Getting clues from nature: the impact of grass hay on suckling piglets’ gastrointestinal growth and colonic microbiota

Introduction The effect of dietary fiber on pig production has been extensively evaluated. Inspired by observations of the diet of wild, young piglets, this study aimed to examine the possibility of feeding grass hay to suckling piglets besides concentrated creep feed. Methods The sow-nursed piglets in this study were divided into two groups based on balanced sow parities. The control group (CON, n = 7 sows) only received a regular, concentrated creep feed, while the treatment piglets (GH, n = 8 sows) were also provided with chopped grass hay from 2 days of age until weaning (28 days). At weaning, one piglet with a median weight was selected from each litter for post-mortem evaluation. Subsequently, six pigs around median weight per sow were grouped into nursery pens and monitored for their feed intake and body weight gain until 9 weeks of age. Results and discussion Piglets in GH consumed, on average, 57 g of grass hay per piglet during the entire lactation period. The emptied weight of the small and large intestine was significantly greater in GH (280 vs. 228 g, 88.8 vs. 79.3 g, respectively, p < 0.05), and the length of the large intestine was stimulated by the grass hay (164 vs. 150 cm, p < 0.05). Morphologically, the villus height in the jejunum was higher in GH (p < 0.05). In the large intestine, the crypt depth of the mid-colon was lower in GH. Moreover, the short-chain fatty acid (SCFA) concentrations in the cecum were increased in GH compared to CON (1,179 vs. 948 µmol/g dry matter, p < 0.05), whereas in the colon, SCFA concentrations were lower in CON (341 vs. 278 µmol/g dry matter, p < 0.05). There was no major impact of grass hay inclusion on the colonic microbiota composition. Only a trend was observed for a lower inverse of the classical Simpson (InvSimpon) index and a higher abundance of Lactobacillus genera in GH. After weaning, no significant differences in feed intake and body weight gain were observed. In conclusion, supplementing the grass hay to suckling piglets led to alterations in intestinal morphology, increased SCFA fermentation in proximal sections of large intestine, stimulation of gastrointestinal tract growth, and subtle modifications in colonic microbiota.


Introduction
The weaning transition is a challenge in swine breeding husbandry.To maximize profits, early-weaning strategies at 3-4 weeks of age have been widely adopted on commercial farms.Such sudden transition of diets predisposes piglets to diarrhea, aberrant behaviors, growth stasis, and even more detriments associated with weaning stressors, eventually leading to production loss (Lallès et al., 2007;Summers et al., 2019).To address these concerns, creep feed is provided to familiarize the piglets during the suckling period with the solid feed after weaning and partly release the pressure for the lactating sows (Miller et al., 2012;Novotni-Dankoé t al., 2015).
Although consumption of creep feed can lead to higher feed intake after weaning with concomitant greater growth performance (Bruininx et al., 2002;Pluske et al., 2007;Muns and Magowan, 2018), the application of creep feed still comes with variable feed intake, limited impact on the gut maturation during the preweaning period or the later life phases of piglets (Sulabo et al., 2010;Van den Brand et al., 2014).Meanwhile, the presence of maternal fecal semiochemicals in farrowing crates is also appealing to piglets (Aviles-Rosa et al., 2020), and gastrointestinal microbiota is primarily influenced by maternal and environmental factors (Lührmann et al., 2021;Nowland et al., 2021).Therefore, there is room to improve the early solid nutrition of piglets to activate their appetite and gut maturation.
In a semi-natural environment, piglets living outdoors already begin chewing straw and rooting during the second week of life (Schouten, 1985;Petersen, 1994).Our previous research on feral piglets showed spontaneous solid feeding intake from the first week of life, primarily leaves and stems, accounting for 83% of their stomach contents.This intake of fibrous plant matter was associated with a stronger stomach development (Van Hees et al., 2022).Compared to feral piglets, farm-raised piglets are usually only able to access creep feed formulated as being nutrient-dense and digestible.Therefore, the inclusion of fibrous content in creep feed is a promising avenue to explore, and it was reported to support the piglets' behavior, including recognition memory, and frequent exploration and interaction with littermates (Clouard et al., 2018;Fleming et al., 2019).Meanwhile, in terms of physiology, a study by our group showed that the addition of dietary insoluble fiber in creep feed activated the colonic fermentation of short-chain fatty acids (SCFAs) and increased large intestinal size and fill, which strengthened the health of the digestive tract (Van Hees et al., 2019).These studies included the fiber sources as a ground ingredient mixed in the creep feed, which is obviously different from the intake of structure-rich plant parts in the wild.Grass hay is rarely fed to farmed pigs, but a common part of the natural diet of wild boar (Groot Bruinderink et al., 1994).
Because of the above, we wanted to mimic a part of the natural piglet diet by providing grass hay to suckling piglets.The objective of this study was to investigate whether a grass hay supplement would stimulate the appetite of piglets and foster development of a stronger gastrointestinal tract from an early age, enabling them to cope with weaning stress and achieve better performance in later phases.

Materials and methods
The housing and rearing of experimental animals were in compliance with European Union Directive 2010/63/EU.The protocols and procedures of this study were approved by the Ethics Committee of Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), with application number 2022/420, and all animal experiments complied with the ARRIVE guidelines 2.0.

Animal housing and management
Fifteen sows (TN 70; average parity 3.9, from 1 to 8) were involved in this study.They were housed individually in the farrowing room of the research farm (ILVO, Melle, Belgium) 7 days prior to the expected farrowing date.The farrowing crates for sows were equipped with slatted floor including a heat lamp, a drinking nipple, a jute sack, and a feed trough that avoided the piglets consuming sow feed.The environmental temperature was controlled at approximately 23°C, and lighting was provided between 7:00 and 16:00 and dimmed during the night.The sows' diet was offered ad libitum and mainly based on wheat, barley, and maize containing 155 g of crude protein, 55 g of crude fiber, and 51 g of crude fat per kg during lactation.The day when most litters were born was defined as d0 of this experiment and the piglets were weighed immediately after birth and identified individually by ear tags individually.The litter size was standardized to 14.2 ± 1.0 piglets within 3 days after farrowing by cross-fostering within these 15 sows in terms of the available nipples of sows and birth weight of piglets.Surgical castration and teeth clipping were not applied.The sleeping area for the piglets was heated by infrared light and floor heating was provided from birth to day 14.All piglets were offered creep feed ad libitum from day 2 onwards and another water nipple for the piglets fixed to the farrowing crate is also available.Weaning took place at day 28.Then, the piglets were moved to the nursery units on day 63.The arrangement for nursery phase is described below.

Experimental design and treatments
Sows were allocated to one of two treatments based on balanced parities.Seven litters were designated as the control group (CON), and piglets in the control group received the common concentrated creep feed (Table 1) in a round feeder from day 2 to weaning (day 28).In the treated group (GH), piglets received the same creep feed, but additionally, chopped grass hay (particle size: 6.0 ± 1.0 cm; analyzed nutritional components: dry matter 93.1%, crude protein 7.7%, crude fat 1.0%, crude ash 7.1%, NDF 53.1%, ADF 26.7%) in a separate feeder until weaning.The positions of creep feeders were identical across all litters, and grass hay feeders in the GH group were positioned equivalently and adjacent to the creep feeder.All piglets had ad libitum access to creep feed or grass hay and water.The weight of all diets in the feeders was recorded and leftovers were replaced by fresh material and collected by a vacuum cleaner on a litter basis every morning.As weaning approached, the suckling piglets were gradually transitioned from creep feed to weaner diets starting at 7 days prior to weaning.
Upon weaning, piglets were moved to the nursery unit.Six piglets from the same litter with median weight and, as far as possible, balanced gender, were selected and assigned to one of the nursery pens (1.0 m × 2.0 m), resulting in 15 pens in total.All selected piglets only received common commercial nursery feed based on barley, corn, and wheat (consisting of 17.5% crude protein, 3.6% crude fiber, 5.0% crude fat, and 6.6% ash) after weaning and the remaining piglets were moved to other nursery pens to follow normal breeding procedures of this farm.The selected piglets had ad libitum access to nursery feed and drinking water and were monitored until 9 weeks of age (day 63).

Sampling and measurement
The individual body weight of piglets was measured at birth, on day 14, at weaning (day 28), and on day 42 and day 63.The disappearance of creep feed and grass hay was daily recorded to calculate the feed intake per litter.One day before weaning, one piglet per litter with a median body weight was selected for euthanasia by intra-cardiac injection with 30% barbiturate pentobarbital injection (Release, WDT Co. Germany) after sedation with 0.22 mL/kg Zoletil (Covertrus Co. USA).A midline laparotomy was performed to excise and separate each section of gastrointestinal tract.The full and emptied stomach, liver, and spleen were weighed.The small intestine (SI) and large intestine (LI) were ligated at their respective junctions, and their lengths were recorded on a dissection table while in a relaxed state.The SI and LI were then weighed both with and without contents.Additionally, 3cm2 tissue samples at the same middle position from duodenum, jejunum, ileum, and mid-colon were washed with phosphatebuffered saline (PBS) and collected into a 4% formaldehyde solution used for microscopic examination.The representative and homogenized digesta from cecum and mid-colon were collected on dry ice first and subsequently transferred and stored to −80°C until analysis.

Luminal contents' metabolic profile
The SCFAs of digesta were quantified according to the approach used by Gadeyne et al. (2016).Five milliliters of 10% formic acid containing the internal standard (1 mg of 2-ethyl butanoic acid) was added to 1 mL content, and after 15 min of centrifugation (22,000 g at 4°C), the supernatant was filtered and an aliquot was transferred into a 1.5-mL glass vial.The SCFAs were measured by gas chromatography (HP 7890A, Agilent Technologies, Diegem, Belgium), equipped with a flame ionization detector and a Supelco Nukol capillary column (30 m × 0.25 mm × 0.25 µm, Sigma-Aldrich, Diegem, Belgium).

Intestinal morphometry evaluation
Five-micrometer-thick sections of intestinal organs were cut from paraffin-embedded blocks (Sadeghipour and Babaheidarian, 2019) and then stained with hematoxylin-eosin (H&E) for lightmicroscopic examination (Leica DM LB2 with microscope imaging software from Leica Microsystems).For each slice, 10 intact villi or crypts (in mid-colon) of each slice were randomly selected and each sample had four slices serving as the replicates.The villus height and the depth of crypt adjacent to the selected villi were determined.The ratios between villus height and crypt depth were also calculated.

Mid-colon microbiota community analysis
To analyze the microbiota community, the representative luminal samples from mid-colon were taken and stored on dry ice and transferred to −80°C immediately.The microbial DNA in samples were extracted using PowerSoilPro (QiaGen, Germany) with a beat-beating step of 5 × 4,000 rpm for 15 s with 45-s intervals using a PowerLyzer instrument (QiaGen).Then, the DNA was eluted in 50 µL of elution buffer.Ten microliters of genomic DNA extract was sent out to LGC genomics GmbH (Berlin, Germany) where the 16S rRNA gene V3-V4 hypervariable region was amplified.The PCR mix included 1 µL of DNA extract, 15 pmol of both the forward primer 341F 5'-NNNNNNNNNTC CTACGGGNGGCWGCAG and reverse primer 785R 5'- (Klindworth et al., 2013) in 20 µL volume of MyTaq buffer containing 1.5 units of MyTaq DNA polymerase (Bioline) and 2 µL of BioStabII PCR Enhancer (Sigma).The reaction conditions were carried out for 30 cycles using the following parameters: 2 min at 96°C for predenaturation; 96°C for 15 s, 50°C for 30 s, and 70°C for 90 s.The DNA concentration of amplicons of interest was determined by gel electrophoresis.The amplicon pools were purified with one volume AMPure XP beads (Agencourt) to remove primer dimer and other small mispriming products, followed by an additional purification on MinElute columns (Qiagen, Germany).Illumina libraries were pooled and sizeselected by preparative gel electrophoresis.Sequencing was performed on an Illumina MiSeq using v3 Chemistry (Illumina).

Statistical analysis
Statistical analysis was performed using a general linear model to evaluate the effect of dietary treatment on growth performance and GIT characteristics with the litter at suckling phase and pen at post-weaning phase as the experimental unit by SPSS version 27.0 software (IBM SPSS Inc., USA).A covariate was retained when its p-value was less than 0.200 and not affected by treatment.Consequently, the birth weight to the growth performance and body weight at dissection were included as the covariates.For all analyses, we applied the Tukey-Kramer correction for post-hoc multiple comparison.Differences were considered significant if p < 0.05 while a tendency was considered when 0.05 < p < 0.100.
The DADA2 R package was used to process the amplicon sequence data according to the pipeline tutorial (Callahan et al., 2016).Finally, the amplicon sequence variant (ASV) table obtained after chimera removal was used for taxonomy assignment using the Naive Bayesian Classifier and the DADA2 formatted Silva v138 (Quast et al., 2013).

Results
Three piglets died within 3 days after treatment due to crushing and they were excluded from the results.Generally, according to daily monitoring, the animals in this study exhibited good clinical health throughout the experiment.

Growth performance
There were no significant differences in the results of weight gain between the groups until weaning, but when we categorized the piglets into three levels based on their birth weight within the same litter, the piglets with low birth weight numerically showed the greatest difference (CON vs. GH: 5.73 kg vs. 6.38 kg) in weight gain from birth to weaning compared to other levels (Table 2).The feed intake of creep feed showed high variation among litters.The piglets in GH displayed a consistently higher feed intake than those in CON over the 4-week lactation period, and a significant difference was observed in the second week (14.4 vs. 21.9g/piglet, p < 0.05).Throughout the entire lactation period, the average grass hay feed intake of piglets exceeded 57 g of grass hay in total.After weaning, no significant differences were observed between the two treatments in weight gain and feed intake (p > 0.05).

Gastrointestinal tract morphometry
The body weight of selected piglets for necropsy was not significantly different, despite a numerical difference of 9% between two groups (Table 3, p > 0.05).During the necropsy, it was observed that both the absolute and relative weight of the stomach (both full and empty), liver, and spleen were not affected by the presence of grass hay (p > 0.05).However, grass hay increased the weight of emptied small intestine and large intestine significantly (280 vs. 228 g, 88.8 vs. 79.3g, p = 0.008 and p = 0.033, respectively).Additionally, the large intestine was also found to be longer in GH than CON (p = 0.004).Nevertheless, there is no statistical difference in the relative weight of SI and LI to body weight (p > 0.05).

Microscopic morphometry of intestine
In the small intestine, the villus height and crypt depth in duodenum and ileum were not influenced by the treatments (Figure 1, p > 0.05).However, the average ratio between villus height and crypt depth of the ileum was higher in GH compared to CON (Table 4, p < 0.05).Furthermore, the villus height of jejunum was 40 µm longer in GH than CON (p < 0.05).In the large intestine, the crypt depth in CON was larger than in GH at weaning (p < 0.05).

Short-chain fatty acid profile in cecal and colonic content
In the cecum, grass hay increased the concentrations of acetic acid and propionic acid (Figure 2, 600 vs. 702 µmol/g dry matter for acetic acid, 185 vs. 255 µmol/g dry matter for propionic acid, p < 0.05).Additionally, the total SCFA concentration in the cecum of piglets in GH was also higher than CON, with values of 948 vs. 1,179 µmol/g dry matter (data not shown in Figure 2, p = 0.025).In contrast, in the mid-colon, the acetic acid concentration in GH was reduced (p < 0.05), and the total SCFA concentration in CON was numerically higher than GH (341 vs. 278 µmol/g dry matter).

Colonic microbiota community
Within the relative proportions of ASV, the Firmicutes and Bacteroidota phyla collectively accounted for more than 95% of entire community, with Fusobacteriota and Spirochaetota following closely in the phylum level (Figure 3A).At the genus level, microbiota composition exhibited a large variation, and we observed that Lactobacillus, CHKCI001, and Bacteroides were the most three dominant genera across all samples.Furthermore, there was a trend that the average Lactobacillus proportion in GH was higher than with CON, accounting to 19.6% vs. 8.0% (p = 0.080).The supplement of grass hay did not affect the alphadiversity at the ASV level, as indicated by the Shannon index comparison (Figure 3C, p > 0.05).However, the InvSimpson index tended to decrease in the GH group compared to CON (Figure 3B) (p = 0.051).The principal coordinates analysis (PCoA) on the microbial compositions of individuals showed no distinct clusters separating the two groups responsive to the treatment.

B C D E A
The villus height and crypt depth in intestine of piglets fed a common creep feed with or without grass hay during suckling phase.CON: control group, GH: grass hay group.Mean values within a row with different superscripts differ significantly (p < 0.05).
1 Body weight at autopsy as covariate. 2 Pooled standard error of the mean.

Discussion
This study investigated the impact of a separate provision of grass hay on supporting suckling piglets development.Our findings showed that the inclusion of a separate feeder containing chopped grass hay in the farrowing crate modified the metabolic profile and growth of the gastrointestinal tract.Fiber enrichment has been extensively tested in pig feeding due to its potential benefits, and selective inclusion of fiber was shown to promote GIT development and modify gut microbiome and immune status in finishing pigs and sows feeding (Jin et al., 1994;Mcglone and Fullwood, 2001;Claus et al., 2007;Heinritz et al., 2016).Unlike other feeding phases, appetite to solid feed of piglets in the suckling phase is markedly variable and unpredictable not only between litters but also within the same litter, even when providing what is considered a palatable formula (Pajor et al., 1991;Sulabo et al., 2010;Middelkoop et al., 2019a).
Therefore, the consistent increase in creep feed intake upon grass hay provision, is an important finding for practice.Other studies on inclusion of fiber in creep feed so far only showed numerical changes in creep feed intake (Hanczakowska et al., 2008;Van Hees et al., 2019), suggesting that the physical form of the grass hay in addition to its chemical traits may be important to affect voluntary solid feed intake in young piglets.Other types of insoluble fiber, such as wheat bran, have been observed to speed up the gastric mobility.This acceleration plays a crucial role in the gut-brain axis, regulating appetite signals to activate the feed intake and behaviors, which might contribute to the result in the present study (Benini et al., 1995;Inui et al., 2004;Li et al., 2023).Furthermore, it also might be interesting to investigate if the impact of feeding hay depends on the birth weight of piglets in a study involving more litters.The presence of extra feeders with diverse feed resources in the farrowing crate is capable of stimulating the suckling piglets' exploring and foraging behaviors (Middelkoop et al., 2019b).This, in turn, might lead to a reduction in the competition among piglets for teats access, as it is known that piglets with heavier birth weight are known to consume more milk per suckling event than their lighter littermates (Campbell and Dunkin, 1982).However, some other previous studies demonstrated that the supplementation with dietary fiber could suppress the appetite (De Leeuw et al., 2008;Jarrett and Ashworth, 2018).It may be attributed to the fact that fermentation of SCFAs in the colon can stimulate the secretion of gut-brain peptides and mediate the appetite signals via the vagus nerve (Bolognini et al., 2021).Whether this pathway is primarily activated via the fermentation in colon rather than other sections of large intestine needs to be fully elucidated, and non-pronounced change of colonic microbiome occurred in this study, playing a key role in regulating host's appetite, might also erase the decreased feed intake.Therefore, the effects seen in the GH group may go beyond the direct impact of fiber consumption.
The weight of visceral organs can reflect the piglet's maturation process (Elefson et al., 2021), but the grass hay provision induced no effect on stomach, liver, and spleen weight.Len et al. (2009) studied fiber levels and sources in weaned piglets and also found no effect on liver weight, yet a higher stomach weight after 33 days of feeding.Studies on the effect of fiber on spleen weight are limited, but in general, the development of spleen and liver remains closely related to the body weight growth from birth to weaning (Lanferdini et al., 2018;Elefson et al., 2021).It is worth noting that a sufficient duration of exposure to the diet might also play a role in its effect.For instance, pigs provided with high-fiber diet for an The short-chain fatty acid (SCFA) profile in cecal and colonic content of piglets fed a common creep feed with or without access to chopped grass hay during suckling phase.CON, control group; GH, grass hay group.extended period had heavier weights of liver and kidney weights compared to those on a lower fiber diet, but this effect was not as pronounced with a shorter feeding duration (Pond et al., 1988;Anugwa et al., 1989).Given the fairly short length of the suckling phase combined with the low intake in commercial farming, this aspect is hard to cope with.The response on stomach weight to insoluble fiber appears to be faster than other organs due to the bulking effect in weaned piglets (Rijnen et al., 2001;Len et al., 2009), although-as in our study-supplementing suckling piglets with cellulose and other fibers in creep feed did not alter the empty stomach weight (Van Hees et al., 2019;Choudhury et al., 2021).
Growing pigs fed high-fiber diets typically exhibit a larger gastrointestinal tract than those fed a common diet (Jørgensen et al., 1996;Freire et al., 2003;Pluske et al., 2003).We here demonstrated that this also occurs in suckling piglets when fed grass hay.This enlargement might be attributed to water-retentive and "bulking" properties of (insoluble) fiber (Tungland and Meyer, 2002;Dhingra et al., 2012), as observed in previous studies applying cellulose and oat hulls in creep feed (Van Hees et al., 2019).The absence of a significant difference in relative weight of small and large intestine might also suggest that the increased weight of intestine may not be sufficient to influence the overall carcass weight.Not only the macroscopic anatomy but also the microscopic architecture was affected, mainly shown by an increased ileal villus:crypt ratio, likely resulting from increased digestion of nutrients in the jejunum (Nigam et al., 2019).This observation may therefore reflect increased proximal nutrient digestion and absorption, rendering less challenge of undigested matter to the distal intestinal sections, hence explaining its higher villus:crypt ratio.Some of the changes in the villus:crypt ratio may originate from a response to intestinal injury induced by various agents, such as digestive pressure, promoting crypt hyperplasia (Da Cunha Ferreira et al., 1990;Potten, 1990;Pizarro et al., 2000).
A well-developed large intestine is essential for piglets absorbing fluid, electrolytes, and remaining nutrients, including SCFA, from the proximal sections efficiently.The physical barrier of the intestine also helps against microbial invasion (Williams et al., 2001;Xu and Cranwell, 2003).The 12% heavier and 10% longer large intestine due to grass hay thus is an interesting trait.Moreover, it coincides with increased production of SCFA, generating additional fuel for large intestinal enterocytes and cell proliferation (Scheppach, 1994).Further study is needed to identify the cause of the more proximal shift in fermentation site due to grass hay, but it is well-studied that cellulose-rich material on itself is poorly fermentable in pigs (Bachmann et al., 2021), especially young individuals.Hence, the likely explanation for the fermentation shift is an effect of the grass hay on the passage of the fermentable fractions of the ingested creep feed and milk oligosaccharides.Abimosleh et al. (2012) have reported that induced colitis was accompanied by deeper crypt depth in rats, and imbalanced cell proliferation is also a risk factor of neoplasia (Deschner and Maskens, 1982).However, one trait such as crypt depth within a complex population of intestinal cells may not represent the entire function of the LI (Snippert, 2016).While this perspective is insightful regarding the current study, further investigation is required for confirming the association between the structural The microbiota community analysis in the mid-colon of piglets fed a common creep feed with or without access to chopped grass hay during suckling phase.CON, control group; GH, grass hay group.characteristics of LI and its optimal functioning, such as cell proliferation, nutrient absorption, and other relevant factors.Despite marked changes in intestinal anatomy and morphology, consumption of grass hay did not exert major changes in colonic microbiota.Plenty of studies have reported the effect of fiber on the microbiota on weaning or fattening pigs (Kraler et al., 2016;Onarman Umu et al., 2018;Fouhse et al., 2019).Unlike older pigs, observing changes in microbiota composition in the suckling piglet intestine is challenging due to various factors such as creep feed patterns, the genital tract and feces of sow influence, genetic interactions, and pen environment, all of which contribute to the complex microbiota community (Kubasova et al., 2017;Chen et al., 2018).The trend of a lower alpha-diversity with GH in the colon may relate to the proximal shift of fermentation discussed higher.At the same time, the abrasive function of insoluble fiber may slough the epithelial mucus together with the microbes (Molist et al., 2009;Chen et al., 2013).The trend of increased abundance of Lactobacillus in the colonic microbiota when fed grass hay is in line with observations in rats and humans fed insoluble fiber (Gibson et al., 1995;Zhong et al., 2015), but in general, it is surprising that the microbiome was hardly affected by the grass hay intake.
Although there are still many lingering questions regarding long-term effects, feeding grass hay to suckling piglets shows promise and warrants further exploration in practical settings.Larger-scale studies will need to clarify if performance differences during suckling as well as throughout the entire fattening phase will appear.

Conclusion
Piglets readily consume grass hay in the pre-weaning period.Access to chopped grass hay during the suckling period in a separate feeder stimulated the feed intake of creep feed and promoted intestinal growth.Moreover, the consumption of grass hay exerted a more proximal shift in intestinal fermentation without prominent changes in microbial communities.
(A-D) The comparison of villus height and crypt depth between two treatments.(A) Duodenum, (B) jejunum, (C) ileum, (D) mid-colon.(E) The typical pictures of microscopic morphology in jejunum and mid-colon.Body weight at necropsy as covariate; VH, villus height; CD, crypt depth.Columns marked with * at the top indicate significant difference (p < 0.05).
(A) SCFA profile in cecal content.(B) SCFA profile in colonic content.Columns marked with * at the top indicate significant difference (p < 0.05).The error bars presented in the figure denote SEM.
(A) Relative abundance of the 5 most abundant phylum and 20 most abundant genera in the mid-colon.(B) Diversity metrics, alpha-diversity in Shannon index, and inverse Simpson (InvSimpson) index.(C) Principal coordinates analysis (PcoA) of Bray-Curtis beta-diversity.

TABLE 1
Composition (ingredients, nutrients)of creep feed (% as-fed basis) fed from day 2 to day 28 (day of weaning).

TABLE 2
Technical performance of piglets fed a common creep feed with or without access to chopped grass hay from birth to 9 weeks of age.
CON, control group; GH, grass hay group; same as below.a,b Mean values within a row with different superscripts differ significantly (p < 0.05). 1 Birth weight as covariate.2 Pooled standard error of the mean.

TABLE 3
The gastrointestinal tract morphometrics of piglets fed a common creep feed with or without access to chopped grass hay during the suckling phase.

TABLE 4
The villus height:crypt depth ratios in the respective small intestinal sections of piglets fed a common creep feed with or without access to chopped grass hay during the suckling phase.