Probiotic Cocktail Alleviates Intestinal Inflammation Through Improving Gut Microbiota and Metabolites in Colitis Mice

The modulation of the gut microbiome has been widely suggested as a promising therapeutic strategy for inflammatory bowel disease (IBD). Here, we established a novel probiotic cocktail to investigate its therapeutic role in acute colitis mice. During dextran sulfate sodium (DSS)-induced colitis, the mice were treated with the probiotic cocktail, fecal microbiota transplantation (FMT) from a healthy mice donor, or 5-aminosalicylic acid (5-ASA), respectively. The inflammatory responses were assessed by symptoms, serum inflammatory factors, and histological scoring. The intestinal barrier function was assessed by detecting tight junction proteins. Gut microbiota and its metabolites were further identified using 16S rDNA sequencing and a liquid chromatograph mass spectrometer (LC-MS/MS). Compared with FMT and 5-ASA treatment, the probiotic cocktail performed better in alleviating symptoms of colitis and decreasing disease activity score and mucosal inflammation. The probiotic cocktail also significantly decreased serum IL-17 level and increased JAM-1 expression in colon. The gut microbiota analysis confirmed that the beneficial effects of the probiotic cocktail were attributed to increasing anti-inflammatory bacteria Akkermansia, Bifidobacterium, and Blautia, while decreasing pro-inflammatory bacteria Parasutterella. The targeted metabolome analysis further indicated a rise in the production of Bifidobacterium-related short-chain fatty acids (SCFAs) such as propanoic acid and isobutyric acid after probiotics treatment. Taken together, the probiotic cocktail effectively alleviated intestinal inflammation through improving gut microbiota and metabolites in colitis mice, suggesting its great potential to be a novel therapeutic approach for IBD patients.


INTRODUCTION
Inflammatory bowel disease (IBD), characterized by chronic recurrent inflammation involving the whole colon (Wijmenga, 2005), is a major health concern worldwide with respect to the rising incidence in America and Asian countries during the past few decades (Thia et al., 2008;Kerur et al., 2021). Despite the great progress in anti-inflammatory, immunosuppressive (Hernańdez-Chirlaque et al., 2016), and biological agents, a considerable proportion of IBD patients continue to suffer from recurrence (Loddo and Romano, 2015). Therefore, the development of novel and safe therapeutic strategies for IBD is in critical need.
Emerging evidence suggests that gut microbiota dysbiosis contributes to the pathogenesis of IBD; for instance, opportunistic pathogen derived from ulcerative colitis patients displayed an inflammatory phenotype that caused mice colitis (Lloyd-Price et al., 2019;Seishima et al., 2019). Thus, the emerging methods of manipulating gut microbiota, such as probiotics and fecal bacteria transplantation (FMT), have been advocated as a promising strategy in alleviating intestinal inflammation. Burrello et al. reported that FMT treatment altered intestinal mucosal immunoreactive responses during experimental colitis, primarily by reducing colonic inflammation, increasing colonic barrier function, and simultaneously activating multiple immune-mediated pathways (Burrello et al., 2018). Meanwhile, both animal and clinical studies have demonstrated that the utilization of probiotics could prevent colonic inflammation via the regulation of intestinal macrophage differentiation, alternation of various inflammatory cytokines, and the enhancement of gut barrier Chen et al., 2021;Dias et al., 2021). The successful colonization of particular probiotics could also prevent pathogenic bacteria, interact with intestinal epithelium, and produce multi-functional beneficial metabolites (e.g., SCFAs and hydroxytryptamine) Stojanov et al., 2020). Nonetheless, the probiotics selected and analyzed in most cases were confined into one or several substrains, which cannot draw a consistent and convincing conclusion. Thus, we developed the probiotic cocktail strategy and evaluated the therapeutic potential of bacteria manipulation for IBD.
In this study, the probiotic cocktail contains 3 Bifidobacterium and 7 Lactobacillus substrains. Although numerous intestinal inflammation models have been developed to investigate IBD (Kiesler et al., 2015), here we utilized the dextran sulfate sodium (DSS)-induced colitis mice model to investigate the probiotic cocktail's therapeutic effects. DSS is a water-soluble sulfated polysaccharide that has been widely used to construct a colitis murine model (Okayasu et al., 1990) due to its simplicity, efficiency, and repeatability (Chassaing et al., 2014). Meanwhile, considering 5aminosalicylic acid (5-ASA) being the first-line clinical drug for the treatment of IBD patients (Magro et al., 2020), 5-ASA treatment together with healthy mice donor FMT were employed for comparison. Furthermore, microbial sequencing and targeted metabolomics were used to clarify its beneficial role on gut microbiota and metabolites in mice. Our study identified the colitis alleviation and the characteristics of gut microbiota and metabolites after the probiotic cocktail treatment, providing a novel probiotics-based therapeutic approach for IBD.
The animal protocols were approved by the Ethics Committee of Shanghai Tenth People's Hospital affiliated to Tongji University (SHDSYY-2018-KY0008).

FMT Preparation
(1) Twenty grams of fresh feces was collected from 25 healthy 6week C57BL/6 mice using anal massage, 5 consecutive days before the experiment; (2) the feces were immediately mixed with 100 ml of sterile 10% glycerite and PBS mixture after the collection to get the feces mixture; (3) the fecal microbiota suspension was obtained by grinding the feces mixture with a standard mortar and pestle; (4) to remove the insoluble impurities, the fecal microbiota suspension was filtered through screens with the following diameters: 0.4 mm, 0.2 mm, and 0.1 mm; and (5) the suspension was collected and stored at −80°C (Wong et al., 2017).

Assessment of Colonic Inflammation
Disease activity index (DAI) was used to assess the severity of colitis, including items of weight loss, stool consistency, and the presence of hematochezia, which were recorded every day during the experiment. DAI score calculation: (1) Bodyweight: 0 points were recorded if the bodyweight showed no decrease; 1 point was recorded when the bodyweight showed a 1%-5% decrease; 2 points were recorded when the bodyweight showed a 5%-10% decrease; 3 points were recorded when the bodyweight showed a 10%-15% decrease; and 4 points were recorded when the bodyweight showed more than 15% decrease. (2) Fecal traits: normal stool = 0 points; loose stool (not adhering to the anal paste of semi-formed stool) = 2 points; watery stool (can adhere to the anus watery stool) = 4 points. (3) Fecal occult blood result: no fecal occultation or naked eye blood stool = 0 points; fecal occult blood positive = 2 points; naked eye blood stool = 4 points. Finally, add the above three scores to get the DAI of each mouse to evaluate the severity of colitis (Tian et al., 2016;Li et al., 2019). The colon length and body weight alteration were also measured to evaluate the disease progression . We anesthetized mice with an inhalation anesthetic using 3% isoflurane during induction and 1.5% isoflurane during maintenance, then the serum was obtained by centrifuging blood using heart puncture drawn for 10 min at 3,000 rpm.

Real-Time Quantitative PCR Analysis
Total RNA was extracted using Trizol reagent (Invitrogen, USA, ref#15596018). An RT reagent kit (TAKARA, Japan, ref#RR047A) was used to reverse-transcribe RNA into cDNA. A TB Green qPCR kit (TAKARA, Japan, ref#RR420A) was used to carry out the real-time qPCR reactions. The process was controlled and the result was analyzed on the qPCR instrument (Roche, LightCycler ® 480II). All the experiments were performed in triplicate and b-Actin was selected as the reference gene for mRNA. The specific primer sequences (Occludin, ZO-1, JAM-1, and b-Actin) used for amplification are listed in Table 1.

Enzyme-Linked Immunosorbent Assay
The levels of cytokines (IL-4, IL-10, IL-17, IL-23, and IFN-g) in the serum of mice were measured with the corresponding ELISA kit (Simuwu-Biotechnology Co., Ltd., ref#SDM0006 96T, ref#SDM0010 96T, ref#SDM0012 96T, ref#SDM0117 96T, and ref#SDM0115 96T). The whole process was carried out according to the protocol. (1) Dilute the animal serum 10 times with diluent and then add 100 ml to each well, mix the reaction plate, and leave the plate at 37°C for 40 min; (2) wash the reaction plate 3 times with washing liquid, and invert the plate on a filter paper to dry it; (3) add distilled water and 50 ml of the first antibody working liquid to each well (except blank), thoroughly mix the reaction plate, and place the plate at 37°C for 20 min; (4) wash the reaction plate 3 times with washing liquid as described before; (5) add 100 ml of enzyme-labeled antibody working solution to each well and place the reaction plate for 10 min at 37°C; (6) add 100 ml of the substrate working solution to each well and place the plate at 37°C in the dark for 15 min for reaction; (7) add 100 ml of the stop solution to each well and mix them well; (8) measure the absorbance value at 450 nm with a microplate reader (Tecan, F50) within 30 min.

16S rDNA Sequencing
The fecal samples were prepared according to the manufacturer's instructions and the DNA was extracted from fecal samples as previously described (Kong et al., 2021a). A Nanodrop 2000 UVvis spectrophotometer (Thermo Scientific, Wilmington, MA, USA) and 1% agarose gel electrophoresis were utilized to TABLE 1 | Primer sequences used in this study.

Hematoxylin-Eosin Staining and Histopathology Evaluation
Paraffin-embedded colon tissues were cut into 5-mm sections. After being dewaxed in xylene and dehydrated in gradient alcohol, the sections were stained with hematoxylin for 8 min and with eosin for 5 min. Then, the sections were dehydrated and sealed. The histopathology evaluation was performed by two researchers who are blinded to section information. The following evaluation criteria were used: Epithelium (E): 0, normal morphology; 1, loss of goblet cells; 2, loss of goblet cells in large areas; 3, loss of crypts; 4, loss of crypts in large areas. Infiltration (I): 0, no infiltrate; 1, infiltrate around crypt basis; 2, infiltrate reaching to L. muscularis mucosae; 3, extensive infiltration reaching the L. muscularis mucosae and thickening of mucosa with abundant edema; 4, infiltration of the L. submucosa. The total histological score is defined as the sum of the epithelium and infiltration score (total score = E + I) (Obermeier et al., 1999).

Metabolomics
The feces samples were prepared for metabolomic analysis according to the manufacturer's instructions (Metabo-Profile Biotechnology, Shanghai). An ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system (ACQUITY UPLC-Xevo TQ-S, Waters Corp., Milford, MA, USA) was used to quantitate the 7 main kinds of SCFAs (propanoic acid, isovaleric acid, isobutyric acid, butyric acid, valeric acid, hexanoic acid, and acetic acid) in this project as previously described . The raw data files generated by UPLC-MS/MS were processed using the MassLynx software (v4.1, Waters, Milford, MA, USA) to perform peak integration, calibration, and quantitation for each metabolite. Statistical analysis was conducted in Prism 8 (GraphPad) and R (v3.5.1).

Statistical Analysis
All data were expressed as mean ± standard deviation (SD). The SPSS 22.0 software (SPSS Inc., Chicago, IL, USA) was utilized for statistical analyses. The Mann-Whitney U test and one-way ANOVA test were used to identify the significant differences between groups. Mann-Whitney U test and Pearson's chi-square were utilized to identify the continuous and categorical variables, respectively. p < 0.05 was considered statistically significant.

The Probiotic Cocktail Alleviates Intestinal Inflammation in DSS-Induced Colitis Mice
As shown in Figures 1A, B, compared to the control group, the probiotic cocktail treatment significantly slowed down the weight loss of colitis mice, whereas the body weight among the FMT, 5-ASA, and control groups had no statistical difference. In evaluation of the severity of colitis, the DAI scores were found to significantly decrease in the probiotic cocktail, ASA, and FMT groups ( Figures 1C, D), with the probiotic cocktail group obtaining the lowest score ( Figures 1C, D). Furthermore, the probiotic cocktail notably increased the colon length of colitis mice compared to the control and ASA groups ( Figures 1E, F). The images of representative hematoxylin-eosin (H&E) staining for colon and histological evaluation ( Figures 2G-L) indicated that the probiotic cocktail performed best in alleviating the mucosal inflammation of DSS-induced colitis mice than any other treatment (red arrows represent enriched inflammatory cell and crypt for the probiotics group).

The Probiotic Cocktail Reduces the Level of Serum Inflammatory Cytokines and Upregulates Tight Junction Proteins in DSS-Induced Colitis Mice
To further clarify the anti-inflammatory effect of the probiotic cocktail, we detected several inflammatory cytokines in the serum samples from colitis mice. As shown in Figures 2A-E, compared with the control group, the probiotic cocktail, FMT, and 5-ASA groups significantly decreased the level of proinflammatory IFN-g and IL-17. The trend of decreased proinflammatory IL-23 and increased anti-inflammatory IL-10 and IL-4 was also observed in colitis mice after treatment of probiotic cocktail, although there was no significant difference ( Figures 2C-E). Of note, the probiotic cocktail had a better inhibitory effect in serum IFN-g and IL-17 production than FMT and 5-ASA (Figures 2A, B). Since our previous study revealed that probiotics played a crucial role in protecting the intestinal barrier (Liu et al., 2014;Yin et al., 2018), we also detected the expression of several tight junction proteins (JAM-1, ZO-1, and Occludin) in the colon tissues from colitis mice. As shown in Figures 2F-H, the probiotic cocktail significantly increased the mRNA expression of ZO-1 as compared with the control group, and the upward trend was also observed in JAM-1 and Occludin although not significant. Meanwhile, the following Western blot confirmed the upregulation of ZO-1, JAM-1, and Occludin at the protein level in the probiotic cocktail group ( Figure 2I).

The Probiotic Cocktail Improves the Gut Microbiota of DSS-Induced Colitis Mice
To investigate the altered gut microbiota after different treatments, 16S rDNA sequencing was performed. Alpha diversity indexes were calculated to assess the differences in bacterial diversity among groups. Compared to the control group, the FMT group had a significantly higher Chao1, Shannon, and Simpson index (all p < 0.05), which was opposite for the probiotics group ( Figures 3A-C). This finding suggested that the number and abundance of other species decreased after the probiotics were dominant. Meanwhile, the principal coordinate analysis based on weighted UniFrac distance revealed the significant differentiation in the composition of gut microbiota among groups ( Figure 3D). At the phylum level, the relative abundance of Verrucomicrobia was increased in the probiotics group but decreased in the FMT and 5-ASA groups (Control: 28.99%, Probiotics: 42.95%, FMT: 22.48%, ASA: 15.94%, Blank: 38.66%), while Firmicutes changed in an opposite manner (Control: 13.98%, Probiotics: 8.78%, FMT: 31.41%, ASA: 32.12%, Blank: 39.08%) ( Figure 3E). In addition, the relative abundance of Proteobacteria was decreased in the probiotics and FMT groups as compared with that of the control group (Control: 18.66%, Probiotics: 9.78%, FMT: 5.69%, ASA: 18.85%, Blank: 0.91%). At the genus level, the relative abundance of Akkermansia and Bifidobacterium was increased in the probiotics group but decreased in the ASA group (Control: 31.98%, Probiotics: with H&E from each group. One-way ANOVA test was utilized, and data were shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. DSS, dextran sodium sulfate; PBS, phosphate buffered saline; FMT, fecal microbiota transplantation; 5-ASA, 5-aminosalicylic acid; DAI, disease activity index; HDI, histological damage index.  Figure 3F). Furthermore, LEfSe analysis was utilized to figure out the key elements among different groups. At the genus level, DSS treatment significantly reduced the beneficial bacteria including Bifidobacterium and Blautia, which were reported to inhibit inflammation and enhance gut barrier . Notably, these two genera were obviously upregulated after the probiotic cocktail treatment. Moreover, we observed that the key genera in the FMT group were Dorea, Oscillibacter, Desulfovibrio, Butyricicoccus, Micipirllum, Intestinimonas, Clostridium IV, and XIVb, whereas the dominant bacteria in the ASA group were Escherichia_Shigella, Clostridium sensu stricto, and Romboustia ( Figures 4A, B).

The Probiotic Cocktail Promotes the Production of Beneficial Bacterial SCFAs
Emerging evidence suggests that gut microbiota exerts an antiinflammatory effect through producing SCFAs. Accordingly, we performed targeted metabolomics to detect the levels of several SCFAs (propionic acid, isovaleric acid, isobutyric acid, butyric acid, valeric acid, hexanoic acid, and acetic acid) in the fecal samples of DSS-induced colitis mice (Figures 5A-G). Compared with the control group, the probiotic cocktail rather than FMT and 5-ASA significantly promoted the production of propanoic acid and isobutyric acid (Figures 5A, C). The upward trend of isovaleric acid, butyric acid, valeric acid, hexanoic acid, and acetic acid was also observed in the probiotic cocktail group One-way ANOVA test was utilized, and data were shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, no significant difference. DSS, dextran sodium sulfate; PBS, phosphate buffered saline; FMT, fecal microbiota transplantation; 5-ASA, 5-aminosalicylic acid.
though not significant (Figures 5B, D-G). Additionally, Spearman rank correlation analysis was used to test correlations between bacteria and SCFA abundance ( Figure 5H). Unsurprisingly, butyrate-producing bacteria Bifidobacterium and Roseburia were positively correlated with most SCFAs, while it was opposite for some pro-inflammatory bacteria such as Parasutterella and Bacterioides. Taken together, the results suggested that the probiotic cocktail effectively promoted the accumulation of both butyrate-producing probiotics and SCFAs.

DISCUSSION
In this study, we investigated the anti-inflammatory role of a novel probiotic cocktail in DSS-induced acute colitis mice. Remarkable differences are observed among healthy mice donor FMT, 5-ASA, and probiotics groups. Notably, probiotics treatment demonstrated its great value in preventing weight loss and intestinal contracture, upregulating SCFA-producing bacteria along with SCFA levels, and decreasing DAI score, pro-inflammatory factors, and intestinal barrier markers. These findings highlight that the probiotic cocktail could represent a promising dietary therapeutic strategy for IBD ( Figure 6).
Exploring the mechanism revealed that probiotics intervention not only decreased the level of pro-inflammatory cytokines, namely, IFN-g, IL-17, and IL-23, but also increased the anti-inflammatory cytokines IL-10 and IL-4, which may be mainly due to the successful colonization of both Bifidobacterium and Lactobacillus. Consistently, Bo et al. reported that Bifidobacterium significantly reduced IFN-g and improved IL-10, alleviating enterotoxigenic Escherichia coli-induced diarrhea and can be a potential probiotic for clinical therapy . Yue et al. found that enterotoxigenic E. coli-induced diarrhea can also be suppressed by orally given Lactobacillus plantarum via regulating IFN-g, IL-6, and IL-10; alternating gut microbes; and increasing gut SCFAs (Yue et al., 2020).
The intestinal barrier mainly consists of 3 kinds of barriers: (1) a physical barrier composed of tight epithelial junctions; (2) a secretory barrier composed of antimicrobial peptides and mucus; The concentration of 7 main kinds of SCFAs (propanoic acid, isovaleric acid, isobutyric acid, butyric acid, valeric acid, hexanoic acid, and acetic acid) in the feces of blank mice, and DSS-induced colitis mice treated with PBS, healthy mice donor FMT, 5-ASA, or the probiotic cocktail. One-way ANOVA test was utilized, and data were shown as mean ± SD. (H) The Spearman correlation analysis illustrated the relationship of SCFAs and gut microbiota. +p < 0.05; * p < 0.01. DSS, dextran sodium sulfate; PBS, phosphate buffered saline; FMT, fecal microbiota transplantation; 5-ASA, 5-aminosalicylic acid; SCFAs, short-chain fatty acids. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. and (3) an immune barrier composed of immune cells or immune molecules (Camara-Lemarroy et al., 2018), which gets damaged under colitis conditions (Ungaro et al., 2017). Tight junction molecules are often used to evaluate intestinal barrier function in colitis (Furuse et al., 1993;Miner-Williams and Moughan, 2016;Mirsepasi-Lauridsen et al., 2019). Also, our results showed that probiotics increased the expression of intestinal mucosal tight junction proteins, represented by JAM-1, ZO-1, and Occludin, at both transcription and protein levels. These markers indicated the recovery of the gut barrier and the alleviation of colitis symptoms, which may be attributed to the effect of Lactobacillus and Bifidobacterium. As supported by a systematic review, Lactobacillus and Bifidobacterium exerted the anti-inflammation effect mainly through the enhancement of tight junction function in colorectal cancer patients (Wierzbicka et al., 2021). In addition, researchers found that the application of Lactobacillus and Bifidobacterium restored the gut barrier and defended gut-derived pathogenic infections in neonatal rats and dinitrobenzene sulfonic acid (DNBS)-induced colitis mice (Martıń et al., 2016;Zeng et al., 2017). Moreover, the microbiome and metabolome analysis indicated that probiotics treatment significantly improved the gut microbiota and promoted the production of bacteria-derived SCFAs. The microbiome composition displayed remarkable changes with the administration of the probiotic cocktail in this study, represented by increased Akkermansia, Bifidobacterium, and Blautia, and decreased Parasutterella. Akkermansia is a Gram-negative anaerobic bacterium that is reported to be selectively decreased in the fecal microbiome of IBD patients (Pittayanon et al., 2020). Akkermansia was also found to improve colitis-associated colorectal cancer and colitis through the alternation of cytotoxic T lymphocytes (CTLs), represented by CD8+ CTLs, CD16/32+ macrophages, and PD-1+ CTLs . Moreover, our previous research showed that ketogenic diets improve colitis mice through enriching Akkermansia, enhancing intestinal barrier function, and reducing the production of group 3 innate lymphoid cells and associated inflammatory cytokines, which highlights the importance of our finding that Akkermansia was increased in the probiotics group (Kong et al., 2021a). Meanwhile, Bifidobacterium and Blautia were reported to alter gut microbiome dysbiosis, produce SCFAs, improve gut barrier function, block related pro-inflammatory cytokines, modulate T regulatory cells, and collectively blunt colitis in animal models (Kong et al., 2019;Sun S et al., 2020;Chen et al., 2021;Engevik et al., 2021;Song et al., 2021;Yang et al., 2021). However, Parasutterella, as a harmful bacterium that increased with the growth of age , was found decreased after probiotics treatment. Parasutterella was also a key potentially pathogenic bacterium that is intensely associated with the progress of ulcerative colitis . Lactobacillus, an SCFA-producing bacterium, was expected to increase but failed to populate during our research, probably due to its poor ability to colonize and the limited intervention time. However, its contribution can be seen through the alternation of Akkermansia and associated beneficial bacteria, which follows previous studies Zhang et al., 2021).
Accumulating lines of evidence indicate that the intervention of probiotics makes it work through the alternation of fecal metabolites, especially SCFAs (Kong Q et al., 2021;Zhang et al., 2021). Thus, we performed the metabolome analysis using the fecal samples of colitis mice. Compared with the blank group, we found that DSS treatment significantly reduced the abundance of SCFAs, which could be reversed by the probiotics supplement, especially the upregulated propanoic acid and isobutyric acid, but not by healthy mice donor FMT and 5-ASA treatments. Clinical and basic studies confirm that microbiota-derived propionate acts directly on intestinal gd T cells, inhibiting their production of IL-17 and IL-22 mainly through a histone deacetylase-dependent manner (Dupraz et al., 2021). Moreover, animal and human studies found that a similar increase in SCFAs protected liver function, reduced intestinal inflammation, and protected against colorectal cancer through regulating CRP level and hepatic lipid metabolism, decreasing proinflammatory Th1 and Th17 cells, and increasing antiinflammatory immune cells (Alrafas et al., 2020;Ziętek et al., 2021). Furthermore, LeBlanc et al. claimed that Bifidobacterium produced acetate and formate in limited carbohydrate FIGURE 6 | Schematic summary of the probiotic cocktail for alleviating acute intestinal inflammation. During the process of acute inflammation (left part), harmful bacteria may attach to the injured intestinal epithelium, inhibiting commensal microbes to colonize and make function, destroying the gut barrier and tight junction proteins by regulating pro-or anti-inflammatory factors. After probiotics intervention (middle part), colonized butyrateproducing probiotics Bifidobacterium, together with the associated SCFAs, alleviated the progression of acute intestinal inflammation through enhancing the intestinal barrier, regulating the muti-inflammatory factors, and expelling potential pathogenic bacteria. In the normal colon (right part), balanced commensal microbes interacted with the intestinal mucosa, contributing to the dynamic balance of host health.
conditions, while supplying with carbohydrate could reproduce acetate and lactate (LeBlanc et al., 2017). Juneyoung et al. found that the transplantation of SCFA-producing bacteria represented by Bifidobacterium and Lactobacillus could alleviate neurological deficits and inflammation after stroke, and elevated SCFA concentrations of gut, brain, and plasma in aged stroke mice . In Spearman correlation analysis, beneficial bacteria including Ruminococcus, Bifidobacterium, and Allobaculum were significantly positively related to SCFA abundance, which was consistent with previous studies (Kong et al., 2019;Lee et al., 2020;Wang et al., 2020;Song et al., 2021). To sum up, the increased beneficial bacteria and their related metabolites (SCFAs) might be able to explain the mechanism by which probiotics alleviated the clinical and molecular changes associated with colitis.
These findings collectively supported the promising application of the probiotic cocktail in clinical practice. Despite our encouraging findings, there are several limitations that should be improved in our future work. Firstly, our work was only based on an animal model, and the actual therapeutic role of the probiotic cocktail in IBD patients remains undiscovered. This limitation is hoped to be solved by well-designed clinical trials based on sufficient patient resources. Secondly, the optimal working dose of the probiotic cocktail should be determined and more information on the affected microbiome should be provided by metagenomics sequencing instead of 16S rDNA sequencing. Finally, the molecular mechanisms of the probiotic cocktail in regulating barrier function and inflammatory cytokines still need to be further investigated in depth using in vitro and in vivo experiments.

CONCLUSION
Our study for the first time demonstrated that a novel probiotic cocktail can effectively alleviate intestinal inflammation in DSSinduced colitis mice. Probiotic cocktail intervention significantly decreased the level of pro-inflammatory cytokines, upregulated the expression of tight junction proteins, improved gut microbiota, and promoted the production of beneficial SCFAs. Although clinical validations are necessary, the probiotic cocktail has great potential to be developed as an effective therapeutic strategy for IBD patients.

DATA AVAILABILITY STATEMENT
The data presented in the study are deposited in https:// www.ncbi.nlm.nih.gov, accession number PRJNA819569.

ETHICS STATEMENT
The animal study was reviewed and approved by the Ethics Committee of Shanghai Tenth People's Hospital affiliated to Tongji University.

AUTHOR CONTRIBUTIONS
YFZ, YX, and XW contributed equally to this paper. YFZ and YX performed the experiments and drafted the manuscript. XW analyzed the data and helped with the polishing of the manuscript. LR and XY helped with the insightful discussions. YZ helped in attending to the mice. TS provided the probiotics and help in the gavage of mice. CK and LZ designed and supervised this study. All authors contributed to the article and approved the submitted version.