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Front. Endocrinol. | doi: 10.3389/fendo.2019.00272

Protective effect of resveratrol on benzo(a)pyrene induced dysfunctions of steroidogenesis and steroidogenic acute regulatory gene expression in Leydig cells

Bhaswati Banerjee1, Supriya Chakraborty1,  Pratip Chakraborty2, Debidas Ghosh3 and  Kuladip Jana1*
  • 1Bose Institute, India
  • 2Institute of Reproductive Medine, India
  • 3Vidyasagar University, India

Benzo(a)pyrene [B(a)P] is the toxic environmental Polycyclic Aromatic Hydrocarbon (PAH), that exerts male reproductive dysfunctions. In this study the molecular mechanism of B(a)P induced Leydig cell steroidogenic dysfunctions and its protective mechanism of action with a natural Aryl hydrocarbon receptor (AhR) antagonist and anti-oxidant, Resveratrol (Res) has been investigated. B(a)P exposure induced ROS mediated steroidogenic imbalance via activation of p38MAPK and repression of testosterone level as well as other steroidogenic enzymes like CYPIIA1, 3β-HSD, 17β-HSD expressions. B(a)P exposure also decreased StAR protein expression along with increased of DAX-1 protein, a transcriptional repressor of StAR gene expression as well as decreased SF-1 protein also, a transcriptional inducer of StAR gene expression. The study has established that Resveratrol as a potential agent combating the deleterious effect of B(a)P on Leydig cell steroidogenesis. Resveratrol treatment resulted significant protection against B(a)P by scavenging ROS and modulating the transcriptional regulation of anti-oxidant enzymes. Furthermore, Resveratrol also prevented stress kinase like p38 MAPK activation and increased StAR protein expression through the reduction of DAX-1 expression. Moreover, the testosterone production was efficiently restored with Resveratrol treatment. ChIP assay also revealed that resveratrol improved SF-1expression which further increased the StAR gene expression. Resveratrol acted efficiently against B(a)P, through its anti-oxidative properties as well as inhibits p38MAPK and increased steroidogenesis and StAR gene expression through the modulation of SF-1 gene expression.

Keywords: B(a)P, Leydig cell, , ROS,, StAR,, p38 MAPK, SF1

Received: 04 Jan 2019; Accepted: 12 Apr 2019.

Edited by:

Ranjith Ramasamy, University of Miami, United States

Reviewed by:

Himanshu Arora, University of Miami, United States
Carolina Jorgez, Baylor College of Medicine, United States  

Copyright: © 2019 Banerjee, Chakraborty, Chakraborty, Ghosh and Jana. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Kuladip Jana, Bose Institute, Kolkata, India,