Investigating the role of mitochondrial DNA D-loop variants, haplotypes, and copy number in polycystic ovary syndrome: implications for clinical phenotypes in the Chinese population

Background The presence of genetic variations in mitochondrial DNA (mtDNA) has been associated with a diverse array of diseases. The objective of this study was to examine the correlations between mtDNA D-loop, its haplotypes, and polycystic ovary syndrome (PCOS) in the Chinese population, and the associations between mtDNA D-loop and symptoms of PCOS. The study also sought to determine whether the mtDNA copy number in Chinese patients with PCOS differed from that of individuals in the control group. Methods Infertile individuals who only had tubal or male factor treatment were the focus of research by The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). mtDNA haplotypes were categorized using polymorphic D-loop sites. mtDNA D-loop, PCOS features, and mtDNA haplotypes were analyzed using R software to determine the strength of the association between the three. There are certain DNA haplotypes linked to PCOS. Microdroplet digital polymerase chain reaction (PCR) was used to determine the mtDNA copy number in a convenience sample of 168 PCOS patients and 83 controls. Results Among the research group, the majority of D-loop mutations were infrequent (frequency< 1%), with only 45 variants displaying a minimum allele frequency (MAF) of 5% or higher. No association was found between polymorphism loci in PCOS patients and body mass index (BMI). Noteworthy, C194T, 1A200G, 523delAC, and C16234T showed positive correlations with elevated LH/FSH levels. Additionally, specific polymorphic loci G207A, 16036GGins, and 16049Gins within the D-loop region of mtDNA potentially exerted a protective role in PCOS development. Conversely, no statistical significance was observed in the expression levels of C16291T and T489C. Chinese women with mtDNA haplotype A15 exhibited a decreased risk of developing PCOS. Moreover, a significant difference in mtDNA copy number was detected, with controls averaging 25.87 (21.84, 34.81), while PCOS patients had a mean of 129.91 (99.38, 168.63). Conclusion Certain mtDNA D-loop mutations and haplotypes appear to confer protection against PCOS in Chinese women. In addition, elevated mtDNA copy number may serve as an indicator during early stages of PCOS.


Background
The prevalence of polycystic ovarian syndrome (PCOS) among women of reproductive age is estimated to range between 6.3% and 8.5% (1).This condition is characterized by ovulatory dysfunction, insulin resistance (IR), and hyperinsulinemia, and a complex interplay of endocrine factors, including hyperandrogenism, irregular menstrual cycles, and the presence of polycystic ovaries as confirmed by ultrasonography (1).
The findings of hormonal studies indicated that women with PCOS exhibited distinct hormone levels in comparison to women in the control group.In particular, women with PCOS exhibited elevated levels of thyroid-stimulating hormone (TSH), total testosterone (T), and the ratio of luteinizing hormone (LH) to follicle-stimulating hormone (FSH), while experiencing decreased levels of FSH, thyroxin (T4), and progesterone (1,2).Elevated concentrations of cholesterol, low-density lipoproteins (LDL), verylow-density lipoproteins (VLDLs), and triglycerides (TGs) are commonly observed in women with PCOS, while levels of highdensity lipoproteins (HDLs) and HDL-cholesterol (HDL-C) tend to be diminished (3,4).
The etiology of PCOS remains inadequately comprehended (5).Some reports imply that dysregulation of various RNA modifications and some long non-coding RNAs (lncRNAs) dysfunctions have been observed in women with PCOS (6) (7).This poses a threat, since certain PCOS samples have also shown altered M 6 RNA modification and its regulators (5), which are involved in tumor growth (8).A similar dysregulation (GAS5 overexpression) may be seen in the expression of lncRNAs in PCOS samples (7); these lncRNAs interact with other mRNAs (9), miRNAs (10), and proteins in the cytoplasm (11).
In addition, the oxidative stress has been recently recognized as a contributing factor in the etiology of PCOS (12).The exclusion of a role for mitochondria in the pathogenesis of PCOS is not feasible, as they serve as a crucial site for the generation of reactive oxygen species (ROS) and play a central role in various metabolic processes, including the biosynthesis of steroid hormones (13,14).
MtDNA and other constituents of the mitochondria are exceptionally susceptible to damage caused by ROS due to their abundant production within the mitochondria.
The rate of mutations in the mitochondrial genome is higher compared to that in nuclear DNA, rendering it more vulnerable to oxidative damage.The aforementioned phenomenon can be attributed to its proximity to the electron transport chain (ETC) apparatus, which produces ROS, the lack of histones that safeguard DNA, and the limited efficacy of DNA repair mechanisms.The mtDNA displacement loop (D-loop) is the only non-coding region present in the mitochondrial genome.According to Stoneking (15), the hyper-variable region-1 (HVR1) (np 16024-16383) and hypervariable region-2 (HVR-2) (np 57-372) are recognized as significant sites for acquired mutations (15).The D-loop serves as the central regulatory site for the replication and transcription processes of mtDNA, as it contains the origin of replication for the leading strand and promoters for both the heavy and light strands (16).Mutations in this region may lead to an increase in the formation of cellular ROS and subsequent oxidative stress.This, in turn, can have an effect on the replication and transcription of mtDNA.A number of human disorders, such as PCOS, have been associated with sequence abnormalities in the mtDNA D-loop (17)(18)(19)(20).Undoubtedly, the presence of mutations and polymorphisms in mtDNA can give rise to compromised ATP synthesis and heightened generation of ROS, culminating in oxidative stress and impairment of mitochondrial function.The involvement of oxidative stress in the development of polycystic ovary syndrome (PCOS) is well-established, as it contributes to the occurrence of insulin resistance, hyperandrogenism, and follicular dysfunction.There is a prevailing belief that these substances have the potential to interfere with the production of energy in mitochondria and elevate levels of oxidative stress, thereby exerting an impact on the development of follicles, steroid hormone synthesis, and insulin sensitivity (21,22).
Additionally, the quantity of mtDNA copies plays a significant role in determining the level of mtDNA transcripts expressed within a cell (23).In typical physiological conditions, the cellular content of mtDNA remains relatively constant in order to meet the energy requirements necessary for cell survival (24).The alteration of mtDNA replication quantity is hypothesized to be associated with increased susceptibility to various diseases (25,26).The aforementioned modifications are instigated by environmental oxidants and the interplay between genes and the environment.
The association between changes in mtDNA copy number and the development of various diseases, such as PCOS, has been established in previous research (27).The investigation of D-loop changes and potential abnormalities in mtDNA copy number has been the subject of several studies on PCOS, yielding thoughtful findings (28).The analysis of mtDNA has been conducted to identify single nucleotide polymorphisms (SNPs), which enables the classification of distinct mitochondrial haplogroups.According to van Oven and Kayser (29), East Asians possess a significant proportion, approximately 50%, of the global mitochondrial haplogroups.These haplogroups specifically include A, B, D, G, M7, M8, M9, N9, and R9 (29).Various diseases, such as PCOS, have been associated with specific mtDNA haplogroups and variations.This association also extends to individuals of East Asian descent (30,31).Consequently, the present study focused on the Han Chinese population residing in Eastern China in order to investigate the potential association between variants and haplogroups in the mtDNA D-loop region and PCOS.The primary objective was to identify susceptibility factors specific to this ethnic group that may contribute to the development of PCOS.In addition, an examination was conducted to compare the mtDNA copy number between individuals with PCOS and a control group.This analysis aimed to offer novel perspectives on the underlying mechanisms of PCOS.

Ethical considerations
The data used in this study were obtained from publicly available databases, and therefore, ethical approval and informed consent were not applicable.However, the original studies from which the data were derived are expected to have obtained ethical approval and informed consent in accordance with the guidelines and principles outlined in the Declaration of Helsinki.

Study population and sample collection
The study population comprised a cohort of Han Chinese individuals residing in Eastern China.The inclusion criteria focused on infertile patients diagnosed with PCOS, allowing for the investigation of potential associations between variants and haplogroups in the mtDNA (mtDNA) D-loop region and PCOS.At least two of the following phenotypic characteristics are necessary for a PCOS diagnosis: the presence of 12 or more small 2-9 mm follicles in each ovary or increased ovarian volume>10 mL on ultrasound examination (2); clinical or biochemical signs of hyperandrogenism (hirsutism, acne, or serum androgen>2.64 nmol/L); and (3) polycystic ovarian morphology.Patients having a history of disease or therapy that might alter basal hormone levels (such as congenital adrenal hyperplasia, Cushing's syndrome, or androgen-secreting tumors) were also excluded.Control individuals were selected from patients experiencing infertility due solely to tubal or male factors, without any concurrent endocrine disorders leading to anovulation or elevated androgens.

Data retrieval and clinical data collection
Gene Expression Omnibus (GEO) database (https:// www.ncbi.nlm.nih.gov/geo/) and UCSC Xena database (https:// xena.ucsc.edu)were mined for information on polymorphic loci and haplotypes in the mtDNA D-loop region (32).The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as part of the clinical data gathered.FINS (U/mL) FPG (MMOL/L)/ 22.5 was used to calculate HOMA-IR (33).Subgroups of people with PCOS have been diagnosed using BMI, LH/FSH ratio, testosterone (T), and HOMA-IR values (34,35).The PCOS subgroups included both normal-weight and overweight/obese people, those with a low LH/FSH ratio and those with a high one, those with normal T levels and those with hyperandrogenemia, and those with either normal insulin metabolism or insulin resistance.

Screening and haplotyping of SNP loci in the mtDNA D-loop region
Base mutation sites and variations were identified by aligning sequences from the mtDNA D-loop region from the control region panel to the Cambridge reference sequences (36).MitoTool (37) was used for the primary haplotype analysis.In order to verify and further investigate the haplotypes that were generated, we used HaploGrep, an online mitochondrial haplotype analysis tool available through the human mitochondrial genome resource MITOMAP (https://www.mitomap.org/).Types having a quality score of 80% or above were included in the final haplotype analysis (38).If a sample's haplotype quality score was below 80%, it was either subjected to further extraction and sequencing or excluded from the study.

mtDNA copy number analysis
Individuals with PCOS and a control group were compared in order to learn more about the connection between mtDNA copy number and PCOS.The 168 PCOS patients and the 83 healthy controls all had peripheral blood samples taken.Following the manufacturer's protocol, total DNA was extracted using a commercial DNA extraction kit.Utilizing primers specific to the mitochondrial gene of interest (e.g., ND1) and a nuclear reference gene (e.g., b-actin), the mtDNA copy number was calculated.In order to determine the relative mtDNA copy number, the Ct values of the mitochondrial gene were compared to those of the nuclear reference gene.

Statistical analysis
Statistical analysis was conducted using R software.The strength of the association between mtDNA D-loop variants, haplotypes, and PCOS features was assessed using appropriate statistical tests, such as chi-square tests or logistic regression analysis.The correlation between specific polymorphic loci and clinical parameters (e.g., LH/FSH levels) was evaluated using correlation analysis.Differences in mtDNA copy number between the PCOS group and the control group were analyzed using t-tests or non-parametric tests, depending on the distribution of the data.p-values<0.05 were considered statistically significant.

Results
This study included a cohort of 422 patients diagnosed with PCOS (PCOS), with an average age of 27.53 ± 3.27 years.Additionally, a control group of 409 individuals without PCOS, with an average age of 27.90 ± 3.29 years, was also included in the study.There was no significant difference observed in age (p=0.067) and PRL levels (p=0.745) between individuals with PCOS (PCOS) and the control group.The results indicate that there was a statistically significant increase in fasting insulin levels (p<0.001), as shown in Table 1 and Figure 1.
To assess the significance of mitochondrial D-loop region variants in relation to PCOS (PCOS), a comparative analysis was conducted between the reference Cambridge Reference Sequence (RCRS) and 505 nucleotide variants.These variants encompassed 61 base insertions, 28 base deletions, and 416 base substitutions.Due to the relatively low occurrence (<1%) of D-loop variants within the study population, only 45 variants were selected for correlation analysis in this study.These variants were chosen based on a minimum allele frequency > 5% (MAF > 5%), comprising of 35 base substitutions, 8 insertions, and 2 deletions.Please refer to Table 2 and Figure 2 for further details.
The study involved the categorization of patients with PCOS (PCOS) into two groups based on their body weight, namely, normal weight body mass index (BMI) (BMI<24) and overweight/obese (BMI≥24), using the classification criteria of Chinese body mass index.The aim was to compare the variations in polymorphic loci within the D-loop region between these two groups.The analysis of Table 3 and Figure 3 revealed a lack of correlation between polymorphic loci and the level of BMI in patients diagnosed with PCOS (PCOS).
The patients diagnosed with PCOS (PCOS) were categorized into two groups based on their LH/FSH ratio: a group with a normal LH/FSH ratio (LH/FSH 2) and a group with a high LH/FSH ratio (LH/FSH>2).Subsequently, the variations in polymorphic loci within the D-loop region were compared between these two groups.The genetic variants C194T, 1A200G, 523delAC, and C16234T were found to be correlated with elevated levels of LH/ FSH.After adjusting for age and body mass index (BMI), the observed associations continued to be statistically significant.Nevertheless, following the application of false discovery rate (FDR) correction, the observed association between these polymorphic loci and high LH/FSH ratios was no longer statistically significant (refer to Table 4 and Figure 4).
The patients diagnosed with PCOS (PCOS) were categorized into two groups based on their testosterone levels: those with normal testosterone levels (T<2.64nmol/L) and those with hyperandrogenemia (T>2.64nmol/L).Subsequently, the polymorphic loci in the D-loop region were compared between these two groups.According to the data presented in Table 5 and Figure 5, there was no longer a statistically significant difference in the expression of C16291T and T489C.
As there is currently no standardized cutoff value for HOMA-IR in patients with PCOS (PCOS), the cut-off value of P7s-4.86, which represents the 75th quantile of HOMA-IR in PCOS patients, was employed as the designated threshold.However, the observed polymorphisms did not exhibit a significant correlation with HOMA-IR after adjusting for multiple testing using the FDR correction method, as indicated in Table 6 and Figure 6.
Following adjustment for age and BMI, the results of the multivariate logistic regression analysis revealed a statistically significant association between haplotype A15 and the control group (p<0.01).In order to enhance the examination of the relationship between haplotypes and PCOS, the sub-haplotypes were consolidated into their respective major haplotype bundles using the Plant tree method available in the MITOMAP database.It is important to note that all haplotypes utilized in this analysis were sourced from the aforementioned database.All haplotypes originate from the L3 type, which signifies the common ancestry of human mitochondria.These haplotypes can be further classified into two major categories, namely, M and N. The M haplogroup can be further categorized into five subhaplotypes, namely, M7, M8, M9, G, and D. The N type can be further classified into four distinct haplotypes, namely, A, N9, R, and X, which are arranged in a descending order.Among the subjects surveyed, it is observed that the R type exhibits the highest frequency of distribution, accounting for 260 out of 830 subjects.Following this, the D type is the second most prevalent, with a distribution of 187 out of 830 subjects.Lastly, the M8 type has the lowest frequency of distribution, representing 82 out of 830 subjects, as depicted in Figure 7.The M8 haplotype encompasses two primary sub-haplotypes, namely, M8a and CZ.Similarly, the G haplotype comprises two major sub-haplotypes, G2 and G3.The D haplotype consists of two prominent subhaplotypes, D4 and D5.On the other hand, the R haplotype can be further subdivided into the B and R9 haplotypes, with B, D4, R9, and D5 being more prevalent within the population.The multivariate logistic regression analysis was conducted to examine the combined haplotypes.The results showed that haplotype A15 remained significantly associated with the control population (p=0.009).Haplotype X was found to be associated only with PCOS, while haplotype F3 was associated only with the control population.These findings are presented in Figure 8 and Table 7.Comparison of clinical data between PCOS and control.
The mtDNA (mtDNA) copy number exhibited an asymmetrical distribution in both the PCOS (PCOS) and control groups.Statistical analysis using the Mann-Whitney U test revealed that the median mtDNA copy number in the control group was 25.87 (interquartile range, 21.84-34.81),while the median mtDNA copy number in the PCOS group was 129.91 (interquartile range, 99.38-168.63).The mtDNA copy number was found to be significantly higher in individuals with PCOS (PCOS) compared to the control group (p<0.001).This information is presented in Table 8 and Figure 9.

Discussion
Typically, PCOS is diagnosed using a combination of clinical criteria, hormonal profiling, and imaging methods.Ultrasonography, specifically transvaginal ultrasound, is frequently used to evaluate ovarian morphology and detect the presence of ovarian lesions and follicles (39).Despite the fact that ultrasound imaging remains the most common method for diagnosing PCOS, there is a growing interest in investigating alternative methods (40).One such strategy could involve the use of ultrasound imaging via ViT-Patch, which have demonstrated promise in imaging malignant breast cancer (41).In addition, metabolic profiling of follicular fluid by nuclear magnetic resonance (NMR) has been used to gain insight into the physiological condition of PCOS patients (42).The enhanced sensitivity of hyperpolarized xenon NMR in conjunction with metal-organic frameworks holds promise for elucidating the fundamental mechanisms and metabolic changes associated with PCOS (43).Nevertheless, understanding the fundamental molecular basis of PCOS in patients is greatly facilitated by molecular techniques, such as evaluating mtDNA alterations.Collectively, these approaches advance our understanding of PCOS and its clinical phenotypes, particularly in the Chinese population.Thus, the purpose of this research was to examine the association between PCOS and mtDNA D-loop polymorphisms, haplotypes, and copy number in the Chinese population.Our results provide light on the possible links between these genetic variables and the prevalence of PCOS.
First, there were no statistically significant variations in age or prolactin (PRL) levels between the PCOS patients and the control group, according to the study's demographic parameters.IR has been linked to PCOS, and individuals with PCOS have been shown to have considerably higher fasting insulin levels.This confirms what we already knew from looking at the pathophysiology of PCOS (Table 1; Figure 1).In a similar report, there were no statistically significant differences observed in the age and/or levels of follicle-stimulating hormone (FSH), PRL, estradiol (E2), progesterone (PRGE), or fasting glucose between patients belonging to women presenting with diagnosed hyperandrogenism, ovulatory dysfunction, and/or PCOS, as indicated by the clinical and biochemical characteristics of the participants (44).
We analyzed 505 nucleotide variations, including insertions, deletions, and substitutions, in the mtDNA D-loop region to investigate their potential role in PCOS.Due to the rarity of certain variations in the research population, we restricted our attention to 45 variants having a MAF of at least 5%.Interestingly, Table 3 and Figure 3 show that there is no association between these polymorphic loci and BMI in PCOS patients.These data implies that mtDNA D-loop mutations may not have a direct role in the association between PCOS and BMI.Our results imply that mtDNA D-loop polymorphisms may have a modest effect on BMI variance in the setting of PCOS, but it is important to keep in mind that BMI is a multifactorial characteristic impacted by both genetic and environmental factors.
Next, we looked at how the D-loop variations are connected to a major hormonal imbalance in PCOS: the LH/FSH ratio.Positive associations were found between a number of polymorphisms and elevated LH/FSH levels; these included C194T, 1A200G, 523delAC, and C16234T.After adjusting for age and BMI, however, the relationships no longer held (Table 4; Figure 4), suggesting that additional variables may affect LH/FSH levels in PCOS patients.Hormonal abnormalities in PCOS are the consequence of a dysregulation of the hypothalamic-pituitary-ovarian axis, which is itself the result of a combination of hereditary and environmental factors.
PCOS is also characterized by hyperandrogenemia, which is characterized by increased T levels.We looked at how D-loop  Correlation analysis between mtDNAD-loop region polymorphism and PCOS.Association analysis of polymorphic loci in D-loop region with BMI levels.
variants and T levels are connected in women with PCOS.The expression of C16291T and T489C polymorphisms in PCOS patients with normal T levels and those with hyperandrogenemia did not vary significantly (Table 5; Figure 5).This indicates that these polymorphisms likely do not have a causal role in the androgen excess seen in PCOS.Hyperandrogenemia in PCOS is a complicated feature impacted by a wide range of genetic, hormonal, and environmental variables.The underlying genetic factors of hyperandrogenemia in PCOS need further investigation.A comparable study conducted on women from South India Association analysis of polymorphic loci in D-loop region with LH/FSH.
revealed that the A189G and D310 SNPs exhibited the strongest association with PCOS (28).
The HOMA-IR is a commonly used tool for measuring IR, which is a prevalent symptom of PCOS.The correlation between Dloop variations and HOMA-IR levels in PCOS patients was investigated.Once the FDR multiple testing correction was used, none of the previously identified variations linked with HOMA-IR (Table 6; Figure 6), suggesting that other genetic and environmental variables may play a more substantial role in insulin resistance in PCOS.However, we found that PCOS patients had considerably higher fasting insulin levels compared to the control group.This confirms earlier studies that have linked IR to the development of PCOS (44).Hyperinsulinemia, which is exacerbated by IR, promotes androgen synthesis in the ovaries, disrupts follicular growth, and impairs glucose metabolism.Increased insulin levels exacerbate the PCOS-related hormonal abnormalities by increasing LH synthesis and decreasing sex hormone-binding globulin (SHBG) production.In a separate study conducted on Iraqi women, it was found that those with PCOS exhibited notably elevated levels of LH, LH/FSH ratio, total testosterone (TT), fasting insulin, and HOMA-IR when compared to the control group (44).
In addition, we investigated whether or not mtDNA haplotypes play a part in the development of PCOS.Haplotypes, which are groups of related genetic variants that are passed down as a unit, may provide light on the ancestry and evolutionary history of   certain populations.Major L3, M, N, and R haplotypes were found in the sample population.The haplotype A15 was shown to have a significant connection with the control group in a multivariate logistic regression analysis, suggesting a possible protective effect against PCOS (Table 7; Figure 8).In addition, the X haplotype was only discovered in PCOS individuals, indicating a possible link between the two.However, haplotype F3 was found only in the control group.These results suggest that certain haplotypes may influence PCOS risk and prevention.The complicated interaction between genetic variables and illness vulnerability is shown by our study's connections between particular haplotypes and PCOS.
We also analyzed mtDNA copy number in PCOS patients and controls in addition to variations and haplotypes.Intriguingly, we found that the mtDNA copy number in PCOS patients was considerably greater than in controls.This discovery provides more evidence that mitochondrial function and biogenesis may be altered in PCOS.Energy metabolism is largely dependent on mitochondria, and mitochondrial malfunction may cause metabolic abnormalities, oxidative stress, and cellular dysfunction.The increased mtDNA copy number in PCOS patients (Table 8; Figure 9) may represent an adaptive response to mitochondrial malfunction or a compensatory reaction to the increased energy demands associated with PCOS.Conversely, the aforementioned study conducted in India utilized RT-PCR analysis to observe a notable reduction in mtDNA copy number among individuals diagnosed with PCOS in comparison to the control group.In addition, it was observed that individuals with PCOS who carried the D310 and 189G alleles exhibited a notably reduced mtDNA copy number in comparison to those who did not carry these alleles.The carriers of the D310 mutation also exhibited a notably increased ratio of LH/FSH (28).Correspondingly, in a separate study, it was observed that the mtDNA copy numbers were significantly lower in the group of individuals with PCOS, regardless of their diabetic status (44).
Overall, our results provide new light on how mtDNA D-loop mutations, haplotypes, and copy number contribute to PCOS prevalence in the Chinese population.We detected possible protective or susceptibility effects of various haplotypes and observed significant relationships between certain variations and hormonal imbalances (e.g., LH/FSH ratio).In addition, mitochondrial dysfunction may play a role in PCOS pathogenesis because of the increased mtDNA copy number in these individuals.These results improve our knowledge of the genetic basis of PCOS and may have future applications in clinical management and individualized treatment strategies.One essential strategy for the management of this condition involves the utilization of probes capable of identifying the modified mitochondria within abnormal cells (45,46).Subsequently, these probes facilitate the targeted delivery of precise pharmaceutical agents and biological substances to the affected cells through the employment of nanocarriers (47).
To confirm and broaden these results in broader and more varied groups, further study is needed.

Conclusion
In conclusion, our research elucidates the significance of mtDNA D-loop polymorphisms, haplotypes, and copy quantity in the occurrence of PCOS in China.We found possible links between certain genetic variants and hormonal abnormalities, suggesting their participation in the pathogenesis of PCOS.In addition, the increased mtDNA copy number in PCOS suggests altered mitochondrial function and biogenesis, as shown by our results.These findings improve our comprehension of PCOS's genetic underpinnings and its clinical consequences.To confirm and generalize these results across varied groups, more research is necessary.Overall, this study deepens our understanding of PCOS   Association of A15 haplotype control.Comparative analysis of mtDNA copy number between PCOS and control.

FIGURE 5
FIGURE 5Association analysis of polymorphic loci in D-loop region with T levels.

FIGURE 6
FIGURE 6Association analysis of D-loop and region polymorphic loci with HOMA-IR.

TABLE 1
Comparison of clinical information between PCOS and control.

TABLE 2
Correlation analysis of polymorphic loci in D-loop region of mtDNAwith PCOS.

TABLE 3
Correlation analysis between polymorphic sites in D-loop region and BMI level.

TABLE 4
Association analysis of polymorphic loci in D-loop region with LH/FSH.

TABLE 5
Association analysis of polymorphic loci in D-loop region with T levels.

TABLE 6
Association analysis of polymorphic loci in D-loop region with HOMA-IR.

TABLE 7
Correlation analysis of mitochondrial haplotypes and PCOS.

TABLE 8
Comparative analysis of mtDNA copy number between PCOS and control.Mt DNA copy 129.91(98.38,168.63)25.87(21.84,34.81)<0.002 and lays the groundwork for designing individualized treatments for those who suffer from it.