Roles of TLR7 in Schistosoma japonicum Infection-Induced Hepatic Pathological Changes in C57BL/6 Mice

S. japonicum infection can induce granulomatous inflammation in the liver of the host. Granulomatous inflammation limits the spread of infection and plays a role in host protection. Toll-like receptor 7 (TLR7) is an endosomal TLR that recognizes single-stranded RNA (ssRNA). In this study, the role of TLR7 in S. japonicum infection-induced hepatitis was investigated in both normal and TLR7 knockout (KO) C57BL/6 mice. The results indicated that TLR7 KO could aggravate S. japonicum infection-induced damage in the body, with less granuloma formation in the tissue, lower WBCs in blood, and decreased ALT and AST in the serum. Then, the expression of TLR7 was detected in isolated hepatic lymphocytes. The results indicated that the percentage of TLR7+ cells was increased in the infected mice. Hepatic macrophages, DCs, and B cells could express TLR7, and most of the TLR7-expressing cells in the liver of infected mice were macrophages. The percentage of TLR7-expressing macrophages was also increased after infection. Moreover, macrophages, T cells, and B cells showed significant changes in the counts, activation-associated molecule expression, and cytokine secretion between S. japonicum-infected WT and TLR7 KO mice. Altogether, this study indicated that TLR7 could delay the progression of S. japonicum infection-induced hepatitis mainly through macrophages. DCs, B cells, and T cells were involved in the TLR7-mediated immune response.


INTRODUCTION
Schistosomiasis is the most important helminth disease in the world from a public health perspective (Llanwarne and Helmby, 2021). It is estimated that approximately 230 million people in the world are still under the threat of schistosomiasis (Lackey and Horrall, 2020). Schistosoma mansoni and Schistosoma japonicum (S. japonicum) account for the majority of global intestinal schistosomiasis cases, and S. japonicum is prevalent in East Asia .
During infection with S. japonicum, cercariae, juvenile worms, adult worms and eggs can cause damage to the host (Llanwarne and Helmby, 2021). Eggs are deposited in the liver and intestinal wall of the host and can secrete soluble egg antigen (SEA) (De Marco et al., 2019) and induce egg granuloma formation and liver fibrosis (Schwartz and Fallon, 2018). Granulomatous inflammation can lead to pathological changes, but granulomatous inflammation limits the spread of infection and plays a role in host protection (Hams et al., 2013). Extensive granulomatous fibrosis causes portal hypertension, which results in irreversible cirrhosis and advanced schistosomiasis, leading to host death (Lackey and Horrall, 2020).
The liver microenvironment is a site of immune regulation and tolerance induction, with a unique constituency of innate and adaptive immune cells (Williamson et al., 2019). Liver resident antigen presenting cells (APCs) that have been shown to regulate inflammatory and T cell-mediated immune responses include dendritic cells (DCs), macrophages, sinusoid-lining endothelial cells, and hepatic stellate cells (Racanelli and Rehermann, 2006). Liver-resident macrophages, also called Kupffer cells, account for approximately 90% of the total tissue macrophages in the body. Recent studies have shown that the liver is a primary surveillance organ for intravascular infections and is especially important for filtering pathogens via KCs to maintain blood sterility Wohlleber and Knolle, 2016).
S. japonicum infection can induce a class Th2 immune response in both humans and animals (Farwa et al., 2018). It was reported that many kinds of immune cells, such as DCs, Th cells, B cells, and gd T cells, take part in the course of the inflammatory response Tang et al., 2019;Xiao et al., 2020). Many kinds of cytokines, including IL-4, IL-10, IL-13, IL-17, and TGF-beta, play important roles in mediating these immune responses Osada et al., 2019;Huwait et al., 2021). In addition, pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs) and mannose receptors (CD206), which recognize pathogen-associated molecular patterns (PAMPs) on S. japonicum, were reported to modulate the immune response (Paveley et al., 2011;Wang et al., 2017).
TLR7 is an endosomal TLR that recognizes single-stranded RNA (ssRNA) and responds to imidazoquinoline compounds such as imiquimod or resiquimod (Ernst et al., 2020). TLR7 was originally identified as a sensor for single stranded RNA (ssRNA) and plays an important role in fighting against pathogens, such as viruses and bacteria (Parra et al., 2018;Heni et al., 2020). In parasite research, TLR7 ccould mediate early innate immune responses to malaria (Baccarella et al., 2013). Moreover, TLR7 was reported triggering in neutrophils regulated early innate functions with major consequences on subsequent disease evolution in Cutaneous Leishmaniasis (Regli et al., 2020). Recently, TLR7/8 agonists were found to be therapeutic agents against bacteria (Saroa et al., 2019) and virus infection (Boni et al., 2018). In addition, TLR7/8 agonists were identified as effective candidate adjuvants in vaccine research (Hu et al., 2020). Moreover, TLR7 was shown to be protective in atherosclerosis (Karadimou et al., 2017). The TLR7 agonist R848 was reported to promote survival in cancer (Michaelis et al., 2019).
Here, the role of TLR7 in S. japonicum infection was investigated in the livers of C57BL/6 mice, and the mechanism was explored.

Mice, Parasites, and Infection
Female C57BL/6 mice were purchased from the Animal Experimental Center of Guangzhou University of Chinese Medicine (Guangzhou, China), and TLR7 -/mice (B6.129S1-Tlr7 tm1Fl v/J, strains: 008380) were purchased from the Jackson Laboratory (Bar Harbor, USA). All mice were maintained under specific pathogen-free conditions and used at 6-8 weeks of age.
S. japonicum cercariae were shed from naturally infected Oncomelania hupensis snails, which were purchased from Jiangsu Institute of Parasitic Disease (Wuxi, China). The mice were infected as previous reported (Cha et al., 2020). The snails containing S. japonicum cercariae were placed in dechlorination water at room temperature and placed in a light environment for about 1h. After the activity and quantity of cercariae met the experimental requirements, a sterile loop was used to transfer the S. japonicum cercariaecontaining water on a piece of clean cover slide, and the number of cercariae was counted under a microscope. The abdominal skin of mice was prepared by shaving, and then the cover slide with 40 ± 5 S. japonicum cercariae was put on the abdominal skin in close contact for 10 min.
After the whole infection process was completed, mice in the infected group and control group were fed in the Experimental Animal Center of Guangzhou Medical University. Animal experiments were performed in strict accordance with the regulations for the Administration of Affairs Concerning Experimental Animals (S2020-055), and all efforts were made to minimize suffering.

Histology Studies
Parts of the livers were cut and perfused three times with 0.01 M phosphate-buffered saline (pH = 7.4), fixed in 10% formalin, embedded in paraffin, and sectioned. The slices were stained by standard haematoxylin-eosin (H&E) staining and examined by light microscopy under 100× magnification.

Biochemical Assays
Serum levels of ALT and AST were tested using biochemical kits (Tellgen Life Technology, Shanghai, China), and detected by Beckman Coulter AU5800 (California. USA).

Isolation of Immune Cells
Mice were sacrificed, and the livers were digested with a LIVER dissociation kit (Miltenyi Biotec, Germany) and dissociated into a cell suspension. Then, immune cells were isolated by Ficoll-Hypaque (DAKEWE, SZ, China) density gradient centrifugation from the cell solution. Isolated cells were washed twice in HBSS and resuspended at 2 × 106 cells/ml in complete RPMI 1640 medium supplemented with 10% heat-inactivated foetal calf serum (FCS), 100 U/ml penicillin, 100 µg/ml streptomycin, 2 mM glutamine, and 50 µM 2-mercaptoethanol.

RNA Preparation for Real-Time PCR
Lymphocytes were generated following previously described procedures. Total RNA was isolated from the liver immune cells of infected and naive mice using TRIzol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) following the manufacturer's instructions. cDNA was synthesized with HiScript ® III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech, China), and mRNA expression was determined with ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech, China) according to the manufacturer's instructions. The TLR7 primers were synthesized from Invitrogen (Shanghai, China) as follows: 5′-CCA CAT TCA CTC TCT TCA TTG G-3′ (forward) and 5′-GGT CAA GAA CTT CCA GCC TG-3′ (reverse). The b-actin primers were synthesized from Invitrogen (Shanghai, China) as follows: 5′-CCG TAA AGA CCT CTA TGC CAC AC-3′ (forward) and 5′-GGG TGT AAA ACG CAG CTC AGT A− 3′ (reverse). Real-time PCR reaction system was prepared according to the kit instructions, and the system was heated to 95°C for 30 seconds, followed by 40 cycles of heating up (95°C for 5 seconds) and cooling down (60°C for 30 seconds). Finally, the melting curve was collected. Real-time PCR amplification was performed using CFX96 Touch fluorescent quantitative PCR (Bio-Rad, Hercules, CA). Amplification of b-actin was used as an internal control.

Cell Surface Staining
Cells were washed twice in PBS and blocked in PBS buffer containing 1% BSA for 30 min. Then, the cells were stained with conjugated antibodies that were specific for cell surface antigens for 30 min at 4°C in the dark. The stained immune cells were analysed by using flow cytometry (Beckman Coulter, Fullerton, CA), and the results were analysed with CytoExpert 2.3 software (Beckman Coulter).

Intracellular Cytokine and Molecular Staining of Cells
Single-cell suspensions from the liver were stimulated with 20 ng/ mL phorbol 12-myristate 13-acetate (PMA) plus 1 µg/mL ionomycin for 5 h at 37°C under a 5% CO 2 atmosphere. Brefeldin A (10 g/mL, Sigma, Shanghai, China) was added during the last 4 h of incubation. Cells were washed twice in PBS, fixed with 4% paraformaldehyde, and permeabilized overnight at 4°C in PBS buffer containing 0.1% saponin (Sigma), 0.1% BSA, and 0.05% NaN 3 . Cells were then stained for 30 min at 4°C in the dark with conjugated antibodies specific for cell surface antigens as well as intracellular cytokines or proteins. The expression phenotypes of the antibody-labelled immune cells were analysed by flow cytometry (Beckman Coulter, Fullerton, CA), and the results were analysed with CytoExpert 2.3 software (Beckman Coulter). Isotypematched cytokine controls were included in each staining protocol.

SEA Preparation
SEA of S. japonicum cercariae was obtained from Jiangsu Institute of Parasitic Diseases (China). SEA was sterile filtered, and endotoxin was removed with the use of polymyxin B agarose beads (Sigma). The Limulus amoebocyte lysate assay kit (Lonza, Switzerland) was used to confirm the removal of endotoxins from the SEA.

ELISA
The strips were coated with SEA overnight at 4°C and washed 3-5 times the next day. Then, the strips were covered with 10% FBS, 200 µL per well, for 1h. The plate was washed 3-5 times, and 100 µL of serum with the corresponding dilution ratio was added to each well and incubated at 37°C for 2h. The plate was washed 3-5 times, and HRP enzyme-labeled antibody was added at a corresponding dilution ratio of 100 µL in each well and incubated at 37°C for 1h. The plate was washed 5 times, 100 µL of substrate was added to each well and reacted for 5-30 mins, dilute sulfuric acid was added to terminate the reaction, and an enzyme label instrument was used to detect the results.

Statistics
Statistical analysis between different groups was performed using unpaired t tests. The software packages GraphPad Prism version 5.0a and SPSS Statistics 17.0 were used. P < 0.05 was considered statistically significant.

Infection-Induced Hepatitis
To explore the role of TLR7 in mice infected with S. japonicum, the survival rates of wild-type (WT) and TLR7 knockout (KO) mice infected with S. japonicum were recorded. As shown in Figure 1A, The dead mice were dissected, and gross changes in the liver in naive WT and TLR7 KO mice before and after infection were observed (C). Haematoxylin and eosin staining was performed on paraffin sections of liver tissue from infected WT and TLR7 KO mice. Liver tissue sections from naive mice were used as controls (D). The ratio of liver to body weight in WT and TLR7 KO mice before and after infection was calculated (E). Peripheral white blood cells were counted (F), and the levels of glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminase (AST) in serum were detected (G). Data were obtained from three independent experiments with 6-8 mice in each group, shown as the mean ± SEM. Statistical significance was determined by Student's t test, "ns" means no sense, *P < 0.05, **P < 0.01, and ***P < 0.001.  Figure 1B). In summary, this evidence indicated that TLR7 KO mice were susceptible to death during the acute infection stage.
The mice that died naturally during infection were dissected, and the livers were weighed. The proportion of the liver weight in mice was approximately 10%, which was 2-fold that in naive mice(Supplemental Figure 1). Because the liver is the target organ during S. japonicum infection, the proportion of the liver in mice was used as an index of infection conditions. Two groups of S. japonicum-infected WT and TLR7 KO mice were sacrificed at 42 d postinfection. The proportion of the liver weight in WT mice was 7.94%, while it significantly increased to 10.13% in TLR7 KO mice, indicating that the condition of TLR7 KO mice was more serious than that of WT mice ( Figure 1E).
To further investigate the difference between WT and TLR7 KO mice, HE sections of the liver were made 5-6 weeks after infection to analyse the pathological condition. Many white spots could be seen on the surface of WT livers, while the surface of TLR7 KO livers was smooth, with rare white spots ( Figure 1C). A large number of granulomas surrounding the egg were observed in the liver tissue of infected WT mice, but few granulomas were found in infected TLR7 KO liver tissue, in which there were many nude eggs, indicating poor granuloma reaction in TLR7 KO mice ( Figure 1D).
To further elucidate the roles of TLR7 in S. japonicum infection-induced hepatitis, blood was collected from both normal and infected WT and TLR7 KO mice, and serum aminotransferase (ALT and AST) and white blood cells (WBCs) were detected. The results indicated that the levels of WBC, ALT and AST increased significantly after infection (P < 0.05). Compared to the infected WT mice, the counts of WBCs in the blood and the levels of ALT and AST were lower in the serum of infected TLR7 KO mice (P < 0.05, Figures 1F, G). These results suggested that TLR7 enhanced S. japonicum infectioninduced inflammation in the body.

Cellular Distribution of TLR7 in the Livers of Infected Mice
To investigate the role of TLR7 during S. japonicum infection, we infected C57BL/6 mice with 40 ± 5 cercarias. After 5-6 weeks, the infected mice were sacrificed. Lymphocytes were isolated from the liver, and the expression of the TLR7 gene was identified by fluorescence quantitative PCR. As shown in Figure 2A, the expression of the TLR7 gene in lymphocytes from infected mice (88.06 ± 3.17%) was significantly decreased compared with that in lymphocytes from naive mice (P < 0.05). Furthermore, intracellular cytokine staining was performed to detect the proportion of TLR7expressing lymphocytes in the liver. The results indicated that the proportion of TLR7-positive cells was markedly increased 5-6 weeks post infection (P < 0.05) and was more than 2-fold upregulated in infected mice ( Figures 2B, C). This result suggested that TLR7 might modulate S. japonicum infectioninduced hepatitis. Moreover, to explore the distribution of TLR7 in the isolated hepatic lymphocytes, TLR7-positive cells were gated first. The percentages of MHCII + CD11C + DCs, CD19 + B cells, and CD11b + F4/80 + macrophages were detected by FACS. As shown in Figures 2D, E, the percentage of MHCII + CD11C + DC cells decreased significantly (P < 0.05), while the proportion of B cells did not change significantly, the percentage of CD11b + F4/80 + macrophages markedly increased in TLR7 + hepatic lymphocytes in the infected mice (P < 0.05), and most TLR7-expressing cells in the livers of infected mice were macrophages. This result indicated that TLR7 mainly established its role through macrophages in the livers of S. japonicum-infected mice. Moreover, MHCII + CD11C + DCs, CD11b + F4/80 + macrophages and CD19 + B cells were gated first, and the expression of TLR7 was detected by FACs. As shown in Figures 2F-H, the percentage of TLR7 + cells in macrophages increased significantly, which was consistent with the above results.

TLR7 Modulated the Responses of Hepatic Macrophages
To further explore the role of TLR7 in modulating the immune response of macrophages in the livers of S. japonicum-infected mice, hepatic lymphocytes were isolated from both naive and infected WT and TLR7 KO mice 5-6 weeks after infection. The percentage of F4/80 + CD11b + macrophages was first compared. As shown in Figures 3A, B, the results indicated that although the percentage of macrophages was not increased significantly in the liver after S. japonicum infection (P > 0.05), the absolute numbers of macrophages in the liver markedly increased (P < 0.05). This result suggested that macrophages were involved in S. japonicum infection-induced hepatic inflammation. However, the percentage of macrophages in the livers of infected TLR7 KO mice was higher than that in the livers of infected WT mice (P < 0.05), and there was no significant difference in the absolute number of macrophages between infected WT and infected TLR7 KO mice (P > 0.05). This implied that TLR7 did not directly affect the expansion of hepatic macrophages.
In addition, the expression of costimulators CD80 and CD86 was detected on the surface of macrophages. As shown in Figures 3A, C, the results indicated that the expression of CD86 in infected mice significantly increased compared to that in naive mice (P < 0.05), and the expression of CD86 in infected TLR7 KO mice was significantly lower than that in infected WT mice (P < 0.05). However, the changes in the expression of CD80 were not significant, either before and after infection or between WT-infected and KO mice (P > 0.05).
Moreover, cells were stimulated by PMA and ionomycin, and the secretion of IL-6, IL-12, IL-10, and IL-4 was also detected by intracellular cytokine staining. As shown in Figures 3D, E, the results indicated that the secretion of these cytokines was increased significantly, especially IL-4, which increased from 10% to approximately 50% (P < 0.05). Compared with infected WT mice, infected KO mice exhibited significantly reduced secretion of these cytokines, except for IL-6 (P < 0.05).

TLR7 Knockout Inhibited the Immune Response of Hepatic DCs
DCs are potent APCs that activate naive T cells. The counts of MHCII + CD11C + DCs in hepatic lymphocytes isolated from both naive and infected WT and TLR7 KO mice 5-6 weeks after infection were compared. As shown in Figures 4A, B, regardless of the proportion or absolute number, the DCs from infected TLR7 KO mice decreased significantly compared with those from infected FIGURE 2 | The distribution of TLR7 in APCs in the liver. WT mice were infected with S. japonicum, and livers were removed. Single-cell suspensions of liver were isolated from naive and infected mice. Total RNA of liver tissues from both naive and infected WT mice was extracted, cDNA was synthesized, and fluorescence quantitative PCR was performed to identify the expression of the TLR7 gene. The results are expressed as the percentage of gene expression relative to the same genotype (A). Representative graphs and statistical analyses of the percentages of TLR7 + cells in the isolated lymphocytes were detected by FACS (B, C). Representative graphs of DCs (CD11c + MHCII + ), macrophages (F4/80 + CD11b + ) and B cells (CD3 -CD19 + ) isolated from liver immune cells of WT and infected mice are shown (D, E). The proportion of DCs to TLR7 + cells in normal and infected mice was statistically analysed, and the proportion of macrophages and B cells was also counted. The expression of TLR7 in DCs, macrophages and B cells was also analysed. Representative graphs and statistical analyses are shown (F-H). Data were obtained from three independent experiments with 6-8 mice in each group, shown as the mean ± SEM. Statistical significance was determined by Student's t test, "ns" means no sense, *P < 0.05, **P < 0.01, and ***P < 0.001.
WT mice (P < 0.05). This result indicated that hepatic DCs might play an important role in the course of S. japonicum infection. More interestingly, the proportion of DCs in the livers of infected TLR7 KO mice was only 1.43%, which was significantly lower than that in the other groups (3%-6%) (P < 0.05). There was no significant difference in the absolute number of DCs in KO mice before and after infection (P > 0.05). This implied that TLR7 might be involved in the expansion of DCs in the course of S. japonicum infection. Although the CD86 in infected mice significantly increased compared to naive mice (P < 0.05), the expression of CD80 and CD86 was not significantly different between infected WT mice and infected TLR7 KO mice ( Figures 4A, C). The results of A C E B D FIGURE 3 | The function of liver macrophages during S. japonicum infection. WT and TLR7 KO mice were infected by S. japonicum. Five to six weeks later, livers were dissected out. Single-cell suspensions were separated from WT and KO mice before and after infection and stained with monoclonal antibodies against mouse F4/80, CD11b, CD80, and CD86. The expression of IL-4, IL-6, IL-10 and IL-12 was detected by intracellular cytokine staining. The strategy of gating macrophages is shown, and representative graphs of the expression of CD80 and CD86 in macrophages from WT and KO mice before and after infection are shown (A). The percentage and absolute number of macrophages were compared between WT and KO mice before and after infection (B). The expression of CD80 and CD80 was statistically analysed (C). Gated on macrophages, the expression of IL-4, IL-6, IL-10 and IL-12 was detected. Representative graphs and statistical analyses are shown (D, E). Data were obtained from three independent experiments with 6-8 mice in each group, shown as the mean ± SEM. Statistical significance was determined by Student's t test, "ns" means no sense, *P < 0.05, **P < 0.01, and ***P < 0.001. cytokine secretion showed that although the percentages of IL-4-, IL-10-, and IL-12-secreting DCs from WT mice increased significantly after infection (P < 0.05), no marked difference was found between infected WT and infected TLR7 KO mice (P > 0.05, Figures 4D, E).

TLR7 Enhances the Th2 Immune Response in the Course of S. japonicum Infection
The next step is to investigate whether these changes depend on the effects on T lymphocytes, surface markers and secretory A C B D E FIGURE 4 | The function of liver DCs during S. japonicum infection. WT and TLR7 KO mice were infected by S. japonicum. Five to six weeks later, livers were dissected out. Single-cell suspensions were separated from WT and KO mice before and after infection and stained with monoclonal antibodies against mouse MHCII, CD11c, CD80, and CD86. The expression of IL-4, IL-6, IL-10 and IL-12 was detected by intracellular cytokine staining. The strategy of gating DCs is shown, and representative graphs of the expression of CD80 and CD86 in DCs from WT and KO mice before and after infection are shown (A). The percentage and absolute number of DCs were compared between WT and KO mice before and after infection (B). The expression of CD80 and CD80 was statistically analysed (C). Gated on DCs, the expression of IL-4, IL-6, IL-10 and IL-12 was detected. Representative graphs and statistical analyses are shown (D, E). Data were obtained from three independent experiments with 6-8 mice in each group, shown as the mean ± SEM. Statistical significance was determined by Student's t test, "ns" means no sense, *P < 0.05, **P < 0.01, and ***P < 0.001. cytokines of T lymphocytes. After S. japonicum infection, liver T lymphocytes, mainly CD4 + T cells, increased (P < 0.05). Compared with infected WT mice, the total T lymphocytes of infected KO mice did not change significantly, but the decrease in CD4 + T cells was obvious (P < 0.05). Interestingly, the proportion of CD8 + T cells increased slightly (P < 0.05, Figures 5A, B). These results suggested that deletion of TLR7 might reduce the recruitment of CD4 + T cells. CD4 + T cells and CD8 + T cells from both WT and TLR7 KO mice expressed higher levels of activation-associated molecules after infection for 6 weeks (P < 0.05). The percentage of activated CD4 + T cells in TLR7 KO mice was significantly decreased compared to that in infected WT mice (P < 0.05). Only MHC II and CD69 were significantly decreased in CD8 + T cells from infected TLR7 KO mice compared to infected WT mice ( Figures 5C-F).
Furthermore, intracellular cytokine staining was performed on hepatic CD4 + T cells and CD8 + T cells isolated from different groups of mice. As shown in Figures 5I, J, most of the detected cytokines secreted by CD4 + T cells from infected WT mice were increased significantly compared to naive mice, such as IFN-g, IL-4, IL-2 and IL-6 (P < 0.05). IFN-g, IL-4, IL-2 and IL-6 secreted by CD4 + T cells from infected TLR7 KO mice significantly decreased compared to infected WT mice (P < 0.05, Figures 5G, H). A similar phenomenon has been observed in CD8 + T cells. The percentage of IFN-g-secreting CD8 + T cells increased significantly after infection (P < 0.05), while it decreased significantly in infected TLR7 KO mice, as well as IL-2 and IL-6 (P7 < 0.05).

TLR7 Enhanced the Hepatic B Cell Response in the Course of S. japonicum Infection
In the course of S. japonicum infection, B cells play an important role. The role of TLR7 in modulating the B cell response in the course of S. japonicum infection was also explored. As shown in Figures 6A, B, compared to naive mice, the proportion of CD19 + B cells in infected mice was decreased significantly (P < 0.05). The absolute numbers of CD19 + B cells increased significantly (P < 0.05). There was no change between uninfected and infected KO mice in the percentage of CD19 + B cells (P > 0.05).
Furthermore, activation-associated markers were detected on hepatic B cells from different groups of mice. As shown in Figures 6A, C, there was no difference in the proportion of B cells expressing CD69 and CD80 between WT-and KO-infected mice (P > 0.05), with only a significant decrease in the proportion of CD86 in the latter (P < 0.05).
In addition, SEA-specific antibodies were detected in the serum of mice ( Figure 6D). The levels of SEA-specific IgM and IgG antibodies in infected mice were significantly higher than those in naive mice (P < 0.05). Moreover, the levels of SEAspecific IgM and IgG antibodies in the serum of infected TLR7 KO mice were markedly decreased compared to those in infected WT mice (P < 0.05). These results suggested that TLR7 might play an important role in inducing antibody production in the course of S. japonicum infection.

DISCUSSION
TLR7 is an endosomal TLR that recognizes single-stranded RNA (ssRNA) and mediates the development of inflammation and autoimmunity (Luo et al., 2019;Souyris et al., 2019). In this study, both C57BL/6 mice (WT) and TLR7 KO mice were infected with S. japonicum. The survival results showed that the majority of infected mice did not die before 6 weeks post infection, while approximately half of TLR7 KO mice died within 6 weeks post infection, indicating that TLR7 may protect the host from death in the early phase of S. japonicum infection. S. japonicum ovulates eggs at approximately 4 weeks post infection, some of which deposit in the liver and cause granulomas to form around them (Pagan and Ramakrishnan, 2018). Once schistosomiasis progresses into the advanced phase, the host will always die from cirrhosis of the liver caused by granuloma inflammation (Malta et al., 2021). Reduced granulomatous formation did not improve host status and resulted in significant mortality, indicating that granulomatous inflammation can lead to pathological changes; on the other hand, granulomatous inflammation limits the spread of infection and plays a role in host protection (Hams et al., 2013). It is hypothesized that the death of TLR7 KO mice in the early phase of S. japonicum infection may be related to the absence of granulomas around the eggs. Therefore, TLR7 KO mice that died within 6 weeks post infection were dissected, and the livers were sectioned. There was poor granuloma formation in the liver of the host, and the liver tissue was filled with eggs of S. japonicum, which suggests that the deletion of TLR7 reduces the formation of granulomas during infection.
Next, the expression of TLR7 in hepatic lymphocytes was investigated. The results indicated that the relative TLR7 mRNA level in hepatic lymphocytes from infected mice was lower than that in hepatic lymphocytes from naive mice. This might be induced by the amount of infection, recruiting lower TLR7expressing lymphocytes, such as T cells and B cells, as we observed in mesenteric lymph nodes (Qu et al., 2019). This hypertension was validated by the FCM results, which indicated that the percentage of TLR7-expressing cells was increased in the isolated hepatic lymphocytes. Macrophages, DCs, and B cells are classic APCs that express many kinds of TLRs, including TLR7, and modulate the adaptive immune response (Assier et al., 2007). FCM results showed that in the isolated hepatic lymphocytes, most TLR7-expressing cells were CD11b + F4/80 + macrophages. On the other hand, the percentage of TLR7 + macrophages increased significantly in the livers of S. japonicum-infected mice. These results implied that TLR7 might modulate the S. japonicum infection-induced immune response mainly through macrophages. It was reported that activated macrophages (AAM) induced early in the anti-helminth response could amplify the early type 2 immune cascade initiated by epithelial cells and ILC2s, and subsequently driving parasite expulsion (Coakley and Harris, 2020). It suggested TLR7 triggering responses in liver resident macrophages mainly influenced the subsequent immune response in the liver of S. japonicum infected mouse. FIGURE 5 | The activation and function of liver CD4 + T lymphocytes and CD8 + T lymphocytes during S. japonicum infection. WT and TLR7 KO mice were infected by S. japonicum. Five to six weeks later, livers were dissected out. Single-cell suspensions were separated from WT and KO mice before and after infection and stained with monoclonal antibodies against mouse CD3, CD4, CD8, MHCII, CD69, CD62L and ICOS. Meanwhile, under stimulation with PMA plus ionomycin, the expression of IL-2, IL-4, IL-6, IFN-g, IL-10, IL-13, IL-17 and IL-21 was detected by intracellular cytokine staining. Representative graphs and the proportions of CD3 + , CD4 + and CD8 + T cells are shown (A, B). Then, CD4 + and CD8 + T cells were gated. The expression of MHCII, CD69, CD62L and ICOS was examined. Representative graphs and statistical analyses of CD4 + and CD8 + T cells in WT and KO mice before and after infection are shown (C-F). The expression of the abovementioned intracellular cytokines was also detected by flow cytometry. Representative graphs and statistical analyses of CD4 + and CD8 + T cells in both naive and infected WT and KO mice (G-J). Data were obtained from three independent experiments with 6-8 mice in each group, shown as the mean ± SEM. Statistical significance was determined by Student's t test, "ns" means no sense, *P < 0.05, **P < 0.01, and ***P < 0.001.
A C B D FIGURE 6 | The activation and function of liver B lymphocytes during S. japonicum infection. WT and TLR7 KO mice were infected by S. japonicum. Five to six weeks later, livers were picked out. Single-cell suspensions were separated from WT and KO mice before and after infection and stained with monoclonal antibodies against mouse CD69, CD80 and CD86. The strategy of gating B cells is shown, and representative graphs of the expression of CD69, CD80 and CD86 in B cells from WT and KO mice before and after infection are shown (A). The percentage and absolute number of B cells were compared between WT and KO mice before and after infection (B). The expression of CD69, CD80 and CD80 was statistically analysed (C). The SEA-specific antibody in mouse serum was detected by ELISA. The relative titres of anti-SEA IgG and anti-SEA IgM in the serum of WT and KO mice before and after infection were statistically analysed (D). Data were obtained from three independent experiments with 6-8 mice in each group, shown as the mean ± SEM. Statistical significance was determined by Student's t test, "ns" means no sense, *P < 0.05, **P < 0.01, and ***P < 0.001.
Liver-resident macrophages, also called Kupffer cells (KCs), are professional APCs that can modulate the class T cell response by providing costimulators and producing cytokines (Fu et al., 2020). CD80 and CD86 are important costimulators for T cell activation (Trzupek et al., 2020), and IL-12 and IL-4 are cytokines that induce Th2 and Th1 polarization, respectively (Cui et al., 2017). IL-6 and IL-10 are important proinflammatory cytokines and antiinflammatory cytokines, respectively (Kumar et al., 2021). Higher numbers of macrophages were found in the livers of infected WT and TLR7 KO mice, which expressed higher levels of CD86 and secreted more IL-4, IL-10, and IL-12, suggesting that macrophages played an important role in the progression of S. japonicum infection-induced hepatitis, as previously reported (Tan et al., 2019;Ye et al., 2020). Although the number of macrophages in the livers of infected TLR7 KO mice was similar to that in infected WT mice, they expressed lower CD86 levels and produced fewer cytokines. This result suggested that TLR7 is a main molecule that mediates the function of hepatic macrophages in the course of S. japonicum infection.
Dendritic cells are the only antigen-presenting cells that can activate naive T lymphocytes. It was reported that incomplete deletion of dendritic cells severely impairs the induction and development of the Th2 response (Phythian-Adams et al., 2010). A significant increase in the number of DCs in infected mouse livers, with higher CD86 expression and IL-4, IL-10 and IL-12 secretion, suggested that DCs were involved in S. japonicum infection-induced hepatitis. Moreover, the percentage and number of DCs in TLR7 KO mice at 6 weeks post infection significantly decreased compared to WT mice, and the percentage of IL-6-secreting DCs also decreased. This result suggested that the deletion of TLR7 significantly impaired the function of hepatic DCs, which could effect on the subsequent immune response in the liver of infected mouse, too.
The formation of granulomas in S. japonicum-infected mice depends on the Th2 response, and the deletion of T cells impaired granuloma formation around eggs . It was reported that TLR7 could enhance the type 2 immune response in many kinds of diseases (Chodisetti et al., 2020). In this study, the number and percentage of CD4 + T cells and the percentage of IL-4 + CD4 + Th cells in hepatic lymphocytes from infected TLR7 KO mice decreased significantly compared to those from infected WT mice. This result suggested that TLR7 plays an important role in enhancing the Th2 response during S. japonicum infection. Strangely, the secretion of IFN-g in TLR7 KO mice also significantly decreased compared to that in infected WT mice, which means that TLR7 modulates not only the Th2 response but also the Th1 response. Consistent with our results, administration of a TLR7 agonist was reported to induce a mixed Th1 and Th2 response in peripheral blood mononuclear cells in chickens (Annamalai et al., 2015).
In addition to differentiating into plasma cells to secrete antibodies, B cells are professional APCs that play important roles in the presentation of soluble antigens (Leon et al., 2019). Recently, reports showed that a subset of B lymphocytes can also activate naive T lymphocytes (Hong et al., 2018;Arroyo and Pepper, 2020). Higher numbers of B cells were found in the livers of infected WT mice expressing higher levels of CD69 and CD86, suggesting that hepatic B cells could mediate the T cell response in S. japonicum infection-induced hepatitis. In addition, these results implied that hepatic B cells could modulate the response of Th cells in turn. Recently, TLR7 was reported to be expressed on B cells and mediate the subsequent immune response Fillatreau et al., 2021). In this study, a higher percentage of B cells was found in TLR7-expressing hepatic lymphocytes, suggesting that TLR7 might directly mediate the immune response of B cells in the livers of infected mice. In addition, CD4 + Th2 cells are the main source of cells responsible for B cell activation and differentiation (Xiao et al., 2020). As mentioned before, TLR7 could enhance the response of Th2 cells in the livers of infected mice, and TLR7 could affect the responses of B cells through Th2 cells. Moreover, it was reported that APCs could help B cell activation via the formation of an immune synapse . This implied that APCs might be another pathway by which TLR7 regulates the S. japonicum infection-induced B cell response.
Altogether, this study indicated that TLR7 could delay the progression of S. japonicum infection-induced hepatitis by facilitating the formation of granulomas mainly through hepatic macrophages. DCs, B cells, and T cells are involved in TLR7-mediated immune responses.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

ETHICS STATEMENT
The animal study was reviewed and approved by Experimental Animal Ethics Committee of Guangzhou Medical University.

AUTHOR CONTRIBUTIONS
XP, XT, and JH conceived the study. YF, HX, and FS performed the in vitro cellular test. DC, AX, and JL performed histological experiment. CF and HW analysed the results. SY, HH, and HX prepared parasite and animal. JM, XW, and JH contributed to the writing of the paper. All authors contributed to the article and approved the submitted version.