Resistance Isn't Futile: The Physiological Basis of the Health Effects of Resistance Exercise in Individuals With Type 1 Diabetes

The importance of regular exercise for glucose management in individuals with type 1 diabetes is magnified by its acknowledgment as a key adjunct to insulin therapy by several governmental, charitable, and healthcare organisations. However, although actively encouraged, exercise participation rates remain low, with glycaemic disturbances and poor cardiorespiratory fitness cited as barriers to long-term involvement. These fears are perhaps exacerbated by uncertainty in how different forms of exercise can considerably alter several acute and chronic physiological outcomes in those with type 1 diabetes. Thus, understanding the bodily responses to specific forms of exercise is important for the provision of practical guidelines that aim to overcome these exercise barriers. Currently, the majority of existing exercise research in type 1 diabetes has focused on moderate intensity continuous protocols with less work exploring predominately non-oxidative exercise modalities like resistance exercise. This is surprising, considering the known neuro-muscular, osteopathic, metabolic, and vascular benefits associated with resistance exercise in the wider population. Considering that individuals with type 1 diabetes have an elevated susceptibility for complications within these physiological systems, the wider health benefits associated with resistance exercise may help alleviate the prevalence and/or magnitude of pathological manifestation in this population group. This review outlines the health benefits of resistance exercise with reference to evidence in aiding some of the common complications associated with individuals with type 1 diabetes.


INTRODUCTION
Notwithstanding their etiological, pathophysiological, and epidemiological complexities, the severity and incidence of complications associated with type 1 diabetes (T1D) are lower in physically active individuals (1,2). Conversely, at a population level, sedentariness constitutes the fourth leading risk factor for mortality (3), such that individuals with the highest rates of sedentariness are up three times more likely to suffer premature mortality than their physically active counterparts (4). Therefore, whilst pharmaceutical and technological therapies aid glycaemic management, the utility of simple, holistic approaches such as regular physical activity (PA) constitute attractive adjunct treatment options, that alongside standardised medical care facilitate the improvement of T1D related pathological complications. However, a research and advocatory emphasis has been placed on the implementation of sustainable moderate intensity continuous exercise (MICE) in those with T1D, with less discernible evidence exploring alternative exercise modalities such as resistance exercise (RE). Whilst the health benefits associated with MICE are by no means immaterial, longstanding T1D is often accompanied by complications that impact the ability of participants to comfortably perform their rhythmic and/or pounding nature. Furthermore, MICE often fails to maximise the development of skeletal muscle mass and strength, both of which are features that facilitate improvements in various metabolic, neuroendocrine, and osteopathic processes.
As such, the utility of incorporating RE to assist the functional development of skeletal muscle integrity constitutes an important feature of the current exercise guidelines for those with T1D (5).

METHODS
The authors undertook a detailed PubMed literature search for the following key words: "resistance exercise, " "exercise, " "physical activity, " "strength training, " "weight training, " "type 1 diabetes, " "T1DM." The reference lists of systematic reviews, reviews, and included as well as excluded articles were manually screened for studies considered to be relevant. The literature search was conducted by the corresponding and secondary lead authors with additional suggestions and/or missed literature being provided by co-authors when necessary.

IMPORTANCE OF PHYSICAL ACTIVITY AND EXERCISE IN PEOPLE WITH TYPE 1 DIABETES
Research has consistently demonstrated the inverse relationship between PA and a reduced risk of diabetes-related complications (6,7), including both mental well-being (8), life-expectancy (4,7), and health related quality of life (9). In addition to the advocation of frequent PA, there is considerable evidence supporting the inclusion of regular exercise as an adjunct therapeutic strategy in people with T1D (10). Definitively, exercise describes activities completed with a structured and intentional approach to maintaining or attaining improvements in physical fitness (11). The increased metabolic activity of skeletal muscle during exercise evokes an adaptive response of several integrative processes, including the cardio-respiratory, vascular, and metabolic systems. As a reflection of the integrative functions of these physiological systems, cardio-respiratory fitness (CRF) is often used as a barometer for physical fitness and reflects the ability of the circulatory-and-respiratory systems to supply the muscular system with the increasing oxygen (O 2 ) demand experienced during sustained PA. Although the topic remains controversial, lower CRF has been reported in individuals with-vs.-without T1D (12). However, this finding appears to be closely related to glycaemic control, which can considerably influence acute exercise tolerance (12)(13)(14). Whilst considerable shifts in both cellular and tissue homeostasis are observed following most exercise activities, these processes are magnified following higher-intensity exercise, which represents a potent physiological stress. In the long-term, this acute stressor functions to optimise the body for subsequent subjection to bouts of physiological or indeed pathophysiological insults. Therefore, the type and intensity of the exercise stimulus can considerably influence the acute physiological and chronic adaptive responses that occur.

EXERCISE DEFINITION AND CLASSIFICATION
The word "exercise" is often used interchangeably and encompasses several adjustable variables including modality, frequency, intensity, and duration. Metabolically speaking, the energy contribution to a given task is determined by the intensity of effort required for its performance. These metabolic disturbances activate several kinases and phosphatases, necessary not only for the immediate supply of energy to sustain contractional output, but also the synthesis of genetic transcriptions that produce an adaptive phenotype for subsequent functional demands. Generally, the pathways responsible for the provision of adenosine triphosphate (ATP) to skeletal muscle are categorised as aerobic i.e., with the use of O 2 or anaerobic i.e., without the use of O 2 . In each pathway, muscle must convert free adenosine diphosphate (ADP) and inorganic phosphate (Pi) to ATP. The regenerated ATP is then made available to the myosin ATPase enzymes that facilitate the contractile processes that enable movement. During low to moderate intensity exercise, the rate of O 2 supply necessary for continued contraction is comfortably met via the oxidative metabolism of carbohydrates and lipids. During intense physical exercise conditions of cellular hypoxia are experienced, as the rate of O 2 demand far exceeds the rate of supply capable via oxidative phosphorylation. Under such circumstances, anaerobic metabolism becomes the predominate means via which ATP is produced. The process involves the degradation of phosphocreatine (PCr) i.e., the ATP-CP system and the production of lactate within the glycolytic pathway i.e., the glycolytic system (Figure 1).
Essentially, a continuum exists that enables physical exercise activities to be grouped into low, moderate, and high intensity efforts. However, it should be noted that no exercise is ever considered ''exclusively" aerobic or anaerobic, rather both pathways are activated simultaneously with one predominating fuel provision. Nonetheless, the terms provide reference guides that can be used generally to classify exercise intensities. Conventionally speaking, predominately aerobic exercise imposes a high-frequency (repetition), low power output (load) demand on muscular contraction. These characteristics are often observed during MICE including endurance orientated activities. Conversely, predominately anaerobic exercise mandates a lowfrequency, high-resistance power output including both high intensity exercise (HIIE) and RE.

Resistance Exercise Definition
Resistance exercise or "strength/weight" exercise is a term used to describe exercise that mandates the body's musculature to move against an opposing force, addressing the functional behaviours mandatory to human movement. Resistance exercise training (RET) is the process of developing strength, flexibility, coordination, and stamina through performing exercises (at self-selected, high-intensity efforts) that involve multiple joints and muscle groups. RE be implemented using a multitude of training modalities, including isokinetic resistance, variable resistance, isometric resistance, and explosive plyometrics. This is important, as the term "resistance exercise" is often strictly associated with the use of equipment-based/external loads placed on the body in a strength orientated fashion. However, it actually reflects the exercise continuum-from which program design allows variable adaptation. Fundamentally the acute program variables (muscle action, intensity and volume, exercise selection and order, rest interval, repetition velocity, and frequency) dictate the specific training outcome (i.e., muscular strength, hypertrophy, maximal strength, or power). As such, there appears to be a specific relationship between the training stimulus and the adaptive response. Due to its malleability RE can be easily adapted to suit the attainment of a specific exercise goal. Indeed, small adjustments in the load, velocity and duration of a typical RE movement can substantially alter the physiological response that occurs.

Resistance Exercise Guidelines
As per the latest position statement by the American Diabetes Association (ADA), the adoption and maintenance of PA are critical foci for blood glucose management and overall health maintenance in individuals with T1D (5). Indeed, as part of their core guidelines, the ADA state that individuals with diabetes should engage in 150 min or more of moderate-to-vigorous intensity activity weekly. Guidelines suggest spreading these minutes over 3 days per week, with no more than 2 consecutive days passing without activity. Shorter durations (minimum 75 min per week) of vigorous-intensity or interval sprint training may be sufficient for younger and more physically fit individuals. Furthermore, the specific advocation of RE is included, and details that adults with T1D should engage in 2-3 sessions per week on non-consecutive days. Encouragingly, this also aligns with the latest position stand by the American College of Sports Medicine, who advocate the implementation of RE for each of the major muscle groups on ≥2 days per week in healthy adults (11). Finally, considering the elevated susceptibility to diabetes related complications, the utility, safety and physiological adaptations associated with RE are increasingly important. Due to its ability to train the peripheral muscles without producing extensive cardiovascular stress, dynamic interval based RE, using loads of 50-60% 1-repetition maximum (1RM), has been established as a safe and effective mode of exercise in high cardiovascular disease (CVD) risk cohorts (16).
Despite these advocatory guidelines, there is currently a lack research comprehensively detailing the physiological responses to RE in individuals with T1D (Table 1). Moreover, in what little RE-focused research pertinent to T1D that does exist, there is often a lack of program variability, which may overlook important responses to alterations in acute program variables i.e., time under tension, repetition ranges and exercise selection. Furthermore, existing research has mostly explored acute sessions, with participant numbers failing to exceed 15. Finally, the cohorts used have been relatively homogenous, made up of predominately young, relatively healthy, Caucasian male participants. This not only makes wider generalisation difficult, but also overlooks important gender, ethnic and agerelated variables.

MORPHOLOGICAL ADAPTATIONS-SKELETAL MUSCLE MASS: BENEFITS BEYOND SIZE
As an extremely heterogeneous tissue with immense plasticity, skeletal muscle influences locomotion, energy metabolism, thermodynamics, endocrine complexes, and cell signalling.
From a clinical perspective, the morphological adaptations induced by RET are associated with reduced rates of chronic inflammation (29)(30)(31)(32), lowered global and central adiposity (31,33), and a reduced risk of falls and fractures (34). Encouragingly, a recent article highlighted the credibility of RET in augmenting myocyte mitochondrial respiratory capacity and function (35). Considering both skeletal muscle insulin resistance and mitochondrial dysfunction are major features  in the pathogenesis of T1D (36), interventions aimed at stimulating skeletal muscle growth are increasingly important in a diabetic milieu. The importance of preserved skeletal muscle mass is perhaps most evident in reference to age related muscle wasting or "sarcopenia." This pathological derangement decreases the efficiency of several major metabolic processes, thereby accelerates the development risk of metabolic and mitochondrial abnormalities. Skeletal muscle mass is maintained when there is equilibrium between muscle protein synthesis (MPS) and muscle protein breakdown (37). A disturbance in this finite balance during which MPS exceeds the rate of protein breakdown induces skeletal muscle hypertrophy i.e., an increase in muscle size, whilst the opposite is said for atrophy i.e., a decrease in muscle size. The cellular mechanisms responsible for the initiation of growth involves the activation of various anabolic substrates including the phosphatidylinositol 3-kinase (P13K) signaling substrate mammalian target of rapamycin (mTOR) (38). mTOR is a serine/theronin kinase that integrates nutrient and metabolic stimuli to regulate cell growth and proliferation (39,40). It's activation is dependent on sequential upregulations of P13K, phosphoinositide-dependent kinase (PDK)-1, 70-kDa ribosomal S6 kinase, and the Akt/PKB isoforms (41). Metabolically, mTOR increases glycolytic flux by activating the transcription and the translation of hypoxia inducible factor 1α (HIF1α) (42), whilst Akt facilitates the stimulation of glycogen synthase and formation by inhibiting glycogen synthase kinase-3 (31). HIF1α is an intermediate of several glycolytic and angiogenic genes including both vascular endothelial growth factor (VEGF) and erythropoietin (EPO) (43). VEGF triggers not only divisional but also phenotypical changes of the vascular endothelial cells to alter their migratory capacity and proliferation potential, whilst EPO stimulates erythropoiesis and therefore systemic O 2 delivery. The hypertrophic potential of RE is not novel and its efficacy in substantially increasing rates of MPS in the many hours after exercise engagement are wellestablished (44). Biochemically, RE is known to evoke a transient increases in the phosphorylation of several of the aforementioned anabolic signalling molecules including mTOR (41), Akt/PKB (45), and 70-kDa ribosomal S6 kinase (45). Conversely, due to its inhibitory effect on several biochemical mediators of MPS, rates are minimal and/or unapparent following MICE. Encouragingly, recent research has demonstrated significant increases in upstream mediators of mTOR (growth hormone) in a volume dependent manner following acute RE in individuals with T1D (24). Since mTOR deregulation (46) and low CRF (47) are reported features of T1D, the biochemical cascades activated in response to appropriately designed RE are particularly noteworthy.

Skeletal Muscle Strength and Fatigability
The functional significance of hypertrophied skeletal muscle is reflected by the accompanied increase in maximal strength and power (48). Muscular strength describes the amount of force a muscle can produce in a single effort, whilst muscular power describes the ability of the muscle to exert that force rapidly. The development of these two physical attributes extends to beyond enhancements in athletic performance. Strong, powerful muscles are necessary for the completion of simple, everyday tasks, which translate to greater independence and autonomy with aging, lower rates of all-cause mortality, enhanced fluidity of movement, and a decreased risk of injury (49). Beyond its pivotal role in locomotion, the clinical significance of hypertrophied muscle is also noted. The associations between reduced skeletal muscle strength and higher rates of clinical biomarkers of CVD are critically important in those with T1D, who are predisposed to a greater incidence of primary CVD throughout the lifespan (50). Indeed, research has highlighted a significantly lower muscle strength and fatigability in those with-vs.-without T1D (51). A physiological explanation for these declines may be evident within the muscle fibre type, since muscle fibre arrangement and/or type has a functional significance on the rate of force production and contraction velocity. Briefly, there exist two broad classifications of muscle fibres, Type 1, and Type II (the latter of which can be further subdivided to include Type IIa and IIx variations). However, it should be noted that whilst useful, these divisions are overly simplistic and do not reflect the reality of fibre type distribution-which includes a heterogenous mix within the skeletal muscle.
Structurally, there appears to be a reduced number of slowtwitch oxidative fibres (Type 1) coupled with an increased amount of the fast-twitch highly fatigable muscle fibres (Type II) in individuals with T1D. Combined, these increase the development of fatigue during maximal exercise and therefore necessitate earlier exercise cessation (52). Supporting these insights, previous work by Crowther and colleagues evidenced a significantly lower muscle pH at rest and at the end of exercise, indicating a greater reliance on glycolytic metabolism in the muscles of individuals with vs.-those-without T1D (53). Moreover, a significantly slower phosphocreatine (PCr) recovery time has been noted in adolescence with-vs.-without T1D, suggesting a reduced skeletal muscle oxidative profile with impaired recovery capacity (36). Whilst an altered skeletal muscle phenotype has been observed in T1D, the severity of these reductions appears to be magnified by the presence of diabetes-related complications. Recent work by Orlando and colleagues illustrated an impairment in the functionality of skeletal muscle in T1D patients with diabetic polyneuropathy (DPN) vs. those with complication free T1D (T1D) and healthy controls (C) (51). Findings revealed the DNP group had lower knee extensor muscle strength than both the T1D (−19%) and the C groups (−37.5%) as well as an elevated (22 and 45%) rate of lower body fatigability than the T1D and C groups, respectively. Critically, RE induces several neural adaptations, including disinhibition of inhibitory mechanisms, as well as improvements in both intra-and-inter-muscular coordination. This enhances the synchronisation of motor unit recruitment and firing capacity to facilitate greater levels of maximal force production. These adaptations are proportional to alteration in muscle morphology as seen by increases in muscle cross-sectional area (CSA) accompanied by increases in Type II muscle fibres and altered muscle architecture (fibre pennation) (37). A large part of this process is mediated by the central nervous system which acts as a highly complex control centre that facilitates movement at the molecular level (54).

METABOLIC RESPONSES TO RESISTANCE EXERCISE
T1D is characterised by the progressive depletion and destruction of pancreatic β-cells accompanied with impaired glucagon-producing α-cell function. The resulting deficiency in endogenous insulin secretion manifests in chronic hyperglycemia, with the sequential need for a lifelong reliance on exogenous insulin therapy (55). On the other hand, hypoglycemia represents a perpetual clinical and conventional worry which independently constitutes a primary patient reported barrier to regular exercise participation (56). Therefore, a key consideration for those with T1D whom partake in any physical exercise is the maintenance of blood glucose (BG) control. However, whilst accounted for with little contemplation in regulatory metabolism, the attainment of this outcome requires a degree of diligent pre-planning in those with T1D, for whom variations in exercise modality, intensity, and duration require specific adjustments in therapeutic and feeding strategies in order to mitigate risk.

Glycaemic Responses to Resistance Exercise
The control of BG during exercise involves the integrative coordination of two physiological systems (1) the autonomic nervous system (ANS) with particular emphasis on its sympathetic branch (SNS) and (2) the endocrine system (48). At a simplistic level, biochemical mediators released via these systems provide the feed-forward mechanisms that dictate glucose homeostasis. During moderate-to-high-intensity exercise, this feed-forward mechanism induces a rise in glucose concentrations which is primarily driven by hepatic glucose production (HGP). When glucose concentrations begin to fall as a result of sustained exercise efforts, the body produces a strong counter-regulatory response in an attempt to avoid hypoglycemia. This response is driven by the release of counterregulatory hormones i.e., those that exert opposing actions to those of insulin. Recent work exploring the impact of single and multiple sets of RE on acute glycemic and glucoregulatory parameters has provided valuable endocrinological information in those with T1D (24). Researchers found significant increases in BG concentrations compared to resting conditions following one (+21%) and two (+29%) but not 3 sets of RE. The increases in BG were concurrent with a significant time * interaction effects in plasma catecholamines (noradrenaline and adrenaline). Indeed, peak catecholamine values were 28% greater under the 2nd set than the 1st set, and 17% greater under the 3rd set than the 2nd set. An increase in these two hormones parallels the idea that sympathoadrenergic activity can modulate HGP during exercise (57). Moreover, peak concentrations in growth hormone (GH) were observed during the 3rd (and final) set, which also agrees with work by Kraemer et al. who support the use of high volume session for maximising potential for hypertrophic outcomes (38). Furthermore, the considerable disturbance in acid-base balance observed during this protocol supports the "anaerobic" classification of RE that utilises predominately non-oxidative fuel metabolism via gluconeogenic pathways. Interestingly, despite the increasing hormonal and BG trajectory during the first two sets, the inclusion of a 3rd set produced BG concentrations similar to that of a non-exercise trial (24). It should also be noted that during recovery from 1 to 3 sets of RE, researchers reported no evidence of either exercise-induced hypoglycaemia or requirement for exogenous carbohydrates (CHO) (24). These findings oppose those commonly associated with AE, which typically reduces BG concentrations to a much greater extent (28,58). Physiologically, the metabolic consequences of nonoxidative glycolytic activity likely contribute to the associated elevations in BG during the post exercise period, thereby offering protection against the onset of post-exercise hypoglycaemia. As a cautionary note, recent research has illustrated an increase in meal intake and after dinner snacking during the 24 h following both aerobic-and-resistance exercise sessions in order to prevent hypoglycaemia (28). Notably, findings highlighted significant reductions in post RE bolus insulin dosing requirements, which were also accompanied by less severe drops in plasma BG concentrations comparative to those recorded following AE (28). Accordingly, current recommendations are to reduce rapid acting insulin dosages alongside side a CHO rich preexercise meal (59,60). This reductive strategy offsets the degree of hyperinsulinemia in people with T1D, which constitutes an inevitable outcome associated with exogenous insulin administration. Metabolically speaking, hyperinsulinemia exerts an inhibitory effect on fat utilisation during exercise, with the subsequently overreliance on CHO oxidation and therefore glucose dependency by the exercising skeletal muscle. Moreover, irrespective of interventional dose manipulations, insulin concentrations often increase during exercise (61). Mechanistically, this is likely due to an increased absorption rate from the subcutaneous tissue depot, together with a decreased clearance rate from at the systemic level. Combined the elevated degree of concentration of circulating of insulin propagates an increased risk of exercise-induced hypoglycaemia, which unsurprisingly prevails as a major barrier to exercise participation (56). Previous work has shown that postprandial exercise is associated with an increased risk of hypoglycemia if the pre-meal insulin dose is not reduced (62). Physiologically, this dose reduction strategy can augment the clearance rate of BG following the digestion of a CHO rich meal, thereby preserving BG concentrations ahead of exercise (62). The avoidance of in-exercise hyperinsulinemia has also been shown to replicate patterns of substrate oxidation and glucose regulation similar to a non-T1D individual (61). Moreover, a reduction in insulin mediated glucose uptake, in combination with elevations in catecholamine concentrations lessens the uptake and subsequent combustion of BG. Consequently, the major fuel substrates can be provided for both HGP and replenishment of muscle glycogen stores (63), such that a preservation effect is observed (64). Directionally, the cost of a slightly higher plasma glucose level before exercise translates to an improved glycemic profile during-and immediately-post-exercise (62).
It should be noted that when the exercise session is predominately reliant on non-oxidative fuel metabolism, postexercise hyperglycaemia is common (23). The degree to which BG concentrations rise is dependent on several variables and can differ considerably both within and between individuals. Work by Turner and colleagues noted that when matched for volume, a comparable degree of post exercise hyperglycaemia is experienced in both low-and-high intensity morning RE sessions (26). Subsequent work highlighted the efficacy of administering an individualised dose of post-exercise rapid acting insulin in reducing the magnitude and severity of post-exercise hyperglycaemia, without causing early postexercise hypoglycaemia in those with T1D (65). However, this protective effect waned thereafter, when prevalence rates of hypoglycaemia ensued during the later hours (65). Research has also demonstrated that the omission of pre-exercise bolus, but continuation of basal insulin dosages offers a protective effect against the occurrence of hypoglycaemia during and soon after a fasted bout of morning RE. A direct comparison between glycaemic responses to evening AE and RE in T1D revealed a decline in plasma glucose during a three-set evening RE session (albeit to a lesser extent than observed in the AE trial). Notably, there was also a tendency towards more frequent nocturnal hypoglycaemia following RE which warrants acknowledgment when considering the implementation of post exercise insulin dosing strategies to avoid acute hyperglycaemia. The differences in methodology between these two studies provides potential clues for contrasting glycaemic responses. Nevertheless, the authors concluded that in comparison to AE, RE may result in greater glycaemic stability both during and after exercise in those with T1D (20). Collectively, these data emphasise the need to account for exercise modality for the governance of appropriate insulin and feeding strategies around physical exercise in people with T1D.

Relationships Between Skeletal Muscle, Obesity, and Insulin Resistance
The fact that skeletal muscle tissue is one of the largest organs in the human body, combined with the discovery that contracting skeletal muscle is a multifactorial control centre for the release of various genetic, cytokine and growth-related factors, supports the reasoning behind it's classification as an endocrine organ (31). Due to its mediatory role in promoting skeletal muscle vitality, integrity, and functionality, the potential of RE in influencing the progression or regression of several pathogenic disease states should not be overlooked. Although not historically a condition associated with obesity, there is an alarming percentage of individuals with T1D now categorised as either overweight or obese (66). Whilst intensive insulin therapy is often advocated to counteract poor glycaemic control, aggressive treatment can lead to weight gain, with the subsequent propensity to develop insulin resistance (IR). Worryingly, IR has become a recognised featured of T1D youth, accompanied by compromised exercise capacity and microvascular derangements (36). Moreover, in a study assessing the hemodynamic parameters to maximal exercise in patients with well-controlled T1D, researchers found IR to be inversely associated with cardiac output (67). IR occurs when an inadequate quantities of glucose transporter molecules (namely GLUT-4) migrate to the cell surface following insulin stimulation (31). The translocation site of GLUT-4 is the T-tubules found within the skeletal muscle, which highlights the potential of contracting skeletal muscle to uptake circulating glucose independently of insulin. Thus, whilst not novel, the "insulinlike" properties of skeletal muscle are extremely important in pathologies with metabolic abnormalities. As a fuel source, fat constitutes the most abundant energy pool in mammals and is stored in three major sites; adipose, skeletal muscle, and liver tissue. Each tissue contains depots of triacylglycerol molecules which are hydrolyzed to form fatty acids (FAs) and used as an energy source (31). However, an excessive accumulation of triacylglycerol leads to conditions of hyperlipidemia, with the subsequent propensity to develop IR (68). Skeletal muscle FA metabolism is acutely sensitive to physical exercise which causes substantial increases in intra and extra-cellular FA utilisation (31). Skeletal muscle is a leading consumer of FA from either lipoprotein-triacylglycerol or plasma non-essential fatty acids, and this consumption process rises exponentially during exercise. Finally, as a metabolically active tissue, skeletal muscle plays a pivotal role in maintaining body composition. Acutely, the prolonged window of increased MPS following RE induces elevations in thermogenesis and energy expenditure. Considering that skeletal muscle accounts for ∼40% of total body mass and ∼30% of basal metabolic rate (BMR) in adult humans (40), hypertrophied skeletal muscle may elevate resting muscle metabolism due to an increased ratio of skeletal muscle mass relative to body weight. Therefore, appropriately programmed exercise programmes that stimulate skeletal muscle hypertrophy may assist in weight reduction and/or management in those with T1D.

CARDIO-VASCULAR RESPONSES TO RESISTANCE EXERCISE
For individuals with T1D, CVD is a major cause of morbidity and mortality and unsurprisingly constitutes a serious health concern that demands a prevention rather than treatment approach (69)(70)(71). Encouragingly, recent research has acknowledged the credibility of RE in reducing several CVD risk factors as well as improving various biomarkers of cardio-metabolic health (albeit it in a non-T1D cohorts) (72,73). Although sparse, research has emphasised the efficacy of RET in improving traditional indices of CVD in people with T1D, including glycaemic and lipid related parameters (17). Furthermore, increases in heart rate (24,26) and forearm reactive hyperaemia (25) have been reported following RE in individuals with T1D. Physiologically, the hypertrophic and angiogenic potential of RE is perhaps magnified in importance during circumstances where blood flow maybe compromised due to pathological abnormalities. Supporting this concept, researchers observed greater improvements in blood flow following lower limb RE vs. AE in individuals with T1D (25). However, these peripheral based improvements have thus far failed to correspond with improvements in cellular indices of vascular function. Indeed, a blunted circulating endothelial progenitor cell (cEPC) response has presented regardless of exercise modality, perhaps reflecting derangements in the microvascular domains. Although it should be noted that existing literature has thus far employed mostly MICE (74) and/or submaximal-lower limb RE (25).

SKELETAL MUSCLE: IMPORTANT MICRO-VASCULAR AND ANGIOGENIC POTENTIALS
Prevalence rates of circulatory disease in people with T1D are extensive and at a population level constitute a leading cause of mortality (50). The pathophysiological mechanism responsible for the compromised circulatory system is most evident by the presence of abnormalities in the form and function of the blood vessels (50,75,76). Thus, whilst the identification of classical CVD risk factors provides valuable information as to an individual's current CVD vulnerability, they often fail to identify risk in its infancy and/or in those who appear to be asymptomatic. As such, physiological defects can go unrecognised until manifested as irreversible and/or severe acute complications within blood vessels including the presentation of endothelial dysfunction. Whilst the pathogenesis of macro-and-micro-vascular disease is multifactorial, the common recipient of injury is the vascular endothelium; a monolayer of cells that lines the blood vessels. Occupying a strategically important location between circulating blood and the surrounding tissues, the endothelium modulates the tone of the underlying vascular smooth muscle, maintains a non-adhesive luminal surface, mediates homeostasis, evokes cellular proliferation, and modulates inflammatory and immune mechanisms within the vascular wall (77). The functionality of the endothelium is mediated by a tightly regulated balance between the intermediates that induce cell damage i.e., pro-constrictive, pro-inflammatory, pro-thrombotic, and prohypertensive factors and the intermediates that support cell vitality i.e., pro-relaxation, anti-inflammatory, anti-thrombotic, and anti-hypertensive factors. Skeletal muscle hosts several sources of these vitality promoting intermediates, including the erythrocytes (which produce ATP and Nitric Oxide [NO]), the endothelial cells (which produce ATP, NO, and Prostacyclin [PGI 2 ]), the skeletal muscle cells (which produce ATP, NO, and adenosine). Thus, during skeletal muscle contraction, there is an increased turnover of various vasodilatory factors that encourage blood flow and vascular repair processes. Indeed, the co-operative intimacy between skeletal muscle and its surrounding vasculature is made clear by the proximity of capillaries embedded in grooves indenting the sarcolemma and expanding the contact area between myocytes and endothelial cells (78). Muscle fibre mitochondria also appear to cluster along these groves, thereby shortening the travelling distance of O 2 and various nutrients required for diffusion (78). The complex and intermate interaction between the biochemistry of the vascular endothelium, the vascular smooth muscle cells (VSMCs) and the skeletal muscle fibres emphasises the importance of endothelial vitality. Endothelial derangements proposedly contribute to the chronic under perfusion of skeletal muscle, resulting in suboptimal delivery and uptake of various nutrients, hormones and gaseous exchange. Indeed, these findings are somewhat consistent within the literature with research detailing higher rates of leg muscle deoxygenation (79), and altered red blood cell dynamics associated with the T1D milieu (80).
Mechanistically, microvascular abnormalities may be a result of lower skeletal muscle capillary density in individuals with T1D (81) which would agree with the aforementioned muscle fibre phenotype associated with T1D. Collectively, these attributes may contribute to a reduced circulatory ability to increase O 2 delivery to exercising muscle. This may explain the lower rates of regular physical activity (56) as well as the earlier onset of fatigue observed in T1D during exercise testing where O 2 delivery contributes to fatigue (82). Inefficient O 2 and nutrient supply may also impact the vascular tone of skeletal muscle. Vascular responses to light exercise provide insight into the hierarchical regulation of muscle perfusion. With light exercise, vasomotion within the terminal arterioles appears to cease, and the number of perfused capillary increases without significant changes in total muscle blood flow. Thus, during MICE, there is less of an increase in shear stresses traversing over the vessel lumen than during higher intensity exercise. During higher intensity exercise, or following ischemia, underperfused capillaries are recruited to ensure an increase muscle perfusion. Indeed, in people with T1D, 7 weeks of highintensity sprint training has been shown reduced metabolic fluctuations and enhance muscle oxidative metabolism (83). Thus, as a major storage site of several nutrients including glucose, amino acids, and free fatty acids, skeletal muscle also plays an integral role in substrate provision during hypoxic or anaerobic conditions. These insights suggest a credible role for exercise modalities that utilise compound movements performed at high intensity effort as a strategy for re-endothelisation. Perhaps, as a reflection of its capacity to induce ischemia and muscle microtrauma, exhaustive or eccentric exercise protocols including downhill running and RE appear to cause the greatest stress responses. The acute physiological "stress" experienced during these sessions augments the production and release of several mediators of reendothelisation including stromal cell-derived factor 1 (SDF-1) (84) and VEGF (85). Upon activation, these proteins mediate progenitor cell mobilisation, proliferation, and migration (86). One of the most potent upstream mediators of EPC proliferation is VEGF, which is primarily known for its mediatory role in the (i) sprouting and (ii) intussusceptive splitting of capillary beds to form angiogenesis. Recent research has shown considerable elevations in VEGF concentrations within both skeletal muscle and the peripheral circulation following intense RE (87,88). As such, the potential of exercise to induce local angiogenesis may assist in the prevention of ischemia within its surrounding tissue (43). Considering the decreased perfusion rates and increased atherosclerotic tendencies associated with T1D, the angiogenic potential of RE in this population is important.

OSTEOPATHIC RESPONSES TO RESISTANCE EXERCISE
The term "osteoporosis" describes inelasticity within the skeletal system when exposed to the habitual subjection of forces experienced during joint movement, locomotion, and loading. Bone mineral density (BMD) is used as a reference of bone mineralisation within bone tissue and is substantially influenced by endocrine activity. The endocrine and metabolic disturbances associated with T1D are hypothesised to contribute to higher rates of poor bone quality noted within the literature (89)(90)(91). Biochemically, T1D is associated with reductions in the anabolic agents of bone formation including IGF-1 and transforming growth factor beta 1 (TGFβ1), which initiate a potent stimulatory effect on the synthesis of bonespecific proteins and osteoblastic proliferation potential (92). Moreover, the downstream complications associated with hyperglycaemia including the direct inhibition of osteoblast activity, osmotic damage to osteoblasts, increased peroxisome proliferator-activated receptor activity and low microvascular proliferative capacity, may increase susceptibility to osteoporosis and fracture risk.
Physiologically, bones adapt to loading stimuli by upregulating osteoblast activity in the areas experiencing mechanical strain. With this in mind, the essence of RE may offer support against the poor bone quality sometimes observed in T1D. Supporting this concept, T1D centred paediatric research noted increases in both lean body mass (LBM) and BMD following 9-months of weight bearing activity (93). These physiological adaptations are mutually connected, since there seems to be a stoichiometric relationship between muscle and bone. Indeed, bone architecture depends critically on muscle CSA and tension development (94), whilst muscle immobilization, and/or detraining leads to bone mass loss (95). Moreover, exercises that require large rates of muscular force cause greater bone-related benefits than do isometric or low force exercises (94,(96)(97)(98). Conversely, extreme endurance and/or low load bearing activities may exasperate the degradation of bone quality and health (99,100). Researchers have documented losses in bone mass during the competitive seasons in endurance cycling (100) whilst increases in bone mass are ascertained during the competitive seasons of gymnastics (95). Further research is needed to progress our understanding of the osteopathic responses to RE in individuals with T1D.

RECOMMENDATIONS FOR RESISTANCE EXERCISE IN TYPE 1 DIABETES
There are several acute manipulations which can substantially influence the outcome induced by RE. Indeed, DeLorme's classic work suggested that RET using low repetition/high resistance favoured adaptations for strength, power, and hypertrophy, whereas training with high repetition/low resistance increased muscular endurance and oxidative potential (101). From this, a repetition training continuum (102) or repetition maximum continuum (103) has been hypothesised such that the number of repetitions allowed by the resistance will result in very specific training adaptations. In order to enable a clear outline of program design, early work by Bird et al. (104) collated this information and created a visual representation of RE prescription. A modified version of this template that emphasises the points relevant to this review is included below (Figure 2).
Considering the focus of this review has emphasised the importance of skeletal muscle size the practical recommendations to follow will reflect the attainment of muscular hypertrophy. Skeletal muscle experiences the increases in size and strength when forced to contract at maximal or near-maximal efforts. Indeed, the rate of MPS is driven by the intracellular availability of amino acids-a factor which is influenced directly by the intensity and duration of muscle tension. With overall session intensity in mind, early research by Kramer and colleagues concluded that the combined effects of a higher volume, moderate intensity session with shorter rest periods provides a favourable hormonal milieu for promoting skeletal muscle growth (38). Thus, to maximise intensity, rests between exercises and sets should therefore be kept minimal (30-60 s) to maintain an elevated skeletal O 2 demand that requires the use of predominately non-oxidative fuel metabolism.
Furthermore, the duration of muscular contraction significantly contributes to the phenotypic response. This process describes the "tempo" or time of the muscular contraction and is typically expressed in seconds. Moderate (2 s CON; 2 s ECC) and fast (1 s CON; 1 s ECC) velocities have been shown to maximise hormonal responses (105) and result in substantial metabolic cost (106). Classically the optimal repetition range for muscular hypertrophy is between 8 and 12. Initially, 2-3 sets may be enough for the untrained individual, however this should progress to >3 sets as with progressive overload. Moreover, programs that demand the simultaneous recruitment of large muscle groups traversing multiple joints in both the lower and upper body segments are advised. In this context, a program that emphasises compound movements towards the start of the session (when the muscle is in a "rested" state so can produce maximal force without fatigue), with isolated work grouped towards the end of a session is advised. In terms of exercise frequency, recommendations provided by the ADA (5) state that RE sessions aimed at skeletal muscle hypertrophy should be performed a minimum of twice but ideally thrice weekly.
Undertaking divergent exercise i.e., RE and MICE in close proximity may cause an "interference" effect on several of the acute molecular responses that orchestrate the adaptive phenotype. For example, activation of AMPK signalling in response to endurance exercise can block or dampen rates of MPS via its inhibitory effect on translation initiation and elongation (107). Moreover, the activation of AMPK is augmented in response to nutrient stress; particularly glycogen depletion; emphasising the need to acknowledge dietary considerations around exercise sessions. Thus, in an attempt to maximise MPS, individuals may wish to avoid both energy stress (increases in carbohydrate and amino acid feeding around exercise) and the concurrent practice of endurance and resistive exercise in close proximity (108). Clearly exercise carries impactful glycaemic connotations for individuals with T1D. However, factors including the type, intensity and duration of exercise as well as the level of onboard exogenous insulin can considerably affect the glycemic responses that occur. Due to the pharmacological administration of exogenous insulin therapy, people with T1D frequently engage in exercise under markedly hyperinsulinemic conditions, thus are exposed to a greater risk of hypoglycaemia (62). As such, exercise often poses a glycaemic challenge which must be performed with a degree of contingency planning to both lifestyle and therapeutic strategies. It is prudent to suggest than insulin reductions and/or carbohydrate intake in the pre-and-post exercise periods are important features that need careful consideration with reference to BG values to appropriately manage glycemia. Exercise while fasting may produce a lesser decrease or indeed smaller increase in BG (65). It is advisory that BG is checked frequently in the hours pre, during and post exercise in order to govern safe and effective decisions regarding CHO feeding and insulin administration (5). RE induced hyperglycaemia risk can be abated by interspersing intense lifting efforts with lower intensity aerobic efforts (109) and/or combing RE (completed first) with AE (completed second) to optimize glycaemic stability (110). Further studies are needed to investigate the glycaemic impact of alterations in rapid-acting insulin strategies according to the type, intensity, and duration of the intended RE session.

FUTURE RESEARCH
Intuitively, the implementation of RE, a predominately glycolytic exercise that can be programmed to achieve global conditioning characterises a compelling therapeutic strategy that can be employed to improve several complications associated with T1D. However, despite the wider health merits associated with RE, a clear research emphasis has been placed on MICE with glycemia as its primary outcome.
In the existing RE specific research, program design has orientated around primarily hypertrophy or strength related outcomes, with less work investigating muscular endurance and/or power regimes for raising daily background levels of physical activity. In light of recent research emphasising the angiogenic potential of muscular endurance resistance exercise (MERE); during which healthy participants exercised to-or-nearto muscular failure (88), there presents a compelling rationale to employ these strategies in T1D by means of augmenting skeletal muscle angiogenesis. Indeed, the angiogenic and re-endothelialisation potential of MERE is particularly noted in this patient cohort, for whom circulatory complications are frequently observed (50). Moreover, considering the aforementioned alterations in the bioenergetics of skeletal muscle associated with T1D, the neuromuscular adaptations associated with moderate-to-heavy strength RE which incorporates a mixture of isometric and isokinetic movements i.e., greater motor unit recruitment, increased discharge rate modulation, larger muscle cross section area and changes in muscle architecture, are duly noted. Future research that explores the physiological responses to RE that varies in several of the aforementioned acute and chronic training variables would not only deepen our understanding from a research perspective but offer much clinical and patient relevance that may help develop healthcare guidelines specific to those with T1D.

CONCLUSION
The importance of skeletal muscle strength and size extends far beyond it aesthetic and/or athletic performance value. Indeed, skeletal muscle plays an indispensable role in several key physiological processes including locomotion, hemodynamics, thermodynamics, cell signaling, and energy metabolism. Thus, the clinical merit of strategies that assist the maintenance and/or development of skeletal muscle are deserving of wider healthcare attention in patient populations. As a key determinant of muscle size and strength, the rate of MPS is critical for the development and/or preservation of skeletal muscle mass. This review has highlighted the potential of RE in upregulating the activity of several key biochemical and molecular signalling pathways that augment rates of MPS, including the PI3K/Akt/mTOR signalling pathway. Perhaps most importantly, the wider metabolic, vascular and respiratory roles of skeletal muscle each have particular relevance for individuals with T1D, whom are predisposed to greater pathological risk within these systems by virtue of the condition. Thus, the positive, adaptive health benefits associated with the hypertrophic and strength benefits accompanied with RE should be promoted as a necessary adjunct to standard diabetes care.