%A Kushugulova,Almagul %A Löber,Ulrike %A Akpanova,Saniya %A Rysbekov,Kairat %A Kozhakhmetov,Samat %A Khassenbekova,Zhanagul %A Essex,Morgan %A Nurgozhina,Ayaulym %A Nurgaziyev,Madiyar %A Babenko,Dmitriy %A Markó,Lajos %A Forslund,Sofia K. %D 2021 %J Frontiers in Cellular and Infection Microbiology %C %F %G English %K Mare's milk,microbiome,16S rRNA gene sequencing,collateral antibiotic effect,intestinal immunity Abstract %Q %R 10.3389/fcimb.2021.622735 %W %L %M %P %7 %8 2021-April-21 %9 Original Research %+ Almagul Kushugulova,Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University,Kazakhstan,almagul.kushugulova@gmail.com %+ Almagul Kushugulova,Kazakhstan Society of Human Microbiome Researchers,Kazakhstan,almagul.kushugulova@gmail.com %+ Almagul Kushugulova,SaumalBioTech,Kazakhstan,almagul.kushugulova@gmail.com %# %! Mare’s milk reduces side antibiotic effect %* %< %T Dynamic Changes in Microbiome Composition Following Mare’s Milk Intake for Prevention of Collateral Antibiotic Effect %U https://www.frontiersin.org/articles/10.3389/fcimb.2021.622735 %V 11 %0 JOURNAL ARTICLE %@ 2235-2988 %X IntroductionProbiotics and prebiotics are widely used for recovery of the human gut microbiome after antibiotic treatment. High antibiotic usage is especially common in children with developing microbiome. We hypothesized that dry Mare’s milk, which is rich in biologically active substances without containing live bacteria, could be used as a prebiotic in promoting microbial diversity following antibiotic treatment in children. The present pilot study aims to determine the impacts of dry Mare’s milk on the diversity of gut bacterial communities when administered during antibiotic treatment and throughout the subsequent recovery phase.MethodsSix children aged 4 to 5 years and diagnosed with bilateral bronchopneumonia were prescribed cephalosporin antibiotics. During the 60 days of the study, three children consumed dry Mare’s milk whereas the other three did not. Fecal samples were collected daily during antibiotic therapy and every 5 days after antibiotic therapy. Total DNA was isolated and taxonomic composition of gut microbiota was analyzed by 16S rRNA amplicon sequencing. To assess the immune status of the gut, stool samples were analyzed by bead-based multiplex assays.ResultsMare’s milk treatment seems to prevent the bloom of Mollicutes, while preventing the loss of Coriobacteriales. Immunological analysis of the stool reveals an effect of Mare’s milk on local immune parameters under the present conditions.