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BRIEF RESEARCH REPORT article

Front. Cell. Infect. Microbiol.
Sec. Extra-intestinal Microbiome
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1355787
This article is part of the Research Topic

Acute Leukemias: Molecular Characterization, Leukemia-Initiating Cells, and Influence of the Microenvironment Vol. II

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No evidence on infectious DNA-based agents in pediatric acute lymphoblastic leukemia using whole metagenome shotgun sequencing Provisionally Accepted

Amadeus T. Heinz1, 2 Silke Grumaz3 Christoph Slavetinsky4 Michaela Döring1 Manon Queudeville5 Rupert Handgretinger6 Martin Ebinger1*
  • 1Department of Oncology/Haematology, University Children's Hospital Tübingen, Germany
  • 2Zentrum für Kinder-, Jugend- und Frauenmedizin, Klinik Stuttgart, Germany
  • 3Noscendo GmbH, Germany
  • 4University Children's Hospital Tübingen, Germany
  • 5Division for Pediatric Stem Cell Transplantation and Immunology, Clinic and Polyclinic for Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Germany
  • 6Abu Dhabi Stem Cells Center (ADSCC), United Arab Emirates

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The etiology of pediatric acute lymphatic leukemia (ALL) is still unclear. Whole-metagenome shotgun sequencing of bone marrow samples in patients with treatment-naïve ALL (n=6) was performed for untargeted investigation of bacterial and viral DNA. The control group consisted of healthy children (n=4) and children with non-oncologic diseases (n=2) undergoing bone marrow sampling. Peripheral blood of all participants was investigated at the same time. After bioinformatical elimination of potential contaminants by comparison with the employed controls, no significant amounts of microbial or viral DNA were identified.

Keywords: high-throughput sequencing, ALL, Shotgun sequencing, Infectious etiology, microbiome

Received: 14 Dec 2023; Accepted: 24 May 2024.

Copyright: © 2024 Heinz, Grumaz, Slavetinsky, Döring, Queudeville, Handgretinger and Ebinger. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Martin Ebinger, Department of Oncology/Haematology, University Children's Hospital Tübingen, Tübingen, 72076, Baden-Württemberg, Germany