Evaluative Knowledge and Skills of Student Teachers Within the Adapted Degree Courses

Due to the COVID-19 pandemic, many university initial teacher education courses have been adapted into remote mode. Starting from specific topics of the training curriculum, the work focuses on adapting courses on evaluative knowledge and skills in an e-learning environment; it examined the development of student teachers’ evaluative knowledge (terminology and concepts) and skills (design of paper and pencil assessment tools) who took the adapted courses. A comparative study of two adapted university degree courses (University of Bergamo and Mediterranean University of Reggio Calabria in Italy – initial training of future primary school teachers) in the area of evaluation, was carried out. The study involved 155 primary school student teachers and made a mixed method investigation with sequential system. The first exploratory method collected quantitative data by an “ad hoc” questionnaire on student teacher’ knowledge, the second confirmatory method gained qualitative data through the document analysis of paper-and-pencil assessment tool on student teachers’ skill. The results highlight substantial similarities on knowledge (function of evaluation, difference between evaluation and assessment, object of school evaluation) and skill (ability to refer to real situations and promote situated knowledge) but also differences (construct of school “evaluation processes” and ability to provide different solutions for solving the task) e specific difficulty in implementing the “constructive alignment.”


INTRODUCTION
Assessment activities involve the entire education system, as assessment of students' learning and behavior in the classroom and as evaluations of school's teaching-learning interventions (Galliani, 2015;OECD, 2015). From a professionalization perspective (OECD, 2005;Darling-Hammond et al., 2017;Andrade and Heritage, 2018), teachers' evaluative competences are described as knowledge, skills, and attitudes useful for carrying out educational interventions and assessment actions in the context scholastic (Popham, 2004;Willis et al., 2013;Münster et al., 2017).
In the initial teacher education curricula, these evaluative competences are related to the subject to be taught, the didactic knowledge of the discipline, the assessment methods, the grading procedures as well as to the ethics of evaluation Ogan-Bekiroglu and Suzuk, 2014). The development of evaluative competences occurs through the connection between different training activities, i.e., lectures, seminars, exercises, etc. (DeLuca and Volante, 2016).
Due to the COVID-19 pandemic, many university initial teacher education courses implemented adequate solutions in order to ensure the development of students' competences, such as e-learning environments where to adapt learning activities no longer feasible face-to-face (IAU-UNESCO, 2020; Marinoni and van't Land, 2020;Gaebel et al., 2021). Starting from specific topics of the training curriculum, the work focuses on adapting courses on evaluative knowledge and skills in an e-learning environment; it examined the development of student teachers' evaluative knowledge (terminology and concepts) and skills (design of paper and pencil assessment tools) who took the adapted courses (Popham, 2004;Willis et al., 2013;Xu and Brown, 2016).
After the descriptions of two initial training curricula for primary school teachers at University of Bergamo and Reggio Calabria in Italy, methodologies and results of a comparative study conducted in the February-March 2021 are presented.

Perspective on Teacher Evaluation Competences
The scholars proposed several definitions about teachers' evaluative competencies over the years: teachers' skills "as evaluators" (Roeder, 1972), evaluation procedures and classroom assessment competences (Stiggins and Faires-Conklin, 1988), assessment literacy (Popham, 2004(Popham, , 1995Xu and Brown, 2016), evaluation competencies and assessment capability (DeLuca et al., , 2018. Moreover, various areas of competence have been identified: preparation in using the tests (Roeder, 1972); collection of data useful for the decision-making (Stiggins and Faires-Conklin, 1988); create evaluation tools and use those made by others (Popham, 1995); reporting achievement, synthesize assessments, item reliability, validity, etc. . The definition of teachers' evaluative competence takes on different nuances according to the functions attributed to the action of evaluating (Stiggins and Faires-Conklin, 1988): classroom decision making; assessment as interpersonal activity; providing a clear and stable target; tools to assess achievement and other traits; providing feedback; meaning of quality assessment; focus on assessment policy. A competent teacher in evaluation is assumed therefore capable not only to diagnose areas of strength and weaknesses of students and groups, to know how to define teaching interventions based on educational needs, to know how to communicate educational objectives and social expectations, but also to know how to evaluate the effectiveness of teaching interventions (OECD, 2013).
From the perspective of teacher professionalization (Willis et al., 2013), the evaluation competencies of teachers are defined as closely related to concepts, representations, and personal beliefs on evaluation (Münster et al., 2017) -as assessment and grading methods, feedback, self-assessment, and peer evaluation as well as the ethics of evaluation -but also to disciplinary knowledge and pedagogical content knowledge (Shulman, 1987;Xu and Brown, 2016). Xu and Brown (2016, pp. 151-153) identified some subtheme synthetizing research on teacher training in evaluation (as "assessment literacy"): assessment courses, assessment training programs and resources, the relationship among assessment training, teacher conceptions of assessment, teacher assessment training needs, and self-reported efficacy. Despite being considered a problematic area of study (La Marca, 2006;DeLuca et al., 2018), the training of teachers in evaluation is a subject of growing interest for decision makers (Stiggins and Faires-Conklin, 1988); the logic of accountability leads to asking for validated information on students' learning level and teacher preparation (OECD, 2005;Darling-Hammond and Adamson, 2010;Darling-Hammond et al., 2017). DeLuca and Johnson (2017, p. 125) are confident that "across pre-service and in-service contexts of assessment education there are consistent results that effectively support teachers' assessment capability" and that learning to assess involves the ability to select priorities with respect to the context of work, the alignment with professional development criteria.

Initial Teacher Training Curricula and Evaluation Competences
The research has provided a number of key elements for identifying teachers' evaluation competences (Brookhart, 2011), useful for focusing peculiar topics for professional development programs in evaluation and within pre-service training curricula (see Table 1; O'Leary, 2008, pp. 111-112).
Although training of teachers on evaluation is recognized a priority, given the link with the improvement of schools and students' learning, the investigations on it were carried out mainly from general perspectives, without connection with daily practice or standards .
Studies on initial training in evaluation focused attentions on curricula and methods of providing (contents, teaching strategies, etc.). Some of these focused on how specific evaluation skills are exercised at higher education and university level (relationship with other disciplines; use of specific educational activitieslectures, seminars, laboratories -and assessment and assessment tools -DeLuca et al., 2018). These allowed to clarify the very construct of "assessment literacy" Ogan-Bekiroglu and Suzuk, 2014;Pastore, 2020).

Effectiveness of Training Courses
Studies on effectiveness of initial training courses in evaluation taken different perspectives. Studies inspired by the "processoriented" model (Grossman and Schoenfeld, 2005) focused on the evaluation of "profound knowledge of the subject, " based on the assumption that those who know, can teach and also know how to evaluate. Shepard et al. (2005), from an opposite perspective, considered evaluation skills as a specific declination of basic ones -as planning and instruction. DeLuca's investigations DeLuca and Bellara, 2013), however, found such studies lacking objective evidence on effectiveness of training courses and indicate this lack

Topics Focus
Assessment terminology and concepts The terminology, concepts and key principles teachers, inspectors, in-service providers and teacher educator should understand The role of assessment in learning and teaching The implications of the phrase assessment is integral to teaching and learning for classroom practice. Distinguishing between assessment of learning and assessment for learning Sizing up assessment The nature and long lasting effects of assessments teachers carry out in the first 2 weeks of the school years Planning assessment The importance of identifying key learning outcomes across the different subject areas and matching them to assessment methods/ tools Assessment during the teaching and learning process The nature, potential and conduct of formative assessment including provision for appropriate feedback. Assessment approaches that can be integrated with ongoing teaching and learning to improve achievement Observation as an assessment tool The nature of informal observation in classrooms. Planning for and conducting systematic observations Paper-and-pencil assessments of achievement The role of teacher made tests and external assessments of achievement in teaching and learning. Planning, constructing and administering tests; using test data Performance assessments Using hands-on tasks to assess and improve learning processes The assessment of pupil disposition Approaches to assessing students attitudes Using assessment to motivate pupils and to develop positive attitudes and interests Assessing cross-curricular skills.
Approaches to assessing problem-solving, critical thinking, and reasoning proficiency Ethical and legal issues Misuses of assessment. Equity issues, Implications of government legislation for assessment in schools as a contributing factor to the low assessment capability of beginning teachers. The "effectiveness" concept refers to the property of something to produce the desired results. The effectiveness of a training intervention is linked to the improvement of knowledge, skills, and performance of the people involved, from an absolute point of view and in terms of transfer, i.e., the ability to apply the knowledge received and the skills developed in real life contexts and work (Basarab and Root, 1992). Such improvement can be estimated through several procedures: comparison of learning before-after training; during the training; immediately after training; as a follow-up to the intervention. As a descriptive model of the effectiveness of a training course, the updated model of Kirkpatrick (1979) and Lipowsky and Rzejak (2015) has been proposed. It identifies four levels: a. reaction -as satisfaction with the training received; b. learnings -as information or procedures learned, but also changes in attitudes, beliefs and levels of motivation; c. behaviors -as application of new knowledge in teaching practice; d. results -impact on the organization of the school (as increase in student learning; increase in motivation, etc.).

Effectiveness of Online Courses and Adaptation
In times the pandemic due to COVID-19 made it necessary to adapt the degree courses (IAU-UNESCO, 2020; Marinoni and van't Land, 2020;Gaebel et al., 2021). Theoretical and operational studies were carried out on the adaptation of degree courses (Rapanta et al., 2020;Agrati and Vinci, 2021).
Systematic studies on how teachers' evaluation competences have been taught online -in a "hybrid" mode, which guarantees face-to-face or remote activities (Allen et al., 2016;Perla, 2021) or in e-learning environments, through resources specifications (Vai and Sosulski, 2015;Poulin and Straut, 2016;Read et al., 2019), and e-tivity (Salmon, 2002) -are very few. In order to investigate the development of the evaluative competencies (knowledge and skills) of student teachers involved in adapted university courses, it is possible to use the Hamtini model (Hamtini, 2008). As adapted version of the Kirkpatrik's model, it replaces the four (reaction, learning, behavior, and results) with three (interaction, learning, and results) levels (Hamtini, 2008).

HYPOTHESES
The broader aim of this study is to get information on initial teacher training in evaluation, provided at the university, based on a descriptive investigation. Assuming specific topics of the training curriculum ("assessment terminology and concepts" and "paper-and-pencil assessments of achievement" [11 -see Table 1] as objects of analysis, the study aimed at known  • what knowledge (evaluation terminology and concepts) have student teachers gained? • have student teachers been able to design assessment tools?

Context, Sample, and Method of Investigation
A comparative study of two university courses (University of Bergamo, Italy; Mediterranean University of Reggio Calabria)degree courses aimed at the initial training of future primary school teachers -in the area of evaluation, was carried out ( Table 2). The two university courses lasted 64 h and alternated online training activities: theoretical lectures (the basics of evaluation, evaluation terminology, and concepts, etc.), thematic in-depth seminars (evaluation and assessment tools, skills assessment, etc.), and group laboratory activities (analysis of standardized tests, development of paper-and-pencil tools, etc.).
The study involved 155 primary school student teachers aged 27 on average, mostly women; it was made in the February-March 2021 period.
The comparative table below (Table 3) shows the online resources used to convert specific instructional activities (webinars, pdf files, forums, etc.). The adaptation of the two courses analyzed made use of the same articulation of online resources.
A mixed method design investigation with sequential system was made (Tashakorri and Teddlie, 2003;Cameron, 2015). The first exploratory method (quantitative data) produced early inferences useful to the second confirmatory method (qualitative data). The last synthetic meta inference was carried out through triangulation (Figure 1). Based on the Kirkpatrick model, adapted to the e-learning environment (Hamtini, 2008) attention was focused on student teachers' learnings, as declarativeprocedural knowledge (knowledge -1) and skills (skills -2).

Procedure and Tools
The first exploratory method collected quantitative data by an "ad hoc" questionnaire on evaluative student teacher' knowledge, the second confirmatory method gained qualitative data through the document analysis of paper-and-pencil assessment tool (in this case, the "authentic task" tool), as regards the evaluative student teachers' skills. All of student teachers involved (n. 155 = UniBg n. 81; UniRc n. 74) have produced the paper-andpencil assessment tool; n. 130 of them replied to the questionnaire on knowledge (UniBg n. 62; UniRc n. 68).
The "ad hoc" questionnaire was administered online through the University platform at the end of the courses. The questionnaire was structured in four close-ended items ( Table 4): it focused on specific terminology and concepts -evaluation function (question 1: value, judging, classifying), difference between "evaluation" and "assessing" (question 2: specific case, different plan), school evaluation objects (question 3: verifying students' knowledge and skills, evaluate competences, assessing learnings), learning outcomes and processes (question 4: assessing student outcomes, assessing teaching processes).
The descriptive statistical analysis on the quantitative data collected by the knowledge questionnaire proceeded to calculate via spreadsheet the mean and standard deviation of the responses ( Table 5).
The student teachers' ability (2) was inferred from the document analysis on paper-and-pencil assessment tools developed and uploaded by student teachers to the platform within thematic workshops at the end of the course. The paper-based assessment tool produced by the students was the "authentic assignment" (Comoglio, 2002;Herrington and Herrington, 2007;Herrington et al., 2014;Tessaro, 2014;Castoldi, 2018;Grion et al., 2019;Perla and Vinci, 2021), that is a "significant tasks, which agree to the (school) student of this experience and make the discovery of knowledge, to relate to it with a curious spirit, sharing experience with others; to acquire, in this way, significant knowledge, that is, recognized as 'important' or necessary by the subject, in order to navigate within a problematic condition verification" (Grion et al., 2019, p. 94). The format of "authentic task" provided to teacher students reported elements as educational goals, focus competence (linked to national curriculum), learning objective(s; linked to specific discipline), age of pupils, operational task, expected product, implementations (times, spaces, work phases and activities, resources, methods and tools), evaluating rubric, self-assessment procedure, methods of peer evaluation.
For the documentary analysis of the "authentic tasks" -and, consequently, the inferring of the ability in the "paper-and-pencil assessment tools" design -"criteria of relevance" (De Ketele and Gerard, 2005) have been identified, due to the complex and "open" nature of the tool, namely: • (real situation) reference to a real or likely situation/context, capable of promoting situated knowledge (and not decontextualized, as usually happens in knowledge assessment tests); • (different solution) possibility of different solutions for solving the task; • (challenging) complex, dynamic, and combinatorial nature of the knowledge required for the solution of the task, such as to assume a "challenging" connotation for the school student who has to solve it; • (transposition) transposition of the problem from the specialized language of a discipline to natural language; • (consistency) consistency between the expected product and the key competence selected for the structuring of the task.

Measurement
The analysis of the answers to the student teacher's questionnaire was carried out by calculating the mean for each alternative answer and the related standard deviation. Table 5 shows the data as a percentage. The documentary analysis of the "authentic tasks" obtained quantitative data. In fact, the average scores and related standard deviations were calculated with reference to the five "relevance criteria" identified. Figures 2, 3 show the quantitative data -, respectively, of UniBg and UniRc -of the document analysis of the "authentic tasks." The average scores obtained on the basis of the five "relevance criteria" are reported.
The documentary analysis was conducted by assigning a score to each criterion and proceeding to the sum of the scores obtained ( Table 6).
The triangulation process proceeded (Salkind, 2010) by crossanalysis between answers' measurement and the average score of the "authentic tasks" ( Table 6). Table 5 shows a general homogeneity and a specific difference between Bergamo and Reggio Calabria, as regards the student teachers' knowledge (1). General homogeneity is found with respect to the questions nn. 1, 2, and 3. Student teachers from Bergamo and Mediterranea: -assign to valuation the function of expressing a value for the purposes of a judgment (UniBg = 37.1%; UniRc = 42.6%). However, it should be noted the high dispersion for both data (UniBg = 1.03; UniRc = 1.09); -consider the assessment a specific case of evaluation (UniBg = 48.4%; UniRc = 80.9%). Although a reduced dispersion is noted for both data, the responses of UniRc are more numerous than UniBg (+32.5%); -identify the school evaluation object to verify both the learning and the skills of the students (UniBg = 43.5%; UniRc = 82.4%). Also in this case the responses of UniRc are more numerous than UniBg (+38.9%).

Evaluation Terminology and Concepts
As regards the knowledge related to the evaluation processes carried out at school (question 4), a difference emerges between the student teachers. UniBg student teachers believe that school evaluation is about both the learning processes of the students and the teaching processes of the teachers (46.8%). Instead, UniRc student teachers mainly consider students' learning outcomes and learnings processes (45.6%). It should be noted, however, that the gap between the answers to question no. 4 is not as broad as in the previous answers.
Research question n. 1 -what knowledge (evaluation terminology and concepts) have student teachers gained? The italic values are the highest per type of response.
Almost half of student teachers attribute to evaluation, in general, the function of judging events, things, objects, etc. The absolute majority of them consider the assessment a specific case of evaluation. The absolute majority of student teachers always consider the students' knowledge, skills and competences as objects of school evaluation. There is disparity, however, in the evaluation processes that take place at school: some consider only the learning processes of students, other also the teaching processes of teachers.
It would be indicated to deepen the investigation to know if this difference in knowledge depends on the contents delivered in the university course -beyond the similar didactic organization that was followed for the adaptation of online provision (Table 3) -or on a sort of "local culture" in evaluation topics.

Design of Paper-and-Pencil Tool
The data relating the document analysis of paper-and-pencil assessment tool ("authentic task") are shown in Table 6. The documentary analysis, with reference to the above-mentioned relevance criteria, shows some evidences.
Research question n. 2 -have student teachers been able to design assessment tools?
Almost all students (Tot. 91%; UniBg 91.4%; UniRc 90.5%) designed the authentic task referring to a real situation capable of promoting situated knowledge: there are no particular differences FIGURE 2 | Document analysis of "authentic task" (UniBg). between the two groups. This could indicate a widespread awareness on the part of students to refer to real or likely contexts in designing the authentic task.
With regard to the criteria "different solution, " "challenging, " and "transposition" some differences between the two groups emerge. As regards the possibility of different solutions for solving the task (different solution), the UniRc student teachers show higher response rates (75.7%) than UniBg (56.8); the same is true for the criterion related to complex, dynamic, and combinatorial nature of the knowledge required for the solution of the task, such as to assume a "challenging" connotation for the school student who has to solve it (challenging; UniRc: 81.1%; UniBg 69.1%). As regards the criterion relating to the transposition of the problem from the formalized (specialized) language of a discipline to natural language (transposition), on the other hand, responses are higher in the UniBg group (81.5%) than in UniRc (71.6%). It should be noted, however, that the gap between the answers is not broad.
A data that shows wide homogeneity between Bergamo (44.4%) and Reggio Calabria (48.6%) concerns the criterion of "consistency" between the expected product and the key competence selected for the structuring of the task: the figure in both groups is significantly lower than for the other criteria. This suggests the difficulty of designing an authentic task taking into account the coherence between the key competence, to be promoted, and the final assessment product. It also highlights a difficulty of some student teachers to implement "constructive alignment" (Biggs, 2014, pp. 5-6). The constructive alignment occurs when the learning activities that we ask students to engage in help them to develop the knowledge, skills and understandings intended for the unit and measured by our assessment (Tyler, 1949). The term "alignment" refers to the fact that the teacher provides a learning environment that supports the appropriate learning activities to achieve the intended learning outcomes: teaching methods and assessment tests must be aligned with the learning activities presupposed by the intended outcomes; the "constructive" aspect refers to the fact that students construct meanings through relevant learning activities and, knowing what the intended learning outcomes are and at what level, they are more likely to feel motivated and interested in the contents and activities planned by the teacher to facilitate their learning. Involving students also involves making them reflect on their learning process and their perceptions and opinions regarding the constructive alignment process (Serbati and Zaggia, 2012, p. 18).

LIMITATION AND PROSPECT OF THE STUDY
With regard to the analysis/validation of "authentic tasks" tools, given the complexity and "openness' of them, " specific relevance criteria have been identified. In the validation of an evaluative task based on competences, it was not possible to rely on criteria of validity and reliability (De Ketele and Roegiers, 1993;De Ketele and Gerard, 2005), designed for the validation of tests and/or description of a reference population (De Ketele and Dufays, 2003), but it was deemed appropriate to identify other criteria of documentary analysis closely linked to the complex and situational nature of the authentic task: in fact, the phenomenon under study is the result of a strong systematic effect and many individual or situational random factors.
At methodological level, the validation of tests for competences, such as authentic tasks, is still a challenge. There is not enough scientific evidence to support the identification of effective documentary analysis criteria: on the one hand, capable of meeting quality standards and scientific rigor; on the other hand, sensitive to the subjective, qualitative, and difficult to standardize descriptive dimensions of a complex phenomenon such as competence.
Some limitations of the work are not lacking. The first critical issue is linked to the terminology under study, since in the Italian lexicon the term "assessment" is used indifferently for two distinct processes, such as "assessment" and "evaluation, " whose meanings and representations by the students would deserve further investigation (Thomas, 1995). In addition to the need to disambiguate the terminology in use, there is also the variability of the contextual conditions that can certainly influence the results: the courses under examination, although homologous in terms of content, belong to two different geographical and cultural contexts.
The second critical point concerns the disciplinary context in which the work was carried out, i.e., the involvement of students of pedagogical courses on the subject of evaluation, with the possibility of reciprocal conditioning between the content of the teaching and the field of investigation of the research: future research with an experimental design and addressed also to non-educational courses could be useful to understand if some results obtained -and especially the training of evaluative skills, nowadays considered "soft skills" and defined as "just-intime" (EC, 2020), i.e., immediately applicable in multiple work contexts -can be considered valid regardless of the specific disciplinary context in which the work was carried out.

CONCLUSION FOR EDUCATIONAL PRACTICE
The results show that the average levels of evaluative knowledge and skills of teaching students (namely, the design of paper-andpencil tools) -both from Bergamo and Reggio Calabria -are more than satisfactory. With reference to the first question of the research (terminological knowledge and evaluation concepts), the students involved grasp the difference between "assessment" and "evaluation" and a broad idea of school evaluation processes -addressed as much to knowledge, as to the skills and competences of pupils. Regarding the second research question (ability to design evaluation tools) a good level of performance and products has been detected.
The design of the authentic task, which is functional to make the application of the evaluative criteria understood, had the role of promoting evaluative literacy, an essential competence in the future personal and professional life of young people (Sambell, 2011;Boud and Soler, 2016), yet often neglected in school and university practices. Socio-constructive contexts in which to activate teaching-learning processes of an interactive type, based on cooperation  should be implemented. Furthermore, assessment models based on real-life performances and competences, useful to promote the understanding of quality standards and the comparison of assessment criteria (Tai et al., 2016(Tai et al., , 2017 should be shared. The experience conducted responds to the need to train students, future teachers, to take an active role and reflect on their own learning in a more engaging way through one of the most important transversal skills for future personal and professional life: the ability to develop evaluative judgments, i.e., to create, use, and apply evaluative criteria to discriminate objects or make decisions about external situations or themselves (Restiglian and Grion, 2019, p. 197). This overcomes Assessment of Learning approaches, which see the student taking a passive role in the assessment process (Boud and Falchikov, 2007), to promote a perspective that makes students participate from the design of the assessment process and autonomous in their ability to evaluate their own learning (Boud et al., 2018): such learning itineraries fall fully within the approaches of assessment for learning (Sambell et al., 2013;Grion and Serbati, 2017) and sustainable assessment (Boud and Soler, 2016).
Research has also shown the importance of adapting a university course in remote mode through careful planning of the learning environment and the targeted use of online technologies and resources, to support evaluative design practices in the university: this suggests the need to train university teachers in the development of "sophisticated" skills  that make them able to choose, use, transform disciplinary content into "digitized disciplinary content" and to know how to effectively design learning environments starting from the potentialities inherent in new technologies for teaching, able to bridge the traditional gap between the world of education and real contexts.
Investigation has highlighted the role of documentation in the formation of students' planning, evaluative, and reflective skills (Vinci, 2021), showing how the careful structuring of a documentary device has a structuring and transformative function at a cognitive and metacognitive level and assumes the function of scaffolding for more effective and lasting learning. The reflection on the documentary format has also had the result of making the students -future teachers of pre-school and primary schools -aware of the essential link between planning, documentation, and school evaluation. The structuring of an ad hoc documental device for the design of the authentic task has allowed students to collect information from which to design the authentic task, acting as a mediating element (Damiano, 2013) in the performance required between the theoretical knowledge already acquired (through the introductory lessons of school assessment) and the operational skills required in the structuring of the task, then between knowledge acquired in the university course and skills required in the professional field.
Fostering student teachers' ability to analyze, design, and evaluate implies the renewal of teachers' professional competences and in particular of the documentary competence (Perla, 2012(Perla, , 2017(Perla, , 2019: hence the need to design specific methodological devices that can support teachers in their evaluation and self-assessment actions, as well as to advance research on models for teachers' professional development.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

ETHICS STATEMENT
Ethical review and approval was not required for the study on human participants in accordance with the local legislation and institutional requirements. Written informed consent for participation was not required for this study in accordance with the national legislation and the institutional requirements.