Original Research ARTICLE
Exosome-transmitted miR-25 induced by H. pylori promotes vascular endothelial cell injury by targeting KLF2
- 1960th Hospital of the PLA, China
Background: Increasing evidence has shown that Helicobacter pylori (H. pylori) is involved in coronary heart disease (CHD), but the underlying mechanism remains unclear.
Methods: The expression of miR-25 and mRNAs was measured using qRT-PCR. Protein levels were detected using western blotting. Exosome was estimated using electron microscope. The target gene of miR-25 was identified using luciferase report system.
Results: H. pylori infection increases the expression of miR-25 in gastric epithelial cells and is associated with increased levels of exosome-transmitted miR-25 in human peripheral blood. Mechanistic investigation shows Kruppel-like factor 2 (KLF2) as a direct target of exosome-transmitted miR-25 in vascular endothelial cells, and miR-25/KLF2 axis regulates the NF-κB signaling pathway, resulting in increased expression of IL6, MCP-1, VACM-1 and ICAM-1.
Conclusion: Our findings suggest that miR-25/KLF2 axis may be a potential therapeutic target for H. pylori-associated CHD and high level of exosome-transmitted miR-25 in peripheral blood may be a potential risk of CHD.
Keywords: MiR-25, Helicobacter pylori, coronary heart disease, NF-κB – Nuclear factor kappa B, KLF2 protein, human
Received: 27 Jun 2019;
Accepted: 08 Oct 2019.
Copyright: © 2019 Wang, Li, Liu, Dong, Zhang, Huang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mx. Tong-jian Wang, 960th Hospital of the PLA, Jinan, Shandong Province, China, email@example.com