OmpR-Mediated Transcriptional Regulation and Function of Two Heme Receptor Proteins of Yersinia enterocolitica Bio-Serotype 2/O:9

We show that Yersinia enterocolitica strain Ye9 (bio-serotype 2/O:9) utilizes heme-containing molecules as an iron source. The Ye9 genome contains two multigenic clusters, hemPRSTUV-1 and hemPRST-2, encoding putative heme receptors HemR1 and HemR2, that share 62% amino acid identity. Expression of these proteins in an Escherichia coli mutant defective in heme biosynthesis allowed this strain to use hemin and hemoglobin as a source of porphyrin. The hemPRSTUV-1 and hemPRST-2 clusters are organized as operons, expressed from the phem−1 and weaker phem−2 promoters, respectively. Expression of both operons is negatively regulated by iron and the iron-responsive transcriptional repressor Fur. In addition, OmpR, the response regulator of two component system (TCSs) EnvZ/OmpR, represses transcription of both operons through interaction with binding sequences overlapping the −35 region of their promoters. Western blot analysis of the level of HemR1 in ompR, fur, and ompRfur mutants, showed an additive effect of these mutations, indicating that OmpR may regulate HemR expression independently of Fur. However, the effect of OmpR on the activity of the phem−1 promoter and on HemR1 production was observed in both iron-depleted and iron-replete conditions, i.e., when Fur represses the iron-regulated promoter. In addition, a hairpin RNA thermometer, composed of four uracil residues (FourU) that pair with the ribosome-binding site in the 5′-untranslated region (5′-UTR) of hemR1 was predicted by in silico analysis. However, thermoregulated expression of HemR1 could not be demonstrated. Taken together, these data suggest that Fur and OmpR control iron/heme acquisition via a complex mechanism based on negative regulation of hemR1 and hemR2 at the transcriptional level. This interplay could fine-tune the level of heme receptor proteins to allow Y. enterocolitica to fulfill its iron/heme requirements without over-accumulation, which might be important for pathogenic growth within human hosts.


INTRODUCTION
Yersinia enterocolitica, a member of the genus Yersinia in the family Enterobacteriaceae is a human enteropathogen which causes yersiniosis, i.e., gut-associated diseases such as enteritis, diarrhea, and mesenterial lymphadenitis (Bottone, 1997). Multiple virulence factors encoded by chromosomal and plasmid pYV-located genes are involved in Y. enterocolitica virulence (Cornelis, 2002). In addition, iron-acquisition systems are considered important pathogenicity determinants. The concentration of iron in the environment is critical for the control of bacterial metabolism. Iron limitation in the host can abolish bacterial growth, whereas a high intracellular iron concentration may damage bacterial cells due to the formation of harmful reactive oxygen species (ROS). Thus, the transport, storage, and metabolism of iron have to be tightly controlled to maintain iron homeostasis (Hantke, 2001).
A variety of mechanisms are employed by Y. enterocolitica to take up iron from the host body, including siderophore-mediated uptake and systems for acquiring iron from abundant heme or hemoproteins (Caza and Kronstad, 2013). Pathogenic strains can be divided into two groups: those producing the siderophore yersiniabactin (biotype 1B) and those unable to produce this siderophore but able to use ectogenic siderophores released by other bacteria, such as ferrioxamin B and E or ferrichrome (biotypes 2-5) (Heesemann, 1987;Bäumler et al., 1993). About 70% of the iron in the human host is present within heme and/or hemoproteins (hemoglobin, myoglobin, cytochromes). A heme uptake system was identified previously in Y. enterocolitica bio-serotype1B/O:8 strain WA-C Hantke, 1992, 1994). This system, involved in the acquisition and transport of the entire heme moiety into the cytoplasm, consists of the receptor HemR, an ATP-binding cassette (ABC) transporter HemTUV, and a putative heme-degrading protein HemS. The energy for heme uptake is transferred from the inner to the outer membrane via the TonB/ExbB/ExbD system (Krewulak and Vogel, 2011). The protein TonB spans the periplasm and can physically interact with a highly conserved region of the receptor HemR called the TonB box. A conformational change in the heme receptor caused by this interaction permits the transport of the ligand across the OM into the periplasm (Nau and Konisky, 1989;Braun et al., 1991). Gene clusters responsible for the uptake and transport of heme have been identified in several bacterial species, including Yersinia pestis (hmuRSTUV) (Thompson et al., 1999), Yersinia pseudotuberculosis (hmuRSTUV) (Schwiesow et al., 2018), Shigella dysenteriae (shu genes) , and Vibrio cholerae (hut genes) (Occhino et al., 1998), although their organization varies. The heme gene cluster of Y. enterocolitica bio-serotype 1B/O:8 strain WA-C contains six open reading frames (ORFs) hemPRSTUV, organized in an operon whose expression is enhanced by iron deprivation Hantke, 1992, 1994). To avoid high concentrations of free intracellular iron, the ferric uptake regulator (Fur) with Fe 2+ cofactor efficiently represses the transcription of appropriate genes. Fur is a global transcriptional regulator that tightly controls the transport, storage, and metabolism of iron in many Gram-negative bacteria (Hantke, 2001). In addition, Fur represses the expression of genes involved in the regulation of multiple cellular functions such as the oxidative stress response, energy metabolism, acid tolerance, and virulence (Hassett et al., 1996;Ochsner and Vasil, 1996;Bijlsma et al., 2002;van Vliet et al., 2003).
Bacterial cells are constantly challenged by various environmental stresses in their natural habitats. Y. enterocolitica faces several different challenges during infection and colonization of the human body. Efficient adaptation to changing environmental conditions is possible due to the activity of sensory regulators such as TCSs, e.g., the EnvZ/OmpR signaling pathway. This prototype TCS, first identified and characterized in non-pathogenic Escherichia coli K-12, consists of the transmembrane histidine kinase EnvZ and the response regulator OmpR. EnvZ senses changes in the environment, undergoes autophosphorylation and then a phosphate group is transferred to OmpR. Conformational changes in the phosphorylated OmpR allow it to bind to DNA as a dimer and modulate gene expression (Kenney, 2002). EnvZ/OmpR is involved in the transcriptional regulation, in a positive or negative manner, of several genes/operons of E. coli in response to changes in osmolarity, pH, temperature, and the concentration of nutrients in the environment (Slauch and Silhavy, 1989;Higashitani et al., 1993;Shin and Park, 1995;Vidal et al., 1998;Yamamoto et al., 2000;Jubelin et al., 2005). OmpR was recognized as a pleiotropic regulator that controls the expression of genes involved in many different cellular processes such as chemotaxis, motility, drug sensitivity, or acid resistance, and virulence of pathogenic bacteria (Bernardini et al., 1990;Chatfield et al., 1991;Shin and Park, 1995;Bang et al., 2002;Feng et al., 2003;Stincone et al., 2011).
The relationship between virulence and the activity of the OmpR protein has also been described for Y. enterocolitica O:8 (Dorman et al., 1989) and Y. enterocolitica 2/O:9 (our studies). We have shown that OmpR is involved in the control of various cellular processes and functions in Y. enterocolitica, including adhesion, invasion, motility, Yop production, biofilm formation, and multidrug and serum resistance (Brzostek et al., 2003(Brzostek et al., , 2007Raczkowska et al., 2010Raczkowska et al., , 2011aRaczkowska et al., ,b, 2015Brzóstkowska et al., 2012;Skorek et al., 2013). Taken together, these findings have revealed the important role of OmpR in remodeling the cell surface and in the adaptation of Y. enterocolitica to different environmental niches, including the host body.
Here, we demonstrate that the Y. enterocolitica Ye9 genome contains two multigene clusters, hemPRSTUV-1 and hemPRST-2, encoding homologous heme receptor proteins HemR1 and HemR2. Furthermore, we show that both clusters are organized as operons that are negatively regulated by Fur. More importantly, OmpR directly represses the expression of hemR1 and hemR2 through interaction with binding sequences located in the promoter regions of their respective operons. In addition, a zipper-like RNA structure closely resembling a FourU RNA thermosensor was recognized within the hemR1 5 ′ -untranslated region (5 ′ -UTR), but hemR1 expression was not subject to thermoregulation at the post-transcriptional level. Our results suggest that OmpR controls HemR1 expression in both the absence and presence of the Fur repressor. Finally, we evaluated the importance of both heme receptor proteins in iron/heme acquisition by Y. enterocolitica and E. coli cells.

Assay for Utilization of Heme-Containing
Compounds as an Iron Source Y. enterocolitica strain Ye9 grown under iron-depleted conditions was tested for the ability to obtain iron from heme-containing compounds. Initially, the growth yield (OD 600 ) of this strain in LB supplemented with the iron chelator (DPD) to final concentrations of 0, 100, 150, 200, and 300 µM, was assessed. Cultures were incubated at 26 or 37 • C for 28 h. Subsequently, heme and hemoglobin at final concentrations of 10 and 2.5 µM, respectively, were added to LBD (150 µM DPD) and the growth yield determined as above.
Growth of E. coli Strain SASX77 ( hemA Mutant) Carrying Plasmids Expressing Y. enterocolitica HemR1 or HemR2 Proteins The E. coli hemA strain SASX77, which is defective in ALA synthetase, was transformed with the plasmid pACYC184 or its derivatives pHEM1 and pHEM2, and maintained on LB agar supplemented with 50 µM ALA and Tet. Overnight cultures of these strains were adjusted to an OD 600 of 0.1 and 1 ml was harvested by centrifugation (2,400 × g, 2 min, RT). The pelleted cells were resuspended in 100 µl of 0.9% (w/v) NaCl, then mixed with 3 mL of 0.75% (w/v) agarose, and spread on LB agar plates. After solidification of the agarose, 5-mm diameter circles of Whatman filter paper (thickness 0.88 mm) were placed on the plates and these were wetted with 10 µl of different test solutions: 10 mM hemin, 0.1 mM hemoglobin, 0.9% (w/v) NaCl, or 50 mM ALA. The plates were photographed after incubation at 37 • C for 48 h.

CAS Assay
Chrome azurol S (CAS) agar plates, prepared as described previously (Schwyn and Neilands, 1987;Neilands, 1994), were used to monitor siderophore production. CAS is an iron-dye complex that changes color from blue to orange when iron (Fe 3+ ) is removed by the action of siderophores. Strains were grown overnight in LBD medium at 26 • C and 10 µl aliquots were spotted onto the plates. The plates were photographed after incubation at 26 • C for 48 h.

Molecular Biology Techniques
All DNA manipulations, polymerase chain reactions (PCRs), restriction digests, ligations, and DNA electrophoresis, were performed as previously described (Sambrook and Russell, 2001). Plasmid and genomic DNA were isolated using a Plasmid Miniprep DNA purification Kit and Bacteria & Yeast Genomic DNA Purification Kit (EurX), respectively. When the amplified fragments were used for cloning, PCR was performed using DreamTaq DNA polymerase or Phusion High-Fidelity DNA polymerase (Thermo Scientific). Oligonucleotide primers for PCR and sequencing were purchased from Sigma Aldrich and are listed in Table S2. DNA fragments amplified by PCR were purified using a PCR/DNA Clean Up kit (EurX). The plasmids used in this study are described in Table S1. DNA sequencing was performed by Genomed S.A. (Warsaw, Poland).
were first amplified from Y. enterocolitica Ye9 genomic DNA by PCR using the primer pairs hemP1F/hemP1R and hemP2F/hemP2R, respectively ( Table S2). The obtained amplicons were digested with the restriction endonucleases EcoRI/KpnI and cloned into the corresponding sites of reporter vector pCM132Gm [derivative of plasmid pCM132 (Marx and Lidstrom, 2001) containing a Gm resistance cassette] upstream of a promoterless lacZ gene. The resulting constructs were verified by PCR using the primer pair pCM132GmSpr1/pCM132Spr2 (flanking the EcoRI and KpnI sites) followed by sequencing of the amplicons. The two constructs, named pCM1 (p hem−1 ::lacZ) and pCM2 (p hem−2 ::lacZ), were introduced into E. coli BW25113. These constructs were also introduced into E. coli S17-1 λpir and transferred by conjugation into Y. enterocolitica Ye9N and the ompR mutant AR4. Selection of transconjugants was carried out on LB plates containing Gm and Nal (Ye9N) or Gm and Km (AR4). The presence of the plasmids in the Y. enterocolitica strains was confirmed by plasmid isolation and PCR with the primer pair pCM132GmSpr1/pCM132Spr2.

Construction of a HemR1 ′ -′ GFP Translational Fusion
To measure post-transcriptional regulation of hemR1 expression, a translational fusion with GFP was constructed in plasmid pFX-P (Schmidtke et al., 2013) using the Golden Gate technique (Engler et al., 2008). A DNA fragment carrying the 5 ′ -UTR of hemR1 plus the first 16 codons of the gene was amplified by PCR using the primer pair hemR-fw/hemR-rev and Ye9 genomic DNA as the template. In a separate PCR a fragment containing the lacZ promoter was amplified using primer pair OR181-placfw/OR182-plac-rv and plasmid pFX-P as template. The PCR primers contained BsaI sites and additional sequences designed to generate ends compatible with BsaI-cleaved vector pFX-P (Table S2). In a 20 µl Golden Gate cloning reaction, 40 fmol of vector were mixed with 40 fmol of each PCR product, 5 units of Eco31I (BsaI isoschizomer, Thermo Scientific), and 4.5 units of T4 DNA ligase (Thermo Scientific) in ligase buffer. The reaction was incubated at 37 • C for 1 h, 5 min at 50 • C, followed by 5 min at 80 • C, and then it was used to transform E. coli BW25113. Clones were selected on LB agar supplemented with Sp and their identity confirmed by PCR and plasmid DNA sequencing.

Construction of fur Deletion Mutants
The fur::Gm deletion mutants of Y. enterocolitica Ye9N and the ompR mutant AR4 were obtained by homologous recombination using suicide vector pDS132 (Philippe et al., 2004). A fur gene mutated by the insertion of a Gm R cassette was constructed by overlap extension PCR using primers listed in Table S2. As the first step, three DNA fragments were PCR-amplified using Y. enterocolitica genomic DNA (for flanking regions) or plasmid pBBR1MCS-5 Gm R (for the Gm R cassette) as the templates. Fragment A, extending from 403 bp upstream of fur to 6 bp within the ORF was amplified using primer pair Fur1/Fur2; fragment B, an 803-bp Gm R cassette was amplified using primer pair Fur2/Fur3; fragment C, comprising the last 20 bp of the fur ORF plus 557 bp downstream of this ORF was amplified using primer pair Fur5/Fur6. A mixture of these three amplicons was then used as the template with flanking primers (Fur1/Fur6) in a PCR to generate the mutagenic fragment (1,860 bp). This was purified, digested with XbaI, and then cloned into the corresponding site of suicide vector pDS132. The resulting plasmid pDSfur was propagated in E. coli S17-1 λpir, with selection on Cm and Gm, and then sequenced to confirm the absence of errors. This plasmid was introduced into two Y. enterocolitica strains, Ye9N and AR4, by biparental mating. Transconjugants created by single crossovers to integrate the allelic exchange plasmid into the Ye9N or AR4 genomes were selected in LB supplemented with Cm, Gm plus Nal (Ye9N), or Km (for AR4). Plasmid integration after a single crossover was verified by PCR. To force the second recombination, the transconjugant strains were plated on LB agar containing Gm and 10% (w/v) sucrose, and incubated at room temperature for 48 h. Sucrose-resistant colonies were screened for the loss of Cm resistance (encoded by the vector). The correct allelic exchange was verified for these fur mutants by PCR using the primer pair Fur0/Fur7 (Table S2) and by sequencing.

Gel Electrophoresis, Preparation of Cell Extracts, and Western Blotting
For immunological detection of the HemR1 and HemR1 ′ -′ GFP proteins, Y. enterocolitica strains were grown under the desired conditions and extracts prepared from equal numbers of cells. The protein concentrations in the extracts were determined using the RC-DC protein assay (Bio-Rad) and if necessary they were diluted in Laemmli buffer to achieve equal loading (Sambrook and Russell, 2001). Samples were separated by electrophoresis on 12% TGX Stain-Free FastCast Acrylamide gels. Each gel was then Stain-Free activated by a 5 min exposure to UV and imaged using a GE Healthcare AI600 Imager. In some experiments, the loading of equivalent amounts of protein was controlled by Coomassie blue staining of an identical gel run in parallel. Next, the proteins were transferred to nitrocellulose membrane (Amersham Protran Western blotting membrane, nitrocellulose, pore size 0.2 µM; GE Healthcare) using a wet electroblotting system (Bio-Rad). The blots were probed with polyclonal rabbit antibodies against HemR1 (1:10,000, generous gift of Jürgen Heesemann) or GFP (Sigma Aldrich, 1:10,000). Sheep anti-rabbit IgG, conjugated to horseradish peroxidase (HRP, Promega) was used as the secondary antibody (1:15,000). Positive immunoreaction was visualized using Clarity ECL Blotting Substrate (Bio-Rad) for HRP-based chemiluminescent detection (GE Healthcare AI600 Imager).
β-Galactosidase Assays β-Galactosidase assays were performed essentially as described by Thibodeau et al. (2004), using 96-well microtiter plates (Nest Sc. Biotech.) and a Sunrise plate reader (Tecan). Briefly, cultures grown to stationary phase were diluted to an OD 600 of 0.3-0.5 and 80 µl of each cell suspension were then mixed with 20 µl of POPCulture Reagent (EMD Millipore Corp) and incubated for 15 min to cause cell lysis. In the wells of a microtiter plate, 20 µl of each cell lysate were mixed with 130 µl of Z-Buffer and 30 µl of ONPG (4 mg/ml), as described by Miller (1992). For kinetic assays, the absorbance at 415 nm (relative to a blank) was measured at time intervals of 10 s, with 2 s of shaking before each reading. The assays were performed at 26 • C and monitored for up to 20 min. The β-galactosidase activity was expressed in Miller units calculated as described previously (Thibodeau et al., 2004). Each assay was performed at least in triplicate.

Electrophoretic Mobility Shift Assays (EMSAs)
For in vitro DNA-binding studies, recombinant OmpR-His 6 was expressed and purified as described previously (Nieckarz et al., 2016). Briefly, the N-terminal His-tagged OmpR protein (OmpR-His 6 , 29.78 kDa) was expressed from plasmid pETOmpR in E. coli BL21(DE3) and purified using Ni-NTA resin (Qiagen). The concentration of the purified OmpR protein was determined using the RC DC protein assay (Bio-Rad). DNA fragments comprising the regulatory regions upstream hem-1 and hem-2 clusters of the Y. enterocolitica Ye9 were amplified by PCR using primers listed in Table S2 with Ye9 genomic DNA as the template. The amplicons were purified using a Gene Matrix PCR/DNA Clean-Up kit (EurX) and the concentration of DNA was determined with a NanoDrop 2000. EMSA reactions (10 µl), contained 0.05 pmol of each DNA fragment, OmpR binding buffer (50 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM EDTA, 1 mM DTT, 20 mM MgCl 2 , 12% glycerol, 100 µg/ml BSA, 0.1% Triton X-100), and increasing amounts of OmpR-His 6 . After incubation at room temperature for 15 min, 2 µl of 30% (v/v) glycerol were added and the reactions were loaded onto a 4.2% native polyacrylamide gel (19:1 acrylamide/bisacrylamide, 0.2X TBE, 2% glycerol) that had been pre-run in 0.2X TBE running buffer for 40 min at 80 V. After loading, the electrophoresis was continued for 3 h at 110 V. The gels were then soaked in SYBRgreen 1X solution (Invitrogen) and visualized using a GE Healthcare AI600 imager.

Statistical Analyses
Statistical analyses were performed using Prism 7 software (v. 7.02, GraphPad). One-way ANOVA was used to determine statistically significant differences.

Y. enterocolitica Ye9 Utilizes Heme-Containing Molecules as an Iron Source
The wild-type strain Ye9 is a low-level pathogenic strain belonging to Y. enterocolitica bio-serotype 2/O:9. In contrast to more highly pathogenic Y. enterocolitica strains from bioserotype 1B/O:8, it lacks the high-pathogenicity island (HPI), a chromosomal cluster of iron-regulated genes involved in the biosynthesis of siderophore yersiniabactin (Ybt) and the uptake of Fe-Ybt into cells (Pelludat et al., 1998;Carniel, 2001). To study the ability of Y. enterocolitica strains to produce siderophores, the strains Ye9 and JB580v (bio-serotype 1B/O:8) were spotted onto agar containing CAS and incubated at 26 • C for 48 h ( Figure 1A). As anticipated, an orange halo was observed around the colonies of JB580v, demonstrating its ability to synthesize yersiniabactin ( Figure 1A), while Ye9 growth failed to produce any color change on the CAS plates, suggesting that it does not produce siderophores. Strain Ye9 was further characterized for its ability to utilize heme-containing molecules as an iron source. The growth yield of Ye9 incubated at 26 or 37 • C in LB medium supplemented with 100-300 µM of the iron chelator 2,2 ′ -dipyridyl (LBD) was significantly lower than in LB medium or LB with 10 µM FeCl 3 , and varied according to the chelator concentration ( Figure 1B). However, the addition of hemin (He, 10 µM) or hemoglobin (Hb, 2.5 µM) caused moderate stimulation of the growth of Ye9 in LBD (150 µM 2,2 ′ -dipyridyl) at 37 • C, demonstrating that this strain can utilize heme-bound iron from hemin or hemoglobin ( Figure 1C).

Identification of Two Multigenic Clusters Encoding Putative Outer Membrane Heme
Receptor Proteins HemR1 and HemR2 in the Genome of Y. enterocolitica Ye9 The hemR gene was previously recognized within the multigenic hemPRSTUV cluster responsible for the uptake and transport of hemin in highly pathogenic Y. enterocolitica (1B/O:8) strain WA-C (Stojiljkovic and Hantke, 1992). We established the presence of the hemPRSTUV cluster of Y. enterocolitica Ye9 (2/O:9) by PCR amplifications using primers designed from the Y11 (4/O:3) genome sequence (GenBank Acc. No. FR729477). Sequencing of these amplicons confirmed the presence of six ORFs hemPRSTUV-1 (hem-1 locus) ( Figure S1). The sequence of this gene cluster was identical to that found in the Y11 (4/O:3) genome and it shared 99% sequence identity with hemPRSTUV of strain 8081 of bio-serotype 1B/O:8 (data not shown). The Y. enterocolitica hemR1 gene encoding the outer membrane heme receptor protein HemR1 (687 amino acids) is situated downstream of the hemP1 ORF encoding a small protein HemP1 (64 amino acids) of uncharacterized function (Figure 2A). The amino acid sequences of the HemR1 protein of Y. enterocolitica (2/O:9) strain Ye9 and Y. enterocolitica (1B/O:8) strain 8081 share 99% identity (data not shown). The deduced amino acid sequences of the proteins encoded by hemSTUV-1 in Ye9 are similar to the equivalent sequences of proteins of the 8081 strain. FIGURE 1 | Y. enterocolitica Ye9 siderophore production and heme-containing molecule utilization. (A) Siderophore activity was assessed using CAS agar plates. No color change is seen on the plate carrying growth of Ye9. In contrast, a change in color from blue to orange is observed when iron is removed from the iron-CAS complex by the siderophore yersiniabactin produced by JB580v cells. (B) The growth yield of Ye9 determined by measuring the OD 600 after 28-h incubation at 26 or 37 • C in LB, LB with 10 µM FeCl 3 , LB with 100, 150, 200, or 300 µM 2,2 ′ -dipyridyl (DPD). (C) The growth yield of Ye9 in LBD medium (150 µM DPD) containing hemin (He, 10 µM) or hemoglobin (Hb, 2.5 µM). The graphs present the results of one of two separate experiments performed in triplicate.
HemS1 is possibly involved in the release of iron from heme, and HemTUV-1 comprise subunits of a periplasmic/inner membrane ABC heme permease (Stojiljkovic and Perkins-Balding, 2002).
Bioinformatic analysis using BLAST software revealed the presence of a second multigenic cluster hemPRST-2 (hem-2 locus), encoding putative hemin transporter protein HemR2, in the genomes of Y. enterocolitica strains of low (2/O:9) and high pathogenicity (8081 strain, 1B/O:8) (Figure 2A). As for hem-1, we determined the sequence of the hem-2 locus in Y. enterocolitica strain Ye9 ( Figure S1). Analysis of the structure of this novel heme transport locus revealed four intact ORF hemPRST-2 organized in an order similar to the hemin transport cluster hem-1. The predicted translation products of the hem-2 locus genes exhibit a high degree of amino acid sequence similarity to those encoded by the corresponding ORFs of hem-1 (HemP 52%, HemR 62%, HemS 53%, HemT 60% identity, respectively) ( Figure S2, Figure 2B). The nomenclature for the hem-2 genes at locus 2 follows that of the highly homologous hem genes at locus 1, and the putative proteins encoded are named HemP2, HemR2, HemS2, and HemT2, respectively. Interestingly, the lack of hemUV2 genes in this cluster suggests that the putative hem-2 heme permease is non-functional. This does not exclude the possibility that HemR2 might function as a receptor protein involved in heme uptake by utilizing components of the ABC transport system encoded by the hem-1 locus.
Alignment of HemR2 with the well-characterized HemR1 showed high amino acid sequence identity. These homologous proteins have typical TonB boxes at their amino-termini, conserved histidine residues, plus region V, which is characteristic of TonB-dependent proteins. These conserved motifs and residues were shown to be essential for heme transport through the receptor channel (Thompson et al., 1999) ( Figure 2B).

Genes Within the hemPRSTUV-1 and the hemPRST-2 Clusters Are Organized as Operons
There are six and four genes within the hemPRSTUV-1 (hem-1) and the hemPRST-2 (hem-2) clusters of Y. enterocolitica Ye9, respectively (Figure 2A). To determine whether the genes within these two loci might be transcribed as one transcriptional unit, sqRT-PCR analysis was performed on total RNA isolated from Y. enterocolitica Ye9 grown in iron-depleted medium (LBD). The locations of the primers used in this analysis are shown in Figures 3A,C and their sequences are given in Table S1. For the hemPRSTUV-1 cluster, the obtained RT-PCR products (815 and 739 bp) confirmed co-transcription of the hemPR-1 and hemRS-1 genes, respectively. The operon organization of hemPRSTUV-1 was conclusively demonstrated by the amplification of a 5,612-bp RT-PCR product ( Figure 3B).
The operon organization of the hemPRST-2 cluster was also examined by sqRT-PCR analysis using the primers (Table S1) shown in Figure 3C. RT-PCR products of the expected sizes (528 and 804 bp) confirmed the co-transcription of the hemPR-2 and hemRS-2 genes, respectively. However, a 3,692-bp RT-PCR product spanning the entire hemPRST-2 operon could not be obtained, despite repeated attempts. Therefore, co-transcription of the hemRST-2 genes was tested. The presence of a faint RT-PCR product of the expected size (2,082 bp) confirmed the operon organization of the hemPRST-2 cluster ( Figure 3D). Based on these data, it seems that the hemPRST-2 transcript might be particularly unstable or expressed at low levels at 37 • C. Control PCRs with the tested primers and Ye9 genomic DNA as template gave positive results in all cases (data not shown).  Table S2. 1, RThPR1F; 2, RThPR1R; 3, RThRS1F; 4, RThRS1R; 5, RThPV1R; 6, RThPR2F; 7, RThPR2R; 8, RThRS2F; 9, RThRS2R; 10, RThPT2R. (B,D) Agarose gel electrophoresis of sqRT-PCR products from hem clusters 1 and 2. Total RNA isolated from strain Ye9 grown in iron-depleted LBD medium (LB with the iron chelator 2,2 ′ -dipyridyl) at 37 • C was DNase I treated and then reverse transcribed with random hexamers. The obtained cDNA was used as a template in PCR reaction with pairs of primers shown in (A) and (C). RNA was used as the template in negative control reactions. M, GeneRuler 1 kb DNA Ladder.

Role of HemR1 And HemR2 Proteins in Heme and Hemoprotein Utilization by E. coli hemA Strain SASX77
Previous studies have shown that a plasmid containing hemPR, part of the hemin operon of Y. enterocolitica WA-C O:8, permits an E. coli strain lacking a functional hemA gene to use hemin as a porphyrin source Hantke, 1992, 1994). To examine the importance of the two Y. enterocolitica Ye9 HemR proteins in the utilization of hemin and hemoglobin as sources of porphyrin, they were introduced into the E. coli hemA strain SASX77, which is defective in ALA synthetase (auxotrophic for ALA, the heme biosynthetic precursor). The hemPR genes of the hem-1 and hem-2 operons with their native promoters were cloned into vector pACYC184 to obtain plasmids pHEM1 and pHEM2, respectively. These plasmids and the parent vector were introduced into E. coli SASX77. All transformant strains grew well in the presence of ALA, but no growth was observed on LB agar lacking this precursor.
To examine the effect of hemin or hemoglobin on the growth of strain SASX77 harboring plasmids containing hemPR1 or hemPR2, a plate assay was used to detect utilization of these porphyrin sources in iron-replete (LB) and iron-deficient conditions (LBD). The strains expressing the receptors HemR1 or HemR2 were able to utilize exogenously supplied hemin and hemoglobin as porphyrin sources (Table 1, Figure 4). However, growth of the SASX77/pHEM1 (HemR1 + ) strain around the hemin and hemoglobin discs was significantly stronger than that of strain SASX77/pHEM2 (HemR2 + ). Moreover, the HemR2expressing bacteria grew only as single colonies. Control strain SASX77/pACYC184 was unable to use either hemin or hemoglobin ( Figure 4A). In iron-depleted conditions, the zone of growth stimulation was broad (up to 30 mm in diameter), but the colonies were very small and needed more time to develop (up to a week, in contrast to 1-2 days for the strains growing on LB) (data not shown). These results strongly suggest that both HemRs participate in the acquisition of heme and hemoproteins in Y. enterocolitica Ye9, and that HemR1 is significantly more efficient than HemR2.
Next, the level of HemR1 receptor protein in the E. coli strain carrying pHEM1 was determined by Western blot analysis with anti-HemR1 polyclonal antibody ( Figure 4B). Cells of Y. enterocolitica Ye9 and of the E. coli strain carrying pACYC184 were used as a positive and negative control, respectively. All strains were grown in both iron-replete (LB+FeCl 3 ) and irondeficient (LBD) conditions. Extracts of cells grown to stationary phase at 37 • C were immunoblotted and a strong HemR1 signal was produced by the samples from the Ye9 strain and E. coli DH5α/pHEM1 grown in LBD medium. As expected, no immunoreactive band was observed in the case of E. coli/pHEM2. This result indicated the absence of cross-reactivity between anti-HemR1 antibodies and the protein HemR2. Since no HemR2 antibody was available it was not possible to confirm expression of this protein in the E. coli strain carrying plasmid pHEM2. This experiment also demonstrated the induction of HemR1 protein in Y. enterocolitica grown under iron starvation conditions, confirmed that this gene can be expressed in a heterologous system from a recombinant plasmid, and showed that this expression is repressed by iron.

OmpR Inhibits the Activity of the hemPRSTUV-1 and hemPRST-2 Promoters
The putative promoters p hem−1 and p hem−2 , located 42 and 43 bp upstream of the hemP gene in the hemPRSTUV-1 and hemPRST-2 operons, respectively, of Y. enterocolitica Ye9, were identified using the program BPROM (Solovyev and Salamov, 2011). Sequence analysis of the hemPRSTUV-1 and hemPRST-2 regions also led to the identification of an internal, putative promoter in the intergenic region between hemR and hemS. These findings corroborate the previously described locations of hemPRSTUV operon promoters in Y. enterocolitica WA-C (Stojiljkovic and Hantke, 1992), Y. pestis (hmu locus, Thompson et al., 1999), and Y. pseudotuberculosis (hmu locus, Schwiesow et al., 2018). In silico analysis revealed the presence of potential OmpR binding sites within the p hem−1 and p hem−2 promoters (see below), but not within the putative internal promoters, so their activity was not investigated further.
To examine the activity of the p hem−1 and p hem−2 promoters and the influence of iron and the regulator OmpR, we generated transcriptional fusions of the putative promoter sequences with the lacZ gene in vector pCM132Gm. The resulting plasmids pCM1 (p hem−1 ::lacZ) and pCM2 (p hem−2 ::lacZ) were introduced into E. coli BW25113 and two strains of Y. enterocolitica: the wildtype strain Ye9N (both fusions) and the isogenic ompR mutant AR4 (only fusion p hem−1 ::lacZ). The transformed strains were grown to stationary phase in LB or LBD and β-galactosidase activity was measured (Figure 5).
In the E. coli strains grown at 37 • C in both iron-replete or iron-depleted conditions the expression of the p hem−1 ::lacZ transcriptional fusion (pCM1) was significantly higher than that of p hem−2 ::lacZ (pCM2), indicating that p hem−2 is a weaker promoter. In addition, the expression of both p hem−1 ::lacZ and p hem−2 ::lacZ was upregulated in the presence of the iron chelator (∼3-fold and ∼2.5-fold, respectively), demonstrating inhibition by inorganic iron (Figure 5A).
To investigate whether the hem-1 and hem-2 operons are subject to OmpR-dependent regulation, p hem−1 ::lacZ and p hem−2 ::lacZ activity was measured in the Y. enterocolitica strains differing in the amount of OmpR grown at 26 or 37 • C in iron-replete or iron-depleted conditions. With the p hem−1 ::lacZ fusion, a ∼2-fold (26 • C) and ∼1.5-fold (37 • C) upregulation was observed under conditions of iron deficiency, confirming iron-dependent regulation of p hem−1 . Moreover, βgalactosidase activity was increased in the absence of OmpR at both studied temperatures in iron-replete or iron-depleted conditions ( Figure 5B). The effect of complementation of the ompR mutation in strain AR4 or overexpression of OmpR in the wild-type strain were studied by introducing plasmid pBOmpR carrying the wild-type copy of ompR. An increase in the level of OmpR caused a clear reduction in the expression of p hem−1 ::lacZ in cells grown in LB with FeCl 3 or LBD, showing that OmpR negatively regulates expression of this fusion. These results indicated that hem-1 expression is controlled by an ironand OmpR-dependent promoter located upstream of this operon. Both iron and OmpR participate in the negative regulation of hem-1.
To test the role of iron and OmpR in the regulation of p hem−2 ::lacZ, the wild-type strain Ye9N transformed with plasmid pCM2 was grown at 26 or 37 • C in iron-replete or iron-depleted conditions. A ∼1.5-fold increase in β-galactosidase activity was detected in wild-type cells grown at 26 • C under irondeficiency ( Figure 5C). However, the inhibitory effect of iron on p hem−2 ::lacZ expression was not observed at 37 • C, suggesting a more complex regulatory mechanism compared to the hem-1 promoter. Attempts to study the activity of the p hem−2 ::lacZ reporter fusion in the ompR mutant background were thwarted by the inability to transform strain AR4 with plasmid pCM2. Therefore, the wild-type ompR allele was introduced in trans (pBOmpR) into the Ye9N cells to monitor the effect of overexpression. The observed decrease in p hem−2 activity in the presence of ompR in multicopy in the wild-type strain grown FIGURE 5 | OmpR-dependent regulation of hem-1 and hem-2 operon expression. (A) Activity of the p hem−1 and p hem−2 promoters in E. coli BW25113 harboring the p hem−1 ::lacZ (pCM1) or p hem−2 ::lacZ (pCM2) fusion (Continued) FIGURE 5 | plasmids, compared with the same strain carrying parent vector pCM132. Strains were grown overnight in LB+FeCl 3 or LBD medium at 37 • C.
at 26 or 37 • C, suggested a negative role for OmpR in hem-2 expression. Interestingly, the inhibitory effect of iron was absent in the wild-type strain containing plasmid pBOmpR grown at 26 • C ( Figure 5C).

Impact of Fur and OmpR on HemR1 Receptor Production
The analysis of strains carrying the p hem−1 ::lacZ reporter fusion construct suggested that iron and OmpR negatively influence the expression of the hem-1 operon at the transcriptional level. We next examined whether this regulatory effect was observable at the protein level. Western blot analysis with a polyclonal anti-HemR1 antibody was used to detect the HemR1 protein in cell extracts prepared from the following Y. enterocolitica strains: wild-type Ye9N, ompR mutant AR4, Ye9N/pBOmpR, AR4/pBOmpR, and strains with empty vector pBBR1. All strains were grown to stationary phase at 37 • C in LB supplemented with FeCl 3 (iron-replete) or LBD (iron-depleted). As shown in Figures 6A,B, HemR1 protein production was significantly influenced by iron content, because HemR1 was undetectable in strain Ye9N grown under iron-replete conditions, and highly abundant in iron-depleted conditions. Moreover, HemR1 production was notably increased in the ompR mutant compared to the wild-type strain in both media. Plasmid pBOmpR carrying the wild-type ompR allele, used to complement the ompR mutation in strain AR4, caused a clear reduction in the level of HemR1, confirming that OmpR negatively regulates its expression. In addition, the strain Ye9N/pBOmpR, which overexpresses OmpR, produced less HemR1 than strain Ye9N in LBD medium ( Figure 6B). The presence of the empty vector pBBR1 in the wild-type strain and the ompR mutant, grown in LBD medium ( Figure 6B) did not have any impact on the level of HemR1. However, for some unknown reason, a difference in the abundance of HemR1 was observed in the ompR mutant vs. the ompR strain carrying the empty vector pBBR1, when grown in iron-replete conditions ( Figure 6A). These data corroborated the results of the transcriptional fusion analysis and suggested that the Fur may be involved in iron-dependent inhibition of hem-1 operon expression, and thus HemR1 production.
In order to determine whether OmpR controls the level of HemR1 expression independently of the Fur repressor, the fur gene was deleted in both the wild-type strain Ye9N (Ye9fur) and the ompR mutant (AR4fur). HemR1 levels were then examined by immunoblotting of cell extracts prepared from the fur, furompR and furompR/pBOmpR strains grown to stationary phase in LB supplemented with FeCl 3 or LBD at 37 • C ( Figure 6C). The Western blot data showed a high level of HemR1 production in the fur mutant background (independently of iron concentration), confirming that the Fur represses the biosynthesis of HemR1 in Y. enterocolitica. Interestingly, the strain also lacking OmpR (furompR mutant), grown in LBD medium, displayed a further increase in HemR1 protein abundance (ca. 70%), which suggested that OmpR inhibits the production of HemR1 independently of Fur. This effect was restored by complementation with plasmid pBOmpR carrying the wild-type ompR allele. Under iron-replete conditions the difference in HemR1 level between the fur and furompR mutants was less evident. These data suggested that iron may influence OmpR-dependent HemR1 synthesis irrespective of Fur.
In silico analysis of the hem-2 promoter region p hem−2 revealed a putative Fur binding site (FBS-2), as well as a potential OmpR binding site (OBS-2) in similar locations to FBS-1 and OBS-1. FBS-2 and OBS-2 are 74 and 45% identical to the E. coli consensus sequences, respectively ( Figure 7B).
The ability of OmpR to directly interact with the predicted OBS elements was tested in electrophoretic mobility shift assays (EMSAs) using DNA fragments with or without the putative OmpR-binding sites (Figures 7C-E). Three p hem−1 fragments were PCR-amplified with the pairs of primers listed in Table S2. Fragment F1 (415 bp) represented a sequence upstream of OBS-1, F2 (326 bp) carried OBS-1, while F3 (107 bp) was a sequence downstream of OBS-1. When purified recombinant OmpR was mixed with equimolar amounts of these three p hem−1 fragments, shifted OmpR/DNA complexes started to appear at an OmpR concentration of 0.65 µM, and their amount increased as the concentration of the protein was raised (Figure 7D). The formation of these complexes was related to the disappearance of fragment F2, so were attributed to specific binding to OBS-1.
The interaction of OmpR with the putative OBS-2 within the promoter region of hem-2 was examined by incubating increasing amounts of recombinant OmpR with fragment F4 (303 bp), the region of p hem−2 containing OBS-2, or p hem−1 fragment F2 (326 bp), carrying OmpR binding site OBS-1. Specific OmpR/F4 complexes were observed at an OmpR concentration of 1.31 µM, with the simultaneous disappearance of fragment F4. In comparison, the appearance of OmpR/F2 complexes occurred at the lower OmpR concentration of 0.65 µM (Figure 7E). A 16S rDNA fragment (amplified with primer pair 16SF/16SR, Table S2), to which OmpR is unable to bind, was used as a negative control in both experiments. These results suggested that transcription of the hem-1 and hem-2 gene clusters is directly regulated by OmpR, and this regulation requires OmpR binding to specific sites in their promoter regions.
The Impact of Temperature on Post-transcriptional HemR1 Expression Y. enterocolitica HemR1 is a homolog (69% amino acid identity) of the S. dysenteriae ShuA receptor required for the utilization of heme as a source of iron (data not shown). Recently, thermoregulation of ShuA protein expression under iron-limited conditions was demonstrated, with low ShuA abundance at 25 • C increased to high levels at 37 • C ( Kouse et al., 2013). Within the shuA 5 ′ -UTR a regulatory element was recognized that closely resembles a FourU RNA thermometer: a zipperlike RNA structure that occludes the Shine-Dalgarno sequence at low temperatures, thus blocking translation of the shuA transcript.
Both HemR1 and ShuA are encoded within hemin transport loci, but the organization of these multigenic clusters differs considerably . The hemR1 gene of Y. enterocolitica is located 172 bp downstream of hemP1, the first gene of the hemPRSTUV-1 operon. There is no hemP1 homolog in the hemin transport locus in S. dysenteriae. Moreover, the intergenic non-translated hemP1-hemR1 region (172 nt) is present in polycistronic mRNA of hem-1. In comparison, the predicted promoter of shuA is located 328 bp upstream of the translation start site in a monocistronic transcript.
surrounding regions (Figure 8A). No similar secondary structure was detected in the 5 ′ -UTR of hemR2 (data not shown). The impact of temperature on HemR1 expression was assessed by immunoblotting of cell extracts prepared from the fur mutant following growth in LB medium to stationary phase at 26 or 37 • C ( Figure 8B). Identical levels of HemR1 were detected in the tested strain, irrespective of the growth temperature, which indicated that HemR1 production is not thermoregulated in Y. enterocolitica Ye9.
To further examine the potential thermoregulation of HemR1 expression at the post-transcriptional level, a plasmid pFX-P lac -hemR1, which carries the p lac promoter linked to the 5 ′ -UTR and first 16 codons of hemR1 fused in frame with gfp, was constructed. Plasmid pFX-P lac -hemR1 and control plasmids pFX-0, containing promoterless gfp, and pFX-1, carrying gfp fused to the p lac promoter (Schmidtke et al., 2013), were introduced into E. coli BW25113. Cultures of these strains were grown to stationary phase in LB medium at 26 or 37 • C and green fluorescence intensity was measured ( Figure 8C). Analysis of the strain carrying the hemR1 ′ -′ gfp fusion revealed a decrease in hemR1 expression at 26 • C relative to 37 • C, however a slight reduction in fluorescence at this lower temperature was also observed for the control strain carrying pFX-1. In parallel, the impact of growth temperature on the GFP level was assessed by immunoblotting with an anti-GFP antibody ( Figure 8D). The Western blot data corresponded well with the results of the fluorescence assay. A higher level of HemR1 ′ -′ GFP fusion protein was produced at 37 • C compared to 26 • C, which may indicate that temperature-dependent post-transcriptional regulation of hem-1 occur in E. coli. However, we could not exclude that observed effect may be explain also by the increased metabolic activity at the higher temperature.

DISCUSSION
The acquisition of iron/heme is necessary for pathogenic bacteria to grow within the host and to survive during the progression of an infection (Mietzner and Morse, 1994;Lee, 1995;Braun et al., 1998). On the other hand, both iron and heme are toxic at high concentrations (Anzaldi and Skaar, 2010). Thus, tight regulation of iron/heme acquisition is essential to fulfill the iron requirements of a pathogen while preventing harmful overaccumulation.
Studies on the role of OmpR in pathogenic Yersinia strains have provided evidence that this response regulator might be involved in the adaptation of yersiniae to diverse environmental conditions, which allows the bacteria to grow in distinct niches within and outside the host body (Brzostek et al., 2003(Brzostek et al., , 2007Raczkowska et al., 2011aRaczkowska et al., ,b, 2015Brzóstkowska et al., 2012;Skorek et al., 2013). In a recent shotgun proteomic study we revealed that the regulator OmpR affects, both positively and negatively, the production of over 100 membrane proteins in Y. enterocolitica strain Ye9 of bio-serotype 2/O:9, a low pathogenicity strain that is not lethal for mice at low doses (Nieckarz et al., 2016). The loss of OmpR positively affected the production of heme receptor HemR1 in Y. enterocolitica cells grown in LB medium at 37 • C and preliminary data suggested indirect repression of hemR1 by OmpR.
In this study, we investigated the molecular mechanisms underlying OmpR-dependent production of two potential heme receptor proteins encoded within hemPRSTUV-1 (hem-1 locus) and hemPRST-2 (hem-2 locus) gene clusters identified in Y. enterocolitica strain Ye9. Firstly, we demonstrated that strain Ye9 utilizes heme-containing molecules as an iron source. The Y. enterocolitica hemin uptake system involved in transporting the entire heme moiety into the cytoplasm was described previously in strain WA-C, a mouse-virulent strain of bio-serotype 1B/O:8 Hantke, 1992, 1994). In contrast to strain Ye9, this strain is able to produce and utilize the siderophore yersiniabactin (Ybt), encoded on a chromosomal HPI (36 kb) Rakin et al., 1994Rakin et al., , 2012Pelludat et al., 1998).
In this study, two multigenic clusters, hemPRSTUV-1 and hemPRST-2 were identified in the genome of Ye9. The genetic organization of these clusters is identical except that the hem-2 locus lacks two ORFs, hemU2 and hemV2, encoding potential components of the heme transport system. Evidence from sqRT-PCR analysis revealed that both the hem-1 and hem-2 clusters are organized as operons, and expressed from the promoters p hem−1 and p hem−2 located upstream of the respective hemP genes. Experiments with a Y. enterocolitica strain carrying a plasmid-located p hem−1 ::lacZ reporter fusion showed that p hem−1 promoter activity is inhibited by iron, which suggested the involvement of Fe 2+ -Fur in this repression. It has been previously demonstrated in diverse microorganisms that Fur, activated upon binding ferrous iron, binds to specific genes to inhibit Boxes indicate the location of the FourU motif (shaded) and putative RBS, the translation start codon is marked red, and unpaired nucleotides within the predicted secondary structure are in green. (B) Temperature-dependent HemR1 expression in Y. enterocolitica examined by immunoblotting. The analyzed samples were cell lysates of the fur mutant grown at 26 or 37 • C in LB medium. The top panel shows the Western blot probed with a polyclonal antibody against HemR1, the bottom panel shows the Coomassie blue-stained gel as a loading control. The percentage values on the blot, indicating the HemR1 band intensities relative to that of cells grown at 26 • C, were determined using Amersham Imager 600 Analysis Software V1.0.0 (GE Healthcare). (C) Expression of a hemR1 ′ -′ gfp translational fusion at different temperatures, monitored by fluorescence intensity. E. coli BW25113 harboring the reporter plasmid pFX-P lac -hemR1 or control plasmids pFX-0 and pFX-1 were grown in LB medium at 26 or 37 • C. The GFP fluorescence intensity (RFU) of overnight cultures was determined. The data represent the averages ± SD from at least three experiments with duplicate cultures. Significance was calculated using one-way ANOVA [P > 0.05, *P < 0.05, ***P < 0.001]. (D) GFP abundance examined by immunoblotting. The analyzed samples were cell lysates of E. coli BW25113 harboring the plasmids pFX-0, pFX-1 or pFX-P lac -hemR1. The top panel shows the immunoblot probed with antibody against GFP, the bottom panel shows the TGX Stain-Free gel as a loading control. MW, 3-Color Prestained Protein Marker; kDa. These results are representative of at least three independent experiments. their transcription (Bagg and Neilands, 1987;de Lorenzo et al., 1987;Andrews et al., 2003). Based on experimental and/or bioinformatic analyses, almost all iron-acquisition systems of pathogenic yersiniae, i.e., Y. pestis, have been shown to be Furcontrolled (Gao et al., 2008). More importantly, the results we obtained using transcriptional fusions with the lacZ gene and by Western blot analysis demonstrated that OmpR negatively regulates p hem−1 activity and thus HemR1 protein expression under conditions of iron starvation. The precise role of OmpR in the repression of HemR1 expression was revealed in a fur mutant background. While the ompR and fur mutations alone led to an increase in HemR1 synthesis, the combination of the two produced an additive effect. These data showed that OmpR inhibits HemR1 production in the absence of Fur. However, the OmpR-dependent regulation of HemR1 expression seems to be much more complex, since the effect of OmpR was also observed at the transcriptional and HemR1 protein level in iron-replete conditions, when the Fur repressor is active.
EMSA analysis revealed that OmpR acts directly at the p hem−1 promoter. We observed that OmpR specifically recognizes and binds a p hem−1 promoter fragment containing the in silicopredicted OmpR-binding sequence. This result confirmed a direct role for OmpR in mediating hemR1 repression. In the hem-1 promoter, the predicted 19-bp Fur box (FSB-1) overlaps the −10 promoter motif, and is only 10 bp downstream of the 20-bp putative OmpR-binding site (OBS-1) at the −35 motif. Based on the location of these binding sites, we propose the following model for Fur-and OmpR-dependent repression of hem-1. Consistent with the notion that under iron-starved conditions Fur-dependent repression is abolished, OmpR may interact with OBS-1 and prevent the polymerase binding to the promoter, leading to inhibition of hem-1 expression. In iron-replete medium, there are a number of possible ways in which OmpR could regulate hem-1 expression. It is likely that binding of OmpR to the p hem−1 promoter leads to a DNA structure that is favorable for the function of Fe 2+ -Fur. Recent reports have suggested that the OmpR of Salmonella enterica may influence DNA topology to control target promoter activity (Cameron and Dorman, 2012;Quinn et al., 2014). We do not rule out the possibility that OmpR-Fur protein interactions could stimulate the binding of Fur to the hem-1 promoter, which would lead to inhibition of transcription initiation. Further research is required to characterize the molecular mechanisms modulating the activity of Fur at the hem-1 promoter in the presence of OmpR.
Similarly to the hem-1 locus, the hem-2 gene cluster hemPRST-2 is organized as an operon. However, p hem−2 promoter activity was much lower than p hem−1 and iron repression was detected only at 26 • C. Although a sequence displaying 74% identity to the Fur box consensus was recognized in p hem−2 (at the −10 motif), the potential regulatory role of Fe 2+ -Fur might be dependent on other thermoregulated factors. The role of OmpR in the activity of p hemR2 was studied by analyzing the expression of a p hem−2 ::lacZ fusion in the wild-type strain with a normal or raised OmpR content. This experiment revealed that OmpR negatively regulates hem-2 expression. Interestingly, in the presence of an increased level of OmpR, iron regulation was abolished. Moreover, in EMSAs, we were able to detect the specific interaction of OmpR with the putative OmpR binding site in p hem−2 , but with lower affinity than with OBS-1 in the hem-1 promoter. The higher binding sequence degeneracy in p hem−2 (45% identity to the consensus sequence for OBS-2 vs. 60% identity for OBS-1) might explain the weaker interaction of OmpR with this promoter.
Taken together, these results demonstrated the importance of the OmpR regulator in hem-1 and hem-2 expression, and hence the production of the HemR1 and HemR2 heme receptors in Y. enterocolitica. Our findings suggest that the effects of OmpR on hem-1 and hem-2 transcription are likely to be direct, i.e., produced by binding to specific DNA sequence elements in the promoter regions. The negative effect of OmpR on hem-1 transcription occurs in both the absence and presence of the Fur repressor. The role of OmpR in regulating hem-2 expression is difficult to assess and needs further study.
Thermo-induced structural changes in mRNA play a fundamental role in temperature sensing in bacteria and influence virulence gene expression in many pathogens, including those of the genus Yersinia (Narberhaus et al., 2006;Narberhaus, 2010;Böhme et al., 2012). Analysis of the intergenic region of the hemP1-hemR1 transcript in locus hem-1, but not in locus hem-2, in Y. enterocolitica Ye9, suggested the formation of a secondary structure in the hemR1 5 ′ -UTR with a FourU element that may sequester the ribosome binding site. Such an RNA structure was previously shown to be sufficiently stable at moderate temperatures (25 • C) to inhibit expression of the ShuA receptor in S. dysenteriae. Melting of the FourU motif at 37 • C permits access of ribosomes and initiates shuA translation (Kouse et al., 2013). However, Western blot analysis revealed that HemR1 of Y. enterocolitica is not subject to thermoregulation. Despite the presence of a FourU sequence in the 5 ′ -UTR of hemR1, the unpaired G-G sequence within the hairpin may destabilize this secondary structure. In S. dysenteriae, only a single nucleotide replacement within the shuA hairpin led to destabilization of this inhibitory structure and resulted in increased expression of this gene at the non-permissive temperature of 25 • C (Kouse et al., 2013). A stretch of four uracils located within an intergenic region of the yscW-lcrF transcript in a pathogenic Yersinia has been linked with the thermally regulated expression of the transcript encoding LcrF, the transcriptional activator of yop and ysc genes of the type III secretion system (Böhme et al., 2012). It is notable that analyses of the expression of a hemR1 ′ -′ gfp translational fusion and the level of a HemR1 ′ -′ GFP fusion protein in E. coli showed a degree of temperature dependence, suggesting that a mechanism of thermoregulation, unconnected with the presence of a FourU RNA thermometer, is active in this heterologous genetic background.
HemR1 and the newly identified HemR2 protein exhibit a high degree of amino acid sequence similarity (62% identity), including the presence of signature motifs like the TonB box, the conserved V region associated with all TonB-dependent OM proteins, and histidine residues required for heme transport via the receptor channel (Kadner, 1990;Bracken et al., 1999).
Significant similarities between HemR1/HemR2 and heminbinding proteins suggest that these two Y. enterocolitica proteins are involved in the binding and utilization of hemin and hemeproteins. The expression of the HemR1 or HemR2 protein from their native promoters in E. coli SASX77, a hemA mutant defective in the biosynthesis of a heme precursor and naturally lacking a heme binding protein in the outer membrane Mills and Payne, 1995), permitted hemin and hemoglobin utilization. The strain expressing HemR2 was less efficient in utilizing hemin or hemoglobin, which might reflect lower activity of the p hem−2 promoter. It is also possible that structural or functional differences in these OM receptors may influence the transport of the heme moiety.
Taken together the findings of this study indicate that Y. enterocolitica HemR1 and HemR2 are outer membranereceptors that play an important role in hemin and hemoglobin utilization. We hypothesize that HemR1 and HemR2 together with HemTUV-1, a periplasmic/inner membrane ABC heme transporter also encoded by the hem-1 locus, may constitute a system involved in the acquisition of the heme moiety from host hemoproteins under the varied conditions encountered by Y. enterocolitica during an infection. It is also possible that HemR2 together with an as yet unidentified cytoplasmic transporter constitute an alternative system for the utilization of heme and host hemoproteins. Such a system might be able to counteract the loss/inactivation of the Y. enterocolitica heme uptake system based on the HemR receptor. Pathogenic bacteria frequently possess more than one transport system for iron or heme/hemoprotein uptake (Braun et al., 1998), so additional systems besides those involving the TonB-dependent HemR1 and HemR2 receptors may help perform this vital function in Y. enterocolitica.
In conclusion, this study has demonstrated that the regulators Fur and OmpR participate in a complex mechanism governing the negative regulation of Y. enterocolitica hemR1 and hemR2. This interplay might be responsible for fine-tuning the expression of HemR receptor proteins mediating iron/heme acquisition during infection, to permit rapid growth while avoiding toxicity.

AUTHOR CONTRIBUTIONS
KJ, AR, and KB designed the study. KJ, MN, and ML performed the experiments. KB, AR, KJ, and ML analyzed the data. AR, KB, and KJ wrote the manuscript. KJ and MN designed and prepared the figures. KB provided financial support.

FUNDING
This work was supported by a grant from the National Science Center, Poland (OPUS grant UMO-2016/21/B/N26/011009). Publication co-financed by the University of Warsaw.