Role of Lipid Accumulation and Inflammation in Atherosclerosis: Focus on Molecular and Cellular Mechanisms

Atherosclerosis is a chronic lipid-driven and maladaptive inflammatory disease of arterial intima. It is characterized by the dysfunction of lipid homeostasis and signaling pathways that control the inflammation. This article reviews the role of inflammation and lipid accumulation, especially low-density lipoprotein (LDL), in the pathogenesis of atherosclerosis, with more emphasis on cellular mechanisms. Furthermore, this review will briefly highlight the role of medicinal plants, long non-coding RNA (lncRNA), and microRNAs in the pathophysiology, treatment, and prevention of atherosclerosis. Lipid homeostasis at various levels, including receptor-mediated uptake, synthesis, storage, metabolism, efflux, and its impairments are important for the development of atherosclerosis. The major source of cholesterol and lipid accumulation in the arterial wall is proatherogenic modified low-density lipoprotein (mLDL). Modified lipoproteins, such as oxidized low-density lipoprotein (ox-LDL) and LDL binding with proteoglycans of the extracellular matrix in the intima of blood vessels, cause aggregation of lipoprotein particles, endothelial damage, leukocyte recruitment, foam cell formation, and inflammation. Inflammation is the key contributor to atherosclerosis and participates in all phases of atherosclerosis. Also, several studies have shown that microRNAs and lncRNAs have appeared as key regulators of several physiological and pathophysiological processes in atherosclerosis, including regulation of HDL biogenesis, cholesterol efflux, lipid metabolism, regulating of smooth muscle proliferation, and controlling of inflammation. Thus, both lipid homeostasis and the inflammatory immune response are closely linked, and their cellular and molecular pathways interact with each other.


INTRODUCTION
Atherosclerosis is an important cause of cardiovascular diseases such as ischemic heart disease and stroke. This complex multifactorial disease has chronic and progressive pathology characterized by lipid accumulation, low-grade inflammation in the walls of large-and medium-sized arteries, and endothelial dysfunction (1,2).
Two key factors in the pathogenesis of atherosclerosis are cholesterol deposition and chronic inflammation (3). Atherosclerosis has three significant stages including fatty streak formation, induction of atheroma, and atherosclerotic plaques (4).
Reactive oxygen species (ROS) and reactive nitrogen species (RNS) damage the cellular function of lipids, proteins, and carbohydrates, and cause lipid peroxidation and low-density lipoprotein (LDL) oxidation (5). Oxidized LDL (ox-LDL) can remain in the vascular intima. ox-LDL has a crucial role in the initiation and development of atherosclerosis through inducing endothelial cell (EC) dysfunction, increasing leukocyte adhesiveness, inducing the expression of leukocyte and monocyte adhesion molecules on the endothelial surface such as Vascular Cell Adhesion Molecule-1 (VCAM), Intercellular Adhesion Molecule-1 (ICAM), E selectin and P-selectins (3,6,7). Monocytes, T lymphocytes, and mast cells are taken up into vascular wall intima by these adhesion molecules. T cells can respond to inflammatory signals by producing γ-interferon (IFN-γ) and lymphotoxin, and tumor necrosis factor β (TNFβ) (8,9). Monocytes are converted into macrophages in the sub-endothelial space through Monocyte Chemotactic Protein-1 (MCP-1), Macrophage Colony-Stimulating Factor (M-CSF), and Interleukin-8 (IL-8). Macrophages uptake ox-LDL molecules via the scavenger receptor-A family to form lipid-laden foam cells. Yellow foam cells aggregate on the arterial walls and cause the development of fatty streaks (10,11). A fibrous atherosclerotic plaque cap is formed from the fatty streak during the migration of Smooth Muscle Cells (SMCs) from media to intima and SMCs proliferation (9,12). At advanced stages of atherosclerosis, Macrophages and T lymphocytes of fibrous atherosclerotic plaque cap secrete proteolytic enzymes such as metalloproteinase to reduce the stability of the fibrous cap and lyse the fibrous cap extracellular matrix. Breakdown of fibrous cap collagen content leads to coagulation process, blood clot formation, thrombus formation, and blockade of the arteries (3,13,14).
The pathophysiologic feature of atherosclerosis is an inflammatory, cellular, and metabolic process (4,8). So, elucidating atherosclerosis pathogenesis is vital for understanding disease progression and the development of new therapeutics. This review will discuss the role and significance of inflammation and lipid accumulation especially LDL in the pathogenesis of atherosclerosis with more emphasis on cellular mechanisms. Furthermore, this review will highlight briefly the role of medicinal plants, long non-coding RNA, and microRNAs in the pathophysiology, treatment, and prevention of atherosclerosis.

SOURCES OF THE INFORMATION
The comprehensive information in this review article was obtained from noteworthy scientific databases, including Web of Science, PubMed, Science Direct, Scopus, and Google Scholar. The main search terms used in this study were atherosclerosis, LDL, lncRNA, microRNA, cholesterol, inflammation, and medicinal plants.
Lipid homeostasis at various levels, including receptormediated uptake, synthesis, storage, metabolism, and efflux, as well as its impairment, are important for the development of atherosclerosis (22). Under normal physiological conditions, LDL in the cell can degrade in the lysosomes that prevent an imbalance of uptake, synthesis, efflux, and excessive lipid accumulation (23). The hypothesis of cholesterol retention in the arterial cells was suggested by Nikolai Anitschkow over 100 years ago (24). Enhanced plasma concentrations of cholesterol-rich apolipoprotein-B-containing lipoproteins are related to atherosclerosis. Lipoproteins can flux into and get out of the arterial wall via caveolin-1 and the scavenger receptor class B type I (SR-BI). Retention, or trapping, of cholesterol-rich apoB-containing lipoproteins within the arterial wall, is the key initiating event in atherogenesis. The retention of apoB-lipoproteins leads to lipid accumulation, triggers cellular responses within the artery wall, lesion development, maladaptive local responses, and plaque initiation (25,26). The response-to-retention occurs via interacting lipoproteins with proteoglycans of the arterial wall (26). The consequences of the retention of apoB-lipoproteins include accumulation of lipids and exposure to local enzymes within the vessel wall (27)(28)(29). Important enzymes involved in apoB-lipoprotein retention, aggregation, and atherogenesis are secretory sphingomyelinase (S-SMase), lipoprotein lipase, and phospholipase A2. The most action of these enzymes is accelerating further retention of atherogenic lipoproteins (26). Also, retention of cholesterolrich apoB-lipoproteins within the artery wall causes several modifications and significant biological consequences (26).
The major source of cholesterol and lipid accumulation in the arterial wall is proatherogenic mLDL (30). Desialylation, oxidation, formation of LDL self-associates, and LDL-containing immune complexes are the known LDL modifications (31). Also, all eight classes of scavenger receptors have been recognized as both native and modified LDL (32).
The first known atherogenic LDL modification is desialylation, and the trans-sialidase (neuraminidase) enzyme is responsible for LDL desialylation. The action mechanisms of intracellular lipid accumulation by desialylated LDL are performed in two ways; the first is binding, uptake, and degradation of LDL. The second is the evaluation of hydrolysis and esterification rates of lipids in LDL particles (33). LDL particles desialylation causes autoantibodies production, which forms circulating immune complexes (CIC) containing LDL (34). CIC causes the secretion of pro-inflammatory cytokines and macrophages apoptosis (34). Desialylated LDL causes the following consequences: lipid accumulation, enhancing its binding to the arterial proteoglycans, proliferative activity, connective tissue matrix components synthesis, breaking intercellular communication, chronic inflammation, and intracellular esterification of free cholesterol through preventing of the cholesterol acyltransferase esterifying activity in macrophages and cholesterol accumulation (24,34).
Oxidative lipoprotein modification by intimal oxidizing agents, proteases, and lipases leads to the generation of oxidized phospholipids (oxPLs), inducing leukocyte recruitment, leukocyte activation, LDL aggregation, formation of cholesterol crystals, and inflammation (16).
Modified lipoproteins, such as ox-LDL, and LDL binding with proteoglycans of the extracellular matrix in the intima of blood vessels, cause aggregation of lipoprotein particles, endothelial damage, leukocyte recruitment, and inflammation (26). Then, foam cell forms from aggregated modified apoBlipoproteins through taking up oxidized, proteolyzed, or lipolysed lipoproteins, taking up cholesterol crystals by macrophages and taking up lipoproteins by SMCs via different classes of scavenger receptors (SR), such as SR class A (SR-A1, also known as CD204), SR class B (CD36), and the lectin-like oxidized LDL 1 receptor (LOX-1, or SR-E1), which can identify and bind to modified lipoproteins (35)(36)(37). Also, aggregated mLDL may accumulate in macrophages, which is recognized by low-density lipoprotein receptor-related protein 1 (LRP1) and Toll-like receptor 4 (TLR4) or degraded via lysosomal synapses (38)(39)(40).
SR-A is expressed on the surface of macrophages by regulating various factors. Sac1 and Sac3 phosphatases maintain a constant level of SR-A expression in the endoplasmic reticulum (41). Upregulation of Sac1 expression increases the SR-A receptor abundance. Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-6 (IL-6) can upregulate the SR-A expression leading to LDL accumulation by macrophages (42).
CD36 with its ligands (mLDL, HDL, fatty acids, and VLDL) is involved in lipoprotein uptake and lipid metabolism (43). Signaling pathways, such as non-receptor tyrosine kinase (SRC), cJun NH2-terminal kinase (JNK), Rac (GTPase) protein, and nuclear factor-kB (NF-kB transcription factor), are activated by interacting mLDL and CD36, which can result in LDL absorption, oxidative processes, and the production of proinflammatory cytokines (32,44). LOX-1 from the E class SR family is involved in lipid accumulation, and its expression is increased in the intima under the inflammation and oxidative condition (32).
In the context of the response-to-retention, HDL has the following roles: interfering with the irreversible binding of plasma LDL to arterial wall proteoglycans, blocking SMaseinduced aggregation of LDL, omitting toxic lipids, ameliorating the maladaptive inflammatory infiltrate, inhibiting lipoprotein oxidation, EC protection and suppression of monocyte adhesion (45)(46)(47)(48)(49).

Role of Inflammation in Atherosclerosis
The role of inflammation in the pathogenesis of atherosclerosis was suggested in 1908 by Sir William Osler (50). Inflammation participates in all phases of atherosclerosis. For example, stable plaques and ruptured plaques are characterized by a chronic inflammatory infiltrate and "active" inflammation in the thinning of fibrous caps (21). Inflammation is linked with different risk factors of atherosclerosis (26,51). All risk factors of atherosclerosis cause inflammatory response (9). Cellular cholesterol and inflammation can affect the immune system and autoimmune diseases (26).
Ox-LDL and generally modified lipoproteins increase endothelial damage, leukocyte recruitment, and inflammation. Several studies revealed that high levels of E-selectin, ICAM-1, and VCAM-1 are expressed by inflamed endothelium (52). Thus, these activated endothelial cells are the local source of leukocytes recruited into an atherosclerotic lesion (21). One of the earliest signs of atherosclerosis is endothelial dysfunction or activation (53). High inflammatory responses lead to arterial wall thickening (53).
Various inflammatory cells such as macrophage foam cells and T lymphocytes participate in inflammatory responses and the progression of atherosclerosis (21). Different cytokines are produced by macrophage foam cells leading to activation of SMCs and extracellular matrix production (8).
In the initiation phase of atherosclerosis, monocytes and lymphocytes migrate into the inner arterial wall with the help of MCP-1 and T cell chemo-attractant. Inside the intima, monocytes are differentiated into macrophage foam cells under the influence of M-CSF. All activated cells release inflammatory cytokines and proinflammatory mediators. In the fatty-streak lesion, Tcells secrete TNF-β, IFN-γ, fibrogenic mediators, and growth factors that can cause the migration and proliferation of smooth muscle cells. Also, activated T-cells cause the following important inflammatory reactions: stimulating Matrix Metalloproteinase (MMP) production by macrophages in the lesion, producing IFN-γ, and halting collagen synthesis by the SMCs. It is considered that plaque formation is related to increased plasma concentration of C-Reactive Protein (CRP) (53). As mentioned above, inflammatory cells, macrophage foam cells and T-cells, and proinflammatory mediators (cytokines, interleukins) have important roles in all different stages of atherosclerosis.
Traditional risk factors of atherosclerosis are Hypercholesterimia, HDL, hypertension, obesity, and diabetes. The high level of LDL cholesterol in the blood causes artery and vascular smooth muscle cells (VSMCs) injury, induction of adhesion molecules and proinflammatory cytokines expression in macrophages and endothelial cells, and activation inflammatory response by expressing mononuclear leukocyte recruiting mechanisms (9).
The mechanisms of actions of HDL are inhibiting LDL oxidative modification, blocking the proinflammatory effects of ox-LDL, promoting antioxidant enzyme activity such as acetylhydrolase and paraoxonase, which neutralize oxidized lipids, and proinflammatory effects (9).
Angiotensin II (AII), as a powerful vasoconstrictor, is produced during hypertension (54). AII increases the growth of SMCs and facilitates smooth muscle lipoxygenase activity and results in speeding up inflammation. Also, it causes inflammation of endothelial intima via promoting the expression of cytokines (IL-6) and MCP-1 and superoxide anion production by the endothelium and SMCs of the artery (9,54). In fact, Ang-II has pro-atherogenic effects and increases endothelial oxidative stress. Also, it up-regulates the LOX-1 gene and causes the activation of apoptosis pathways and induction of endothelial dysfunction (54).
Oxidative stress, ROS formation, endothelial activation, and disruption of cellular defense systems in conditions of chronic hyperglycemia and diabetes promote inflammation. Glycated lipoproteins protect the proinflammatory action of cytokines in the arterial endothelium (55,56).
One of the most stable plasma biomarkers for low-grade systemic inflammation is CRP. It is a valuable tool for predicting, diagnosing, and prognosis of atherosclerosis. CRP has a direct role in promoting the inflammatory component of atherosclerosis. The significant mechanisms actions of CRP are: downregulating endothelial nitric oxide synthase (eNOS) to prevent nitric oxide releasing into the endothelium, destabilizing eNOS mRNA, increasing the release of endothelin (ET-1), adhesion molecules (VCAM-1, ICAM), MCP-1, migration of SMCs and facilitating LDL uptake by macrophage (59).
PAR has four types (PAR-1,−2,−3,−4). PAR-1 activation facilitates the binding of monocytes and leukocyte recruitment in the endothelium. PAR-1 and PAR-2 can enhance the leukocytes and platelets to the endothelium. It is proposed that the proinflammatory property of PARs is induced by IL-1 and TNF-α in inflamed cells (9).
CD40/CD40L is a proinflammatory system belonging to the TNF family. This protein is found in atherosclerotic plaques and expressed by activated macrophages, SMCs, vascular endothelial cells, and T lymphocytes. Binding of soluble CD40L (sCD40L), derived from activated platelet, to CD40 on SMC, and endothelium causes endothelial dysfunction, inflammation, and production of proinflammatory cytokines (IL-6, IL-1), VCAM-1, ICAM, MCP-1, MMPs, fibroblast growth factor, vascular endothelial growth factor, platelet activation, and thus the production of ROS and RNS (9, 60-62).
Interleukin-18 (IL-18) is made by monocytes and macrophages and its receptor is expressed on T lymphocytes (T helper). IL-18 binds to its receptors and causes inflammation and plaque formation via producing IL-1, TNF-α, and a positive feedback mechanism. The stability of plaque is reduced by increasing MMP expression (9). This proinflammatory cytokine causes induction of IFN-γ, inhibiting collagen synthesis, preventing thick fibrous cap formation, and facilitating plaque destabilization (63).
LOX-1 is found in endothelial cells, macrophages, and SMCs. It is bound only to ox-LDL and detected in all phases of atherosclerosis. The ox-LDL/LOX-1 complex causes the following impacts: increasing ROS production, death of SMCs, MMP influence on a fibrous cap, providing a route of entry for ox-LDL into endothelium, disrupting the normal endothelial function and monocyte adhesion, and infiltration. The stimulation of LOX-1 causes endothelial dysfunction, leukocyte adhesion, collagen degradation, and foam cell formation (64). Lp-PLA2 has performed its proinflammatory and proatherogenic functions by promoting monocyte chemotaxis, enhancing expression of mononuclear leukocyte adhesion molecules in endothelial cells, producing lysophosphatidylcholine (lysoPC) and non-esterified fatty acid moieties (9).

The Cellular Mechanisms of Inflammation and Lipid Accumulation in the Pathogenesis of Atherosclerosis
The intimal layer of the arterial wall is the location of the Atherosclerotic lesion. The endothelial cells separate the intima from the lumen of the vessel. Under the basal membrane, different types of cells such as macrophages, dendritic cells, foam cells, lymphocytes, and other inflammatory cells are found in intimal atherosclerotic lesions (65). Deeper layers include SMCs and pericytes that participate in immunity reactions. Pericytes secrete pro-inflammatory cytokines such as IL-1, IL-6, and TNF. They also act as phagocytes and antigen-presenting cells (66,67). Lesion development is associated with a local enhancement of the number of macrophages, loss of intercellular communication, and changes of Pericytes, macrophages, and SMCs phenotype (68).
Lipid droplets accumulate in the cytoplasm of Pericytes, macrophages, and SMCs and lead to change their appearance.
The presence of Pericytes, macrophages, and SMCs in the subendothelial space of the arterial wall is an early manifestation of atherosclerosis (68). Atherogenic mLDL is the primary source of lipids that are found in foam cells and the circulation of atherosclerotic patients. Atherogenic modification of LDL is mentioned in section 3.1. mLDL stimulates phagocytosis by pericytes and macrophages. Then inflammatory cytokines are secreted, which causes the attraction of immune cells to the location of the inflammation. Inflammatory cytokines cause the accumulation of intracellular lipids. Intracellular lipids accumulation leads to rupture of the cells (68). Also, increasing proliferative activity and stimulation of extracellular matrix synthesis are occurring in the phase of inflammatory reaction (69). Based on current consensus, endothelial activation and enhanced permeability is the key event in the atherosclerotic lesion development. Endothelial cells express cytokines and chemokines (IL-1, TNF-α, MCP-1, growth factors, and adhesion molecules). This leads to the interaction of circulating immune cells with endothelium and enhancement of the pro-inflammatory signaling at the emerging lesion site (68). As a result of the increase of pro-inflammatory cytokines, endoplasmic reticulum stress in the arterial wall cells and apoptosis have occurred (70). This process and cytokineinduced inflammation lead to interrupt the normal activity of mitochondria and then impaired mitophagy and apoptosis (71). mtDNA mutations have an important role in atherosclerosis. These mutations lead to impaired glucose and fat metabolism, increased oxidative stress, ROS generation, and cell death (68). ROS act as modulators of gene expression related to atherosclerosis development. The mutation spectrum of the mitochondrial genome is useful for the early detection of atherosclerosis (3,(71)(72)(73)(74).
Lysosome function is linked to inflammatory cytokine release and regulation of immune response. So, loss of lysosomal function in ox-LDL-loaded Macrophages is a general effect related to the excess lipid loading during atherosclerosis (75,76).

The Role of Long Non-coding RNAs in Lipid Accumulation and Inflammation
Long non-coding RNAs (lncRNAs) have been considered as a novel group of epigenetic regulators with significant roles in the pathogenesis and development of atherosclerosis. Also, these biomarkers have the potential for targeting them therapeutically (77). lncRNAs have multiple functions in a wide range of biological processes. They are involved in regulating macrophage, lipid metabolism and inflammatory, and immune responses (78,79).
Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has a protective effect against atherosclerosis lesions. The supportive effects of MALAT1 against the ox-LDL-induced apoptosis were performed through these action mechanisms: Upregulating endothelial-tomesenchymal transition (EndMT), competing with miR-22-3p, induction of autophagy by inhibiting the PI3K/AKT pathway or sponging miR-216a-5p, and suppressing the production of ox-LDL mediated pro-inflammatory cytokines such as IL-6 and IL-8 via sponging miR-155 (95)(96)(97)(98). The anti-inflammatory effect of MALAT1 is increasing lipid uptake in macrophages via interacting with nuclear enriched abundant transcript (NEAT1) (99,100). lncRNA myocardial infarction associated transcript (MIAT) has a protective effect against the ox-LDL-induced apoptosis through inhibiting miR-181b and signal transducer and activator of transcription 3 (STAT3) (101,102). From a therapeutic point of view, lncRNA-DYNLRB2-2 has antiatherosclerotic properties via different mechanisms, including promoting cholesterol efflux, inhibiting inflammation, increasing ABCA1 expression, inhibiting THP-1 macrophage foam cell formation, activating the LKB1/AMPK/mTOR signaling pathway, and inhibiting the lipopolysaccharide (LPS)-induced inflammatory cytokines such as TNF-a, IL-1b, and IL-6 in macrophages by reducing TLR2 expression (4, 103-105). From a therapeutic point of view, Anril causes cholesterol efflux and reduction of inflammatory cytokines such as IL-1b and TNF-α in ox-LDL-exposed THP-1 macrophages via inhibiting a disintegrin and metalloprotease (ADAM) expression (106). The most important lncRNAs involved in lipid accumulation and inflammation are summarized in Table 1 and Figure 1.

Role of microRNAs in Lipid Accumulation and Inflammation
MicroRNAs have appeared as key regulators of several physiological and pathophysiological processes in atherosclerosis including regulation of HDL biogenesis, cholesterol efflux, lipid metabolism, regulating of smooth muscle proliferation, and controlling of inflammation through the activation and infiltration of leukocytes via the vascular wall (108)(109)(110).
Moreover, numerous studies have demonstrated the beneficial role of miRNAs as therapeutic targets in the treatment of atherosclerosis and cardiovascular disease ( Table 2 and Figure 1) (131). The overexpression of miR-146a can inhibit the activation of the TLR4 signaling pathway and prevent ox-LDL accumulation (116). Overexpression of miR-146a significantly decreases intracellular LDL cholesterol content and secretion of IL-6 and IL-8, chemokine (C-C motif) ligand-2, and MMP-9 in macrophages by targeting CD40L (116,117). It was demonstrated that miR-146a regulates IL-1 receptor-associated kinase-1 (IRAK1) and TNF-receptor-associated factor-6 (TRAF6) (118). The protective role of miR-125a-5p against atherosclerosis was performed through regulating the proinflammatory response, lipid uptake by macrophages, decreasing content of the inflammatory cytokines: tumor growth factorbeta (TGF-α), TNF-α, IL-2, and IL-6 (115). The miR-125a-5p expression can cause the expression of LOX-1 and CD68 leading to a decrease of ox-LDL-stimulated macrophage uptake (115). Thus, it suppresses oxysterol binding protein like-9 (ORP9) and leads to reduction of lipid uptake and the secretion of inflammatory cytokines, including IL-2, IL-6, TNF-α, and TGF-β, in ox-LDL stimulated human primary monocytes (115). miR-223 is one of the most abundant miRs in LDL and HDL particles and inflammation (128). NOD-like receptor pyrin domain containing 3 (NLRP3) and ICAM-1 are the targets of miR-223. Upregulation of both these targets increases endothelial inflammation and causes leukocyte infiltration and inflammation connected with atherosclerosis (129,130). It inhibits cholesterol biosynthesis via suppressing the sterol enzymes 3-hydroxy-3methylglutaryl-CoA synthase 1 (HMGCS1) and methylsterol monoxygenase 1 (MSMO1) in humans (131). miR-10a upregulation can influence inflammation through decreasing IκB/NF-κB activation, downregulation of inflammatory biomarkers, such as MCP-1, VCAM-1, E-selectin, IL-6, and IL-8. So, it suppresses inflammatory molecules in endothelial cells   lipopolysaccharides and TNF-α (126,132). miR-302a can regulate ABC transporters, which are involved in cholesterol efflux. The action mechanisms of this miRNA are increasing ABCA1 activity, cholesterol efflux out of macrophages, and preventing foam cell formation and growth of the atheromatous plaque (77). miR-126 can affect the inflammatory state of vasculature by the activation and infiltration of leukocytes via the vascular wall. It inhibits VCAM-1. Thus, miR-126 inhibition leads to enhancement of the proinflammatory TNF-α expression, the activity of NF-κB (nuclear factor κB), the activity of VCAM-1, as well as leukocyte-endothelial cells interactions and atherosclerotic lesions formation (123)(124)(125). Overexpression of miR-155 can cause attenuated inflammation and the subsequent foam cell formation via miR-155/calcium-regulated heat-stable protein 1 (CARHSP1)/TNF-α signaling pathway and targeting of mitogen-activated protein kinase 10 (MAPK10) signaling pathway (111,112). It was suggested that miR-155 can contribute to the inflammatory processes through increasing STAT3 and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling and targeting the suppressor of cytokine signaling 1 (SOCS1) in ox-LDL-induced macrophages (113). miR-155 could decrease the lipid uptake in ox-LDL-stimulated cells (114). miR-33a/b is a key regulator of lipid metabolism. It plays a key role in regulating reverse cholesterol transport by inhibiting the expression of ABCA1 at the RNA and protein level and decreasing cellular cholesterol efflux to apolipoprotein A-I (ApoA-I) (119,120). Inhibition of miR-33a/b causes the following consequences: Increasing β-oxidation and decreasing fatty acid synthesis, ameliorating circulating lipids profile, and slowing down the progression of atherosclerosis (119,120). miR-92a expression in the endothelial cells of atherosclerosisprone areas is an important regulator of atherosclerosis development via targeting STAT3 and secreting IL-6 and MCP-1 (127). miR-34a can develop atherosclerosis via inhibiting cell cycle and SirT1 protein expression, inducing endothelial cell senescence and repressing cell proliferation (121).

Role of Medicinal Plants and Their Active Compounds in Atherosclerosis
In recent decades, medicinal plants and natural products have been considered as one of the most important antiatherosclerotic strategies for the treatment and prevention of atherosclerosis. Medicinal plants and natural products with their potential antioxidant, antiatherogenic, and anti-thrombotic properties, prevent atherosclerosis and its harmful complication through modulating the pathways of the inflammatory response, reducing cholesterolemia, free radicals, and decreasing vascular resistance (133,134). The most important medicinal plants with anti-atherosclerotic actions have been summarized in Table 3 and Figure 2.

CONCLUSIONS
Atherosclerosis is a chronic inflammatory disease that is continuous crosstalk between the lipid metabolism and immune-inflammatory pathways. Accumulation of intracellular lipids is a fundamental event in atherogenesis at the cellular level. The accumulation of intracellular modified lipids, especially ox-LDL, leads to foam cell formation. Inflammation participates in all phases of atherosclerosis. Lipid droplets accumulate in the cytoplasm of Pericytes, macrophages, and smooth muscle cells and lead to change their appearance. All risk factors of atherosclerosis cause an inflammatory response. Inflammatory cytokines cause the accumulation of intracellular lipids. Intracellular lipids accumulation leads to rupture of the cells. As a result of the increase of proinflammatory cytokines, endoplasmic reticulum stress in the arterial wall cells and apoptosis occur. This process and cytokineinduced inflammation lead to interrupt the normal activity of mitochondria and then impaired mitophagy and apoptosis. Thus, both lipoprotein metabolism and inflammatory immune response play vital roles in the initiation, perpetuation, and eventually, resolution of the atherosclerosis process.
This information leads to the development of new approaches to the diagnosis and treatment of atherosclerosis such as evaluation of microRNAs and lncRNAs. On the other hand, new developments based on using cell models, medicinal plants, and natural products as therapeutic tools have been developed to prevent lipid accumulation and foam cell formation. Recently, lncRNAs and miRNAs have been considered for the treatment and prevention of atherosclerosis. Hence, the therapeutic effects of these molecules, especially lncRNAs, have largely remained experimental at this time. Expressing the therapeutic effect of these molecules with more details and their mechanism of action needs more experiments and research in the future. Totally, various lncRNAs and miRNAs can prevent and treat atherosclerosis by regulating HDL biogenesis, cholesterol efflux, lipid metabolism, regulating smooth muscle proliferation, and controlling inflammation.
Medicinal plants and their active compounds can prevent atherosclerosis through the following mechanisms: in fact, medicinal plants decrease total cholesterol, LDL-C, and TG. Various bioactive compounds reduce LOX-1 expression. They inhibit ROS production. Natural products inhibit the foam cell formation and SR-B1 expression. Also, the production of IL-1β, TNF-α, INF-γ, MCP-1, and ICAM-1 is inhibited by different medicinal plants.

AUTHOR CONTRIBUTIONS
KM and MR-K: conceptualization. MR-K: methodology and supervision. KM, EB, and MR-K: writing-original draft preparation and writing-review and editing. All authors contributed to the article and approved the submitted version.