Serotonin stimulates female preoptic area kisspeptin neurons via activation of type 2 serotonin receptors in mice

Background The neuroendocrine control of ovulation is orchestrated by neuronal circuits that ultimately drive the release of gonadotropin-releasing hormone (GnRH) from the hypothalamus to trigger the preovulatory surge in luteinizing hormone (LH) secretion. While estrogen feedback signals are determinant in triggering activation of GnRH neurons, through stimulation of afferent kisspeptin neurons in the rostral periventricular area of the third ventricle (RP3VKISS1 neurons), many neuropeptidergic and classical neurotransmitter systems have been shown to regulate the LH surge. Among these, several lines of evidence indicate that the monoamine neurotransmitter serotonin (5-HT) has an excitatory, permissive, influence over the generation of the surge, via activation of type 2 5-HT (5-HT2) receptors. The mechanisms through which this occurs, however, are not well understood. We hypothesized that 5-HT exerts its influence on the surge by stimulating RP3VKISS1 neurons in a 5-HT2 receptor-dependent manner. Methods We tested this using kisspeptin neuron-specific calcium imaging and electrophysiology in brain slices obtained from male and female mice. Results We show that exogenous 5-HT reversibly increases the activity of the majority of RP3VKISS1 neurons. This effect is more prominent in females than in males, is likely mediated directly at RP3VKISS1 neurons and requires activation of 5-HT2 receptors. The functional impact of 5-HT on RP3VKISS1 neurons, however, does not significantly vary during the estrous cycle. Conclusion Taken together, these data suggest that 5-HT2 receptor-mediated stimulation of RP3VKISS1 neuron activity might be involved in mediating the influence of 5-HT on the preovulatory LH surge.


Introduction
Fertility in all mammals is ultimately controlled by gonadotropin-releasing hormone (GnRH) neurons, which drive the release of anterior pituitary gland gonadotropins luteinizing hormone (LH) and follicle-stimulating hormone.In females of spontaneously ovulating species, the rise in circulating estrogen (E) concentration stimulates the mid-cycle surge in GnRH and LH secretion that causes ovulation (1).Because GnRH neurons do not express E receptor a (ERa), the receptor required for the preovulatory surge (2)(3)(4)(5)(6), these cells rely on afferent ERaexpressing neurons, the GnRH neuronal network, to relay E feedback information (7).An important part of this network for mediating preovulatory E positive feedback is the preoptic area (POA) subpopulation of kisspeptin (KISS1) neurons found in two contiguous nucleithe anteroventral periventricular nucleus and the periventricular nucleusreferred to together as the rostral periventricular area of the third ventricle (RP3V) (8,9).RP3V KISS1 neurons express ERa, undergo physiological changes in response to E positive feedback, project to GnRH neurons and can drive GnRH neuron action potential firing and surge-like LH secretion [reviewed in (10)(11)(12)].
The mechanisms through which 5-HT might regulate the LH surge, however, are not fully understood.While evidence suggests that 5-HT neurons might influence the surge via indirect mechanisms (35-37), a direct influence on the GnRH neuronal network may also be at play.Indeed, 5-HT neuronal fibers are found in the RP3V (38-40) as well as in the vicinity of GnRH neurons in the POA (41), some of which originate in the DR (42).These projections might be important seeing that 5-HT release in the hypothalamus, including the POA, increases during the surge and that neurotoxic lesion of 5-HT neuron fibers in the POA prevents the surge (28,43).At the cellular level, 5-HT can increase or decrease action potential firing and GnRH release via activation of multiple 5-HT receptors in immortalized GnRH neurons maintained in culture (44,45).Some of these effects are seen in native GnRH neurons recorded in brain slices, where, curiously, most (≈75%) female GnRH neurons are inhibited by 5-HT, via a 5-HT 1 receptor-mediated mechanism, whereas only a subset exhibits 5-HT 2 receptor-mediated increases in activity (46).The impact of 5-HT neurotransmission on the LH surge, therefore, cannot be fully accounted for by effects of this neurotransmitter on GnRH neurons.
We hypothesized here that the impact of 5-HT on the LH surge is mediated, at least in part, through regulation of RP3V KISS1 neurons upstream of GnRH neurons.Using GCaMP-based calcium imaging and electrophysiology in brain slices, we investigated the effect of 5-HT on RP3V KISS1 neuron activity and the role of 5-HT 2 receptors therein.

Animals
Mice expressing the genetically encoded calcium indicator GCaMP6f (47) in kisspeptin neurons were generated by crossing mice that express the Cre recombinase enzyme (Cre) in kisspeptin cells (Kiss1-Cre; Jackson laboratory stock #023426) (48) with mice that express Cre-dependent GCaMP6f at the ROSA26 locus (flox-STOP-GCaMP6f; Jackson laboratory stock #028865) (49).Male and female offspring heterozygous for the Kiss1-Cre and flox-STOP-GCaMP6f alleles (Kiss1-Cre::GCaMP6f) were used in calcium imaging experiments.Female mice expressing the humanized renilla green fluorescent protein (hrGFP) in kisspeptin neurons (Kiss1-hrGFP; Jackson laboratory stock #023426) (50) were used in electrophysiology experiments.All experimental mice were adults (2-6 months).Female estrous cycle stage was determined by vaginal lavage (5 µL H 2 O) taken between zeitgeber time (ZT) 1 and 3 or post-mortem (ZT2.5-5.5).Aqueous vaginal smears were stained with methylene blue and cytology examined under light microscopy to assess estrous cycle stage (51).Mice were grouphoused with littermates under controlled temperature (23 ± 2°C) and lighting (12h light/dark) conditions with ad libitum access to food and water.Mice were assigned to experiments based on their genotype, sex, and on their estrous cycle stage as needed.All experiments were approved by Kent State University's Institutional Animal Care and Use Committee.

Fixed brain slice preparation and imaging
To visualize the distribution of GCaMP6f-expressing neurons in the RP3V of male and female mice, two male and two female (one diestrus and one proestrus) Kiss1-Cre::GCaMP6f mice were deeply anesthetized via intraperitoneal injection of 3 mg/mL pentobarbital before being perfused through the heart with 4% paraformaldehyde (PFA).The brains were then dissected out and placed in 4% PFA for 1 hour before being transferred to 20% sucrose in 0.1 M phosphatebuffered saline.The brains were then cut in 3 series of coronal sections (30 mm thick) using a freezing microtome.The tissue was mounted onto superfrost charged slices, air dried, and coverslipped using an aqueous mounting medium (ProLong ™ Gold antifade mountant, Thermofisher).Epifluorescence images were taken in the RP3V region using a DMR microscope (Leica Microsystems) equipped with an ORCA-FLASH 4.0 V3 Digital CMOS (Hamamatsu, Japan), controlled by the MicroBrightField Neurolucida Software (MBF Bioscience).

Live brain slice preparation
Brain slices were prepared as previously described (52,53).Briefly, mice were decapitated following isoflurane anesthesia, and their brains quickly removed.Coronal brain slices (200 mm thick) containing the RP3V were cut using a vibrating blade microtome (HM650V, Microm International GmbH) in an ice-cold solution containing (in mM): 87 NaCl, 2.5 KCl, 25 Female mice were killed between ZT2.5 and 5.5 and males between ZT2 and 4.5.
Variations in intracellular calcium concentration ([Ca 2+ ] i ) in RP3V KISS1 neurons were estimated by measuring fluorescence changes in individual GCaMP6f-expressing RP3V neurons in slices from Kiss1-Cre::GCaMP6f mice.Slices were illuminated through a 40x immersion objective, using a light-emitting diode light source (pE300 ultra LED; CoolLED, UK) filtered for blue light excitation (460-487 nm; Semrock, USA).Epifluorescence (emission 500-546 nm; Semrock, USA) was collected using an ORCA-FLASH 4.0 LT+ CMOS camera (Hamamatsu).LED and camera were controlled and synchronized with the m-manager 1.4 software (54).After a ≥15-minute stabilization period in the recording chamber, a focal plane including several fluorescent cell bodies was chosen and acquisitions (100 ms light exposure at 2 Hz for 10-15 minutes) started.Low intensity LED illumination was used (≈0.1 to 0.8 mW) to minimize GCaMP6f photobleaching.
RP3V KISS1 neuron spontaneous action potential firing was recorded in brain slices from Kiss1-hrGFP mice.GFP-expressing RP3V neurons were visualized using brief LED illumination (excitation and emission as above) and subsequently approached with glass recording micropipettes using infrared differential interference contrast illumination.Recording micropipettes (tip resistance: 3-6 MΩ) were made with borosilicate glass (cat.#BF150-110-7.5,Sutter Instruments, USA), pulled using a Model P-1000 micropipette puller (Sutter Instruments).Action potential firing was recorded in voltage-clamp mode (no holding potential applied) in the minimally invasive cell-attached configuration (12-30 MΩ initial seal resistance).Glass micropipettes were filled with aCSF and the recording configuration was achieved by applying the lowest amount of suction required to detect spontaneous, fast, downward deflections in the current trace (spikes), which correspond to single action potentials (55).Electrical signals were recorded, filtered at 2 kHz, and digitized at 10 to 20 kHz using a double integrated patch amplifier (Sutter Instruments, USA), and acquired with the SutterPatch software (Sutter Instruments, USA).
All calcium imaging and electrophysiology experiments were performed between ZT3.5 and 10.

Drug applications
All drugs were dissolved to the appropriate stock concentration in water or in DMSO, aliquoted and stored at -20°C.Stock were diluted to working concentrations in aCSF prior to performing experiments.Final DMSO concentration never exceeded 0.1%.All drugs were bathapplied.Agonists were applied for one or two minutes after a ≥ threeminute baseline period in electrophysiology and in calcium imaging experiments.Antagonists and blockers were applied continuously to the slice for ≥ five minutes before a recording started and for the duration of the recording.In calcium imaging experiments, cell viability was routinely tested at the end of the experiments by applying the glutamate receptor agonists (S)-a-Amino-3-hydroxy-5methyl-4-isoxazolepropionic acid (AMPA; 10-20 mM) or L-glutamate (100 mM), or by raising extracellular [K + ] (+ 10 mM KCl).

Analysis
For calcium imaging, GCaMP6ffluorescence image time-series were processed in the FIJI software (56).Regions of interest (ROIs) were drawn around individual, in-focus fluorescent somata [12.1 ± 0.5 (ranging 4 to 24) and 9.2 ± 1.0 (ranging 7 to 14) ROIs per slice in females and males, respectively].Mean fluorescence intensity within each ROI was measured in each frame.Fluorescence intensity data were analyzed using scripts written in R (http://www.r-project.org/).For each ROI, normalized fluorescence was calculated as Ft F Â 100, where F is the baseline fluorescence intensity calculated as the mean fluorescence intensity over a one-minute period preceding agonist applications and F t is the fluorescence measured at any time point.Time-dependent bleaching was corrected by subtracting the normalized fluorescence obtained in an ROI that did not contain a fluorescent cell within the RP3V (empty ROI).This approach could not be used for recordings of the effect of AMPA, L-glutamate or KCl as these treatments induced very large increases in fluorescence that contaminated the empty ROI, thereby causing unacceptable distortions of traces upon subtraction.ROIs in which normalized fluorescence traces reversibly increased from baseline for a period of at least 2 minutes, around the time of agonist application, were recorded as being excited.For illustration and statistical comparisons, agonist effects were calculated as the mean normalized fluorescence over 30 seconds at the peak of the effect minus that over 30 seconds of baseline.
For electrophysiology, spikes were detected using the threshold crossing method.Spike time stamps were organized into ten-second bins and the mean firing rate calculated for each bin.To determine if 5-HT affected the spontaneous firing of RP3V KISS1 neurons, baseline firing was first measured as the mean firing rate during the two minutes preceding 5-HT application.Recordings in which the mean firing rate changed by greater than twice the standard deviation of baseline (2 × SD) during the five minutes after 5-HT first entered the bath were recorded as displaying a response to 5-HT.For statistical comparisons, firing rates were averaged over a one-minute period during baseline and at the peak of 5-HT effect.
In total, 800 GCaMP6f-expressing cells in 64 slices from 45 female mice and 55 GCaMP6f-expressing cells in 6 slices from 5 male mice were analyzed in calcium imaging experiments, while 17 GFP-expressing cells in 11 slices from 7 female mice were analyzed in electrophysiology experiments.These numbers are broken down by experiments in the results section.
Statistical analyses were performed using Prism 9.0 (GraphPad, USA).Data are reported in the text and tables as mean ± SEM, and in figures as mean ± SEM or ± 95% confidence intervals.Comparisons between two independent groups were made using the Mann-Whitney test and those between two paired groups using the paired t-test or the Wilcoxon signed rank test, as appropriate.Comparisons between multiple groups were made with the Kruskal-Wallis test with Dunn's post-tests.Comparisons of proportions were made using Fisher's exact tests.Differences were considered statistically significant for p< 0.05.

5-HT stimulates RP3V KISS1 neuron activity in a sex-dependent, but not estrous cycle-dependent, manner
The effects of 5-HT on RP3V KISS1 neuron activity were assessed using calcium imaging in brain slices from male and female Kiss1-Cre::GCaMP6f mice (Figures 1A, B).In slices from female mice, two-minute bath applications of 5-HT (10 mM) resulted in transient increases in GCaMP6f fluorescence in the majority of RP3V KISS1 neurons (Figures 1C, E).In slices obtained from male Kiss1-Cre:: GCaMP6f mice, 5-HT-induced increases in fluorescence could also be observed (Figures 1D, F), but these responses were seen much less frequently (see below).
Because 5-HT signaling may be involved in the generation of the LH surge, we examined RP3V KISS1 neuron responses to 5-HT across the estrous cycle.As illustrated in Figure 2A, 5-HT-induced increases in RP3V KISS1 neuron fluorescence could be seen in slices from diestrous, proestrous and estrous mice and were larger than those seen in slices from male mice.The proportions of RP3V KISS1 neurons excited by 5-HT were similar at all cycle stages (diestrus: 85.3%, proestrus: 87.5%, estrus: 82.0%; p > 0.18, Fisher's exact tests) and were significantly higher than in males (29.1%; p < 0.001, Fisher's exact tests; Figure 2B and Table 1).The magnitude of RP3V KISS1 neuron responses to 5-HT significantly varied across these groups, with male responses significantly smaller than those in females at any estrous cycle stage, but no statistical differences across the estrous cycle (Table 1; Figure 2C).Smaller responses to 5-HT in males versus females could not be explained by a reduced ability of male RP3V KISS1 neurons to mount changes in [Ca 2+ ] i , as responses to the glutamate receptor agonist AMPA were, in fact, larger in males than in females in those slices that were tested in this manner (Table 2).
Together, these observations indicate that RP3V KISS1 neuron responses to exogenous 5-HT areto some extentsex-dependent, being more prominent in females.Female responses, however, are not affected by the estrous cycle.The remainder of the study was conducted in brain slices from females.
These observations indicate that 5-HT primarily stimulates RP3V KISS1 neuron action potential firing.While 5-HT could also suppress firing in RP3V KISS1 neurons, this was seen much less frequently.

The effect of 5-HT on female RP3V KISS1 neuron [Ca 2+ ] i is direct
We next tested whether the effect of 5-HT is direct or mediated through the release of an intermediary factor by cells within the slice.To do this, we used a protocol in which slices from Kiss1-Cre:: GCaMP6f females were exposed to 5-HT (two minutes, 10 mM) twice at a 15-to 20-minute interval, the second 5-HT application being carried out in the presence of the voltage-gated sodium channel inhibitor tetrodotoxin (TTX; 0.5 mM) to block electrical activity in the slice.This concentration of TTX is sufficient to inhibit action potential generation in ex vivo preparations (57-59).As illustrated in Figure 4A, 5-HT-induced increases in RP3V KISS1 neuron fluorescence persisted in the presence of TTX.In control conditions, 57 out of 68 cells (83.8%) were stimulated by 5-HT.In the presence of TTX, 53 of these neurons (77.9% of the total; p = 0.51, Fisher's exact test; Figure 4B) displayed 5-HT-induced increases in normalized fluorescence.On average, 5-HT increased RP3V KISS1 neuron normalized fluorescence to a similar extent whether in the absence (2.72 ± 0.37%) or in the presence of TTX (2.34 ± 0.37%; n = 57 from 7 slices in 4 mice [2 pro-, 1 di-and 1 estrus]; p = 0.24, Wilcoxon test; Figure 4C).This finding indicates that the effect of 5-HT on RP3V KISS1 neuron activity is likely direct.
Taken together, these observations indicate that the female RP3V KISS1 neuron population expresses multiple functional excitatory 5-HT receptor subtypes.However, of these, activation The effect of exogenous 5-HT on RP3V KISS1 neuron activity across the estrous cycle and in males.  1 for details).

5-HT stimulates female RP3V KISS1 neuron activity via activation of 5-HT 2 receptors
Based on these findings, we then sought to determine the role of 5-HT 2 receptors in mediating the effect of 5-HT on RP3V KISS1 neuron activity.Using a dual 5-HT application protocol like that described above, we tested the impact of 5-HT 2 receptor antagonists on RP3V KISS1 neuron responses to 5-HT.As illustrated in Figure 6A, the antagonist ritanserin (5 mM), which prevents the LH surge in rats (34), almost completely blocked the effect of 5-HT in RP3V KISS1 neurons.69 out of 85 cells (81.2%) were stimulated by 5-HT in control conditions.In the presence of ritanserin, 31 of these (36.5% of the total; p < 0.001, Fisher's exact test) had 5-HT-induced increases in normalized fluorescence (Figure 6B).On average, 5-HT-induced changes in RP3V KISS1 neuron normalized fluorescence were substantially reduced by ritanserin (0.29 ± 0.10% versus 3.37 ± 0.32% in the absence of antagonist; n = 69 in 5 slices from 5 mice [2 pro-, 2 di-and 1 estrus]; p < 0.001, sum of signed ranks (W) = -2387, Wilcoxon test; Figure 6C).The same held true when only those cells that were excited by 5-HT in the presence of ritanserin were considered (Table 4).Similar results were obtained with the antagonist mianserin (10 mM).In its presence, only 8 out of 75 cells (10.7% versus 80.0% [60 out of 75] in control; p < 0.001, Fisher's exact test; Figure 6D) were excited by 5-HT.Moreover, 5-HT-induced increases in normalized fluorescence were significantly suppressed by mianserin (-0.53 ± 0.22% versus 2.61 ± 0.42% in the absence of antagonist; n = 60 in 6 slices from 5 mice [1 pro-, 2 di-and 2 estrus]; p < 0.001, sum of signed ranks (W) = -1718, Wilcoxon test; Figure 6E), even when only cells stimulated by 5-HT in the presence of the antagonist were considered (Table 4).
Together with the results obtained using 5-HT receptor agonists, these data indicate that the effect of 5-HT on RP3V KISS1 neurons isfor the most partmediated by 5-HT 2 receptors, likely through the combined effect of 5-HT 2 A and 5-HT 2 C receptor activation.

Discussion
We report here using GCaMP-based [Ca 2+ ] i imaging and electrophysiology that exogenous 5-HT stimulates the activity of a majority of RP3V KISS1 neurons in female mice.This effect is observed in higher proportions of RP3V KISS1 neuronsand with larger magnitudesin females than in males.In females, however, the effect of 5-HT does not significantly vary between estrous cycle stages.Lastly, we find that 5-HT-induced excitations are likely mediated directly at RP3V KISS1 neurons and that these effects require activation of 5-HT 2 receptors.Together, these observations suggest that stimulation of RP3V KISS1 neurons might be a mechanism through which 5-HT influences the LH surge in rodents.
A large proportion of female RP3V KISS1 neurons (> 80%) exhibited increases in [Ca 2+ ] i in response to 5-HT in brain slices a includes only those cells that responded to 5-HT.
from Kiss1-Cre::GCaMP6f mice.In slices from Kiss1-hrGFP mice, 5-HT caused an increase in action potential firing in > 50% of RP3V KISS1 neurons.Increases in [Ca 2+ ] i induced by 5-HT likely result from opening of voltage-gated Ca 2+ channels in response to action potential firing and/or to subthreshold membrane depolarization, from G-protein-dependent Ca 2+ release from internal stores, or from a combination thereof (10,61).As the cell-attached patch-clamp recording configuration does not give access to subthreshold membrane potential fluctuationsnor to changes in [Ca 2+ ] ithe proportions of neurons that responded to 5-HT applications with elevations in [Ca 2+ ] i and in firing rates cannot be directly compared.Interestingly, 5-HT suppressed firing in a small subset (≈ 10%) of RP3V KISS1 neurons in electrophysiology experiments.It is possible that such responses were not seen with our [Ca 2+ ] i imaging because, at least under our recording conditions, this approach does not effectively resolve inhibitions in those cells that display low resting activity levels (10,61).Nevertheless, our observation that 5-HT stimulated activity in a majority of RP3V KISS1 neurons is in line with a recent report that 5-HT increases firing in arcuate kisspeptin (ARC KISS1 ) neurons (62), suggesting that stimulation by 5-HT is a common feature of hypothalamic kisspeptin neurons.Somewhat contrastingly, only a small proportion of adult female GnRH neurons (< 40%) are stimulated by 5-HT, whereas most (≈ 75%) are inhibited (46).The stimulatory effect of 5-HT on RP3V KISS1 neuron [Ca 2+ ] i was largely resistant to blockade of action potential firing, suggesting that responses to 5-HT were mediated primarily through action potential-independent VGCC opening and/or

B C A
The effect of 5-HT on female RP3V KISS1 neuron activity is direct.(A) 5-HT was applied twice to brain slices from female mice, at 15-to-20-minute intervals.The first application (control; left) was carried out in the absence of drugs whereas the second was conducted in the continuous presence of tetrodotoxin (TTX; right) to block action potentials.Traces are means (black) ± 95% confidence intervals (grey) and include those RP3V KISS1 neurons that were stimulated by 5-HT upon the first application (n = 57).Blue shading indicates the timing of 5-HT bath applications.(B) Similar proportions of RP3V KISS1 neurons were stimulated by 5-HT in the presence and in the absence of TTX.Numbers in bars are sample sizes (excited cells/total cells).(C) The peak magnitude of the 5-HT effect was similar in the presence and in the absence of TTX.Only RP3V KISS1 neurons excited by 5-HT upon the first application were included in this analysis.The number above the bars is the sample size.mobilization of intracellular Ca 2+ stores.This also reveals that RP3V KISS1 neuron [Ca 2+ ] i responses to 5-HT were independent on electrical activity within the brain slice and, therefore, resulted from direct 5-HT actions at the kisspeptin neurons.Moreover, RP3V KISS1 neurons displayed excitatory responses to 5-HT 2A , 5-HT 2C , 5-HT 3 , 5-HT 4 , 5-HT 6 and 5-HT 7 receptor activation.This reveals that RP3V KISS1 neurons may express multiple functional 5-HT receptor subtypes.However, activation of 5-HT 2C andto a lesser extent -5-HT 2A receptors most closely mimicked the stimulatory effect of 5-HT.Importantly, the stimulatory effect of 5-HT was blocked by the 5-HT 2 receptor antagonists ritanserin and mianserin.Together, these observations indicate that 5-HT  3 for details).stimulates RP3V KISS1 neuron activity by acting at 5-HT 2 receptors, including 5-HT 2A and 5-HT 2C receptors.5-HT is also reported to stimulate the activity of ARC KISS1 neurons, but this is mostly mediated by 5-HT 4 receptors (62).The two main populations of hypothalamic kisspeptin neurons may, therefore, have different functional 5-HT receptor make-ups.Nevertheless, our observations in RP3V KISS1 neurons are in line with previous reports that ritanserin prevents the LH surge in rats (33,34), and that activation of 5-HT 2A and 5-HT 2C receptors restores the LH surge in DR-lesioned female rats (26).Seeing the pattern and pharmacology of RP3V KISS1 neuron responses to 5-HTand, as discussed above, that 5-HT may primarily inhibit female GnRH neurons ( 46)we propose that the stimulatory effect of 5-HT we report here might be involved in mediating the previously reported excitatory influence of 5-HT neurotransmission on the LH surge (27)(28)(29)(30)(32)(33)(34).5-HT responses were found in a substantially and significantly lower proportion of RP3V KISS1 neurons in males (≈ 35%) than in females.Further, those male RP3V KISS1 neurons that were stimulated by 5-HT displayed much smaller responses than their female counterparts.Although we cannot fully rule out that a proportion of the GCaMP6fexpressing cells that we recorded in slices from male mice were RP3V neurons that do not, in fact, express Kiss1 (10, 63-65), this suggests that 5-HT excitatory effects in RP3V KISS1 neurons display some degree of sexdependence.As the LH surge mechanism, including many aspects of the RP3V KISS1 → GnRH neural circuit, is sexually differentiated in rodents (66)(67)(68)(69), this observation suggests that 5-HT signaling in RP3V KISS1 neurons might be part of the mechanism activating these cells for the surge.On the other hand, we find that the impact of 5-HT on RP3V KISS1 neuron activity did not significantly change between estrous cycle stages, with similar proportions of neurons stimulated and similar response magnitude.This suggests that regulation of 5-HT 2 receptor function in RP3V KISS1 neurons might not be a mechanism through which these cells are specifically activated for the proestrous surge.Rather, this suggests that 5-HT might have a permissive effect on RP3V KISS1 neuron activity as it does on the surge in vivo (15).On the other hand, 5-HT content and turnover in the hypothalamus, including the POA, might fluctuate around the time of the proestrous surge in a diurnal manner (28,70,71).Whether 5-HT release in the vicinity of RP3V KISS1 neurons fluctuates in a time-and estrous cycle-dependent manner is unknown but would be anticipated to affect RP3V KISS1 neuron activity.
5-HT-containing fibers are detected within the RP3V in male and female rats (38-40), but it is unknown whether these fibers are in the vicinity of kisspeptin neurons.The origin of these fibers is also unknown.They may originate in the DR as lesions of this area, more so than lesions of the medial raphe nucleus, prevent the LH surge (23)(24)(25)(26).Curiously, electrical stimulation of the DR fails at altering the LH surge in proestrous rats (24), whereas this manipulation can evoke LH secretion in E-replaced OVX rats (72) and result in a subtle prolongation of LH secretion evoked by stimulation of the POA (73).DR neurons project directly to POA GnRH neurons (42); whether they project to RP3V KISS1 neurons will need to be established by tract-tracing experiments.It should be noted that 5-HT transporter-immunoreactive fibers are seen in close apposition to ARC KISS1 neurons (62).As ARC KISS1 neurons might be involved in regulating the LH surge (74)(75)(76)(77)(78), it is possible that these fibers contribute to the influence of 5-HT on the surge.As discussed above, the pharmacology of ARC KISS1 neuron responses to 5-HT does not match that of the influence of 5-HT on the surge, however.
Lastly, the type of information that 5-HT neurons relay to the GnRH neuronal network to regulate the surge is unknown.5-HT may mediate the permissive effect of the metabolic hormone leptin on the hypothalamic-pituitary-gonadal axis (79) and, indeed, the DR is involved in many aspects of energy balance (80).Additionally, 5-HT signaling may be involved in regulating circadian rhythms and responses to stressors (81-83), which also affect LH secretion and the surge (20,21).Further studies will be required to determine the precise contribution of changes in 5-HT neurotransmission in regulating the surge and whether this involves RP3V KISS1 neurons.
In conclusion, the findings reported here regarding the stimulatory effect of 5-HT signaling on RP3V KISS1 neuron activity contribute to our understanding of the brain circuits that control ovulation in females.and Dr. Aleisha Moore for helping with taking pictures of GCaMP6f endogenous fluorescence in fixed tissue.
FIGURE 1 5-HT stimulates RP3V KISS1 neuron activity.(A, B) Low magnification (10X) epifluorescence images of endogenous GCaMP6f fluorescence in fixed coronal slices (30 mm thick) including the RP3V from a diestrous female (A) and a male (B) mouse.Insets i and ii are 20X magnification images of areas framed (dashed lines) in the low magnification images.Similar GCaMP6f-expressing cell distribution was observed in two different animals of each sex.White arrowheads indicate individual GCaMP6f-expressing neurons.3V: third ventricle.Scale bars are 200 mm (50 mm in i and ii).(C, D) Epifluorescence images (40X magnification, average projection of 240 frames) of endogenous GCaMP6f fluorescence in acute coronal slices (200 mm thick) including the RP3V obtained from an estrous female (C) and a male (D) mouse.Ovals and numbers indicate individual regions of interest (ROIs) each including a single cell.Dashed lines delineate the border of the 3V.Scale bars 25 mm.(E, F) Normalized fluorescence traces for the ROIs delineated in C and D. Blue shading indicates the timing of 5-HT bath applications.Please note the difference in scale for normalized fluorescence in female (E) versus male traces (F).
(A) 5-HT-induced changes in RP3V KISS1 neuron normalized fluorescence across the estrous cycle and in males.Traces are means (black) ± 95% confidence intervals (grey).(B) Similar proportions of RP3V KISS1 neurons were stimulated by 5-HT across the estrous cycle.This proportion was significantly lower in males.Numbers in bars are sample sizes (excited cells/total cells).(C) Peak 5-HT effect did not vary as a function of estrous cycle stage but was significantly smaller in males.Numbers in bars are sample sizes.Letters indicate results of statistical tests [Fisher's exact tests in (B) Kruskal-Wallis and Dunn's post-tests in (C)].Bars with different letters are significantly different (p < 0.05; see Table

3 5-
FIGURE 3 5-HT effects on female RP3V KISS1 neuron action potential firing.(A, B) Example traces and corresponding rate-meters illustrating the excitatory (A) and inhibitory (B) effects of 5-HT on female RP3V KISS1 neuron firing.Blue shading indicates the timing of 5-HT bath applications.(C) Proportions of RP3V KISS1 neurons that showed increased, decreased or no change in firing in response to 5-HT.(D) In those cells that were excited by 5-HT, the increase in firing was statistically significant.**p < 0.01 paired t-test.Numbers above bars are sample sizes.

5
FIGURE 5 Responses of female RP3V KISS1 neurons to excitatory 5-HT receptor agonists.(A, B) Average responses of female RP3V KISS1 neurons stimulated by 5-HT 2A (A) and 5-HT 2C (B) receptor agonists.Colored shading indicates the timing of agonist applications.Traces are means (black) ± 95% confidence intervals (grey).(C) Proportions of female RP3V KISS1 neurons stimulated by 5-HT receptor agonists.Numbers in bars are sample sizes (excited cells/ total cells).(D) Magnitude of the effect of different 5-HT receptor agonists.Only RP3V KISS1 neurons that responded to agonists were included in this analysis.Numbers above bars are sample sizes.Letters above bars represent the results of statistical tests [Fisher's exact tests in (C) and Kruskal-Wallis and Dunn's post-tests in (D)].Bars that do not share a letter are significantly different (p < 0.05, see Table3for details).

6 5-
FIGURE 6 5-HT2 receptors mediate the effect of 5-HT on female RP3V KISS1 neuron activity.(A) 5-HT was applied twice to female brain slices, at 15-to-20minute intervals.The first application (control; left) was carried out in the absence of drugs whereas the second was conducted in the continuous presence of ritanserin (right), a 5-HT2 receptor antagonist.Light blue shading indicates the timing of 5-HT bath applications.Traces are means (black) ± 95% confidence intervals (grey).(B) Proportions of RP3V KISS1 neurons stimulated by 5-HT significantly decreased in the presence of ritanserin.***p < 0.001, Fisher's exact test.Numbers in bars are sample sizes (excited cells/total cells).(C) Ritanserin significantly decreased the peak magnitude of the 5-HT effect.***p < 0.001, Wilcoxon signed rank test.Number above bars is the sample size.(D) Mianserin significantly reduced the proportions of RP3V KISS1 neurons stimulated by 5-HT.***p < 0.001, Fisher's exact test.Numbers in bars are sample sizes (excited cells/total cells).(E) The peak magnitude of the 5-HT effect was significantly suppressed by mianserin.***p < 0.001, Wilcoxon signed rank test.Number above bars is the sample size.Only RP3V KISS1 neurons that responded to 5-HT upon the first bath application were included in the analyses displayed in (C, E).

TABLE 1 RP3V
KISS1neuron responses to 5-HT across the female estrous cycle and in males.Wallis test was used to test for statistical differences across all 4 groups (males, di-, pro-and estrous females).b the 16 male neurons that responded were in 4 slices from 3 different males.

TABLE 2
Response of female and male RP3V KISS1 neurons to stimulation by AMPA.

TABLE 3 RP3V
KISS1neuron excitatory responses to 5-HT receptor agonists in female mice.Wallis test was used to test for statistical differences across the effect of all 6 agonists (TCB-2, WAY161503, SR57227, cisapride, WAY208466 and AS19).
b RP3V KISS1 cells showing excitatory responses to SR57227, WAY208466 and to AS19 were found in 6 slices from 4 mice, in 4 slices from 3 mice and in 3 slices from 3 mice, respectively.

TABLE 4
Excitatory effects of 5-HT in the presence of 5-HT 2 receptor antagonists ritanserin or mianserin.