yqhG Contributes to Oxidative Stress Resistance and Virulence of Uropathogenic Escherichia coli and Identification of Other Genes Altering Expression of Type 1 Fimbriae

Urinary tract infections (UTIs) are common bacterial infections and the vast majority of UTIs are caused by extraintestinal pathogenic Escherichia coli (ExPEC) strains referred to as uropathogenic E. coli (UPEC). Successful colonization of the human urinary tract by UPEC is mediated by secreted or surface exposed virulence factors—toxins, iron transport systems, and adhesins, such as type 1 fimbriae (pili). To identify factors involved in the expression of type 1 fimbriae, we constructed a chromosomal transcriptional reporter consisting of lux under the control of the fimbrial promoter region, fimS and this construct was inserted into the reference UPEC strain CFT073 genome at the attTn7 site. This fimS reporter strain was used to generate a Tn10 transposon mutant library, coupled with high-throughput sequencing to identify genes that affect the expression of type 1 fimbriae. Transposon insertion sites were linked to genes involved in protein fate and synthesis, energy metabolism, adherence, transcriptional regulation, and transport. We showed that YqhG, a predicted periplasmic protein, is one of the important mediators that contribute to the decreased expression of type 1 fimbriae in UPEC strain CFT073. The ΔyqhG mutant had reduced expression of type 1 fimbriae and a decreased capacity to colonize the murine urinary tract. Reduced expression of type 1 fimbriae correlated with an increased bias for orientation of the fim switch in the OFF position. Interestingly, the ΔyqhG mutant was more motile than the WT strain and was also significantly more sensitive to hydrogen peroxide. Taken together, loss of yqhG may decrease virulence in the urinary tract due to a decrease in production of type 1 fimbriae and a greater sensitivity to oxidative stress.


INTRODUCTION
Urinary tract infections (UTIs) can occur throughout the urinary tract within the urethra, bladder, ureters, or kidneys. UTIs are a common infectious disease with over 150 million cases documented worldwide each year (Stamm and Norrby, 2001;Foxman, 2014). Furthermore, the overwhelming majority of uncomplicated UTI cases (≥80%) are caused by extraintestinal pathogenic Escherichia coli (ExPEC), referred to as uropathogenic E. coli (UPEC) (Ronald, 2002), which can belong to a diversity of phylogenetic groups or sequence types (Russo and Johnson, 2000;Chen et al., 2013). An estimated 50% of women experience at least one UTI during their life, and up to a quarter of those women are prone to recurrent UTIs (Foxman, 2002). Adherence of UPEC to host cells is a key event in initiating UTI pathogenesis and is important for overcoming strong urine flow, and to promote urinary tract colonization (Ulett et al., 2013;Flores-Mireles et al., 2015).
Fimbriae (pili) are filamentous structures that can mediate adherence of bacteria to host cell receptors (Mulvey et al., 1998;Nielubowicz and Mobley, 2010). For instance, type 1 fimbriae are critical for colonization of the bladder by UPEC (Gunther et al., 2002), to directly stimulate UPEC invasion into epithelial cells and aid in formation of intracellular reservoirs that may contribute to recurrent infection (Mysorekar and Hultgren, 2006). Type 1 fimbriae encoded by the fimAICDFGH (fim) genes, are one of the best-characterized UPEC chaperoneusher fimbriae. These fimbriae are produced by most E. coli strains including UPEC (Buchanan et al., 1985;Sivick and Mobley, 2010). The fimA gene encodes the type 1 major fimbrial subunit and the tip-located adhesin, encoded by fimH, mediates binding to α-d-mannosylated receptors, such as uroplakins, which are abundant in the bladder and other host surfaces containing mannosides (Connell et al., 1996;Wu et al., 1996). The fim promoter is located on a 314-bp invertible DNA promoter element (fimS), the orientation of which determines the transcriptional status (ON or OFF) (Abraham et al., 1985;Bjarke Olsen and Klemm, 1994). The switching orientation of fimS is controlled by two recombinases. FimB, which promotes inversion in both orientations, and FimE, which mediates the switching from phase-on to phase-off (Klemm, 1986;Gally et al., 1996). Two additional recombinases, IpuA and IpbA, present in UPEC strain CFT073 can also mediate switching of the invertible element independent of FimB and FimE (Bryan et al., 2006). Regulation of fim genes is affected by multiple environmental factors (including pH, osmolarity, temperature and oxygen levels) and at least three regulatory proteins are directly implicated (Lrp, IHF, and H-NS) (Unden and Kleefeld, 2004).
To establish a UTI, UPEC strains must resist environmental stresses in the bladder and kidneys including pH stress and wide fluctuations in osmolarity (Culham et al., 2001;Cahill et al., 2003). The high osmolality, high urea concentration, acidic pH and organic acids in urine can limit the growth and survival of E. coli within the urinary tract . Thus, an important aspect of UPEC virulence is the capacity to resist high osmolality and the denaturing effects of urea, and rapidly adapt to changes and stress encountered in these niches during establishment of the infection.
Previously, we showed that interference with phosphate homeostasis decreased the expression of type 1 fimbriae in strain CFT073 and attenuated UPEC virulence (Crépin et al., 2012b). The phosphate-specific transport (Pst) system negatively regulates the activity of the two-component signal transduction system PhoBR and also transports inorganic phosphate. Inactivation of the Pst system results in constitutive activation of PhoBR regardless of environmental phosphate availability (Wanner, 1996). As such, a pst mutant responds as if it is always under phosphate-limiting conditions. In UPEC, we showed that decreased virulence of a pst mutant is largely due to reduced expression of type 1 fimbriae. In avian pathogenic E. coli (APEC), an altered membrane homeostasis was also observed in a pst mutant (Lamarche and Harel, 2010) and caused increased sensitivity to acid, cationic antimicrobial peptides, and serum (Lamarche et al., 2005;Crépin et al., 2008;Bertrand et al., 2010).
In order to identify genes affecting the expression of type 1 fimbriae, we constructed a transcriptional luciferase (lux) reporter consisting of lux under the control of the fimS invertible promoter and this construct was inserted into the CFT073 genome at the attTn7 site. This fimS reporter containing strain was used to generate a Tn10 transposon mutant library, coupled with high-throughput sequencing to identify the location of transposon insertions that altered the expression of type 1 fimbriae. In this report, we show that YqhG is one of the important mediators that contribute to decreased expression of type 1 fimbriae in UPEC strain CFT073. Our results demonstrated that the deletion of yqhG in CFT073 reduced the expression of type 1 fimbriae and reduced urinary tract colonization of the yqhG mutant in the murine model. We also examined whether deletion of yqhG as well as the pst system also reduced resistance to environmental stresses, suggesting that altered expression of type 1 fimbriae can be linked to changes in bacterial adaptation to environmental stresses, such as oxidative or osmotic stress.

Bacterial Strains, Growth Conditions, and Plasmids
E. coli strains and plasmids used in this study are listed in Table 1. E. coli CFT073 was isolated from the blood and urine of a patient with acute pyelonephritis (Mobley et al., 1990). Bacteria were routinely grown in lysogeny broth (LB) (Alpha Bioscience, Baltimore, MD) at 37 • C and in human urine. Urine was obtained from healthy female volunteers, 20-40 years old, with no occurrence of a UTI or antibiotic use within the last 2 months prior to collection. A protocol for obtaining biological samples from human donors was reviewed and approved by the ethics committee-Comité d'éthique en recherche  of INRS. Urine was immediately filter sterilized (0.2-µm pore size), pooled, and frozen at −80 • C and used within 2 weeks of sampling. Antibiotics and reagents when required were added at the following concentrations: kanamycin, 50 µg/ml; ampicillin, 100 µg/ml; chloramphenicol 30 µg/ml and diaminopimelic acid (DAP), 50 µg/ml.  Herrero et al., 1990 (luxCDABE) (Allen, 2000) was amplified with the primers CMD1733 and CMD1734 (Supplemental Table 1). This DNA fragment was digested with KpnI and ApaI (New England Biolabs), purified with the Biobasic kit and ligated into the multiple-cloning sites (MCS) of the mini-Tn7-containing vector pGP-Tn7-Cm, generating the vector pGP-Tn7-lux (pIJ461). An optimized ribosome-binding site, RBS, was then added to plasmid pIJ461 to generate the pIJ514 vector. Then, the plasmid pIJ514 was used to generate the vectors, pIJ516 pfimA phase L-ON, pIJ517 pfimA phase L-OFF and pIJ518 pfimA variable phase. The fim promoter from strain CFT073 was amplified by PCR with primers CMD1645 and CMD1646 (see Supplemental Table 1). Genomic DNA, digested with the restriction enzymes EcoRI and SalI, and ligated to pIJ514 previously digested with EcoRI and XhoI. Transformation into E. coli DH5α cells was followed by selection on LB plates containing chloramphenicol. Using the approach described by Gunther et al. (2002), point mutations in primer CMD1133 were introduced to block the promoter switch in the ON position, digested with the restriction enzymes SmaI and SalI, and ligated to pIJ514 plasmid digested with SmaI and XhoI. The resulting vectors pIJ516, pIJ517, pIJ518 were transformed in E. coli SM10 λpir-derivative strain MGN-617. Strain MGN-617 (pGP-Tn7-fimS-lux) was conjugated overnight with strain CFT073, containing plasmid pSTNSK, which encodes the Tn7 tnsABCD transposase genes, at 30 • C on LB agar plates supplemented with DAP. Following overnight culture, the bacteria from agar plates were suspended in 1 ml of phosphate-buffered saline (PBS), washed twice in PBS, serially diluted, and cultured on LB agar supplemented with gentamicin, and incubated at 37 • C. Colonies that grew were then tested for sensitivity to kanamycin and ampicillin, indicating the likelihood of integration at attTn7 and loss of the transposaseencoding plasmid pSTNSK. Insertion of Tn7 into the attTn7 site was verified by PCR (primers CMD26 and CMD1416 (see Supplemental Table 1).

Testing the fim-lux Fusions Under Different pH Conditions
To measure the changes following growth in media at different pH, LB was buffered using 0.1 M Na 2 HPO 4 -NaH 2 PO 4 buffer. The media were prepared with a pH ranging between 4.4 and 7.0. E. coli containing pfimA phase-variable, pfimA L-ON and pfimA-L-OFF lux fusions were incubated overnight at 37 • C, 250 rpm in 5 ml LB medium. The next day 3 µl of each overnight culture was transferred to 180 µL of buffered LB at a specific pH and incubated with agitation until midlogarithmic phase had been reached in 96-well plates at 37 • C. The luminescence and O.D. 600nm was measured each 15 min for 4 h using a Cytation TM 3 Cell Imaging Multi-Mode Reader (BioTek Instruments Inc.). The luminescence results were reported as relative luminescence units (RLU). The luminescence readings were normalized to the O.D. 600nm values. The luminescence of lux fusion-containing strains streaked on LB agar plates was recorded with a ChemiDoc XRS system equipped with Quantity One 1-D analysis software (Syngene Chemi Genius), with an integration time of 30 s.

Transposon Mutagenesis
Transposon mutagenesis was performed as described by Simms and Mobley (2008). Briefly, the MGN-617/pLOF-Km donor strain and recipient strain CFT073 carrying fimS phase variable reporter were cultured overnight (O/N) at 37 • C in LB with appropriate antibiotics and supplements. Mixed cultures were prepared as a 1:4 donor-to-recipient ratio, placed onto LB agar plates supplemented with IPTG and DAP, and incubated O/N at 37 • C. After incubation, cells were suspended in 1 ml of PBS, washed twice in PBS, serially diluted, and plated onto LB agar supplemented with kanamycin and incubated O/N at 37 • C to select for the recovery of kanamycin-resistant transposon mutants of the CFT073 fimS-lux recipient strains. Colonies were screened for susceptibility to ampicillin to confirm loss of pLOF-Km.

PfimA-lux
Transposon mutants of CFT073 carrying fimS-lux were cultured at 250 rpm in 150 µL of LB in a 96-well plate (Corning White With Clear Flat Bottom), and luminescence was measured at O.D. 600nm . In total, 5,904 transformants were analyzed. The luminescence readings were normalized to the O.D. 600 values to account for any differences in growth. Mutants with disrupted genes that resulted in higher or lower levels of luminescence than the WT pfim-lux-fusion containing strain, were confirmed phenotypically by quantification of type 1 fimbriae by yeast agglutination assays as described below. Mutants of interest were then streaked on LB agar over three successive rounds of subculture and then stored individually in 25% glycerol at −80 • C.

Evaluation of Type 1 Fimbriae Production
The production of type 1 fimbriae was determined by yeast agglutination assay . Briefly, the transposon mutants were cultured at 37 • C in LB broth or human urine to mid-log phase. In our experiment, log-phase (period of steadystate growth in LB) is estimated to occur at OD 600 (optical density at 600 nm) between 0.6 and 0.8 However, for growth in urine, cells reach a stationary phase at an OD 600 of 0.5-0.9. As such, we used a growth of cells to an OD 600 of 0.6 for cells for mid-log growth in LB and an OD 600 of 0.4 for mid-log growth in human urine. Following centrifugation, 40 µl of an initial suspension of ∼2 × 10 11 cells ml −1 in PBS was transferred and serially diluted 2-fold in microtiter wells containing equal volumes of a 3% commercial yeast suspension in PBS. After 30 min of incubation on ice, yeast aggregation was monitored visually, and the agglutination titer was recorded as the most diluted bacterial sample giving a positive aggregation reaction.

Site-Specific Integration of Tn10
Genomic DNA of 32 clones was extracted from cultures using phenol-chloroform. DNA was sequenced at the Génome Québec Innovation Centre, McGill University. DNA concentrations were determined using the Quant-iT TM PicoGreen R dsDNA Assay Kit (Life Technologies). DNA samples were generated using the NEB Next Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) as per the manufacture protocol. TruSeq adapters and PCR primers were purchased from IDT. Size selection of libraries containing the desired insert size was obtained using SPRI select beads (Beckman Coulter). Briefly, genomic DNA was fragmented and tagged with adapter sequence via one enzymatic reaction (tagmentation). We initially amplified by PCR the region between the end of the insertion (primer Tn_pLOF-Km-CS1:(5 ′ ACACT GACGACATGGTTCTACAcgttgcgctgcccggattac 3 ′ [transposonspecific sequence is in lowercase])), and the Illumina adapter with primer 2 (5 ′ TACGGTAGCAGAGACTTGGTCTCTAG-CATAGAGTGCGTAGCTCTGCT 3 ′ ) to enrich for transposon insertion sites and allow multiplex sequencing. The thermocycler program was 94 • C for 2 min, 94 • C for 30 s, 55 • C for 30 s 72 • C for 30 s for 33 cycles and 72 • C for 7 min. Each library was prepared with a unique Illumina barcode. We amplified this region to add the Illumina adapters for MiSeq sequencing: PE1-CS1 (AATGATACGGCGACCACCGA-GATCTACACTGACGACATGGTTCTACA) and primer 2. The libraries were then pooled in equimolar concentration and sequencing was performed on an Illumina MiSeq using the MiSeq Reagent Kit v2 Kit (500-cycles).
After determining the location of the transposon, the clones in the pool carrying the specific mutations were determined using a primer complementary to the transposon end and another primer complementary to the identified transposon-interrupted gene. Following DNA amplification of each clone by PCR, we were able to determine which specific clones contained some of the identified site-specific insertions.

Construction of Site-Directed Mutants and Complementation of Strains
Mutations were introduced by lambda-red recombination as described using plasmids pKD3 and pKD4 as templates for chloramphenicol and kanamycin resistance cassettes, respectively (Datsenko and Wanner, 2000). Primers are listed in Supplemental Table 1. Antibiotic cassettes flanked by FLP recombination target (FRT) sites were excised by introduction of vector pCP20 expressing the FLP recombinase (Cherepanov and Wackernagel, 1995).

Preparation of Fimbrial Extracts and Western Blotting
Preparation of fimbrial extracts and western blotting were performed as described previously , with antitype 1 fimbriae serum from E. coli strain B AM . Briefly, after the growth to log phase, the bacteria were harvested and resuspended in 5 ml of 150 mM NaCl-0.5 mM Tris-HCl (pH 7.8). Following incubation at 56 • c for 1 h and centrifugation (3,000 × g for 10 min), the aliquot was precipitated with 10% trichloroacetic acid. Followed by centrifugation 20,000 × g for 15 min at 4 • C, the pellet was washed twice with 0.5 M Tris-HCl-0.5 M EDTA (pH 12.0) and resuspended in 0.5 M Tris-EDTA.
Further, fimbrial extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the proteins were stained with Coomassie brilliant blue and transferred to a nitrocellulose membranes (Bio-Rad) for 60 min at 100 V. The membrane was blocked with supplemented with 0.05% Tween 20 (Pierce). Incubations with primary rabbit anti-fim (1:5,000) and secondary goat anti-rabbit (1:25,000) antibodies were carried out for 1 h at room temperature. SuperSignal West Pico chemiluminescent substrate (Pierce) was used for detection.

Detection and Quantification of the On/Off State of the fimS Region
The orientation of the fimS region was determined as described previously (Müller et al., 2009). Briefly, the fimS region was PCR amplified with the primers CMD1258 and CMD1259 (see Supplemental Table 1), to produce a 650 bp fragment. The DNA was then digested with HinfI and analyzed on a 2% agarose gel. Following digestion, the ON orientation produces fragments of 128 and 522 bp, whereas the OFF orientation generates fragments of 411 and 239 bp. Quantification of the ratio of cells in ON or OFF position was performed as described (Wu and Outten, 2009). The WT strain was cultured statically for 48 h at 37 • C for use as a control for increased orientation of the ON position. The WT strain was also cultured for 24 h on LB agar plates at room temperature as a control to favor orientation in the OFF position.

Experimental UTI in CBA/J Mice
The animal study was reviewed and approved by the animal ethics evaluation committee-Comité Institutionel de Protection des Animaux (CIPA No 1608-02) of INRS. The murine experimental UTIs were carried out as described previously (Hagberg et al., 1983), using a single-strain infection model. Prior to inoculation, strains were grown for 16 h at 37 • C with shaking (250 rpm) in 55 ml of LB medium. Six-weeks-old CBA/J female mice were inoculated through a catheter inserted in the urethra with 20 µl of the pellet containing 2 × 10 9 CFU of either UPEC strain CFT073, CFT073 yqhG (QT5178) or the complemented strain (QT5235). After 48 h, mice were euthanized; kidneys and bladders were sampled, homogenized, diluted, and plated on MacConkey agar for enumeration of colonies.
Adhesion Assays 5,637 human bladder cells (ATCC HTB-9) were grown in RPMI 1640 medium (Wisent Bioproducts) supplemented with 10% fetal bovine serum, 2 mM l-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/liter glucose, and 1.5 g/liter sodium bicarbonate. For the assays 5,637 cells were grown to confluency in RPMI 1640 and 2 × 10 5 cells/well were distributed in 24well plates. Strain CFT073 and isogenic mutants were grown in LB medium at 37 • C to the mid-log phase of growth (O.D. 0.6). Immediately before infection, cultures were washed with PBS, suspended in medium and inoculated at a multiplicity of infection (MOI) of 10 CFU per epithelial cell. Bacteria-epithelial cell contact was enhanced by a centrifugation at 600 × g for 5 min. After 2 h, cells were washed three times and lysed with PBS−0.1% sodium deoxycholate (DOC), serially diluted, and plated on LB agar plates. Quantification of cell-associated bacteria was performed as previously described . To block adherence mediated by type 1 fimbriae, 2.5% α-dmannopyranose was added.

Motility Assay
Motility assays were as previously described (Lane et al., 2005) with modification. Following overnight growth at 37 • C, strains were cultured at 37 • C in LB broth to mid-log phase. Strains were stabbed into the surface of soft agar (1% tryptone, 0.5% NaCl, 0.25% agar) using an inoculating needle. Care was taken not to touch the bottom of the plate during inoculation to ensure only swimming motility was assessed. After 16 h of incubation, the diameters of motility zones were measured. Three independent motility experiments for each strain were performed. Results were analyzed using a paired t-test.

Growth Under Conditions of Osmotic Stress
Strains were tested for growth under conditions of osmotic stress using NaCl or urea. Cultures were diluted 1:100 from overnight cultures grown in LB, grown until mid-log phase with shaking. They were serially diluted and plated on LB agar alone and LB agar supplemented with 0.3 M NaCl, 0.6 M NaCl, 0.3 M urea, or 0.6 M urea. Colonies were enumerated, and growth under each condition was compared to growth on LB agar.

Hydrogen Peroxide Sensitivity Assay
Sensitivity to H 2 O 2 was determined by using an agar overlay diffusion method (Boyer et al., 2002). Briefly, overnight cultures were used to inoculate (1/100) fresh LB medium, and incubated until the O.D. 600 reached 0.6. Then, 100 µl of each culture were mixed with 3 ml molten top agar and poured onto an LB agar plate. A 6-mm-diameter Whatman filter disk containing 10 µl 30% H 2 O 2 was placed on the agar surface and plates were incubated overnight at 37 • C. Inhibition zone diameters were then measured.

Statistical Analyses
Statistical tests were obtained using the Prism 7.04 software package (GraphPad Software). Statistically significance between two groups was determined by unpaired t-test and comparison among three or more groups was obtained by one-way analysis of variance (ANOVA). For the independent infections, comparisons of the CFU mL −1 or CFU g −1 distributions were analyzed using the Mann-Whitney test.

RESULTS
The Single-Copy Integrated CFT073

fimS-lux Reporter System
To identify systems that alter the expression of type 1 fimbriae, we used a promoterless lux reporter system fused to the fim type 1 fimbriae promoter region, fimS. A luxCDABE reporter system originally from Photorhabdus luminescens (Allen, 2000) was used. The system encodes all the enzymes needed to produce a luminescent signal. The lux genes were introduced into the pGP-Tn7-Cm vector. An optimized ribosome binding site, RBS, was added to plasmid pIJ461 to generate pIJ514 vector. Further, we generated phase variable, pfimA phase variable-lux (pIJ518) to measure the expression of type 1 fimbriae in various conditions ( Figure 1A). Using the same approach as described by Gunther et al. (2002), point mutations were introduced in order to lock the promoter in the ON-and OFF-position, respectively to generate pfimA L-ON-lux (pIJ516) and pfimA L-OFF-lux (pIJ517) (Figure 1).

Analysis of the fim-lux Fusions in UPEC CFT073 Grown Under Different pH Conditions
To better characterize the reporter system upon dynamic transcriptional changes, we set out to assay the response kinetics of the pfimA promoter, driving the expression of the type 1 fimbriae using the lux reporter system. This promoter has been extensively studied in E. coli and used to validate a lux reporter system and lacZYA fusion implemented on a single copy number plasmid (Schwan et al., 2002;Schwan and Ding, 2017). Thus, it has been reported that growth conditions play a substantial role in the ability of E. coli cells to undergo phase variation and alter expression of type 1 fimbriae. Of note, transcription of all of the fim genes was shown to be repressed in a low pH environment (Schwan et al., 2002). Following a similar protocol, the fim-lux reporter containing variant of CFT073 or its isogenic pst mutant was grown in LB adjusted at different pH conditions ranging from 4.4 to 7. To verify any differences in expression, the E. coli cells were grown to mid-logarithmic phase (OD 600 of 0.6). The fimS-Locked ON fusion had the highest expression level and did not vary regardless of the pH during growth (Figure 2A). However, the neutral pH influenced the expression of the fim switch. A shift from pH 4.4 to a neutral pH of 7 in LB media resulted in a 2-fold increase in expression of the fimS promoter. Decrease in pH also diminished the production of type 1 fimbriae (Supplemental Figure 1). In addition, no significant change in growth rates was observed for growth of the CFT073 parent strain and the PfimA-Locked ON and PfimA variable lux derivatives, suggesting that the expression of the lux operon and its gene products had no adverse effects on bacterial growth (Supplemental Figure 2).
CFT073 derivatives carrying the PfimA promoter in either direct (PfimA-Locked ON) or opposite (PfimA-Locked OFF) orientation with respect to the luxCDABE operon were streaked on LB agar plates, together with the CFT073 strain and a pst mutant carrying the PfimA phase-variable lux fusion. Stronger emission was detected from the fimS-Locked ON fusion ( Figure 2B), with single colonies producing a strong signal. By contrast, there was no luminescence signal detected in the negative control carrying the PfimA-Locked-OFF fusion or the CFT073 strain or its pst mutant carrying the PfimA-phasevariable-lux fusion, although a dim light emission could be detected for CFT073 containing the PfimA-phase-variable fusion after longer exposure. In addition, the stability of the fimS-Locked-ON-lux strain was evaluated after 10 passages without antibiotic selection and the maintenance and luminescence expression of this fusion was found to be stable.
The expression of lux derivatives was also compared in LB liquid media overnight. As expected, there was a high signal for the fimS-Locked ON strain (QT4794), no signal for the fimS-Locked OFF strain (QT4795) and an intermediate signal for the fimS-lux variable strain (QT4796) (Figure 2C). In contrast, the signal for pst, fimS-phase variable-lux (QT5018) increased significantly ( Figure 2C) when compared with the same mutant at OD 600 0.6 (Figure 2A). This result was similar to what was previously observed (Supplemental Figure 1) (Crépin et al., 2012b).

Screening for Transposon Mutants With Increased or Decreased lux Expression and Altered Production of Type 1 Fimbriae
To identify genes affecting the expression of type 1 fimbriae, E. coli CFT073 carrying the fimS phase-variable reporter ( Figure 1B) was subjected to transposon mutagenesis. A mini-Tn10 transposon carrying a kanamycin resistance marker pLOF/Km  was randomly inserted into the bacterial chromosome. A transposon bank was screened for luminescence. Clones demonstrating increased or decreased light emission were identified. Transposon insertions that produced high or low levels of luminescence may identify genes encoding transcriptional repressors or activators of fim. Transposon insertions that result in low levels of luminescence suggest that the disrupted genes may promote fim transcription and production of type 1 fimbriae. A total of 5,904 transposon mutants were generated and stored individually in 96-well plates stored at −80 • C. The mutants were assessed for luminescence at O.D. 600nm 0.6 with shaking at 37 • C ( Figure 3A). The mobilizable suicide vector pGP-Tn7-Cm-fimS-lux contains the conjugative transfer Mob RP4 and the ori R6K. A multiple cloning site is integrated between the two Tn7 ends, where the promoterless lux reporter system is fused to the fimbrial promoter region, fimS. (B) Tn7-based transposition at the chromosomal attTn7 site was achieved by conjugation, where the donor strain (E. coli SM10λpir) harbored the mini-Tn7 vector and the recipient strain CFT073 contained the thermosensitive suicide vector pSTNSK pSC101-temperature sensitive origin and transposases tnsABCD. The Tn7 transposon integrates at the site-specific attTn7, located downstream of the highly conserved glmS gene.
FIGURE 2 | Response of the lux reporter system in exponential-phase, overnight cultures and on agar. (A) Effects of pH on fimS-phase variable, fimS-Locked ON and fimS-Locked OFF expression was determined with luxABCDE transcriptional fusions in strain CFT073. Luminescence was normalized to the O.D. 600nm of the culture compared to CFT073; means ± standard deviations are indicated from at least three separate runs. (B) Inverted darkfield image of luminescence emitted on plates by CFT073 and pst reporter strains carrying PfimA from the constitutively expressed promoter (fimS-LON) or inverted promoter (fimS-LOFF) or switch variable (fimS-phase variable) direction with respect to luxCDABE. Colonies were grown overnight at 37 • C and imaged using white light 400 ms (top right corner) or luminescence imaging for 30 s exposure time. Exposure images were acquired through ChemiDoc XRS system using high sensitivity chemiluminescence settings. (C) Response of the lux reporter system in overnight cultures in LB broth. Statistical significance was calculated by the Student t-test: *P < 0.05; **P < 0.005; ***P < 0.0001.
A subset of 65 transposon mutants demonstrating the highest and lowest levels of luminescence compared to the control CFT073 (attTn7 PfimA-lux) were further evaluated. Mutants that produced less luminescence than the control strain were further tested for levels of production of type 1 fimbriae by yeast agglutination assay (Crépin et al., 2012b). From this secondary screen, 48 transposon mutants were confirmed and there was at least a 4-fold increase or decrease in yeast agglutination (p<0.0001) (Figure 3B). Some of these mutants are currently being investigated to determine the transposon

Identification of Mutations Affecting Type 1 Fimbriae Expression
Analysis of the 32 mutants generated 6,733 sequence reads that mapped to the CFT073 genome. The insertions were linked to genes involved in protein fate and synthesis, energy metabolism, adherence, transcriptional regulation, and transport, regulatory functions, etc. (Table 2). A number of transposon mutations were also inserted in genes predicted to encode proteins of unknown function (Supplemental Figure 3).

Insertions Within Genes Contributing to Amino Acid Biosynthesis and Metabolism
Several clones carried an insertion within genes with metabolic functions. For instance, one of these clones was inserted in tdcB ( Table 2) that encodes a catabolic threonine dehydratase involved in the first step in threonine degradation. It is one of several enzymes carrying out the first step in the anaerobic breakdown of L-threonine to propionate (Umbarger and Brown, 1957). However, TdcB is activated by cAMP. Following a transition from aerobic to anaerobic growth, cAMP levels rise dramatically, which leads to increased expression of tdcB and consequently high levels of catabolic threonine deaminase (Hobert and Datta, 1983).
Similarly, another insertion was in nadB (Tritz et al., 1970) which is involved in NAD biosynthesis under both aerobic and anaerobic conditions (Messner and Imlay, 2002). Another mutant had an insertion in a pathogen-specific iron acquisition gene, iucC, which encodes a protein required for synthesis of aerobactin (de Lorenzo and Neilands, 1986). Likewise other insertion sites were yecK, encoding a membrane anchored pentaheme c-type cytochrome involved in an anaerobic respiratory system (Gon et al., 2000); eutE which codes for a protein that increases the level of acetylating acetaldehyde dehydrogenase activity (Rodriguez and Atsumi, 2012) and sseB involved in increased rhodanese activity (Hama et al., 1994).

Insertions Within Genes Encoding Other Fimbrial Adhesins, Transporters, or Outer Membrane Proteins in E. coli CFT073
A number of mutants with altered lux expression were found to contain insertions in genes encoding fimbriae, outer membrane proteins and transport proteins. Among these mutants, three carried insertions in fimbrial systems. One insertion was identified in fimD which is the usher of the chaperone-usher pathway of type 1 fimbriae (Supplemental Figure 3) (Klemm and Christiansen, 1990), the other mutant had an insertion in aufG which codes for a putative fimbrial-like adhesin protein, and another insertion was identified in c1933 (ydeS) encoding the minor fimbrial subunit of F9 fimbriae ( Table 2). These loci have been previously characterized Ulett et al., 2007). We also identified insertions in genes encoding a putative ABC transporter periplasmic binding protein, ydcS, a predicted outer membrane protein, yaiO, and an outer membrane porin, nmpC ( Table 2). Chaperone genes involved in protein fate, such as dnaJ coding a chaperone protein were also identified. DnaJ acts as a sensor for non-native proteins (Siegenthaler and Christen, 2006) and is involved in many cytoplasmic events, such as promotion of protein folding and translocation of nascent polypeptides (Hartl, 1996).

Insertions Within Regulatory Genes
Further, sequencing also identified insertions in well-known regulatory genes, such as lrp (Calvo and Matthews, 1994). Insertion within fimB, which mediates switching in both directions was also identified. An insertion was identified within the c0918 (yliE) gene ( Table 2). yliE encodes a hypothetical conserved inner membrane protein which contains a phosphodiesterase EAL domain. This protein is involved in hydrolysis of the bacterial second messenger cyclic di-GMP (c-di-GMP), a key factor in processes, such as flagellar motility, biofilm formation, the cell cycle, and virulence of pathogenic bacteria (Jenal and Malone, 2006;Römling et al., 2013). Similarly, another clone contained an insertion in c5041 (kguS) which was identified as a sensor protein of a two-component signaling system involved in α-ketoglutarate utilization. This system is involved in utilization of α-ketoglutarate, an abundant metabolite during UPEC infection, which regulates target genes that encode α-ketoglutarate dehydrogenase and a succinyl-CoA synthetase. This two-component signaling system has been shown to be important for UPEC fitness during UTI (Cai et al., 2013). Also, rstA, a member of the two-component regulatory system RstB/RstA (Calvo and Matthews, 1994) was disrupted in one of the mutants.

Insertions Within Hypothetical Genes
Several of the mutations disrupted genes predicted to encode hypothetical proteins of unknown function, and the roles of such genes for E. coli physiology as well as their influence on expression of type 1 fimbriae and UPEC pathogenesis are unknown ( Table 2). Sequence analysis identified an independent insertion within the yqhG gene and an insertion immediately upstream of the yqhG coding region (Figure 4A), strongly suggesting the involvement of YqhG in type 1 fimbriae production. Thus, we focused the rest of our investigation on this gene of unknown function and its role for production of type 1 fimbriae and UPEC pathogenesis.

Disruption of yqhG Reduces Expression of Type 1 Fimbriae
A transposon was inserted in the opposite orientation of yqhG in one mutant and in the middle of the gene in another mutant (Figure 4A), suggesting that the yqhG gene was involved in regulation of expression of type 1 fimbriae. Since type 1 fimbriae contribute to UPEC pathogenicity (Nielubowicz and Mobley, 2010), the production of type 1 fimbriae was then evaluated by yeast agglutination at the mid-log phase of growth in LB (O.D 0.6) and urine (O.D 0.4). As expected, mutations within yqhG had an effect on the production of type 1 fimbriae ( Figure 4B). After mid-log growth with shaking in LB, and overnight growth in human urine with shaking, the agglutination titer of CFT073 yqhG was reduced by 4-fold as compared to strain CFT073. Agglutination titers of the complemented mutant regained titers similar to that of the WT strain ( Figure 4B).
To confirm that yeast agglutination was mediated by type 1 fimbriae, the assay was also performed in the presence of 2.5% mannopyranose, which blocks type 1 fimbriae-mediated agglutination. As expected, no yeast agglutination was observed when 2.5% mannopyranose was added to the bacteria. These results indicate that the mutation of yqhG caused a substantial decrease of type 1 fimbriae expression. Since the yqhG mutant demonstrated a defect in type 1 fimbriae production ( Figure 4B), we further investigated the ability of the mutant to grow in human urine. Loss of the yqhG in strain CFT073 did not affect growth in human urine.
To confirm that production of type 1 fimbriae was reduced in the yqhG mutant, Western blotting against the type 1 major subunit FimA was determined. Western blotting confirmed an important decrease of the FimA protein in the yqhG mutant compared to parent strain CFT073 and the complemented strain ( Figure 4D).

The yqhG Mutant Demonstrates Reduced Adherence to Human Bladder Epithelial Cells
The adherence of the yqhG mutant to 5,637 human bladder epithelial cells was compared to that of the parental strain CFT073. Figure 5A shows that the adherence of the yqhG mutant was reduced ∼2-fold compared to that of WT strain. Further, the decrease in epithelial cell adherence was rescued by complementation of the yqhG gene. Addition of 2.5% α-dmannopyranose to block the effect of type 1 fimbriae greatly FIGURE 5 | (A) Effect of inactivation of yqhG and production of type 1 fimbriae on adherence of uropathogenic E. coli CFT073 to human bladder epithelial cells in vitro. Adherence of strain CFT073 and its derivatives to human 5,637 bladder epithelial cells in the presence or absence of 2.5% α-d-mannopyranose was determined. (B) Effect of inactivation of yqhG on orientation of the fim promoter switch (fimS) in vitro. The fimS region was PCR amplified, and the product was digested with HinfI. Fragments of different sizes indicate the ON or OFF orientation. All results shown are the mean values and standard deviations for four biological experiments. Statistical significance was calculated by the Student t-test (A). *P < 0.05; **P < 0.005; ***P < 0.0001.
reduced cell association of all strains tested in the cell association assay (Figure 5A).
To determine if the reduction of type 1 fimbriae was due to orientation bias of the phase-variable promoter, we determined orientation of the fimS promoter region. The orientation of fimS was evaluated in strains grown under agitation to mid-log phase in LB broth. Using the procedure described by Stentebjerg-Olesen et al. (2000), we observed that the fim promoter clearly had an increased bias for the OFF position in the yqhG mutant ( Figure 5B).

The yqhG Mutant Demonstrates Reduced Bladder and Kidney Colonization in Mice
Type 1 fimbriae are important for ExPEC colonization of the bladder during UTIs. Since the yqhG mutant demonstrated decreased type 1 fimbriae production, we tested its capacity to cause urinary tract infection in the CBA/J mouse model. Forty-eight hours after urethral inoculation, the yqhG mutant was attenuated 100-fold in bladder and 10,000-fold in kidneys (P < 0.0001) compared to the WT parent strain (Figure 6). Further, the complemented mutant regained the capacity of colonization. This reduction in colonization from inactivation of yghG could be due to reduced production of type 1 fimbriae, and potentially other changes in the ∆yqhG mutant that could decrease colonization of the murine urinary tract (Figure 6).

The yqhG Mutant Demonstrates Increased Motility
Since flagella and swimming motility play a pivotal role in colonization and persistence in the urinary tract (Lane et al., 2005), we investigated whether the loss of yqhG affected swimming motility. The yqhG mutant was considerably more motile than the UPEC CFT073 parental strain. Complementation of the yqhG mutant (strain QT5235) effectively restored motility to wild-type levels. Further, a fimS Locked-ON reference strain was shown to be non-motile in the swimming assays (Figure 7).

yqhG Contributes to Oxidative Stress Resistance
During the course of a UTI, UPEC come across a variety of environmental stresses that can potentially limit survival and growth . Envelope stress response pathways are likely to be critical for UPEC, in order to detect and respond to potentially harmful environmental insults during the course of infection. In an effort to determine the mechanism by which yqhG influences type 1 fimbriae expression, we first sought to determine if this gene is involved in hydrogen peroxide resistance. Our observations revealed that the yqhG strain was more sensitive to oxidative killing compared to wild-type strain CFT073. This result indicates that yqhG contributes to resistance to oxidative stress mediated by hydrogen peroxide (Table 3). Further, screening of specific transposon mutants that were also shown to have decreased expression of type 1 fimbriae including clones with insertions in the slp or yegQ genes also showed increased sensitivity to hydrogen peroxide-mediated oxidative stress ( Table 3).

The pst Mutant of UPEC CFT073 Is Also Sensitive to Osmotic and Oxidative Stress
In a previous study, in avian pathogenic E. coli (APEC) strain χ7122, inactivation of the pst system mimicked phosphatelimiting conditions and caused pleiotropic effects . In the APEC pst mutant, membrane homeostasis was altered and included modification of phospholipids . Accordingly, the same mutant appeared to modulate the expression of some genes regulating antioxidant activities FIGURE 6 | Deletion of yqhG reduces colonization of the murine urinary tract. CBA/J mice were infected transurethrally and the animals were euthanized, and organs were collected 48 h post-infection. Each data point represents a sample from an individual mouse, and horizontal bars indicate the medians. Two independent infections were performed: with CFT073 WT and CFT073 yqhG and with CFT073 WT and CFT073 yqhG-Tn7T-Cm::yqhGH. Each kidney was sampled separately (Mann-Whitney test). *P < 0.05; **P < 0.005; ***P < 0.0001.
FIGURE 7 | Effect of deletion of yqhG on motility. Diameters of swimming motility of CFT073, mutants and complemented mutant. Data represent the averages of five separate experiments. Error bars represent the SEM. Significant differences in motility between mutants and complemented mutants were determined using a paired Student t-test. *P < 0.05; **P < 0.005; ***P < 0.0001. . In the UPEC strain CFT073 pst mutant, attenuation of urinary tract virulence was shown to mainly be attributed to reduced production of type 1 fimbriae (Crépin et al., 2012b). As stress resistance is crucial for the survival of UPEC strains in the host, we also evaluated the capacity of the UPEC CFT073-derived pst mutant to resist oxidative and osmotic stresses. Sensitivity to hydrogen peroxide was analyzed from exponential growth cultures of CFT073 and the pst derivative by using the H 2 O 2 agar overlay diffusion method. Under phosphate-sufficient conditions (LB), the pst mutant was more sensitive to oxidative stress, as diameters of inhibition zones were significantly larger with the pst mutant than with the CFT073 parental strain (P < 0.001) ( Table 2). Complementation of the pstCAB mutant restored the wild-type phenotype ( Table 3). Given the significant decrease in the colonization of the mouse bladder by the pst mutant, we also tested whether this strain displayed increased susceptibility to osmotic stress by using an established protocol (Pavanelo et al., 2018). We, therefore, tested the growth of the wild-type, pst mutant, and a complemented mutant on modified LB agar containing different concentrations of NaCl or urea. Both strains were able to grow on LB agar with 0.3 M NaCl ( Figure 8A) and with 0.3 M urea (Figure 8B), and no strain could grow on LB agar with 1 M NaCl and 1 M urea. In contrast, the pst mutant was significantly more sensitive to 0.6 M NaCl ( Figure 8C) and 0.6 M urea ( Figure 8D) compared to parent strain CFT073. The complemented mutant grew similarly to the WT strain (Figure 8). Taken together, the pst mutant was found to show increased sensitivity to both oxidative and osmotic stresses.

DISCUSSION
The ability to adhere to host epithelial cells is an important factor for initial colonization and persistence during a UTI. Therefore, type 1 fimbriae are crucial for the establishment of UPEC infections (Schaeffer et al., 1987). Bacterial adherence to the uroepithelium limits the effect of shear forces produced by urine flow and thereby improves colonization (Thomas et al., 2002). Urine is considered to be a nutrient limiting environment with relatively low levels of available sugars and metabolites. Consequently, UPEC metabolism is tightly regulated and highly responsive to nutrient availability, and UPEC adapted to utilization of a wide range of nutrients from this nutrientlimited environment (Mann et al., 2017). Further, most bacteria are unable to thrive within the urinary tract environment due to its high osmolarity, elevated urea concentrations, low pH, and limited iron availability (Mann et al., 2017). Due to this transition, bacteria that enter the urinary tract encounter a harsh environment and are subject to numerous stresses, and stringent competition due to a drastic reduction in the abundance of nutrients. Further, environmental cues, such as pH and osmolality, have been shown to regulate fim genes and to affect the orientation of the fim switch (Schwan et al., 2002).
The expression of type 1 fimbriae is controlled by an invertible DNA element that upon inversion changes the promoter orientation through two site-specific recombinases, FimB and FimE. Thus, affecting the transcription of the fimAICDFGH genes (Abraham et al., 1985). This inversion phenomenon known as phase variation, reversibly switches between the expression of type 1 fimbriae (Phase-ON) and loss of expression (Phase-OFF) (Klemm, 1986). Besides the fim gene cluster, other genes and their gene products contribute to the expression of type 1 fimbriae. The global regulator histone-like nucleoid-structuring protein (H-NS), integration host factor (IHF), leucine-responsive protein (Lrp), and cyclic adenosine monophosphate (cAMP) receptor protein (CRP)/cAMP, control the expression of type 1 fimbriae directly and indirectly (Blomfield et al., 1997;Olsen et al., 1998;Kelly et al., 2006). Other proteins affect type 1 fimbriae expression in E. coli such us OmpX (Otto and Hermansson, 2004), IbeA, and IbeT (Cortes et al., 2008), although mechanisms of control are unknown. The regulatory alarmone, ppGpp, has been connected to the regulation of the fim operon (Åberg et al., 2006). Alteration of phosphate metabolism through inactivation of the phosphate specific transporter (pst) was shown to contribute to expression of type 1 fimbriae and attenuated UPEC virulence (Crépin et al., 2012b). Further, inactivation of pst was linked to increased production of the signaling molecule c-di-GMP, which in turn decreased the expression of type 1 fimbriae in UPEC CFT073 (Crépin et al., 2017). It has been shown that under a slightly acidic pH and low salt growth conditions found on the vaginal surface, that proteins, such as SlyA or RcsB may activate fimB and prevent H-NS from binding, allowing type 1 fimbriae to be expressed on the surface of the UPEC cells (Schwan et al., 2007;McVicker et al., 2011). Recently, the treA gene coding for a periplasmic trehalase that contributes to osmotic stress resistance was also shown to affect type 1 fimbriae production in an APEC strain and this mutation significantly reduced adherence to and invasion of epithelial cell and bladder colonization in a murine model of UTI (Pavanelo et al., 2018). Taken together, there is a body of evidence indicating that type 1 fimbriae expression is important for UPEC colonization of the urinary tract and that multiple factors, including adaptation to osmotic and oxidative stress, that influence expression of these fimbriae play a role in E. coli UTI pathogenesis.
Given the importance of type 1 fimbriae and control of its expression, in the present report we devised a means to randomly identify different genes involved in the regulation of type 1 fimbriae by using a luxCDABE reporter fusion and genetic screening of a transposon bank. We constructed a fimS-lux reporter fusion integrated on a single-copy at the chromosomal attTn7 of pyelonephritis strain CFT073 (Figure 1B). Integrating the lux reporter into the chromosome results in a reduced level of promoter expression compared to fusions on multicopy vectors, but has the added advantage of stability of the signal during long term localization studies and more relevant comparison to regulatory effects on the native fim switch, as the single-copy fusion will not be as biased by titration of regulator proteins or recombinases that could occur with multi-copy fusion vectors. Moreover, the luciferase bioluminescence system may overcome some limitations of fluorescent reporters used in in vivo imaging, such us background signals associated with cellular autofluorescence, poor penetration of the excitation wavelength, slow turnover of the fluorescent protein, etc. (Riedel et al., 2007).
The fimS-lux fusion was shown to undergo a change in signal in accordance to changes in regulation of the fimA promoter observed under different pH conditions (Figure 2A), demonstrating its potential value at investigating environmental cues that could affect transcription of the fim gene cluster. A shift from pH 4.4 to a neutral pH 7.0 resulted in a significant increase of fimS expression. Furthermore, and in accordance with previous reports (Schwan et al., 2002;Schwan and Ding, 2017), the expression of fimS was increased when bacteria were cultured in LB (neutral pH) and decreased at lower pH (Figure 2A). The in vitro results with the fim-lux fusions were similar to previous studies (Schwan et al., 2002;Schwan and Ding, 2017), and helped validate the reporter system that we developed. Our lux fusion was highly sensitive with low background noise, which makes this reporter rapid enough to enable a delicate monitoring of quick response kinetics. Indeed, such vectors have also been developed for transposon-based systems with random integration of lux into the chromosomes of both Gram-negative and Gram-positive bacteria Francis et al., 2001). However, these systems are based on random integration of the transposon into the chromosome, followed by selection of clones which retain viability but demonstrate high levels of lux expression. Hence, our lux system seems more efficient with single-copy integration of recombinant genes at the attTn7 site that does not require selective pressure and can be used in a variety of Enterobacteriaceae. This system provides a useful tool for studying promoter regulation without introducing unforeseen genetic changes that influence the behavior of the strain in vitro and in vivo. The application of the lux reporter in combination with transposon mutagenesis and high-throughput sequencing herein provided a novel and valid approach to identify specific genes and begin to dissect genetic pathways linked to expression of type 1 fimbriae.
Our transposon library screening resulted in the identification of numerous insertions that deregulated expression of type 1 fimbriae. In this study, we initially screened 5,904 transposon mutants by measuring the level of luminescence following growth on LB broth and phenotypic screening for production of type 1 fimbriae by using a yeast agglutination assay (Figure 3). Here, we searched for genes with a significant increase or decrease in luminescence levels from specific transposon mutants compared to the control strain (CFT073, fimS-lux). The insertion of transposons in specific clones were shown to repress or activate lux expression fimS and hence deregulate type 1 fimbriae production ( Figure 3A). Using high throughput sequencing, we then mapped the Tn10 insertion sites of these mutants (Supplemental Figure 3), leading to the identification of numerous genes that significantly altered type 1 fimbriae production ( Table 2). In addition to known structural and regulatory genes, genes identified included those involved in biogenesis of type 1 fimbriae and other fimbriae, amino acid biosynthesis, membrane transport, chaperones involved in protein fate and genes that are currently of unknown function. Confirmation of the role of these genes in type 1 fimbriae expression via the construction and characterization of specific mutants will be required to more clearly determine their mechanisms of action in the regulation of expression type 1 fimbriae and potential roles in UTI pathogenesis. Several clones had insertions within genes with metabolic functions. One of these clones disrupted tdcB (catabolic threonine dehydratase). Many nutrient transport systems and genes related to carbohydrate metabolism have been reported to be involved in the virulence of ExPEC strains. For example, the periplasmic trehalase treA affects type 1 fimbriae production and virulence of APEC strain MT78 (Pavanelo et al., 2018). The metabolic frz operon has also been shown to link the metabolic capacity of ExPEC with expression of genes required for adherence to the bladder epithelium; the presence of the frz operon favors the ON orientation of the invertible type 1 fimbriae promoter (Rouquet et al., 2009). The two-component-system (TCS), KguSR, involved in the control of utilization of α-ketoglutarate, has also been shown to be important for UPEC fitness during UTI (Cai et al., 2013). Interestingly, this system was disrupted in our mutant bank. As such, the roles of this TCS in type 1 fimbriae expression in UPEC merit further investigation. Moreover, proteins involved in the transport and catabolism of sialic acid, xylose, arabinose, and the biosynthesis of arginine and serine are highly expressed in UPEC cultured in human urine (Alteri et al., 2009). It has also been reported that sialic acid regulates type 1 fimbriae by inhibiting FimB switching in UPEC (Sohanpal et al., 2004). Tn insertions also disrupted well-studied regulators, Lrp, a global regulator of genes involved in metabolic functions within E. coli including fimbriae production (Brinkman et al., 2003;Kelly et al., 2006) and fimB which regulates phase variable expression of the fim operon (Blomfield et al., 1997). It is intriguing to see a potential connection of iron acquisition mechanisms and type 1 fimbriae production as disruption of the aerobactin precursor iucC (de Lorenzo and Neilands, 1986) affected the fimbriae production. However, the specific mechanisms underlying how certain genes involved in metabolism influence the expression of type 1 fimbriae and to what extent this alone may influence UPEC pathogenesis during UTI remain to be elucidated.
Further, some of the genes in our bank have been reported earlier for the correlation with strong virulence and pathogenesis of UPEC. Loss of nadB rendered NAD auxotrophy and contributes to the virulence in Shigella (Prunier et al., 2007) however the colonization of CFT073 and UTI89 in murine UTI model was not influenced by NAD auxotrophy (Li et al., 2012). We identified an insertion in rstA which is a part of low pH sensing RstBA two-component system which is directly involved in the regulation of csgD, which encodes a regulator responsible for curli and cellulose production (Ogasawara et al., 2010). Interestingly, mutation in emrK in E. coli caused a significant drop in biofilm formation (Matsumura et al., 2011). So, these observations show the possible correlation between inhibition of fimbriae and a reduction in biofilm formation.
This relation has been documented before in terms of aerobic respiration in the bladder. Biofilm production in UPEC has been shown to be affected by different terminal electron acceptors (Eberly et al., 2017). In addition, type 1 fimbriae production was reduced in the absence of oxygen, and UPEC strain UTI89 had increased type 1 fimbriae production on the air exposed region of biofilm due to increased oxygen level (Floyd et al., 2015). In conjugation with biofilm forming capacity, cytochrome bd provided a fitness advantage for UTI89 under hypoxic growth conditions as well as increased nitric oxide tolerance (Beebout et al., 2019). So, our insertion in cytochrome c-type gene, yecK/torY, may also affect the adherence due to type 1 fimbriae as well as respiration in various oxygen gradient in the complex biofilm community.
Interestingly, many of the genes identified in our bank that influenced type 1 fimbriae expression are uncharacterized genes of hypothetical or unknown function. We were particularly interested in further investigating the role of the yqhG gene encoding a hypothetical protein containing DUF3828 domains that is conserved in E. coli. Although, the function of this gene is still unknown, transcription of yqhG was shown to be positively regulated by the BglJ-RcsB complex (Salscheider et al., 2013) in E. coli K-12. A putative binding site for BglJ-RcsB with a significant score was also identified upstream of the yqhG promoter (Salscheider et al., 2013). Interestingly, RcsB is the response regulator of the Rcs phosphorelay which is conserved in Enterobacteriaceae. RcsB plays a pleiotropic role in the control of biofilm formation, motility behavior and responds to membrane stress, specifically outer membrane stress, and is best known for its positive regulatory effect on capsule synthesis . Further, BglJ is a positive DNA-binding transcriptional regulator of transport and utilization of the aromatic β-glucosides arbutin and salicin (Madhusudan et al., 2005). It has been shown that it is completely dependent on RcsB (Venkatesh et al., 2010). It will therefore be of interest to further investigate potential regulatory links between these regulators and yqhG expression in UPEC.
A more pronounced reduction of yeast agglutination occurred when the yqhG mutant was grown to mid-log phase in LB or urine compared to O/N growth. Similarly, other mutations in genes, such as the pst system also resulted in a marked change in expression of type 1 fimbriae during growth to mid-log phase compared to after overnight static growth (Crépin et al., 2012b). Because the CFT073 yqhG mutant displayed a decreased capacity to agglutinate yeast in LB and urine (Figures 4B,C), we performed Western blotting against type 1 fimbriae to investigate any effect on type 1 fimbriae production. As shown in Figure 4D in comparison to the WT strain, the mutant had a reduced production of type 1 fimbriae. This reduction in type 1 fimbriae was also in line an increased bias for the OFF position with fim promoter in the yqhG mutant in LB broth at O.D. 600nm 0.6 ( Figure 5B). Interestingly, we also found that the yqhG mutant was more motile than the WT strain (Figure 7). UPEC strains coordinately regulate motility and adherence to mediate colonization and dissemination during the pathogenesis of UTIs. It is widely believed that when adhesin genes are expressed, motility genes are repressed and vice versa (Simms and Mobley, 2008). They represent opposing forces. Thus, by mediating adherence, fimbriae would promote a sessile state and flagellarbased motility would be expected to be decreased. By contrast, increased motility by flagella would reduce the ability of bacteria to adhere at one site. Accordingly Bryan et al. (2006) and Lane et al. (2007) have shown that constitutive expression of type 1 fimbriae (CFT073 fim L-ON) leads to repression of swimming motility in strain CFT073. The yqhG mutant also exhibited increased binding to Congo Red on agar plates, suggesting a possible increase in cellulose and curli production compared to the wild-type strain. The decreased expression of type 1 fimbriae may therefore also lead to increased production of other adhesins or biofilm associated factors under certain growth conditions. Further experiments, will be required to confirm whether loss of yqhG may promote expression of other fimbriae while reducing expression of type 1 fimbriae.
In the murine UTI model, the yqhG mutant was attenuated ( Figure 5). Loss of yghG in CFT073 caused an important decrease in colonization of both the bladder and kidneys, whereas loss of type 1 fimbriae by deletion of the fim operon, mainly results in a decrease in colonization of the bladder in the mouse model (Gunther et al., 2002). As such, we investigated whether loss of yqhG also affected resistance to certain stress conditions including oxidative stress and osmolarity. A yqhG derivative of CFT073 was significantly more sensitive than its wild-type parent to oxidative stress from H 2 O 2 challenge (Table 3), and complementation of this mutant with a single-copy of yqhG restored wild-type resistance to H 2 O 2 killing. Although, it is clear that YqhG plays a role in regulating expression of type 1 fimbriae and promoting adherence of UPEC CFT073 to host cells, YqhG likely plays a greater role in UPEC, including adaptation to environmental stresses, as it is also required for resistance to oxidative stress. A plausible mechanism for this drop could be due to reduced catalase activity in the mutant to counteract the oxidative burst from immune cells during infection. The yghG mutant colonies also demonstrated less bubbling upon addition of H 2 O 2 compared to wild type. So, mutation in yqhG may also lead to increased sensitivity to reactive oxygen radicals as well as reduced production of type 1 fimbriae. Nevertheless, our results shed new light on the importance of yqhG for UPEC virulence, and it will be of interest to further elucidate what other factors that are, directly or indirectly, regulated by yqhG, including type 1 fimbriae, and determine its importance for resistance of E. coli to host innate immune response during infection.
Finally, we investigated whether inactivation of pst, in addition to repression of expression of type 1 fimbriae also reduced resistance to stresses including oxidative and osmotic stress. Decreased virulence of the pst mutant of UPEC CFT073 was mainly attributed to the decreased expression of type 1 fimbriae (Crépin et al., 2012b). The ability sense, adapt to, and resist different types of stress can also play an important role in regulation of gene expression, including regulation of type 1 fimbriae. As such, we investigated the capacity of the pst mutant to resist oxidative and osmotic stresses. The conditions were chosen to simulate the effects of NaCl and urea that UPEC cells may encounter during colonization of the urinary tract. Urea can permeate through the cell membrane and destabilize the native structure of proteins inside cells (Withman et al., 2013). According to our results, CFT073 showed a higher resistance to 0.6 M urea and 0.6 NaCl than the pst mutant grown to midlog phase (Figures 7C,D). Similarly, the pst mutant was much more sensitive to hydrogen peroxide than the CFT073 parent strain (Table 3). Therefore, inactivation of pst in CFT073 resulted in increased sensitivity to both osmotic and oxidative stress, and this may importantly also in part be linked to changes in levels of expression of type 1 fimbriae. In APEC strain χ7122, inactivation of the Pst system induced deregulation of phosphate sensing and important changes in cell surface composition that led to reduced virulence in a chicken infection model, decreased production of type 1 fimbriae and lower resistance to oxidative stress (Lamarche et al., 2005;Crépin et al., 2008). Several studies concerning osmotic stress and expression of type 1 fimbriae were also reported for UPEC. In UPEC strain NU149, type 1 fimbriae expression was downregulated under osmotic stress caused by NaCl (Schwan et al., 2002). Further, loss of treA in ExPEC strain MT78 also resulted in a change in osmotic resistance to urea, concomitant with a decreased expression of type 1 fimbriae and reduced urinary tract colonization in the mouse model (Pavanelo et al., 2018). Transcriptome analyses also showed upregulation of type 1 fimbriae expression and the genes that are regulated by the osmotic stress response in CFT073 during UTI (Snyder et al., 2004).
Overall, generation of a transposon bank and a single-copy lux-based reporter fusion system integrated at the attTn7 site in UPEC strain CFT073, has led to successful identification of insertions in a number of genes including heretofore unknown sites that altered expression of type 1 fimbriae. Interestingly, these insertions include a variety of genes involved in a diversity of functions including protein fate and synthesis, energy metabolism, adherence, transcriptional regulation, and transport, and genes of hypothetical or unknown function including yqhG, that we have shown to play an important role in UPEC colonization in the mouse model as well as resistance to oxidative stress. It will be of interest to more fully elucidate how some of these different systems influence expression of type 1 fimbriae as well as their potential roles in metabolism and bacterial regulatory networks as well as sensing, adaptation and resistance to environmental stresses, such as osmotic and oxidative stresses that may be encountered during the course of colonization and infection of the host.

DATA AVAILABILITY
The datasets generated for this study are available on request to the corresponding author.

AUTHOR CONTRIBUTIONS
HB was the primary author and performed most of the experiments and writing of the manuscript. PP and HH contributed to some of the experiments and writing of the text. SH contributed the technical assistance including mouse infections with other co-authors. CD conceived the planning of the study, design of experiments, mentored the researchers, and revised the manuscript. Supplemental Table 1 | Primers used in this study.
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