Redox Epiphospholipidome in Programmed Cell Death Signaling: Catalytic Mechanisms and Regulation

A huge diversification of phospholipids, forming the aqueous interfaces of all biomembranes, cannot be accommodated within a simple concept of their role as membrane building blocks. Indeed, a number of signaling functions of (phospho)lipid molecules has been discovered. Among these signaling lipids, a particular group of oxygenated polyunsaturated fatty acids (PUFA), so called lipid mediators, has been thoroughly investigated over several decades. This group includes oxygenated octadecanoids, eicosanoids, and docosanoids and includes several hundreds of individual species. Oxygenation of PUFA can occur when they are esterified into major classes of phospholipids. Initially, these events have been associated with non-specific oxidative injury of biomembranes. An alternative concept is that these post-synthetically oxidatively modified phospholipids and their adducts with proteins are a part of a redox epiphospholipidome that represents a rich and versatile language for intra- and inter-cellular communications. The redox epiphospholipidome may include hundreds of thousands of individual molecular species acting as meaningful biological signals. This review describes the signaling role of oxygenated phospholipids in programs of regulated cell death. Although phospholipid peroxidation has been associated with almost all known cell death programs, we chose to discuss enzymatic pathways activated during apoptosis and ferroptosis and leading to peroxidation of two phospholipid classes, cardiolipins (CLs) and phosphatidylethanolamines (PEs). This is based on the available LC-MS identification and quantitative information on the respective peroxidation products of CLs and PEs. We focused on molecular mechanisms through which two proteins, a mitochondrial hemoprotein cytochrome c (cyt c), and non-heme Fe lipoxygenase (LOX), change their catalytic properties to fulfill new functions of generating oxygenated CL and PE species. Given the high selectivity and specificity of CL and PE peroxidation we argue that enzymatic reactions catalyzed by cyt c/CL complexes and 15-lipoxygenase/phosphatidylethanolamine binding protein 1 (15LOX/PEBP1) complexes dominate, at least during the initiation stage of peroxidation, in apoptosis and ferroptosis. We contrast cell-autonomous nature of CLox signaling in apoptosis correlating with its anti-inflammatory functions vs. non-cell-autonomous ferroptotic signaling facilitating pro-inflammatory (necro-inflammatory) responses. Finally, we propose that small molecule mechanism-based regulators of enzymatic phospholipid peroxidation may lead to highly specific anti-apoptotic and anti-ferroptotic therapeutic modalities.

A huge diversification of phospholipids, forming the aqueous interfaces of all biomembranes, cannot be accommodated within a simple concept of their role as membrane building blocks. Indeed, a number of signaling functions of (phospho)lipid molecules has been discovered. Among these signaling lipids, a particular group of oxygenated polyunsaturated fatty acids (PUFA), so called lipid mediators, has been thoroughly investigated over several decades. This group includes oxygenated octadecanoids, eicosanoids, and docosanoids and includes several hundreds of individual species. Oxygenation of PUFA can occur when they are esterified into major classes of phospholipids. Initially, these events have been associated with non-specific oxidative injury of biomembranes. An alternative concept is that these post-synthetically oxidatively modified phospholipids and their adducts with proteins are a part of a redox epiphospholipidome that represents a rich and versatile language for intra-and inter-cellular communications. The redox epiphospholipidome may include hundreds of thousands of individual molecular species acting as meaningful biological signals. This review describes the signaling role of oxygenated phospholipids in programs of regulated cell death. Although phospholipid peroxidation has been associated with almost all known cell death programs, we chose to discuss enzymatic pathways activated during apoptosis and ferroptosis and leading to peroxidation of two phospholipid classes, cardiolipins (CLs) and phosphatidylethanolamines (PEs). This is based on the available LC-MS identification and quantitative information on the respective peroxidation products of CLs and PEs. We focused on molecular mechanisms through which two proteins, a mitochondrial hemoprotein cytochrome c (cyt c), and non-heme Fe lipoxygenase (LOX), change their catalytic properties to fulfill new functions of generating oxygenated CL and PE species. Given the high selectivity and specificity of CL and PE peroxidation we argue that enzymatic r e a c t i o n s c a t a l y z e d b y c y t c / C L c o m p l e x e s a n d 1 5 -l i p o x y g e n a s e / phosphatidylethanolamine binding protein 1 (15LOX/PEBP1) complexes dominate, at least during the initiation stage of peroxidation, in apoptosis and ferroptosis. We contrast cell-autonomous nature of CLox signaling in apoptosis correlating with its anti-inflammatory functions vs. non-cell-autonomous ferroptotic signaling facilitating pro-inflammatory (necroinflammatory) responses. Finally, we propose that small molecule mechanism-based regulators of enzymatic phospholipid peroxidation may lead to highly specific antiapoptotic and anti-ferroptotic therapeutic modalities.
Keywords: regulated cell death, apoptosis, ferroptosis, phospholipid peroxidation, redox lipidomics, cytochrome c, cardiolipin, lipoxygenase The real reason for not committing suicide is because you always know how swell life gets again after the hell is over.
Ernest Hemingway If you're going through hell, keep going. Winston Churchill

SIGNALING BY POLYUNSATURATED LIPIDS: AUTOCRINE, PARACRINE, AND ENDOCRINE TYPES
Billions of years of evolution created and optimized mechanisms for efficient translation of genomic information into thousands of finely tuned protein machines (1) and perfected functional interactions between the proteins through sophisticated multi-leveled signaling systems (2). Lipids of biological membranes constitute a critical part of this complicated signaling network (3). The metabolic coordination requires that the flow of signaling information proceeds with optimized levels of fidelity and speed. Conservative estimates indicate that the number of proteins in the human proteome is on the order of 10 5 -10 6 (4, 5). Thus it is not surprising that the diversity of signaling lipids coordinating multiple protein-protein and lipid-protein interactions within and between subcellular organelles, cells, and tissues may be even greater resulting in the possible >10 6 of individual molecular species in the lipidome. Engagement of membrane phospholipids (PLs) in the signaling process occurs via their biochemical modifications leading to the appearance of small amounts of "unusual" PL molecules such as their hydrolysis or peroxidation products (6). This review is focused on oxidatively modified (phospho)lipids as the signaling entities. Among them are well known lipid mediators represented by oxygenated free polyunsaturated fatty acids (PUFA) as well as oxygenated PUFA esterified into different classes of membrane phospholipids (PLs). The latter group will be the subject of the current review.
Cultural beliefs of successful societies have led to the common opinion that suicidal elimination is not the necessary way to resolve life conflicts that may encompass transient dark episodes within an otherwise bright present and even more wonderful future. This optimistic view has been expressed in many statements by politicians, writers and other artistic celebrities (including those by W. Churchill and E. Hemingway quoted above). On the molecular level, however, the ruthlessness of life/ death elimination decisions is frequently a necessary attribute of the high fidelity of cell populations and their adaptive adjustments. The suicidal programs of cell death are genetically pre-determined and deciphering their specific mechanisms represents one of the emerging fields of cell biology. The lipidderived signals may act within a given cell (autocrine signaling), affect cells within the surrounding neighborhood (paracrine signaling) or act on remote targets using the circulatory system for the transportation of death signals (endocrine signaling). Currently, more than a dozen regulated death programs have been identified in cells that accumulate excessive amounts of the (geno) toxic materials and hence are recognized by the surveillance machinery as irreparably damaged. It is believed, but not proven, that peroxidation of polyunsaturated lipids (PUFAlipids) has been associated with the initiation and execution of many, if not all, of these programs (7). In spite of these general associations, neither the specific roles of peroxidized PLs in the fulfillment of the programs nor their chemical identity have been identified. Notable exceptions are apoptosis and ferroptosis, two programs for which the progress in redox lipidomics has resulted in the deciphering of death signals. hydrocarbon chains and water-soluble polar heads. The hydrophobic/hydrophilic balance of phospholipids dictates the organization of the bilayer in which lipophilic chains interact with each other while the polar head-groups are localized at interface with the aqueous phase. In PLs, the chains represent fatty acids covalently attached to two sites of the glycerol backbone whereas the third position is occupied by phosphobase that may be a non-charged zwitter-ion (when the negative charge of the phosphate is compensated by a positive charge) or carry a negative charge (when an extra negatively charged group is present). Fatty acyls of PLs may have no double bonds (saturated) or contain one (mono-unsaturated) or several methylene-interrupted double bonds [polyunsaturated PLs (PUFA-PL)]. While unsaturated PLs may be biosynthesized both in anaerobic and aerobic conditions, the huge diversity of PUFA-PLs is characteristic for different domains of aerobic life (8).
One of the most popular concepts explaining the presence of diversified PUFA-PLs in the lipid bilayer relates to their function as a regulator of membrane fluidity necessary for the rapid diffusion and conformational flexibility of membrane proteins (9). In spite of its attractive simplicity, this concept does not explain the huge molecular variety of PUFA-PLs. Indeed, contemporary lipidomics detects 10 3 -10 4 individual molecular species of major classes of phospholipids in cells and tissues. This is a conservative estimate of the species with differing masses. Indeed, with the two acyls/PL molecule and a menu of >30 commonly found fatty acids the number of possible isomeric species of "two-legged" PLs should be close to 10 3 . However, for "three-legged" tri-glycerides this estimate would yield 10 4 species and for "four-legged" mitochondrial cardiolipins (CL) ->10 5 molecular species.
One of the prominent features of PUFA-PLs is their susceptibility to peroxidation via free radical mechanisms (10). These mechanisms may be comprised of enzymatic systems for the activation of oxygen and/or lipid substrates or occur non-enzymatically (see below). As the general schema of peroxidation includes abstraction of hydrogen from bis-allylic positions, PLs with multiple (four-six) double-bonds, are preferred substrates, particularly for non-enzymatic free radical reactions (10,11). The primary product of the peroxidation process generates hydroperoxy-PLs (HOO-PLs). These products are not stable and readily undergo secondary decay reactions leading to a variety of electrophilic aldehydic-, keto-, hydroxy-derivatives as well as cyclic compounds (10). In terms of lipid diversification, this adds another order of m a g n i t u d e t o t h e p o s s i b l e n u m b e r o f i n d i v i d u a l phospholipids, thus bringing it to 10 5 species. It should be noted, however, that the measurable amounts of peroxidized PLs in healthy cells and tissues is markedly lower than their non-oxidized parent PLs (12). This is partly due to the fact that only a fraction of PUFA-PLs are involved in peroxidation and also to the high reactivity of the secondary electrophilic decay products toward nucleophilic sites in proteins (13). As a result, the life-time of these products in "free" form may be relatively short as they form lipid-protein adducts. However, the levels of these products may increase many-fold in conditions associated with cell injury and death. These reactive secondary intermediates of PUFA-PL peroxidation represent the proximate entities affecting functions of numerous proteins (14). Given that the formation of these adducts is, in a way, a reaction of protein "lipidation" that may dramatically change the distribution and functional characteristics of the affected proteins, it has been hypothesized, although not proven, that electrophilic products of PL peroxidation and their adducts with specific proteins represent the proximate "gateways" of cell's demise in regulated cell death programs.

TYPES OF REGULATED CELL DEATH: INVOLVEMENT OF LIPID PEROXIDATION AND POSSIBLE INVOLVEMENT OF PL-OOH
Since the time of the first detailed description of regulated cell death almost five decades ago, about a dozen different programs have been identified (15). The best described programs include apoptosis, necroptosis, pyroptosis, ferroptosis, entotic cell death, netotic cell death, parthanatos, lysosome-dependent cell death, autophagy-dependent cell death, alkaliptosis, and oxeiptosis (15). The majority of them have been qualified as responses to different types of stresses causing irreversible changes not only to one particular cell but also representing a high-risk threat to the entire community of surrounding cells. Among the different causative factors, oxidative stress has been universally identified as one of the leading mechanisms engaged early at the initiation or later during the execution stages of the death programs (15) ( Table 1).
Given the vague definition of what exactly "oxidative stress" means, attempts have been made to connect the death programs with specific pathways and manifestations of the aberrant redox metabolism. Due to the high sensitivity of PUFA-PL to oxidative modifications, lipid peroxidation (LPO) has been considered as one of the common denominators of programmed cell death (57,58). However, the specific role and mechanisms of LPO in the pathways leading to cell demise remain poorly defined. This is due, to a large extent, to difficulties in the analysis of highly diversified and very low abundance LPO products (12). These technological problems were resolved with the advent of highresolution liquid-chromatography-mass spectrometry (LC-MS) based redox lipidomics (6,12) with its capability to detect, identify and quantitatively characterize a variety of PL oxidation products. While the application of this technology may provide important information in any of the known death pathways, so far the significant results have been obtained mostly for two death programs-apoptosis and ferroptosis (16,21,34,36).
Numerous studies of PL peroxidation in model chemical and biochemical systems using different initiating agents (e.g., azo-initiators of peroxyl radicals, Fe-ascorbate dependent generators of HO• radicals) demonstrated that the susceptibility to oxidative modification is largely defined by the number of double bonds in the fatty acid residues (59). As a result, PLs with hexa-, penta-, and tetra-enoyl residues are the predominant peroxidation substrates as compared to doubly or triply unsaturated PLs. Notably, the nature of the polar part of the PL molecules was not influential as a factor determining vulnerability to oxidation. In sharp contrast, redox lipidomics studies of programmed death associated peroxidation has determined that there is a high selectivity of the process toward specific classes of PLs. Execution of the intrinsic apoptotic program revealed a high selectivity toward peroxidation of mitochondrial CLs whereby the species with C18:2 represented the major substrates (16,21). Notably, CL molecular species containing more PUFA residues remained non-oxidized. Moreover, in hetero-acylated CL species, oxidation of C18:2 residues occurred preferentially even when C22:5 and C22:6 residues remained non-oxidized within the same molecule ( Figure 1).
Ferroptosis-associated LPO was also highly selective toward a specific PL class-PUFA-containing phosphatidylethanolamines (PE) (34). Interestingly, two types of PE-molecular species with C20:4 and C22:4 displayed the highest sensitivity toward oxidative modification. In terms of positional specificity, the 15 th position in C20:4 and the 17 th position in C22:4 were the preferred oxidation sites. Importantly, these PE oxidation products exerted predictive features of ferroptosis biomarkers and displayed pro-ferroptotic activity upon co-incubations with target cells (34). A recent study demonstrated that not only diacyl-phospholipids but also PUFA-plasmalogens (etherphospholipids), synthesized in peroxisomes, underwent peroxidation in ferroptosis (60). Downregulation of ether phospholipids was associated with the increased resistance of cancer (carcinoma) cells to ferroptosis in vivo.
Selectivity and specificity of the PL peroxidation process in two different cases of apoptotic and ferroptotic (non-apoptotic) regulated cell death suggest a possible involvement of enzymatic catalytic mechanisms. Indeed, two different metalloproteins, a hemoprotein cytochrome c (cyt c) ( Figure 2A) and a non-heme Fe-protein, 15-lipoxygenase (15LOX) ( Figure 2B), have been identified as the highly likely enzymes initiating the peroxidation process in apoptosis and ferroptosis, respectively (16,34). In both cases, the selectivity of the enzymatic peroxidation mechanisms is achieved due to the formation of lipid-protein or protein-protein complexes as described below.  Wiernicki et al. (7) g-Irradiation, g-IR; staurosporine, STS; actinomycin D, ActD; nonyl-acridine orange, NAO; acute kidney injury, AKI; buthionine sulfoximine, BSO; imidazole ketone erastin, IKE; traumatic brain injury, TBI; hepatic ischemia-reperfusion injury, HIRI; cerebral ischemia, CI. PUFA residues of lipids are believed to be highly susceptible to oxidative modification by oxygen (10,64). This process, LPO, proceeds via the formation of radical intermediates. The rate limiting stage is the initial formation of radicals that can further propagate the overall process. Therefore, the peroxidation rate is very low in the absence of catalysts. While there are many different radical initiators-physical factors like irradiation or chemical agents, like compounds spontaneously decomposing to  (36) is shown in ribbon diagram. The PEBP1 (shown in cyan) is docked onto the 15LOX, and this complex model, was used to dock HpETE-PE molecule (shown in pink). The ligand docking for both cyt c and 15LOX/PEBP1 complex was performed by SMINA (63). The insets in (C, D) depict the interfacing of peroxidase complex and membrane bilayer. The models for the protein-membrane complexes were built using the Orientation of Proteins in Membrane webserver (https://opm.phar.umich.edu/ppm_server) which calculates translational and rotational position of membranes and proteins from their three-dimensional structures. form carbon-centered radicals-the most important biological peroxidation catalysts are transition metals, particularly iron (Fe) (65). Therefore, the levels of redox active free Fe-ions or "loosely bound" Fe in low molecular weight complexes are strictly controlled in cells and biological fluids ( Figure 3A). Catalytic Fe of active enzymes is regulated by the protein structure. Fe required for these catalytic functions is delivered to the respective protein clients-Fe-sulfur proteins, hemoproteins, and nonheme Fe-proteins-by several specialized protein chaperons (66). Quantitatively, hemoproteins represent the most abundant endogenous source of Fe and catabolic degradation of these proteins accompanied by the release of ferrous ions (Fe 2+ ) is operated by heme oxygenases (67). Specialized ferroxidases convert (Fe 2+ ) to (Fe 3+ )-the form suitable for the intracellular iron storage by ferritin ( Figure 3). Normally regulated Fe-catalyzed reactions are enzymatic. However, under special conditions such as Fe-overload, excessive catabolism of Fe-proteins or diseases, aberrant nonenzymatic Fe-driven lipid peroxidation by poorly controlled low molecular Fe-complexes may occur, becoming excessive or even overwhelming (68). These reactions commonly proceed via the production of reactive oxygen species (ROS), particularly HO• radicals, generated in Fenton/Haber-Weiss reactions or via the formation of lipid radicals generated during decomposition of organic hydro peroxides, including lipid hydroperoxides (69) ( Figure 3B). As cell death programs are based on regulated mechanisms, the associated LPO is initiated by a selective and specific enzymatic process ( Figures 3C, D). Interestingly, the enzymes involved in the production of lipid death signals via peroxidation mechanisms during apoptosis and ferroptosis-cyt c and 15LOX-usually are involved in different biological functions. For example, cyt c is shuttling electrons between mitochondrial respiratory complexes III and IV in the intermembrane space (70). 15LOX is a dioxygenase catalyzing the formation of oxygenated lipid mediators from free PUFA, particularly free arachidonic acid [AA or eicosatetraenoic (ETE) acid 20:4] (71). Upon the initiation of the cell death program, these enzymes change their properties/functions and switch their activity to the peroxidation of PLs. The transformation of enzymatic activity occurs due to protein interaction with other molecules-a mitochondria-specific PL molecule, CL, in the case of cyt c ( Figure 2C), and the protein PEBP1 in the case of 15LOX ( Figure 2D).

Cyt c-Catalyzed Peroxidation of Cardiolipins
Cyt c is a small mitochondrial intermembrane space hemoprotein (MW about 12.5 kD, 104 amino acids) (72). As a transporter of electrons, cyt c utilizes hexa-coordinated heme whereby the Fe has four coordination bonds with a protoporphyrin IX and His 18 and Met 80 at the proximal and distal sides as the fifth and sixth iron ligands (73). Participation in the execution of the apoptotic death program is a recently established important function of cyt c. There are two pro-apoptotic processes that depend on cyt c: i) apoptosome formation and ii) CL peroxidation (16,74,75). These two seemingly unrelated roles of cyt c may, in fact, be closely linked to each other. Cyt c that is released from mitochondria into the cytosol interacts with the apoptotic protease-activating factor 1 (Apaf-1) to form the apoptosome, thus initiating the activation of caspase-9 and downstream caspases (75). The outer mitochondrial membrane (OMM) is permeable to small molecules-co-factors, small peptides, etc.-that get readily released through the pores with the size limit of~2nm in diameter (76). As the average diameter of the cyt c globule is~4nm, it is normally retained within the intermembrane space (77). It has been hypothesized that products of CL peroxidation may accumulate in the OMM where they can get oxidized and became involved in the production of pores with diameters >4nm that will facilitate the release of cyt c from the intermembrane space into the cytosol.
The accumulation of irreparable defects in mitochondria triggers their elimination through a special type of autophagy, mitophagy (78). This is a multistage process in which the signaling by a mitochondria-specific CL plays a prominent role. Normally confined almost exclusively to the matrix leaflet of the inner mitochondria membrane (IMM), CL undergoes several trans-membrane migrations to the mitochondrial surface (the outer leaflet of the OMM) (79,80). Externalized CL binds microtubule-associated proteins 1A/1B light chain 3B (LC3) (79), one of the central executioners of autophagy. Timely and successful elimination of damaged organelles via activation of mitophagy is a pro-survival mechanism (81). However, incomplete autophageal digestion of injured mitochondria with dysregulated electron transport represents a peril to the entire community of surrounding cells. As a result, the entire cell undergoes apoptotic elimination.
The transition from the pro-survival mitophagic to the apoptotic death program relies on a new interaction of cyt c with CL (which becomes possible during CL migration from IMM to OMM). Noteworthy, formation of the cyt c/CL complex causes a strong negative shift of its redox potential (by~400 mV) such that cyt c can no longer act as an electron acceptor from complex III, and function as an electron carrier in the respiratory chain (82). This results in the elevated production of superoxide anion-radicals and their dismutation to H 2 O 2 . The latter can be used as a source of oxidizing equivalents for a peroxidase reaction, provided that an inadvertent peroxidase activity is present in the microenvironment. The formation of cyt c/CL complexes offers this opportunity.
Evidently both pools of CL facing the intermembrane spacein the outer leaflet of the IMM and the inner leaflet of the OMM -bind cyt c to form a peroxidase complex activated by the available H 2 O 2 . Deprotonated CL phosphate groups can electrostatically interact with eight positively charged lysine residues of cyt c, particularly Lys 72/73 (74). The electrostatic binding is followed by strong hydrophobic interactions between PL acyl chains and non-polar regions of the protein (83). There are different views on the degree of the protein conformational changes induced by CL. While some of the data have been interpreted as the evidence for dramatic protein unfolding and denaturation ("molten globule"), recent multidimensional solid-state NMR results favors the model in which only minimal structural rearrangements take place whereby the hydrophilic milieu at the membrane interface stabilizes a native-like fold, but also leads to localized flexibility at the membrane-interacting protein face (84). One way or another, CL induced changes weaken and/or disrupt the coordination bond between heme-iron and Met 80 ( Figure 2C). Other amino acids can interact with the distal position of heme iron but they are not strong ligands and can be replaced by small molecules, including H 2 O 2 and FA-OOH (73). Overall, the catalytic site of the cyt c/CL complex adopts peroxidase activity triggered by the available H 2 O 2 or HOO-PUFA. If the iron in the cyt c/CL is in ferrous state, H 2 O 2 oxidizes it into ferric form thus completing the conversion of cyt c/CL into a peroxidase. H 2 O 2 leads to the formation of highly reactive oxoferryl porphyrin-pcationic radical (compound I), which can oxidize various substrates ( Figure 3C). In the case of pseudo-peroxidases, like cyt c/CL, compound I oxidizes protein amino acids to form a protein-immobilized radical (most likely tyrosyl) thus designating the emergence of compound II. Tyr 67 is located in closest proximity to the heme group of cyt c and mediates oxidation of CL ( Figure 2C) (85). The lower "pro-oxidant" capacity of compound II suggests that it is an unlikely candidate to further oxidize protein amino acids but it can readily abstract bis-allylic hydrogens from PUFA-CL (86). While other negatively charged lipids, like PIPs, PG, and PS, can also activate cyt c into a peroxidase, nevertheless the effectiveness of these alternative peroxidase complexes of cyt c with PLs is markedly lower than that of cyt c/CL complexes. Notably, the most abundant noncharged PLs of mitochondria, PC and PE, neither form peroxidase complexes with cyt c nor undergo peroxidation during apoptosis (16). Lipid hydroperoxides are orders of magnitude more effective in initiating CL peroxidation by cyt c/CL complexes than H 2 O 2 (87). This indicates that accumulation of small amounts of HOO-CL may strongly stimulate the peroxidation process. Indeed, progressive acceleration of CL peroxidation in the presence of CL-OOH has been experimentally confirmed (74). The described role and specific features of CL peroxidation by cyt c/CL complexes may inform the mechanism-based design of small molecule anti-apoptotic regulators with therapeutic potential as described below (88,89).

15LOX-Catalyzed Peroxidation of Phosphatidylethanolamines in Ferroptosis
The execution of ferroptosis includes the Fe-dependent production and accumulation of ox-PUFA-PL (27,90). Theoretically, both an enzymatic mechanism as well as a random free radical reaction may be engaged in this process. As sn2-15-HpETE-PE has been identified as a selective and specific product eliciting pro-ferroptotic activity, it is reasonable to assume that an enzymatic mechanism should be, at least in part, enacted in ferroptosis. Among several possible redox-catalyzing Feproteins, 15LOX has been proposed as the likely candidate (34). Mammalian LOXes are a family of non-heme iron containing dioxygenases that effectively catalyze oxidation of one or more 1,4cis,cis-pentadiene segments of free PUFA. A typical U-shaped PUFA binding channel is organized such that the oxidizable bisallylic carbon is juxtaposed to Fe. The LOX nomenclature-5LOX, 8LOX, 12LOX, 15LOX-is based on the ETE carbon position that is oxidized by the enzyme's Fe (91,92). The highly organized catalytic site contains Fe 3+ with five coordination bonds occupied by the protein's amino acids and the sixth position interacting with the hydroxide (in the native enzyme) or water (in the intermediate) ( Figure 3D). Fe 3+ -OH abstracts hydrogen from PUFA and yields a carbon-centered radical and Fe 2+ -OH 2 (93). Thus formed lipid radical (L•) interacts with molecular oxygen (O 2 ) delivered to the catalytic site through a special channel (94) which controls the production of the peroxyl radical at the catalytic site. The completion of the catalytic cycle is achieved via the hydrogen transfer from Fe 2+ -OH 2 to the peroxyl radical resulting in the formation of the lipid hydroperoxide.
Normally, 15LOX effectively oxidizes free ETE as a preferred substrate to generate 15-hydroperoxy-eicosatetraenoic acid (15HpETE) (71). Among the members of the LOX family, 15LOX is uniquely organized to also catalyze peroxidation of esterified PUFA, particularly membrane PLs (95). ETEphosphatidylethanolamines (ETE-PE) represent one of the preferred substrates for 15LOX leading to the production of 15-HpETE-PE (96). The catalytic efficiency of 15LOX toward ETE-PE is relatively low. However, its formation has dramatic consequences as 15-HpETE-PE has been identified as a proferroptotic signal (34). Paradoxically, a number of phenolic compounds and aromatic amines effectively prevent ferroptotic death but are poor 15LOX inhibitors (97). It has been hypothesized that 15LOX alone is not sufficient for the production of pro-ferroptotic death signals but there may be an additional factor modifying the enzymatic properties of 15LOX under ferroptotic conditions. Indeed, this factor has been identified as a scaffold protein, PE-binding protein-1 (PEBP1) (36). PEBP1 was shown to form a complex with 15LOX in which allosteric changes in 15LOX permit the entry and positioning of ETE-PE ( Figure 4) in a way that the enzymatic activity toward specific oxidation of the ETE-residue increases two-fold (99). Participation of 15LOX/PEBP1 in the generation of pro-ferroptotic PEox death signals was demonstrated in a number of different types of cultured and primary cells as well as in vivo in airway epithelial cells in asthma, kidney epithelial cells in renal failure, cortical and hippocampal neurons in brain trauma (100), and in intestinal epithelial cells after total body irradiation (101). Given the demonstrated necroinflammatory consequences of ferroptosis (102), the specific features of 15LOX/PEBP1 complexes offer an exciting opportunity for the design of ferroptosis-specific small molecule regulators with profound implications for human disease. It should be noted, however, that alternative enzymatic mechanisms of peroxidation of PUFA-PE may be involved in triggering ferroptosis. A recent study identified a NADPHdependent oxidoreductase (possibly with a partner isoform of cytochrome P450), as an essential activator of pro-ferroptotic phospholipid peroxidation in several types of cancer cells with low levels of 15LOX expression (103). The sensitivity of cells to pro-ferroptotic stimulation was decreased after downregulation of the oxidoreductase but not of 15LOX. In contrast, in cells with high levels of 15LOX expression (e.g., after stimulation of human airways epithelial cells by inducers of Th2 responses), 15LOX KD caused a strong suppression of ferroptosis (36).

Non-Enzymatic Lipid Peroxidation by "Loosely Bound" Fe-Complexes
Under extreme circumstances where the strict control of Fe is lost, Fe can display its redox activity mostly via participation in Fenton/Haber-Weiss reactions leading to the formation of ROS. The major biologically impactful event in these reactions is the reductive splitting of H 2 O 2 by Fe 2+ to yield highly reactive HO• radicals capable of initiating the oxidation process. H 2 O 2 reacts poorly with most biological molecules due the high activation energy barrier that must be overcome (104). The rate constant of the reaction of H 2 O 2 with free iron is low  (< 10 2 M −1 s −1 ), however, Fe-ligation may accelerate the reaction by several orders of magnitude (up to 10 4 M −1 s −1 ). In the context of lipid peroxidation, HO• radicals avidly attack lipid molecules to produce carbon-centered lipid radicals (69) which, in the presence of O 2 , are converted into peroxyl radicals (LO 2 •). The latter are much less reactive toward lipids and the abstraction of a hydrogen atom from the oxidizable lipid molecules represents the "most difficult" initiating event. The easiest "victims" of RO 2 • are bis-allylic positions in PUFA-hence their number in PUFA is the major factor defining the oxidizability of lipids. Random free radical chemical reactions are driven by the reactivity of the participating reagents-radicals and oxidation substrates and enzyme-imposed structural factors which do not limit the peroxidation process. Consequently, detection of selectivity of the peroxidation that deviates from this principle of oxidizability governed by the number of bis-allylic sites can be viewed as a strong argument against the participation of a free radical chemical reaction in the overall peroxidation process. Importantly, lipid peroxidation occurring during apoptosis and ferroptosis is highly specific not only with regards to classes of PLs-CL and PE. Indeed, linoleoyl (C18:2)-CLs represent the major substrates of pro-apoptotic peroxidation in mitochondria. Further, arachidonoyl (C20:4)-and adrenoyl (C22:4)-PE species, rather than more polyunsaturated docosapentaenoyl-and docosahexanoyl-PE species, are predominantly peroxidized in cells undergoing ferroptosis.
Another important difference between enzymatic and nonenzymatic LPO is that the latter is mostly driven by ferrous iron -in contrast to cyt c or 15LOX-dependent processes where ferric iron is the major catalytic species. In contrast, ferrous iron is markedly more effective in decomposing lipid hydroperoxides, the reaction leading to the production of secondary oxidatively truncated electrophilic products of LPO that can modify proteins and change their structure and functions. This is yet another controversy in understanding the leading role of Fe-dependent enzymatic vs. non-enzymatic reactions of LPO.

PRIMARY AND SECONDARY LIPID PEROXIDATION PRODUCTS
LPO-enzymatic or non-enzymatic-has a radical-mediated reaction in its nature. Radical intermediates are very short lived and cannot be directly detected by conventional high resolution analytical protocols such as LC-MS. The primary molecular products of LPO are hydroperoxides and they represent the first opportunity for the LC-MS based quantitative characterization. The analysis of lipid hydroperoxides has become a formidable task for several reasons. First, their chemical and metabolic instability and thermolabile nature most often results in the formation of secondary products, some of which are susceptible to degradation. Second, the sheer number of lipid signaling molecules is staggering which translates directly into a plethora of potential LPO products that, in many circumstances, occur at very low levels. Finally, while lipid hydroperoxides are the initial/ primary products of lipid oxidation, secondary products such as aldehyde, ketone, hydroxy, and epoxide products add to the heterogeneity and complexity of the signaling language. Thus, this rich signaling language should include not only full-length peroxidation products, but also truncated PLs, cyclized PLs as well as fragments of oxidized fatty acyl chains resulting from secondary reactions of lipid hydroperoxides (34,105).
Lipid hydroperoxides are present in very low steady-state concentrations and are unstable due to their cleavage to yield, dependent on the reducing or oxidizing environment, new alkoxyl-or peroxyl-radicals, which readily decompose into secondary products (34). Indeed, many studies have focused on small reactive lipid fragments, such as malondialdehyde, 4hydroxynonenal, etc. that can act as secondary downstream reactive products in a variety of cell death mechanisms and can covalently modify proteins [3]. However, this will depend on whether the reactive group (a reactive aldehyde for example) resides with the "leaving" short lipid fragment or remains with the truncated parent PL. Either of these two groups of reactive products can potentially react with nucleophilic amino acids, such as histidine, cysteine, and lysine that reside in cellular proteins. It has been well established that the process of PL peroxidation generates electrophilic species that are able to modify proteins and change their structure, activity and function (106). As PEs are preferentially peroxidized during ferroptotic death, it is likely that the truncated reactive parent PL is necessary for driving the downstream effects of ferroptosis ( Figure 5). Indeed, small reactive lipid fragments can be formed from any polyunsaturated (phospho)lipid class, hence will not be specific for ferroptosis. By forming conjugates with a protein, lipidation by truncated reactive parent PLs will undoubtedly change the hydrophobic-hydrophilic balance, likely changing the distribution of the proteins into membranes (107-109) where they can form dreadful oligomeric pores.
Peroxidation reactions catalyzed by cyt c/CL complexes yield a highly diversified set of oxidized CL species with hydroperoxy-, hydroxy-, epoxy-, and oxo-functionalities (110). These CL peroxidation products are similar to the CLox signals detected in cells during execution of the intrinsic mitochondria-mediated apoptotic program triggered in cells by actinomycin D (16), staurosporine (17), and ionizing radiation (22,111). Given that oxidizable PUFA-CLs are found exclusively in mitochondria, it is not surprising that oxidatively modified CL species, particularly mono-oxygenated C18:2-containing CLs, have been associated with the execution of the apoptotic death program (21). It should be noted, however, that a variety of CLox species, including hydroperoxy-, epoxy-, and oxo-CLs are detectable in cells and tissues in vitro and in vivo. For instance, CLox containing hydroxy-, epoxy-, oxo-, and hydroperoxy-functionalities have been detected in the small intestine of mice exposed to total body irradiation (110,112,113). Similarly, hydroxy-and hydroperoxy-CL species were detected in the brain of mice after traumatic injury (114). Notably, prevention of apoptosis by a mitochondrial electron acceptor, XJB-125, was associated with decreased levels of CLox in the peri-contusional zone of the traumatized brain.
It is possible that oxidatively truncated CLox species with high electrophilic potential can be formed in mitochondria. These products can readily attack nucleophilic sites in proteins and form protein adducts that will affect mitochondrial function and damage their integrity (115). Oxidatively truncated CL species can be formed in cyt c/CL catalyzed reactions in vitro ( Figure 6) and were detected in vivo in the ileum of mice exposed to total body irradiation (Figure 7). While it is possible that truncated CLox products can modify a number of mitochondrial proteins, including those involved in the execution of mitochondria-dependent cell death programs (e.g., apoptosis), this work has not been accomplished and represents a goal for future investigations. The isolation and determination of the site of lipidation on proteins directly participating in the execution of cell death in apoptosis and ferroptosis is a formidable task. However, lipidated proteins, and more importantly lipidated peptides generated from protein digests, should impart a dominant hydrophobic characteristic whereby the peptides should be highly retained on reverse-phase solid supports. Adjusting and modifying gradients for extremely hydrophobic species and/or applying different chromatographic solid supports should aid in the separation and identification of lipidated species.

INTRACELLULAR LOCALIZATION OF LIPID PEROXIDATION CENTERS Apoptosis
The execution of the intrinsic apoptotic program is initiated in mitochondria as it requires the oxidation of CLs (117). Formation of the peroxidase cyt c/CL complexes (16,118) along with the H 2 O 2producing interruption in electron transport causes accumulation of  CLox leading to the release of cyt c into the cytosol and apoptosome formation (75). In the cytosol, the released cyt c can bind an anionic phospholipid, phosphatidylserine (PS), located in the inner (cytosolic) leaflet of the plasma membrane. This complex also displays peroxidase activity that can trigger PS oxidation and accumulation of oxidized PS (PSox) (119,120). As a membranedisrupting agent, PSox can act as a "non-enzymatic scramblase" leading to the appearance of both PS and PSox molecules on the cell surface (120,121). Both lipids serve as universal "eat-me" signals in efferocytosis, whereby PSox is more efficiently recognized by professional phagocytes (122)(123)(124). Thus, two separate cyt cdependent oxidation mechanisms utilizing CL and PS are activated at different stages of apoptosis and generate signals with two distinctive functions. CLox is involved in the intracellular signaling at the initiation stage of apoptosis and leading to the release of cyt c from mitochondria, whereas PSox is an important part of inter-cellular communications regulating efferocytotic clearance of apoptotic cells by professional phagocytes (80,125).

Ferroptosis
Intracellular localization of LPO and participation of different organelles in the generation of ferroptotic death signal remains an important but still controversial issue (126,127). Fluorescence-based measurements using a reagent selectively A B FIGURE 7 | Peroxidized CLs, including oxidatively truncated species, are produced in the small intestine (ileum) of mice in vivo after total body irradiation (9.5 Gy) (110,116). Two truncated CL species, ONA/LA 3 -CL (A) and OA/LA 2 /ONA-CL (B), have been identified by MS 2 /MS 3 fragmentation analysis (ONA, 9-oxo-nonanoic acid; LA, linoleic acid; OA, oleic acid). As previously described (110,112,113,116), reacting with lipid hydroperoxides, Liperfluo, indicate that the endoplasmic reticulum (ER) is the major site of ferroptosis initiation (127). Both 15LOX and low molecular weight Fecomplexes are found in the ER compartment suggesting that both enzymatic initiation and non-enzymatic cleavage of HOO-PLs can take place in the ER (128,129). The ER can also promote ferroptotic peroxidation indirectly via induction of autophagy driving the degradation of several important regulators of lipid peroxidation such as ferritin, lipid droplets and glutathione peroxidase 4 (GPX4) (130,131). Given the likely engagement of mitochondria in the ferroptotic peroxidation, it is possible that mitochondria-associated ER membranes (MAMs) are the immediate locales where the LPO initiating events predominantly occur (132,133).
Mitochondria are likely to be directly and/or indirectly involved in pro-ferroptotic LPO. There are several lines of evidence favoring the mitochondrial participation: i) GPX4 is localized in the intermembrane space of mitochondria, whereas 15LOX is associated with the mitochondrial membrane (94,(134)(135)(136)(137), ii) a significant part (~40% of total phospholipids) of the major LPO substrate, PUFA-PE, is synthesized and present in this organelle (138), iii) they play a crucial role in cellular iron homeostasis, iv) they act as the major source of pro-oxidant ROS as well as a variety of molecular species of phospholipid hydroperoxides, v) they undergo dramatic morphological changes during ferroptosis (27). While strongly supportive, these characteristics make mitochondria a plausible but not penultimately proven universal participant of ferroptosis (139)(140)(141). It has been shown that cells incapable of generating mito-ROS due to depletion of mitochondrial DNA or with dramatically lowered levels of mitochondria (eliminated by mitophagy) did not demonstrate decreased sensitivity to ferroptosis (31). A highly effective mitochondria-targeted and hydrophobic radical scavenger, TPP-tagged MitoQ, was less effective than its non-targeted derivative (142). However, another mitochondria-targeted nitroxide, XJB-5-131, suppressed ferroptosis better than non-targeted nitroxides (143).
As an integral part of cell catabolism, lysosomes can, under some circumstances, indirectly participate in the ferroptotic program via autophagy e.g., by digesting Fe-containing proteins and releasing Fe used in pro-ferroptotic machinery (144,145). However, it appears that lysosomal LPO is not critical for ferroptosis, as prevention of the accumulation of ferroptosis inhibitor ferrostatin-1 (Fer-1) in lysosomes, caused a stronger inhibition of ferroptosis (31). Evidently, the plasma membrane may be a target for the ferroptotic program rather than a part of its execution machinery and phospholipid peroxidation products may participate in the late destructive stages of the cell's demise. In line with this, only minimal amounts of Fer-1 were detectable in the plasma membrane during execution of ferroptosis (146,147).

Non-Cell Autonomous Features of Lipid Signaling in Ferroptosis vs. Apoptosis
Preservation of plasma membrane integrity and formation of apoptotic bodies which are engulfed and removed by phagocytes are typical hallmarks of apoptosis. This process prevents spillover of cellular contents and makes apoptosis a non-inflammatory, cellautonomous phenomenon. Opposite to apoptosis, ferroptotic cell death is "spread," in a synchronized way, suggesting a direct cellto-cell communication for the delivery of death signals (38,(148)(149)(150). The nature of potential death signals was revealed by LC-MS based redox lipidomics in a number of experiments, including those with the induction of ferroptosis by exogenous pLoxA, and included the oxidation of exogenous ETE-PE yielding 15-HpETE-PEs. Results of these experiments demonstrated the synchronous character of the spreading of cell death. The fluorescence of Liperfluo interacting with 15-HpETE-PEs demonstrated propagation of these products among the neighboring cells suggesting that they can serve as a death signal initiating ferroptosis (38). The non-cell autonomous nature of ferroptosis may be associated with intercellular communications stimulating immune and metabolic responses (38).

REGULATION OF LIPID PEROXIDATION IN APOPTOSIS AND FERROPTOSIS AND ITS SPECIFICITY
The oxidation of CL during apoptosis occurs very early during the time when its molecular reactions leading to the initiation of apoptosis remain located in a restricted space inside of mitochondria whereby its inhibition provides an effective target for preventing apoptosis. This suggestion was confirmed by the experimental results demonstrating, that genetic depletion of CL-synthase as well as deficiency of cyt c or mutation of its Tyr 67 residue result in an increased resistance of cells to apoptosis (20,(151)(152)(153). Several low molecular weight compounds preventing CL oxidation by inhibiting of peroxidase activity of cyt c have been designed and tested, including mitochondria-targeted electron scavengers and stable nitroxides radicals (116,(154)(155)(156).
Regulation of LPO which play crucial roles in the development of ferroptosis is a key approach in preventing ferroptotic cell death. One of the most important defenders is glutathione peroxidase 4 (Gpx4), converting toxic PL hydroperoxides to non-toxic alcohols by using GSH as the reducing substrate (142). Direct inactivation of GPX4 by small molecular weight compounds interacting with selenocysteine such as RSL3, ML162, ML210, FINO 2 as well as indirect inhibition by deprivation of GSH were effective in inducing ferroptosis (28,126,157) (Table 1). One of the prerequisites for ferroptosis is the presence of ETE-PE, the substrate for the production of the death signal, HpETE-PE. Enzymes participating in the synthesis of ETE-PE such as ACSL4 responsible for the formation of CoA-derivatives of ETE and LPCAT3 catalyzing the esterification of CoA-ETE into lyso-PE, act upstream of GPX4 and are important ferroptosis regulators. Experimental data demonstrated a direct correlation between their expression and sensitivity to ferroptosis (34)(35)(36). GPX4 and ACSL4 double-KO cells have the ability to overcome the deadly effect of GPX4 deficiency and do not undergo ferroptosis (35). Some cells contain additional enzymatic mechanisms such as inducible nitric oxide synthase (iNOS)/NO• which can interact with 15LOX and/or lipid radicals and neutralize them (37). The anti-ferroptotic effect of NO• involves the inhibition of 15-LOX-dependent oxidation of ETE-PE and neutralization of HpETE-PE as well as secondary lipid radicals formed during cleavage and oxidative truncation of this molecule, thus preventing their toxic effects. Increased expression of iNOS/NO• in M1 macrophages/microglia or addition of NO• donors to the M2 macrophages not expressing iNOS lead to their high resistance to ferroptosis. Due to its ability to neutralize the formation of HpETE-PE, iNOS can inhibit ferroptotic cell death acting upstream of GPX4.
Nrf2/NFE2L2 transcription factor is activated as a feedback loop to protect cells from LPO generated during ferroptosis and it is one of the key players in the protection of cells from ferroptosis. After dissociation from its complex with its negative regulator Keap1, Nrf2 translocates to the nucleus where it activates the transcription of target genes (158,159). NRF2 inhibits ferroptosis through the regulation of hundreds of genes, including genes participating in the regulation of glutathione, GPX4 expression, iron, mitochondrial function and lipid metabolism (159,160).

Unique Role of Thiols
Reduced glutathione (GSH), the most prevalent non-protein thiol and the major intracellular antioxidant, plays an important role in maintaining a tight control over the redox status and cellular defense against LPO. However, the specific mechanisms of action of GSH in apoptosis and ferroptosis are different in spite of the fact that GSH depletion is a common feature of both extrinsic and intrinsic apoptosis (161). GSH depletion creates redox instability promoting the activation of signaling pathways leading to the initiation of apoptosis. One of the possible mechanisms activated by GSH depletion involves the deterioration of mitochondrial function. It appears that GSH deficiency promotes pro-apoptotic effects of other inducers; by itself GSH depletion is not sufficient to initiate apoptosis (162).
In ferroptosis, GSH is required as a substrate for proper functioning of GPX4. A decline in the GSH contents leads to the accumulation of pro-ferroptotic PUFA-PLox. The cystine/ glutamate antiporter (system xc − ) represents the main route for extracellular transport of cystine serving as an essential precursor for the synthesis of GSH. The xc − /GSH/GPX4 axis is the crucial controlling mechanism of ferroptosis and its inhibitors (e.g., erastin, imidazole ketone erastin, sulfasalazine, etc.) are classified as "class 1" ferroptosis-inducing compounds to distinguish them from RSL3 and other direct inhibitors of GPX4 classified as "class 2" (163).

Thiol-Independent Regulation of Lipid Signaling
A recently discovered protein FSP-1-formerly known as mitochondrial flavoprotein AIFM2-was found to inhibit ferroptosis in a GPX4/GSH-independent way. Accordingly, FSP1 knockout cells display a higher sensitivity to ferroptosis inducers whereas overexpression of FSP1 correlates with a higher resistance to ferroptosis in multiple tumor cell lines. The mechanisms of FSP-1 anti-ferroptotic effects involve its ability to serve as an oxidoreductase catalyzing the NAD(P)Hdependent reduction of CoQ to ubiquinol (43,44).
Similar to vitamin E, a lipophilic radical-trapping antioxidant CoQ10 is a very effective inhibitor of ferroptosis both in vitro and in vivo (164,165). The redox biochemistry of CoQ and vitamin E are intertwined: the more powerful antioxidant, vitamin E (tocopherol), neutralizes lipid radicals by donating an electron and can be regenerated back to its reduced form by CoQ-OH (166)(167)(168)(169). This recycling of vitamin E is maintained by FSP1 that maintains control of the reduced CoQ-OH. In addition to FSP-1, cells contain several oxidoreductases capable of reducing CoQ and vitamin E, hence be involved in the GSH/GPX4 independent inhibition of ferroptosis. Additional work in this area will elucidate the role of several oxidoreductases such as NAD(P)H: (quinone acceptor) oxidoreductase 1 (NQO1) (170), cytochrome b5 reductase (CyB5R), in CoQ/vitamin E anti-ferroptotic regulations (171).

MECHANISM-BASED REGULATORS OF ENZYMATIC PHOSPHOLIPID PEROXIDATION AS SPECIFIC ANTI-APOPTOTIC AND ANTI-FERROPTOTIC AGENTS
Contemporary understanding of the role, contribution and mechanisms of PL peroxidation as drivers of apoptotic and ferroptotic death programs, leads to the design of new classes of specific small molecule inhibitors with a potential for their application as therapeutic agents. To emphasize the importance of the structural and functional organization of the enzymatic complexes directly involved in the production of PL death signals, we will re-iterate their relevant and most important features in this section.

Anti-Apoptotic Regulators
Oxidation of mitochondrial CL by the cyt c/CL peroxidase complex is an early and critical step in apoptosis signaling. CL oxidation facilitates the release of cyt c from mitochondria to the cytosol and participates in apoptosome formation. CL depletion disrupts the function of various IMM enzymatic complexes (80,172). Regulating enzymatic CL oxidation is a promising LPO-focused anti-apoptotic therapeutic strategy. CL is mitochondria-specific and asymmetrically localized, being overwhelmingly localized and enriched (≤25% of total PL) at the IMM inner leaflet. To interact with intermembrane cyt c, CL is first flipped to the IMM outer leaflet. The mechanisms of the overall process leading to CL externalization remain enigmatic. Several candidate proteins contribute to loss of CL asymmetry following injury-such as PL scramblase-3 (PLS3) that facilitates transposition of CL from the inner-to-outer IMM leaflets and other transporters implicated in IMM-to-OMM CL transport [e.g., mitochondrial nucleoside diphosphate kinase (NDPKD), adenine nucleotide translocator (ANT), uncoupling proteins (uCP), creatine phosphokinase (CPK), and truncated-BH3 interacting domain death agonist (t-Bid)] (173,174).
Interaction between CL and cyt c is mediated by electrostatic forces between positively charged lysines in cyt c and CL's two negative phosphate groups (74,175). Binding of CL induces: 1) distortion of cyt c's heme-associated Trp 59 and reduction in cyt c's hydrophobic core volume; 2) disruption of cyt c's Met 80 -Fe bond, lead to a mixture of penta-coordinated and His 33 /His 26 hexa-coordinated heme (176); 3) a decreased Fe(II)/Fe(III) couple redox potential (~400 mV more negative than native cyt c) abolishing its electron shuttling activity while gaining peroxidase activity (177,178); and 4) opening of cyt c's heme crevice enabling substrate access to the newly formed peroxidase active site (153,179). The newly formed cyt c/CL peroxidase substrate specificity leans toward organic peroxides, like CLox. However, given the complex's proximity to mitochondrial H 2 O 2 sources (ETC complexes and TCA dehydrogenase) and electrostatic affinity for unoxidized CL, the primary substrate of the cyt c/CL complex is CLox (180,181). Liberation of CL's oxidized acyl chains through the action of phospholipases (e.g., iPLA 2 g) yields a suite of immune activating signaling molecules-such as hydroxyoctadecadienoic acids (HODEs), hydroperoxyoctadecadienoic acids (HpODEs), and hydroxy-eicosatetraenoic acids (HETEs) (182,183).
Small molecule therapies targeting CL peroxidation hold promise as a specific anti-apoptotic interventions. However, the administration of non-targeted global antioxidants (e.g., vitamin E, CoQ10, or nitroxyl radicals) proved ineffective at improving survival and CLox-associated apoptosis in acute injury models. Poor localization of the therapeutic compounds to the pathogenic target sites, low activity with the target substrate, and/or deleterious off-target effects on ROS signaling may have led to this failure (184). These limitations may all be overcome, to varying degrees, by specifically targeting and enriching antioxidant and electron scavengers, like nitroxide, to the IMM. XJB-5-131 and JP4-039 are comprised of a hemigramicidin (HS) peptide conjugated to TEMPO (114). These compounds attenuate lesion volume and improve behavioral deficits following experimental brain injury. Mechanistically, XJB-5-131 serves as an electron scavengers, preventing O ·− 2 and H 2 O 2 formation and limiting fuel for the cyt c/CL peroxidase. Alternatively, mitochondrial-targeted imidazole-conjugated fatty acids (TPP-ISA, TPP-IOA) coordinate the hepta-coordinated form of the cyt c/CL complex, impeding CL access and oxidation ( Figure 8) (80). Therapies may also promote CL lipidome remodeling into a less oxidizable, mono-unsaturated form using mitochondria targeted oleic or stearic acid derivatives (185). While perhaps less viable for application in acute injury, this remodeling approach has shown potential in chronic neurodegenerative disease models (114). Development of new mitochondriatargeted molecules that suppress CL peroxidation, as well as the optimization of the pharmacodynamic/pharmacokinetic properties of existing ones may lead to promising ant-apoptotic therapeutic modalities. FIGURE 8 | Radiation protection and mitigation by TPP-IOA and TPP-ISA. C57BL/6NTac female mice were exposed to total body irradiation to a dose of 9.25 Gy using a cesium source (n = 31-35 mice per group). The mice were irradiated and injected i.p. with TPP-IOA or TPP-ISA (5 mg per kg body weight in 100 ml of water containing 25% ethanol) 10 min after irradiation. P < 0.0001 (a two-sided log-rank test)-TPP-IOA or TPP-ISA injected and exposed to total body irradiation mice vs. mice exposed to total body irradiation only. These figure was adapted from (116).

Anti-Ferroptotic Regulators
Most anti-ferroptosis strategies target LPO in one way or another -whether they promote cellular lipidome remodeling into a less oxidizable form, inhibit LOXs, or serve as lipophilic radical trapping agents. As indicated above, many of the ferroptotic inhibitors exhibit both LOX inhibition and radical trapping capacity, although to varying degrees (186,187). The lack of appropriate analytical tools hindered the understanding of the precise mechanism of these molecules. With regards to the LOX inhibitors, their anti-ferroptotic effectiveness is often higher than the LOX inhibitory activity (97). Notably, the EC 50 values for LOX inhibition have been calculated exclusively based on the suppression of free HpETE production by LOX alone. The rate of the HpETE production by 15LOX is many-fold higher than that of HpETE-PE. Because the latter, but not free HpETE, act as proferroptotic signals, the meaning of these estimates in the context of ferroptosis is dubious (34). Assuming that the 15LOX/PEBP1 complex is the generator of pro-ferroptotic HpETE-PE signals (36,188), assessments of small molecule radical scavengers have to be performed in this system. A recent study conducted with Fer-1, known to act as good radical scavenger, demonstrated that the EC 50 value for Fer-1 in inhibiting the HpETE-PE production by the 15LOX/PEBP1 complex is considerably lower than its effective cytoprotective concentration in cells triggered to undergo ferroptosis (98). Moreover, the Fer-1-driven ferroptosis suppression was mainly realized through its enzymatic inhibitory capacity (98). It should be noted that the radical trapping reagents could have off-target effects that may decrease the (phospho)lipid peroxide load of the cellular system. These effects may be helpful to mitigate diseases involving multimodal mechanisms of cell death as exemplified by traumatic brain injury and inflammatory disease triggered by total body gammairradiation (36,46,189). At the same time, highly specific inhibitors could be beneficial for diseases in which ferroptosis is the predominant pathogenic mechanism.

CONCLUDING REMARKS
PLs are the major building blocks of the membrane bilayer and their structural role is indispensable and fundamental to compartmentalization and interfacing of thousands of processes in cells and their organelles. In this capacity, macroscopic characteristics of the PL assembles such as fluidity, flexibility, lateral and trans-membrane diffusion are essential for the function of membranes as interactive barriers. Interconnected with this is the signaling by membrane phospholipids that is associated with their post-synthetic modifications. Most commonly this occurs via oxidative transformations of polyunsaturated PL resulting in the production of a huge variety of new molecular species of lipids, integrated into a new concept of the epilipidome. The epilipidome includes not only oxidatively modified lipids but also a huge variety of their adducts with proteins thus merging with the epiproteome, post-translationally modified proteins. Signaling by individual lipids and lipoprotein adducts comprising the epilipidome/epiproteome is broadly employed in almost each of the myriads of life-defining activities of cells and cell communities. This type of signaling is also essential for coordination of regulated cell death programs. Although these programs may be viewed as suicidal, their strict control and harmonized execution defines the borderline between health and disease. Therefore, deciphering the signaling phospholipid language of these programs is one of the central areas of research not only in cell biology but also in many fields of biomedicine. While lipid peroxidation has been attributed to essentially all known death programs, its specific mechanisms and role have been clearly defined for only two of them-apoptosis and ferroptosis. Formation of free radical intermediates in the course of LPO occurs both during regulated enzymatic and random non-enzymatic processes. As a result of this, a broad spectrum of agents of different classes with high hydrogen/ electron-donating capacities, particularly phenolic compounds and aromatic amines, may be effective in blocking the LPO component of death programs. This has created an optimistic view that many of these compounds may lead to "anti-suicidal" cell-based therapies. The indiscriminative nature of this strategy -likely affecting multiple vital biochemical reactions proceeding via free radical intermediates-may be associated with low effectiveness and serious side effects. Free radical scavengers/ sacrificial antioxidants/chain-breaking antioxidants-have been designed and developed and ultimately tested in numerous clinical trials in more than three dozens of diseases. Disappointingly, the results of these were uniformly negative. This strategic mistake in searches for a "magic antioxidant bullet" should be avoided in the design of new anti-apoptotic and anti-ferroptotic therapeutic agents-the lesson has to be learnt. Future generations of small molecule regulators of regulated cell death program therapies must consider their selective and specific mechanisms, hence, to be precise and highly discriminative. In line with this, distinctive inhibitors/ regulators have to be developed for controlling individual cell death programs-as has been discussed above.