LPS-Induced Systemic Inflammation Caused mPOA-FSH/LH Disturbance and Impaired Testicular Function

Male reproductive function is key to the continuation of species and is under sophisticated regulation, challenged by various stressors including inflammation. In the lipopolysaccharide (LPS) intraperitoneal injection-induced acute systemic inflammation, male fecundity was compromised with decreased testosterone level, damaged spermatogenesis, and downregulations of testicular gene expression levels involved in steroidogenesis regulation and blood–testis barrier. It is also noteworthy that the testis is more sensitive to acute stress caused by LPS-induced systemic inflammation. LPS treatment resulted in lower testicular gene expression levels of steroidogenic acute regulatory protein, cholesterol side-chain cleavage enzyme, and cytochrome P450 family 11 subfamily B member 1 after LPS treatment, while no such decrease was found in the adrenal gland. In parallel to the significant decreases in testicular intercellular adhesion molecule 1, tight junction protein 1, and gap junction alpha-1 protein gene expression with LPS treatment, no decrease was found in the epididymis. In the brain, LPS treatment caused higher medial preoptic area (mPOA) activation in the hypothalamus, which is accompanied by elevated blood follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels, suggesting a disturbed hypothalamic–pituitary–gonad axis function. Besides mPOA, brain c-fos mapping and quantitative analysis demonstrated a broad activation of brain nuclei by LPS, including the anterior cingulate cortex, lateral septum, paraventricular nucleus of the hypothalamus, basolateral amygdala, ventral tegmental area, lateral habenular nucleus, locus coeruleus, Barrington’s nucleus, and the nucleus of the solitary tract, accompanied by abnormal animal behavior. Our data showed that LPS-induced inflammation caused not only local testicular damage but also a systemic disturbance at the brain–testis axis level.


INTRODUCTION
Male infertility affects about half of couples who cannot conceive (1). The main causes of male infertility can be generally divided into three categories: hypogonadism due to hypothalamicpituitary abnormity, seminal outflow disability, and testicular dysfunction, while the last one makes up the most common cause of male infertility (1)(2)(3). Many kinds of stressors impact normal testes functions, including heat, oxidative stress, environmental contamination, and psychological stress (4)(5)(6). Many studies have focused on the cellular and molecular pathways of these stressors on the testes, although the application of most stressors was not restricted to the testes alone and did not solely exert their influence locally at the testes, such as persistent organic pollutants (7,8) and systemic inflammation (9,10). Testicular dysfunctions were noticed in plenty of pathological states of systemic inflammation (10)(11)(12), while much less attention was paid to the contribution of systemic inflammation to testicular dysfunctions. On the other hand, while the testicular function is under sophisticated regulation of the central nervous system (CNS) through the classical hypothalamic-pituitary-gonadal (HPG) axis, most studies have focused on testicular function regulation at the peripheral organ level (13); there is limited evidence of possible CNS participation in stress-induced testes dysfunction, which is perhaps surprising considering the well-established role of HPG axis in regulating testicular functions.
Clinically, close relationships between male reproductive diseases such as male infertility (14,15), orchialgia (16), and psychological stress, anxiety, or depression have been reported. A study of patients undergoing in vitro fertilization showed that both state and trait anxiety had a negative impact on ejection volume, sperm count, and sperm motility (17). The negative association between semen quality and perceived stress has also been observed (18,19). Infertile men reported a higher number of stressful life events compared to a fertile group (20). In addition, psychological stress such as earthquake experience was shown to significantly impair the semen quality of survivors even after many years, demonstrating long-term effects (21,22). Despite the reported association between testisrelated diseases and psychological disorders, evidence addressing the underlying mechanisms of the systemic regulation at both the central and peripheral levels in animal models is scarce.
Lipopolysaccharide (LPS) was reported to induce brain inflammation and disturbance of the blood-testis barrier (BTB) as well (23,24). In this study, we applied LPS, which is an important constituent of the outer membrane of Gramnegative bacteria to induce orchitis (25) and systemic inflammation as an acute stressor. Here, we proposed the hypothesis that CNS is involved in the progress of testicular dysfunction at acute systemic inflammation stress. The impact of LPS administration on testosterone production, the integrity of BTB, spermatogenesis, brain nuclei activation, and corresponding behavior outputs were studied. Our study offers an approach to study possible CNS involvement in acute stress-induced testicular impairment.

Animals and LPS treatment
Adult (6-8 weeks) C57BL/6J male mice (Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China) were housed in groups of five, had ad libitum access to food and water and were maintained on a 12:12-h light/dark cycle (lights on from 8:00 a.m. to 8:00 p.m.). Mice were given a single dose of 4 mg/kg lipopolysaccharides (LPS, L2630, Sigma, Saint Louis, Missouri, USA) or same volume of saline via intraperitoneal (i.p.) injections. All husbandry and experimental procedures in this study were approved by the Animal Care and Use Committees of the Shenzhen Institute of Advanced Technology (SIAT), Chinese Academy of Sciences (CAS), China.

Radio-Immunoassay Assay of Testosterone
Mice plasma samples were collected at the afternoon of the experimental day. Plasma testosterone levels were measured using an Iodine[ 125 I] Radio-Immunoassay Assay (KIP1709, DIasource, Louvain-la-Neuve, Belgium). Blood samples from the LPS-treated and control mice were collected with ethylenediaminetetraacetic acid (EDTA)-pretreated centrifuge tubes and gently mixed at room temperature before centrifugation to prevent blood coagulation. Supernatant plasma was collected and stored at −80°C prior to use. Standard testosterone samples were used to prepare a standard curve. Both standard testosterone samples and plasma samples were incubated with rabbit anti-testosterone antibody at 37°C for 1 h. Then, donkey anti-rabbit separating agent was added, and the mixture was kept still at room temperature for another 15 min prior to centrifugation at 3,500×g for 10 min. The absorbance was normalized to standard testosterone to calculate each sample (Beijing North Institute of Biotechnology Co., Ltd., Beijing, China).

MILLIPLEX ® Multiplex Assays Using Luminex ® Testing Plasma Cytokine Concentration
Plasma samples were analyzed with Endocrine Multiplex Assay (MADKMAG-71K, Merck Millipore, Burlington, Massachusetts, USA) to determine levels of interleukin 6 (IL6), tumor necrosis factor alpha (TNFa), monocyte chemoattractant protein 1 (MCP1), according to the manufacturer's protocol. Briefly, after incubation of the assay buffer for 10 min at room temperature, experimental plasma samples or gradient dilution standard samples were added to the wells of a 96-well plate and supplemented with assay buffer, serum matrix, and then neat samples. Dyed antibody-bound beads were then added and shaken gently before being left to incubate overnight at 4°C. Next, detection antibodies were added to each well following a washing step and incubated with agitation for 30 min at room temperature. Streptavidin-phycoerythrin was added to the plate and was incubated for another 30 min. The plate was washed for three times, and sheath fluid was added to resuspend the beads for 5 min. Fluorescent intensity quantification was performed using a Luminex analyzer (Luminex 200, Merck Millipore, Burlington, Massachusetts, USA). Data were analyzed using MILLIPLEX software (Analyst.V5.1, Merck Millipore, Burlington, Massachusetts, USA).

Enzyme-Linked Immunosorbent Assay of Luteinizing Hormone and Follicle-Stimulating Hormone
Plasma luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured using ELISA kit (EM1188 and EM1035, FineTest, Wuhan, China) according to the manufacturer's instructions. Briefly, experimental plasma samples and gradient dilution standard samples were incubated with biotin-labeled antibody in the pre-coated plate, respectively, for 45 min at 37°C. The plates were washed with wash buffer for three times; then, the horseradish peroxidase -streptavidin conjugate was added to the plates and incubated for 30 min at 37°C. The plates were washed five times with wash buffer; TMB substrate was added and incubated at 37°C in dark for 15 min. When the standard wells showed apparent gradient, stop solution was added to each well and subjected to a 450 nm in a microplate reader (Synergy H1, Bio-Tek, Santa Clara, California, USA) immediately. The absorbance was normalized to standard LH and FSH, respectively, to calculate the value for each sample.

Hematoxylin and Eosin Histology and c-fos Immunofluorescence Staining
Forty-eight hours after LPS injection, mice were put under deep anesthesia and transcardially perfused 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS), using saline-injected mice as controls. Histological analysis of H&E and immunofluorescence staining was performed as previously reported (26). For H&E staining, fixed testes and epididymis were harvested and postfixed with 4% PFA for 24 h, then embedded in paraffin, sliced into 4-mm sections with a microtome (RM2016, Lecia, Wetzlar, Germany), and subjected to deparaffinization, hydration, and then stained with hematoxylin and eosin (AS1055A and AS1094, Aspen, Wuhan, China). For c-fos mapping, the brains were harvested, postfixed with 4% PFA, and cryoprotected with 30% sucrose in PBS. For immunofluorescence staining, mouse brains were dehydrated in 30% sucrose and then sliced into 30-µm sections using cryostat (CM1860, Lecia, Wetzlar, Germany). Brain sections were stored at −20°C with a cryoprotectant prior to histological analyses. Tissue sections were first washed with PBS to remove embedding medium; antibody staining was performed in 24-well plates on floating tissue sections. Sections were blocked with normal goat serum for 1 h at room temperature and then incubated overnight in primary antibodies at 4°C, followed by PBS washing and incubation with secondary antibodies at room temperature for 2 h. The primary antibodies used in this study were rabbit anti-c-fos (2250, Cell Signaling Technology, Danvers, Massachusetts,USA, 1:500). Alexa Fluor@488-conjugated AffiniPure fab fragment goat anti-rabbit secondary antibody (111-547-003, Jackson ImmunoResearch, Philadelphia, Pennsylvania, USA, 1:200) was used. Sections were then incubated with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, D9542, Sigma, Saint Louis, Missouri, USA, 0.4 mg/ml) for 5 min. Confocal images were taken with a confocal microscope (LSM 880, Zeiss, Oberkochen, Germany) and scanning images were taken with a virtual microscopy slide scanning system (VS120, Olympus, Tokyo, Japan).

RNA Extraction and Real-Time Quantitative PCR
To explore expression levels of blood-testis-barrier-related, steroidgenesis and inflammatory genes, testicular tissues, epididymis tissues, adrenal gland and hypothalamus tissues from LPS-treated and control animals were collected and digested to perform real-time quantitative PCR analysis. Total RNA was extracted from the testis, epididymis tissue or hypothalamus tissue with Trizol reagent (15596018, Invitrogen, Carlsbad, California, USA) and quantified using a micro-spectrophotometer (NanoDrop One, Thermo Scientific, Massachusetts, USA). One microgram of total RNA was used for cDNA synthesis using ReverTra Ace qPCR RT Kit (FSQ-101, TOYOBO, Osaka, Japan). Real-time quantitative PCR was performed using SYBR ® Green Real-Time PCR Master Mix (QPK-201, TOYOBO, Osaka, Japan), with a LightCycler ® 480 Instrument (Roche, Basel, Switzerland). b-Actin was used to normalize expression levels of the genes tested. The primers used in this study are shown in Table 1.

Animal Behavior Test and Analysis
An open-field test (OFT) was used to assess anxiety-like behavior and locomotor activity in an open-field arena (50 cm × 50 cm × 50 cm). For analysis, the center of the arena floor covering 25% of

Transcripts
Forward primer Reverse primer ATGGCATGGCTTACACCACC GAGGCCAATTTTGTCTCCACA the total floor area was defined as the center area (i.e., 25 cm × 25 cm). The OFT was used to assess behavior following LPS stress. Mice were first allowed to freely explore the open field for 1-2 min prior to a 10-min observation session. The number of entries to the center area and time spent in the center during the 10-min session were recorded and analyzed using Anymaze software (Stoelting, Chicago, Illinois, USA

Statistical Analysis
All data in this study were analyzed as mean ± SEM. Statistical significance was calculated using GraphPad Prism (GraphPad Software, San Diego, California, USA) and labeled as ns, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Student's t-test or Kruskal-Wallis with Dunn post-hoc test was used as appropriate. All n values in this study refer to the number of mice used in each experiment, as shown in each figure legend.

LPS Treatment Resulted in Elevated Circulating Inflammatory Cytokines
Adult male C57BL/6J mice were intraperitoneally injected with a single dose of either 4 mg/kg LPS in 0.9% saline or with 0.9% saline only. After 48 h, bodyweight decrease in the LPS group was significantly larger than that of the control group (p < 0.0001, Figure 1A). There were higher plasma levels of inflammatory cytokines such as TNFa (p < 0.001), IL6 (p < 0.01), and MCP1 (p < 0.001) in the LPS group compared to the controls ( Figures 1B-D), gene expression analysis of testis also revealed local testicular inflammation (Supplementary Figure 1). In summary, acute stress caused by LPS administration resulted in a significant physiological disturbance in terms of inflammation.

LPS Treatment Impaired Spermatogenesis and Decreased Gene Expression of Blood-Testis-Barrier-Related Proteins
H&E staining revealed immature male gametes in the lumen of seminiferous tubules of the testis ( Figure 2A) and in the lumen of the epididymis ( Figure 2B) 48 h after LPS treatment. Quantification of the gametes in the epididymis showed a statistical difference between the LPS treatment group and the saline control group (p < 0.05, Figure 2C). The BTB lays the structural foundation of the spermatogenesis niche (27). Realtime quantitative PCR analysis of the testicular tissue revealed lower expression levels of genes coding for BTB-related proteins, including intercellular adhesion molecule 1 (Icam-1, p < 0.05), tight junction protein 1 (Tjp1, p < 0.05), and gap junction alpha-1 protein (Gja1, p < 0.05) in the LPS-treated groups compared to controls ( Figure 2D). Using the epididymis tissue as a control, we found no such difference in gene expression levels of Icam-1, Tjp1, or Gja1 in the LPS-treated epididymis, indicating that LPS administration led to a dysfunction of cell-cell interaction in testes, rather than the epididymis ( Figure 2E).

The LPS-Treated Group Had Lower Plasma Testosterone Levels and Suppressed Gene Expression of Key Steroidogenesis Enzymes
Radioimmune assay (RIA) showed that LPS-treated group had lower plasma testosterone levels than the controls (p < 0.05, Figure 3A). We then performed real-time quantitative PCR to evaluate gene expression levels of key steroidogenesis enzymes using another batch of mice. Reduction in steroidogenic acute regulatory protein (StAR, p < 0.05), cholesterol side-chain cleavage enzyme (P450scc, p < 0.001), 3b-hydroxysteroid dehydrogenase (3b-HSD, p < 0.01), and cytochrome P450 family 11 subfamily B member 1 (Cyp11b1, p < 0.05) was  found in the LPS treatment group compared to controls ( Figure 3B). In the adrenal glands, another primary steroidogenesis organ, only one steroidogenic gene had reduced expression (3b-HSD, p < 0.01), whereas expression of the other three genes were not different between groups (StAR, p = 0.8129; P450scc, p = 0.1979; Cyp11b1, p = 0.1740; Figure 3C). These results suggest that LPS treatment caused an impairment in male sex hormone production. The testes seem to be more vulnerable to the LPS-caused stress than the adrenal glands in terms of steroidogenic gene expression levels.

LPS Treatment Activated the Medial Preoptic Area in the Hypothalamus and Increased Circulating Follicle-Stimulating Hormone and Luteinizing Hormone
We observed abnormal behaviors after LPS treatment. When subjected to OFT, LPS-treated mice showed decreases in total distance travelled, entries to center, and time in center ( Supplementary Figures 2A, B). In the EPM test, LPS treatment also induced decrease in total distance travelled and a trend in Entries to Open arms and Time in Open arms (Supplementary  Figures 2E-G). The abnormal behavior caused by LPS treatment suggested neurotoxicity of LPS as reported before. Gene expression analysis of hypothalamic tissues revealed elevated levels of inflammatory cytokines (Supplementary Figure 3). Considering the importance of the HPG axis in regulating testicular function (28), we wanted to know whether LPS treatment induced any change in neuronal activity in the hypothalamus, especially the medial preoptic area (mPOA) where most the gonadotropin-releasing hormone (GnRH) neurons are located. Immunostaining of c-fos early response gene as an indicator of neuronal activation showed significant increase in the mPOA of the LPS group ( Figures 4A, B p < 0.05). ELISA results showed changes in circulating FSH (p < 0.05) and LH (p < 0.001) levels after LPS treatment ( Figures 4C, D), suggesting LPStreatment resulted in disturbed HPG-axis functions ( Figure 4E).

LPS-Treatment Resulted in Vast Brain Activation
Besides mPOA, c-fos immunofluorescent staining showed broad activation of brain nuclei in the LPS-treatment group than controls, including nuclei of the forebrain, thalamus, hypothalamus, brainstem, and cortex. Cell counting and analysis showed that there was significantly higher number of c-fos-positive cells in the LPS group compared to the saline control group in the following brain nuclei: the anterior cingulate cortex (ACC), lateral septum (LS), basolateral amygdala (BLA), paraventricular nucleus of the hypothalamus (PVN), lateral habenular nucleus (LHb), ventral tegmental area (VTA), locus coeruleus (LC), Barrington's nucleus (BAR), and the nucleus of the solitary tract (NTS) (Figures 5A, B). Interestingly, most of the LPS-activated brain nuclei that we observed are participants in anxiety circuits and involved in stress responses (29), which was in line with the behavioral outputs that we observed (Supplementary Figure 2E-G).

DISCUSSION
Our study demonstrates that testis impairment, including a reduction in testosterone level and impaired spermatogenesis, can be induced by acute LPS stress. These changes were accompanied by a significant reduction in BTB-related gene expression, decreased steroidogenesis regulating protein gene expression, activation of mPOA, disturbed HPG-axis function, activation of stress-related brain nuclei, and, furthermore, anxiety-like behavior. LPS intraperitoneal (i.p.) injection had been used to induce atypical orchitis model in rodents (30,31), and we observed elevated testicular gene expression of inflammatory cytokines (Supplementary Figure 1). LPS-induced systemic inflammation resulted in damaged spermatogenesis and testicular steroidogenesis. Yet in the previous reports, the underlying mechanism was focused on the local testicular mechanism. As a matter of fact, LPS injection caused a systemic inflammation in the whole body instead of local testicular inflammation, as we found that LPS indeed induced a systemic inflammation with elevated levels of IL6, TNFa, and MCP1. Importantly, in clinical reports, systemic inflammation is more common than inflammation that happens only inside the testis (10)(11)(12). It would be intriguing to find that LPS-caused systemic inflammation would affect testicular function through the hypothalamus-pituitary-gonad axis.
Leydig and Sertoli cells are very important for testicular functions, and they have been intensively studied by previous works using in vivo and in vitro experiments with LPS exposure (32)(33)(34). In our study, we accessed the testosterone levels between the control and LPS-treated groups, as a direct indicator of Leydig cell function. Furthermore, in line with a previous report (32), LPS treatment caused a decrease in plasma testosterone levels, which was accompanied by decreased gene expression levels of steroidogenesis-regulating enzymes including StAR, P450scc, 3b-HSD, and Cyp11b1. It is interesting to notice that, using adrenal gland as a control organ, gene expression levels of P450scc, 3b-HSD, and StAR in the adrenal gland were not so badly impaired as in the testes. The testes seem to be more vulnerable to the acute stress caused by LPS injection than the adrenal glands in terms of steroidogenic gene expression levels.
Besides the damaged hormone-producing function of testis, our results also showed that LPS injection caused impaired spermatogenesis, as demonstrated by immature gametes in the lumen of testicular tubules and epididymis lumen. This impaired spermatogenesis might be caused by disturbed BTB as previously proposed (27,35,36), as weakened BTB negatively affects different stages of spermatogenesis (37). For example, Icam-1 downregulation is involved in BTB disturbance and spermatogenesis impairment in busulfan-treated mice (38). The junctions between Sertoli cells of the seminiferous tubules, near the base membrane (39), are the most important component of BTB. Sertoli cells were reported to have high expression levels of Icam-1, Tjp1, and Gja1 (40,41). BTB-related ZO-1/TJP1 protein level can be downregulated by heat stress (42); direct TNFa administration reduces expression of the Tjp1 in the testes and disrupts the BTB (43). Homozygous knockout of Gja1, another BTB gene that codes the gap junction protein connexin 43, leads to infertility in mice (44). In our study, we found a significant decrease in gene expression levels of Icam-1, Tjp1 or Gja1 in the testis, while no such decrease in BTB-related gene expression was observed in the epididymis. In another word, in LPS-induced epididymitis, BTB-related gene expression was not as badly affected as in the LPS-induced orchitis, indicating a different regulatory mechanism under stress between the two reproductive organs, which again suggests the vulnerability of the testes as a vital reproductive organ. Furthermore, LPS administration has been reported to induce brain inflammation (45,46), impair neurogenesis and memory (47), and even cause sporadic Alzheimer's disease (48). The receptor of LPS, TLR4, was reported to be induced in the brain by LPS treatment. There was elevated expression of TLR4 in both glia and neuron, with wide expression in microglia (49), astrocyte (50), oligodendrocyte (49), and neuron (51). A previous report showed that LPS injection induced a significant increase in LH at about 2.5 days after LPS treatment (52). Another study indicated a time-dependent increase in LH and FSH after LPS engagement (53). Considering the important regulations of the HPG axis on testicular functions, we studied the mPOA of the hypothalamus, where most GnRH neurons are located, and is key to the HPG-axis regulation. Our results showed an activation of the mPOA brain nucleus by LPS injection, together with elevated circulating levels of LH and FSH. Hence, LPS treatment induced a stressed state not only in the testes but a broader disturbance including the brain. We speculated that this elevated LH and FSH levels observed in our study could be a direct result from mPOA activation. It could also be the secondary response of disinhibition of the HPG axis following the decreased testosterone production from the testis. Lower testosterone due to the impairment of steroidogenesis led to subsequent weakened negative feedback on the CNS. This disturbance of HPG-axis function might in turn contribute to the dampened steroidogenesis, damaged spermatogenesis, and perturbance of BTB, suggesting that LPS-caused acute inflammation not only disturbed testicular function through local testicular mechanism but also through multilevel of HPG axis to impact testicular function.
Interestingly, mPOA was not the only brain nucleus activated by LPS injection; broad brain nuclei including ACC, LS, BLA, PVN, LHb, VTA, LC, BAR, and NTS, were found to be activated, too. Most of the above nuclei are reported to be upstream of mPOA with monosynaptic connection to mPOA (54)(55)(56). It has been long known that VTA innervates mPOA through dopaminergic projections (54). Retrograde tracing revealed direct projection from LS, PVN, VTA, LC, amygdala (55), and NTS (56) to mPOA. Among these activated nuclei, the PVN is one of the most interesting, since it plays an important role in stress response through the hypothalamic-pituitary-adrenal (HPA) axis (57) and has direct neural connections to mPOA (58). Another interesting nucleus is the brainstem LC, which is the main source of the brain noradrenergic system and mediates stress responses (59). Indeed, our observation is in accordance with a study showing that LPS treatment induced an elevation in the level of tyrosine hydroxylase mRNA and norepinephrine biosynthesis in the LC (60). Reports from our own group and others have shown that LC noradrenergic neurons are activated by stress, and optogenetic activation of these neurons induces typical anxiety-like behavior (59,61). In addition, we found that all the brain nuclei activated by LPS all involved in stressresponse and anxiety circuit (62)(63)(64)(65). Indeed, our behavioral analysis confirmed the anxiety-like behavior in the LPStreated mice.
According to our results, testis is not the only organ target of LPS-caused systemic inflammation, yet it showed very unique response to the stress compared with adrenal gland in steroidogenesis and compared with epididymis in BTB related proteins. Our study demonstrates that besides local testicular effect, LPS injection caused disturbance in the HPG axis in terms of mPOA activation and gonadotropins production. Yet, one limitation of our current study is that we provide no detailed cell-type-specific neural circuit basis for the mPOA activation, although potential upstream candidates are provided (54)(55)(56) and most of the upstream candidates were activated by LPS. In addition, our study does not provide information on the full temporal extent of this LPS-caused pathological state, as we only used two doses of LPS and all the testicular and brain effects were examined in an acute manner. Thus, further investigation using chronic LPS treatment may be useful to examine the long-term mechanism of the brain-testis axis in regulating testicular function and the possible adaptation of chronic treatment over time. Reversible manipulation of responding neurons using optogenetic or chemogenetic tools may provide more direct evidence toward our understanding of CNS involvement. Additionally, it would be useful to determine a complete brain-network mechanism, including cell-type-specific circuits, especially those circuits upstream of the hypothalamic-pituitary-gonad axis and their dynamic changes under stress.

DATA AVAILABILITY STATEMENT
The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.

ETHICS STATEMENT
The animal study was reviewed and approved by the Animal Care and Use Committees of the Shenzhen Institute of Advanced Technology (SIAT), Chinese Academy of Sciences (CAS), China.

AUTHOR CONTRIBUTIONS
LL, LW, and CW conceived and designed the project. PS collected the data. SJ, XL, QY, and BX contributed to data or analysis. LL and PS performed the analysis. PS and LL wrote the paper. All authors contributed to the article and approved the submitted version.  (B) LPS-treated mice had decreased total distance travelled, fewer entries to the center and less time in the center (data are presented as mean ± SEM, n = 9 Saline, n = 9 LPS; Student's t test, ***p < 0.001, *p < 0.05). (C) Example of EPM tracking traces of mice in the saline control and LPS groups. (D) LPS-treated mice had decreased total distance travelled (data are presented as mean ± SEM, n = 9 Saline, n = 9 LPS; Student's t test, *p < 0.05, ns, p > 0.05). (E) In open-field test, LPS at 4mg/kg caused decreases in Total distance travelled, Entries to center and Time in center, LPS at 0.4mg/kg caused decreases in Total distance travelled, Entries to center and Time in center (data are presented as Violin plot, n = 17 Saline, n = 8 0.4mg/kg LPS, n = 18 4mg/kg LPS; Kruskal-Wallis with Dunn post-hoc test, ***p < 0.001, **p < 0.01, *p < 0.05, ns, p > 0.05). (F-G) In the elevated plus maze test, 4 mg/kg LPS caused decrease in total distance travelled, 0.4mg/kg LPS caused no significant difference in Total distance travelled and a significant decrease in the ratio of Entries to Open Arms and Entries to Close Arms (data are presented as Violin plot, n = 17 Saline, n = 8, 0.4mg/kg LPS, n = 18 for 4mg/kg LPS; Kruskal-Wallis with Dunn post-hoc test, *p < 0.05, ns, p > 0.05).