosis is considered one of the most important zoonosis worldwide. The activation of the Complement System is important to control dissemination of several pathogens in the host. Only a few studies have employed murine models to investigate leptospiral infection and our aim in this work was to investigate the role of murine C5 during in vivo infection, comparing wild type C57BL/6 (B6 C5+/+) and congenic C57BL/6 (B6 C5-/-, C5 deficient) mice during the first days of infection. All animals from both groups survived for at least 8 days post-infection with pathogenic Leptospira interrogans serovar Kennewicki strain Fromm (LPF). At the third day of infection, we observed greater numbers of LPF in the liver of B6 C5-/- mice when compared to B6 C5+/+ mice. Later, on the sixth day of infection, the LPF population fell to undetectable levels in the livers of both groups of mice. On the third day, the inflammatory score was higher in the liver of B6 C5+/+ mice than in B6 C5-/- mice, and returned to normal on the sixth day of infection in both groups. No significant histopathological differences were observed in the lung, kidney and spleen from both infected B6 C5+/+ than B6 C5-/- mice. Likewise, the total number of circulating leukocytes was not affected by the absence of C5. The liver levels of IL-10 on the sixth day of infection was lower in the absence of C5 when compared to wild type mice. No significant differences were observed in the levels of several inflammatory cytokines when B6 C5+/+ and B6 C5-/- were compared. In conclusion, C5 may contribute to the direct killing of LPF in the first days of infection in C57BL/6 mice. On the other hand, other effector immune mechanisms probably compensate Complement impairment since the mice survival was not affected by the absence of C5 and its activated fragments, at least in the early stage of this infection.

Keywords: Leptospira, complement system C5, murine model, Immunity, Infection Control

Received: 21 Sep 2017; Accepted: 13 Feb 2018.

Edited by:

Nadia Benaroudj, Institut Pasteur, France

Reviewed by:

Ricardo M. Gomez, Instituto de Biotecnologia y Biologia Molecular, CONICET-UNLP, Argentina
Marcelo Hill, Institut Pasteur de Montevideo, Uruguay
Alan J. McBride, Universidade Federal de Pelotas, Brazil  

Copyright: © 2018 Castro, Bavia, Fraga, Amano, Breda, Granados Martinez, Isaac, Vasconcellos and Isaac. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Lourdes Isaac, University of São Paulo, Department of Immunology, Avenida Prof. Lineu Prestes 1730, São Paulo, 05508-900, São Paulo, Brazil, louisaac@icb.usp.br