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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2019.00390

Identification of Salmonella Bredeney Resistant to Third-Generation Cephalosporins in Saudi Arabia

 Ayman A. Alkraiem1, 2, 3*,  Yingchun Zeng4, Xixiang Huo4, Guang Yang2* and  Tie Chen1*
  • 1Department of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, China
  • 2Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, China
  • 3Department of Biology, College of Science, Taibah University, Saudi Arabia
  • 4Hubei Provincial Center for Diseases Control and Prevention, China

The rapidly increasing prevalence and spread of antibiotic-resistant Salmonella throughout the world have become a thorny problem that poses a serious threat to human health. It is speculated that the abuse of antibiotic usages and high density of human traveling and Mass Gatherings (MGs) accelerate this threat. To explore the hypothesis, 13 Salmonella isolates from Mecca area of Saudi Arabia and 15s from China as the control group were performed with the typical methods of serotype identification, antibiotic resistance tests, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Our results presented that the isolates from China showed greater serotype diversity and a higher antimicrobial resistance rate, which were consistent with results in other studies from China. In contrast, the Saudi Arabian isolates were mainly identified as Serovar Bredeney and resistant to very limited antibiotics. To a biggest surprise, one of the Bredeney isolates was resistant to third-generation cephalosporins but sensitive to all other tested antibiotics. To confirm the results and understand the underlying molecular mechanisms of these isolates, a whole genome sequencing (WGS) was performed. We found that several cephalosporins resistance-associated fragments were shared with other strains, but one sequence (LEN-23) was very unique to this strain. Therefore, we concluded that this study, for the first time, reported the emergence of Salmonella Bredeney resistant to third-generation cephalosporins in Saudi Arabia.

Keywords: Salmonella enterica, Multilocus Sequence Typing, Drug Resistance, pulsed-field gel electrophoresis, Bredeney serotype, Cephalosporins

Received: 02 Apr 2019; Accepted: 30 Oct 2019.

Copyright: © 2019 Alkraiem, Zeng, Huo, Yang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Ayman A. Alkraiem, Department of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, China, ayman_ah_k@hotmail.com
Mx. Guang Yang, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China, gyang-hust@hust.edu.cn
Prof. Tie Chen, Department of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, China, chentie@hust.edu.cn